@article {RN2456,
	title = {Editorial: Archaea in the Environment: Views on Archaeal Distribution, Activity, and Biogeography},
	journal = {Frontiers in Microbiology},
	volume = {12},
	year = {2021},
	pages = {667596},
	type = {Journal Article},
	doi = {10.3389/fmicb.2021.667596},
	url = {https://app.dimensions.ai/details/publication/pub.1136340014 http://dx.doi.org/10.3389/fmicb.2021.667596},
	author = {Teske, Andreas and Amils, Ricardo and Ram{\'\i}rez, Gustavo A. and Reysenbach, Anna-Louise}
}
@article {RN2496,
	title = {Interactions between temperature and energy supply drive microbial communities in hydrothermal sediment},
	journal = {Communications Biology},
	volume = {4},
	number = {1},
	year = {2021},
	pages = {1006},
	type = {Journal Article},
	doi = {10.1038/s42003-021-02507-1},
	url = {https://app.dimensions.ai/details/publication/pub.1140634439 https://www.nature.com/articles/s42003-021-02507-1.pdf},
	author = {Lagostina, Lorenzo and Frandsen, S{\o}s and MacGregor, Barbara J. and Glombitza, Clemens and Deng, Longhui and Fiskal, Annika and Li, Jiaqi and Doll, Mechthild and Geilert, Sonja and Schmidt, Mark and Scholz, Florian and Bernasconi, Stefano Michele and J{\o}rgensen, Bo Barker and Hensen, Christian and Teske, Andreas and Lever, Mark Alexander}
}
@article {RN2451,
	title = {Microbial Communities Under Distinct Thermal and Geochemical Regimes in Axial and Off-Axis Sediments of Guaymas Basin},
	journal = {Frontiers in Microbiology},
	volume = {12},
	year = {2021},
	pages = {633649},
	type = {Journal Article},
	doi = {10.3389/fmicb.2021.633649},
	url = {https://app.dimensions.ai/details/publication/pub.1135332514 https://fjfsdata01prod.blob.core.windows.net/articles/files/633649/pubmed-zip/.versions/1/.package-entries/fmicb-12-633649/fmicb-12-633649.pdf?sv=2018-03-28\&sr=b\&sig=WKLBqhvZrkcj2oO6X4DtspOriwBM},
	author = {Teske, Andreas and Wegener, Gunter and Chanton, Jeffrey P. and White, Dylan and MacGregor, Barbara and Hoer, Daniel and de Beer, Dirk and Zhuang, Guangchao and Saxton, Matthew A. and Joye, Samantha B. and Lizarralde, Daniel and Soule, S. Adam and Ruff, S. Emil}
}
@article {RN2355,
	title = {Filamentous Giant Beggiatoaceae from the Guaymas Basin Are Capable of both Denitrification and Dissimilatory Nitrate Reduction to Ammonium},
	journal = {Applied and Environmental Microbiology},
	volume = {84},
	number = {15},
	year = {2018},
	pages = {e02860-17},
	type = {Journal Article},
	doi = {10.1128/AEM.02860-17},
	url = {http://aem.asm.org/content/84/15/e02860-17.abstract},
	author = {Schutte, Charles A. and Teske, Andreas and MacGregor, Barbara J. and Salman-Carvalho, Verena and Lavik, Gaute and Hach, Philipp and de Beer, Dirk}
}
@article {ISI:000368893500001,
	title = {{Microbial Communities in Methane- and Short Chain Alkane-Rich Hydrothermal Sediments of Guaymas Basin}},
	journal = {FRONTIERS IN MICROBIOLOGY},
	volume = {7},
	year = {2016},
	month = {jan},
	type = {Article},
	abstract = {The hydrothermal sediments of Guaymas Basin, an active spreading center in the Gulf of California (Mexico), are rich in porewater methane, short-chain alkanes, sulfate and sulfide, and provide a model system to explore habitat preferences of microorganisms, including sulfate-dependent, methane- and short chain alkane-oxidizing microbial communities. In this study, hot sediments (above 60 degrees C) covered with sulfur-oxidizing microbial mats surrounding a hydrothermal mound (termed {\textquoteleft}{\textquoteleft}Mat Mound{\{}{\textquoteright}{\textquoteright}{\}}) were characterized by porewater geochemistry of methane, C-2-C-6 short-chain alkanes, sulfate, sulfide, sulfate reduction rate measurements, in situ temperature gradients, bacterial and archaeal 16S rRNA gene clone libraries and V6 tag pyrosequencing. The most abundantly detected groups in the Mat mound sediments include anaerobic methane-oxidizing archaea of the ANME-1 lineage and its sister Glade ANME-1Guaymas, the uncultured bacterial groups SEEP-SRB2 within the Deltaproteobacteria and the separately branching HotSeep-1 Group; these uncultured bacteria are candidates for sulfate-reducing alkane oxidation and for sulfate-reducing syntrophy with ANME archaea. The archaeal dataset indicates distinct habitat preferences for ANME-1, ANME-1-Guaymas, and ANME-2 archaea in Guaymas Basin hydrothermal sediments. The bacterial groups SEEP-SRB2 and HotSeep-1 co-occur with ANME-1 and ANME-1Guaymas in hydrothermally active sediments underneath microbial mats in Guaymas Basin. We propose the working hypothesis that this mixed bacterial and archaeal community catalyzes the oxidation of both methane and short-chain alkanes, and constitutes a microbial community signature that is characteristic for hydrothermal and/or cold seep sediments containing both substrates.},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {1664-302X},
