@article {Schimak2012,
	title = {{Simultaneous 16S and 18S rRNA fluorescence in situ hybridization (FISH) on LR White sections demonstrated in Vestimentifera (Siboglinidae) tubeworms}},
	journal = {Acta Histochemica},
	volume = {114},
	number = {2},
	year = {2012},
	pages = {122{\textendash}130},
	abstract = {Traditional morphological identification of invertebrate marine species is limited in early life history stages for many taxa. In this study, we demonstrate, by example of Vestimentiferan tubeworms (Siboglinidae, Polychaeta), that the simultaneous fluorescence in situ hybridization (FISH) of both eukaryotic host and bacterial symbiont cells is possible on a single semi-thin (1~$μ$m) section. This allows the identification of host specimens to species level as well as offering visualization of bacteria distributed within the host tissue. Previously published 18S rRNA host-specific oligonucleotide probes for Riftia pachyptila, Tevnia jerichonana and a newly designed Oasisia alvinae probe, as well as a 16S rRNA probe targeting symbionts found in all host species, were applied. A number of standard fixation and hybridization parameters were tested and optimized for the best possible signal intensity and cellular resolution. Ethanol conserved samples embedded in LR White low viscosity resin yielded the best results with regard to both signal intensity and resolution. We show that extended storage times of specimens does not affect the quality of signals attained by FISH and use our protocol to identify morphologically unidentifiable tubeworm individuals from a small data set, conforming to previous findings in succession studies of the Siboglinidae family.},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {0065-1281},
	doi = {10.1016/j.acthis.2011.03.008},
	url = {http://dx.doi.org/10.1016/j.acthis.2011.03.008},
	author = {Schimak, M P and Toenshoff, E R and Bright, M}
}
@article {Bright2010,
	title = {{Epizooic metazoan meiobenthos associated with tubeworm and mussel aggregations from cold seeps of the northern Gulf of Mexico}},
	journal = {Deep-Sea Research. Part II: Topical Studies in Oceanography},
	volume = {57},
	number = {21-23},
	year = {2010},
	pages = {1982{\textendash}1989},
	abstract = {The abundance and higher taxonomic composition of epizooic metazoan meiobenthic communities associated with mussel and tubeworm aggregations of hydrocarbon seeps at Green Canyon, Atwater Valley, and Alaminos Canyon in depths between 1400 and 2800 m were studied and compared to the infaunal community of non-seep sediments nearby. Epizooic meiofaunal abundances of associated meiobenthos living in tubeworm bushes and mussel beds at seeps were extremely low (usually {\textless}100 ind. 10 cm-2), similar to epizooic meiofauna at deep-sea hydrothermal vents, and the communities were composed primarily of nematodes, copepods, ostracods, and halacarids. In contrast, epizooic meiobenthic abundance is lower than previous studies have reported for infauna from seep sediments. Interestingly, non-seep sediments contained higher abundances and higher taxonomic diversity than epizooic seep communities, although in situ primary production is restricted to seeps.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0645},
	doi = {10.1016/j.dsr2.2010.05.003},
	url = {http://dx.doi.org/10.1016/j.dsr2.2010.05.003},
	author = {Bright, M and Plum, C and Riavitz, L A and Arbizu, P M and Cordes, E E and Gollner, S}
}
@article {Roberts2007a,
	title = {{Alvin explores the deep northern Gulf of Mexico Slope}},
	journal = {EOS, Transactions, American Geophysical Union},
	volume = {88},
	number = {35},
	year = {2007},
	pages = {341{\textendash}342},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {2324-9250},
	doi = {10.1029/2007EO350001},
	url = {http://dx.doi.org/10.1029/2007EO350001},
	author = {Roberts, H and Carney, R and Kupchik, M and Fisher, C R and Nelson, Kim and Becker, E and Goehring, E and Lessard-Pilon, S and Telesnicki, G and Bernard, B and Brooks, J and Bright, M and Cordes, E and Hourdez, S and Hunt, J and Shedd, W and Boland, G and Joye, S and Samarkin, V and Bernier, M and Bowles, M and MacDonald, I and Niemann, H and Petersen, C and Morrison, C and Potter, J}
}
@article {Gollner2007,
	title = {{Tubeworm-associated permanent meiobenthic communities from two chemically different hydrothermal vent sites on the East Pacific Rise}},
	journal = {Marine Ecology Progress Series},
	volume = {337},
	year = {2007},
	pages = {39{\textendash}49},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {0171-8630},
	doi = {10.3354/meps337039},
	url = {http://dx.doi.org/10.3354/meps337039},
