@article {Beaudoin2016,
	title = {{Impact of protists on a hydrocarbon-degrading bacterial community from deep-sea Gulf of Mexico sediments: A microcosm study}},
	journal = {Deep-Sea Research. Part II: Topical Studies in Oceanography},
	volume = {129},
	year = {2016},
	pages = {350{\textendash}359},
	abstract = {In spite of significant advancements towards understanding the dynamics of petroleum hydrocarbon degrading microbial consortia, the impacts (direct or indirect via grazing activities) of bacterivorous protists remain largely unknown. Microcosm experiments were used to examine whether protistan grazing affects the petroleum hydrocarbon degradation capacity of a deep-sea sediment microbial community from an active Gulf of Mexico cold seep. Differences in n-alkane content between native sediment microcosms and those treated with inhibitors of eukaryotes were assessed by comprehensive two-dimensional gas chromatography following 30{\textendash}90 day incubations and analysis of shifts in microbial community composition using small subunit ribosomal RNA gene clone libraries. More biodegradation was observed in microcosms supplemented with eukaryotic inhibitors. SSU rRNA gene clone libraries from oil-amended treatments revealed an increase in the number of proteobacterial clones (particularly $\gamma$-proteobacteria) after spiking sediments with diesel oil. Bacterial community composition shifted, and degradation rates increased, in treatments where protists were inhibited, suggesting protists affect the hydrocarbon degrading capacity of microbial communities in sediments collected at this Gulf of Mexico site.},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {0967-0645},
	doi = {10.1016/j.dsr2.2014.01.007},
	url = {http://www.sciencedirect.com/science/article/pii/S0967064514000216},
	author = {Beaudoin, D J and Carmichael, C A and Nelson, R K and Reddy, C M and Teske, A P and Edgcomb, V P}
}
@article {Kormas2015,
	title = {{Inter-comparison of the potentially active prokaryotic communities in the halocline sediments of Mediterranean deep-sea hypersaline basins}},
	journal = {Extremophiles},
	volume = {19},
	year = {2015},
	pages = {949{\textendash}960},
	abstract = {The sediment microbiota of the Mediterranean deep-sea anoxic hypersaline basins (DHABs) are understudied relative to communities in the brines and halocline waters. In this study, the active fraction of the prokaryotic community in the halocline sediments of L{\textquoteright} Atalante, Urania, and Discovery DHABs was investigated based on extracted total RNA and 454 pyrosequencing of the 16S rRNA gene. Bacterial and archaeal communities were different in the sediments underlying the halocline waters of the three habitats, reflecting the unique chemical settings of each basin. The relative abundance of unique operational taxonomic units (OTUs) was also different between deep-sea control sediments and sediments underlying DHAB haloclines, suggesting adaptation to the steep DHAB chemical gradients. Only a few OTUs were affiliated to known bacterial halophilic and/or anaerobic groups. Many OTUs, including some of the dominant ones, were related to aerobic taxa. Archaea were detected only in few halocline samples, with lower OTU richness relative to Bacteria, and were dominated by taxa associated with methane cycling. This study suggests that, while metabolically active prokaryotic communities appear to be present in sediments underlying the three DHABs investigated, their diversity and activity are likely to be more reduced in sediments underlying the brines.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	author = {Kormas, K and Pachiadaki, M and Karayanni, H and Leadbetter, E R and Bernhard, J M and Edgcomb, V P}
}