	doi = {10.3389/fmicb.2016.00017},
	author = {Dowell, Frederick and Cardman, Zena and Dasarathy, Srishti and Kellermann, Matthias Y and Lipp, Julius S and Ruff, S Emil and Biddle, Jennifer F and McKay, Luke J and MacGregor, Barbara J and Lloyd, Karen G and Albert, Daniel B and Mendlovitz, Howard and Hinrichs, Kai-Uwe and Teske, Andreas}
}
@article {ISI:000371481100019,
	title = {{Thermal and geochemical influences on microbial biogeography in the hydrothermal sediments of Guaymas Basin, Gulf of California}},
	journal = {ENVIRONMENTAL MICROBIOLOGY REPORTS},
	volume = {8},
	number = {1},
	year = {2016},
	month = {feb},
	pages = {150{\textendash}161},
	type = {Article},
	abstract = {Extreme thermal gradients and compressed metabolic zones limit the depth range of microbial colonization in hydrothermally active sediments at Guaymas Basin. We investigated the physicochemical characteristics of this ecosystem and their influence on microbial community structure. Temperature-related trends of C-13 values of methane and dissolved inorganic carbon from 36 sediment cores suggest in situ thermal limits for microbial anaerobic methane oxidation and organic carbon re-mineralization near 80 degrees C and 100 degrees C respectively. Temperature logging probes deposited in hydrothermal sediments for 8 days demonstrate substantial thermal fluctuations of up to 25 degrees C. Putative anaerobic methanotroph (ANME) populations dominate the archaeal community, transitioning from ANME-1 archaea in warm surficial sediments towards ANME-1 Guaymas archaea as temperatures increase downcore. Since ANME archaea performing anaerobic oxidation of methane double on longer time scales (months) compared with relatively rapid in situ temperature fluctuations (hours to days), we conclude that ANME archaea possess a high tolerance for short-term shifts in the thermal regime.},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {1758-2229},
	doi = {10.1111/1758-2229.12365},
	author = {McKay, Luke and Klokman, Vincent W and Mendlovitz, Howard P and LaRowe, Douglas E and Hoer, Daniel R and Albert, Daniel and Amend, Jan P and Teske, Andreas}
}
@article {ISI:000331366700001,
	title = {{Microbial habitat connec ivity across spatial scales and hydrothermal temperature gradients at Guaymas Basin}},
	journal = {FRONTIERS IN MICROBIOLOGY},
	volume = {4},
	year = {2013},
	month = {jul},
	type = {Article},
	abstract = {The Guaymas Basin (Gulf of California) hydrothermal vent area is known as a dynamic and hydrothermally vented sedimentary system, where the advection and production of a variety of different metabolic substrates support a high microbial diversity and activity in the seafloor. The main objective of our study was to explore the role of temperature and other environmental factors on community diversity, such as the presence of microbial mats and seafloor bathymetry within one hydrothermally vented field of 200 x 250 m dimension. In this field, temperature increased strongly with sediment depth reaching the known limit of life within a few decimeters. Potential sulfate reduction rate as a key community activity parameter was strongly affected by in situ temperature and sediment depth, declining from high rates of 1-5 jimol m1-1 d-1 at the surface to the detection limit below 5 cm sediment depth, despite the presence of sulfate and hydrocarbons. Automated Ribosomal lntergenic Spacer Analysis yielded a high-resolution fingerprint of the dominant members of the bacterial community. Our analyses showed strong temperature and sediment depth effects on bacterial cell abundance and Operational Taxonomic Units (OTUs) number, both declining by more than one order of magnitude below the top 5 cm of the sediment surface. Another fraction of the variation in diversity and community structure was explained by differences in the local bathymetry and spatial position within the vent field. Nevertheless, more than 80{\%} of all detected OTUs were shared among the different temperature realms and sediment depths, after being classified as cold (T {\textless} 10 C), medium (10 C {\textless} T {\textless} 40 C) or hot (T {\textgreater} 40 C) temperature conditions, with significant OTU overlap with the richer surface communities. Overall, this indicates a high connectivity of benthic bacterial habitats in this dynamic and heterogeneous marine ecosystem influenced by strong hydrothermalism.},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {1664-302X},