	author = {Gollner, S and Zekeley, J and Govenar, B and Le Bris, N and Nemeschkal, H L and Fisher, C R and Bright, M}
}
@article {Gollner2006,
	title = {{Benthic copepod communities associated with tubeworm and mussel aggregations on the East Pacific Rise}},
	journal = {Cahiers de Biologie Marine},
	volume = {47},
	number = {4},
	year = {2006},
	pages = {397{\textendash}402},
	abstract = {Variability in the structure of the meiobenthic copepod communities was studied among three vent sites on the East Pacific Rise. One of the sites was dominated by the mussel Bathymodiolus thermophilus, and the other two of the sites were dominated by the tubeworm Riftia pachyptila. In addition to the differences in the dominant megafauna, the maximum temperatures were much lower at the mussel-dominated sites, and the temperature to sulfide ratio was different between the tubeworm-dominated sites. A total of 22 vent endemic siphonostomatoid copepod species and 5 harpacticoid copepod species were identified among the three sites. Copepod abundance was low at all sites (},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {0007-9723},
	author = {Gollner, S and Zekely, Julia and Van Dover, C L and Govenar, B and Le Bris, N and Nemeschkal, H L and Bright, M}
}
@article {Nussbaumer2006,
	title = {{Horizontal endosymbiont transmission in hydrothermal vent tubeworms}},
	journal = {Nature},
	volume = {441},
	number = {7091},
	year = {2006},
	month = {may},
	pages = {345{\textendash}348},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {0028-0836},
	doi = {10.1038/nature04793},
	author = {Nussbaumer, A D and Fisher, C R and Bright, M}
}
@article {Zekely2006,
	title = {{Hydrothermal vent meiobenthos associated with mytilid mussel aggregations from the Mid-Atlantic Ridge and the East Pacific Rise}},
	journal = {Deep-Sea Research. Part I: Oceanographic Research Papers},
	volume = {53},
	number = {8},
	year = {2006},
	pages = {1363{\textendash}1378},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {0967-0637},
	doi = {10.1016/j.dsr.2006.05.010},
	url = {http://dx.doi.org/10.1016/j.dsr.2006.05.010},
	author = {Zekely, J and Van Dover, C L and Nemeschkal, H L and Bright, M}
}
@article {Salerno2005,
	title = {{Characterization of symbiont populations in life-history stages of mussels from chemosynthetic environments}},
	journal = {Biological Bulletin},
	volume = {208},
	number = {2},
	year = {2005},
	pages = {145{\textendash}155},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {0006-3185},
	url = {http://www.biolbull.org/cgi/content/abstract/208/2/145},
	author = {Salerno, J L and Macko, S A and Hallam, S J and Bright, M and Won, Y -J and McKiness, Z and Van Dover, C L}
}
@article {Pflugfelder2005,
	title = {{The color of the trophosome: elemental sulfur distribution in the endosymbionts of Riftia pachyptila (Vestimentifera; Siboglinidae)}},
	journal = {Marine Biology},
	volume = {146},
	number = {5},
	year = {2005},
	pages = {895{\textendash}901},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {0025-3162},
	doi = {10.1007/s00227-004-1500-x},
	url = {http://dx.doi.org/10.1007/s00227-004-1500-x},
	author = {Pflugfelder, B and Fisher, C R and Bright, M}
}
@article {Bright2000,
	title = {{An autoradiographic examination of carbon fixation, transfer and utilization in the Riftia pachyptila symbiosis}},
	journal = {Marine Biology},
	volume = {136},
	number = {4},
	year = {2000},
	month = {may},
	pages = {621{\textendash}632},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {0025-3162},
	doi = {10.1007/s002270050722},
	author = {Bright, M and Keckeis, H and Fisher, C R}
}
@article {Scott1999,
	title = {{Carbon dioxide use by chemoautotrophic endosymbionts of hydrothermal vent vestimentiferans: affinities for carbon dioxide, absence of carboxysomes, and delta C-13 values}},
	journal = {Marine Biology},
	volume = {135},
	number = {1},
	year = {1999},
	month = {oct},
	pages = {25{\textendash}34},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0025-3162},
	doi = {10.1007/s002270050597},
	author = {Scott, K M and Bright, M and Macko, S A and Fisher, C R}
}
@article {Scott1998,
	title = {{The burden of independence: Inorganic carbon utilization strategies of the sulphur chemoautotrophic hydrothermal vent isolate Thiomicrospira crunogena and the symbionts of hydrothermal vent and cold seep vestimentiferans}},
	journal = {Cahiers de Biologie Marine},
	volume = {39},
	number = {3-4},
	year = {1998},
	pages = {379{\textendash}381},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {0007-9723},
	author = {Scott, K M and Bright, M and Fisher, C R}
}