	doi = {10.3389/fmicb.2013.00207},
	author = {Meyer, Stefanie and Wegener, Gunter and Lloyd, Karen G and Teske, Andreas and Boetius, Antje and Ramette, Alban}
}
@article {ISI:000326614000008,
	title = {{Sulfide oxidation, nitrate respiration, carbon acquisition, and electron transport pathways suggested by the draft genome of a single orange Guaymas Basin Beggiatoa (Cand. Maribeggiatoa) sp filament}},
	journal = {MARINE GENOMICS},
	volume = {11},
	year = {2013},
	month = {sep},
	pages = {53{\textendash}65},
	type = {Article},
	abstract = {A near-complete draft genome has been obtained for a single vacuolated orange Beggiatoa (Cand. Maribeggiatoa) filament from a Guaymas Basin seafloor microbial mat, the third relatively complete sequence for the Beggiatoaceae. Possible pathways for sulfide oxidation; nitrate respiration; inorganic carbon fixation by both Type II RuBisCO and the reductive tricarboxylic add cycle; acetate and possibly formate uptake; and energy-generating electron transport via both oxidative phosphorylation and the Rnf complex are discussed here. A role in nitrite reduction is suggested for an abundant orange cytochrome produced by the Guaymas strain; this has a possible homolog in Beggiatoa (Cand. Isobeggiatoa) sp. PS, isolated from marine harbor sediment, but not Beggiatoa alba B18LD, isolated from a freshwater rice field ditch. Inferred phylogenies for the Calvin-Benson-Bassham (CBB) cycle and the reductive (rTCA) and oxidative (TCA) tricarboxylic acid cycles suggest that genes encoding succinate dehydrogenase and enzymes for carboxylation and/or decarboxylation steps (including RuBisCO) may have been introduced to (or exported from) one or more of the three genomes by horizontal transfer, sometimes by different routes. Sequences from the two marine strains are generally more similar to each other than to sequences from the freshwater strain, except in the case of RuBisCO: only the Guaymas strain encodes a Type H enzyme, which (where studied) discriminates less against oxygen than do Type I RuBisCOs. Genes subject to horizontal transfer may represent key steps for adaptation to factors such as oxygen and carbon dioxide concentration, organic carbon availability, and environmental variability. (C) 2013 Elsevier B.V. All rights reserved.},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {1874-7787},
	doi = {10.1016/j.margen2013.08.001},
	author = {MacGregor, Barbara J and Biddle, Jennifer F and Harbort, Christopher and Matthysse, Ann G and Teske, Andreas}
}
@article {ISI:000314891500014,
	title = {{Why Orange Guaymas Basin Beggiatoa spp. Are Orange: Single-Filament-Genome-Enabled Identification of an Abundant Octaheme Cytochrome with Hydroxylamine Oxidase, Hydrazine Oxidase, and Nitrite Reductase Activities}},
	journal = {APPLIED AND ENVIRONMENTAL MICROBIOLOGY},
	volume = {79},
	number = {4},
	year = {2013},
	month = {feb},
	pages = {1183{\textendash}1190},
	type = {Article},
	abstract = {Orange, white, and yellow vacuolated Beggiatoaceae filaments are visually dominant members of microbial mats found near sea floor hydrothermal vents and cold seeps, with orange filaments typically concentrated toward the mat centers. No marine vacuolate Beggiatoaceae are yet in pure culture, but evidence to date suggests they are nitrate-reducing, sulfide-oxidizing bacteria. The nearly complete genome sequence of a single orange Beggiatoa ({\{}{\textquoteright}{\textquoteright}{\}}Candidatus Maribeggiatoa{\{}{\textquoteright}{\textquoteright}{\}}) filament from a microbial mat sample collected in 2008 at a hydrothermal site in Guaymas Basin (Gulf of California, Mexico) was recently obtained. From this sequence, the gene encoding an abundant soluble orange-pigmented protein in Guaymas Basin mat samples (collected in 2009) was identified by microcapillary reverse-phase high-performance liquid chromatography (HPLC) nano-electrospray tandem mass spectrometry (mu LC-MS-MS) of a pigmented band excised from a denaturing polyacrylamide gel. The predicted protein sequence is related to a large group of octaheme cytochromes whose few characterized representatives are hydroxylamine or hydrazine oxidases. The protein was partially purified and shown by in vitro assays to have hydroxylamine oxidase, hydrazine oxidase, and nitrite reductase activities. From what is known of Beggiatoaceae physiology, nitrite reduction is the most likely in vivo role of the octaheme protein, but future experiments are required to confirm this tentative conclusion. Thus, while present-day genomic and proteomic techniques have allowed precise identification of an abundant mat protein, and its potential activities could be assayed, proof of its physiological role remains elusive in the absence of a pure culture that can be genetically manipulated.},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {0099-2240},
	doi = {10.1128/AEM.02538-12},
	author = {MacGregor, Barbara J and Biddle, Jennifer F and Siebert, Jason R and Staunton, Eric and Hegg, Eric L and Matthysse, Ann G and Teske, Andreas}
}
@article {ISI:000302950700011,
	title = {{Anaerobic oxidation of methane at different temperature regimes in Guaymas Basin hydrothermal sediments}},
	journal = {ISME JOURNAL},
	volume = {6},
	number = {5},
	year = {2012},
	month = {may},
	pages = {1018{\textendash}1031},
	type = {Article},
	abstract = {Anaerobic oxidation of methane (AOM) was investigated in hydrothermal sediments of Guaymas Basin based on delta C-13 signatures of CH4, dissolved inorganic carbon and porewater concentration profiles of CH4 and sulfate. Cool, warm and hot in-situ temperature regimes (15-20 degrees C, 30-35 degrees C and 70-95 degrees C) were selected from hydrothermal locations in Guaymas Basin to compare AOM geochemistry and 16S ribosomal RNA (rRNA), mcrA and dsrAB genes of the microbial communities. 16S rRNA gene clone libraries from the cool and hot AOM cores yielded similar archaeal types such as Miscellaneous Crenarchaeotal Group, Thermoproteales and anaerobic methane-oxidizing archaea (ANME)-1; some of the ANME-1 archaea formed a separate 16S rRNA lineage that at present seems to be limited to Guaymas Basin. Congruent results were obtained by mcrA gene analysis. The warm AOM core, chemically distinct by lower porewater sulfide concentrations, hosted a different archaeal community dominated by the two deep subsurface archaeal lineages Marine Benthic Group D and Marine Benthic Group B, and by members of the Methanosarcinales including ANME-2 archaea. This distinct composition of the methane-cycling archaeal community in the warm AOM core was confirmed by mcrA gene analysis. Functional genes of sulfate-reducing bacteria and archaea, dsrAB, showed more overlap between all cores, regardless of the core temperature. 16S rRNA gene clone libraries with Euryarchaeota-specific primers detected members of the Archaeoglobus clade in the cool and hot cores. A V6-tag high-throughput sequencing survey generally supported the clone library results while providing high-resolution detail on archaeal and bacterial community structure. These results indicate that AOM and the responsible archaeal communities persist over a wide temperature range. The ISME Journal (2012) 6, 1018-1031; doi:10.1038/ismej.2011.164; published online 17 November 2011},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {1751-7362},
	doi = {10.1038/ismej.2011.164},
	author = {Biddle, Jennifer F and Cardman, Zena and Mendlovitz, Howard and Albert, Daniel B and Lloyd, Karen G and Boetius, Antje and Teske, Andreas}
}
@article {ISI:000208863600280,
	title = {{Microbial communities at the borehole observatory on the Costa Rica Rift flank (Ocean Drilling Program Hole 896A)}},
	journal = {FRONTIERS IN MICROBIOLOGY},
	volume = {3},
	year = {2012},
	type = {Article},
	abstract = {The microbiology of subsurface, hydrothermally influenced basaltic crust flanking midocean ridges has remained understudied, due to the difficulty in accessing the subsurface environment. The instrumented boreholes resulting from scientific ocean drilling offer access to samples of the formation fluids circulating through oceanic crust. We analyzed the phylogenetic diversity of bacterial communities of fluid and microbial mat samples collected in situ from the observatory at Ocean Drilling Program Hole 896A, drilled into similar to 6.5 million-year-old basaltic crust on the flank of the Costa Rica Rift in the equatorial Pacific Ocean. Bacterial 16S rRNA gene sequences recovered from borehole fluid and from a microbial mat coating the outer surface of the fluid port revealed both unique and shared phylotypes. The dominant bacterial clones from both samples were related to the autotrophic, sulfur-oxidizing genus Thiomicrospira. Both samples yielded diverse gamma- and alphaproteobacterial phylotypes, as well as members of the Bacteroidetes, Planctomycetes, and Verrucomicrobia. Analysis of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) genes (cbbL and cbbM) from the sampling port mat and from the borehole fluid demonstrated autotrophic carbon assimilation potential for in situ microbial communities; most cbbL genes were related to those of the sulfur-oxidizing genera Thioalkalivibrio and Thiomicrospira, and cbbM genes were affiliated with uncultured phylotypes from hydrothermal vent plumes and marine sediments. Several 16S rRNA gene phylotypes from the 896A observatory grouped with phylotypes recovered from seawater-exposed basalts and sulfide deposits at inactive hydrothermal vents, but there is little overlap with hydrothermally influenced basaltic boreholes 1026B and U1301A on the Juan de Fuca Ridge flank, suggesting that site-specific characteristics of Hole 896A (i.e., seawater mixing into borehole fluids) affect the microbial community composition.},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {1664-302X},
	doi = {10.3389/fmicb.2012.00232},
	author = {Nigro, Lisa M and Harris, Kate and Orcutt, Beth N and Hyde, Andrew and Clayton-Luce, Samuel and Becker, Keir and Teske, Andreas}
}
@article {ISI:000299052400011,
	title = {{Thermophilic anaerobic oxidation of methane by marine microbial consortia}},
	journal = {ISME JOURNAL},
	volume = {5},
	number = {12},
	year = {2011},
	month = {dec},
	pages = {1946{\textendash}1956},
	type = {Article},
	abstract = {The anaerobic oxidation of methane (AOM) with sulfate controls the emission of the greenhouse gas methane from the ocean floor. AOM is performed by microbial consortia of archaea (ANME) associated with partners related to sulfate-reducing bacteria. In vitro enrichments of AOM were so far only successful at temperatures {\textless}= 25 degrees C; however, energy gain for growth by AOM with sulfate is in principle also possible at higher temperatures. Sequences of 16S rRNA genes and core lipids characteristic for ANME as well as hints of in situ AOM activity were indeed reported for geothermally heated marine environments, yet no direct evidence for thermophilic growth of marine ANME consortia was obtained to date. To study possible thermophilic AOM, we investigated hydrothermally influenced sediment from the Guaymas Basin. In vitro incubations showed activity of sulfate-dependent methane oxidation between 5 and 70 degrees C with an apparent optimum between 45 and 60 degrees C. AOM was absent at temperatures {\textgreater}= 75 degrees C. Long-term enrichment of AOM was fastest at 50 degrees C, yielding a 13-fold increase of methane-dependent sulfate reduction within 250 days, equivalent to an apparent doubling time of 68 days. The enrichments were dominated by novel ANME-1 consortia, mostly associated with bacterial partners of the deltaproteobacterial HotSeep-1 cluster, a deeply branching phylogenetic group previously found in a butane-amended 60 degrees C-enrichment culture of Guaymas sediments. The closest relatives (Desulfurella spp.; Hippea maritima) are moderately thermophilic sulfur reducers. Results indicate that AOM and ANME archaea could be of biogeochemical relevance not only in cold to moderate but also in hot marine habitats. The ISME Journal (2011) 5, 1946-1956; doi: 10.1038/ismej.2011.77; published online 23 June 2011},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {1751-7362},
	doi = {10.1038/ismej.2011.77},
	author = {Holler, Thomas and Widdel, Friedrich and Knittel, Katrin and Amann, Rudolf and Kellermann, Matthias Y and Hinrichs, Kai-Uwe and Teske, Andreas and Boetius, Antje and Wegener, Gunter}
}
