@article {Meyer2013,
	title = {{Microbiological characterization of post-eruption {\textquotedblleft}snowblower{\textquotedblright} vents at Axial Seamount, Juan de Fuca Ridge}},
	journal = {Frontiers in Microbiology},
	volume = {4},
	year = {2013},
	pages = {153},
	abstract = {Microbial processes within the subseafloor can be examined during the ephemeral and uncommonly observed phenomena known as snowblower venting. Snowblowers are characterized by the large quantity of white floc that is expelled from the seafloor following mid-ocean ridge eruptions. During these eruptions, rapidly cooling lava entrains seawater and hydrothermal fluids enriched in geochemical reactants, creating a natural bioreactor that supports a subseafloor microbial {\textquotedblleft}bloom.{\textquotedblright} Previous studies hypothesized that the eruption-associated floc was made by sulfide-oxidizing bacteria; however, the microbes involved were never identified. Here we present the first molecular analysis combined with microscopy of microbial communities in snowblower vents from samples collected shortly after the 2011 eruption at Axial Seamount, an active volcano on the Juan de Fuca Ridge. We obtained fluid samples and white flocculent material from active snowblower vents as well as orange flocculent material found on top of newly formed lava flows. Both flocculent types revealed diverse cell types and particulates when examined by phase contrast and scanning electron microscopy (SEM). Distinct archaeal and bacterial communities were detected in each sample type through Illumina tag sequencing of 16S rRNA genes and through sequencing of the sulfide oxidation gene, soxB. In fluids and white floc, the dominant bacteria were sulfur-oxidizing Epsilonproteobacteria and the dominant archaea were thermophilic Methanococcales. In contrast, the dominant organisms in the orange floc were Gammaproteobacteria and Thaumarchaeota Marine Group I. In all samples, bacteria greatly outnumbered archaea. The presence of anaerobic methanogens and microaerobic Epsilonproteobacteria in snowblower communities provides evidence that these blooms are seeded by subseafloor microbes, rather than from microbes in bottom seawater. These eruptive events thus provide a unique opportunity to observe subseafloor microbial communities.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.3389/fmicb.2013.00153},
	author = {Meyer, J L and Akerman, N H and Proskurowski, G and Huber, J A}
}
@article {Lang2012,
	title = {{Microbial utilization of abiogenic carbon and hydrogen in a serpentinite-hosted system}},
	journal = {Geochimica et Cosmochimica Acta},
	volume = {92},
	year = {2012},
	pages = {82{\textendash}99},
	abstract = {Mantle rocks exposed on the seafloor constitute a highly reactive chemical and thermal system, in which interaction with seawater to produce serpentinite has major consequences for lithospheric cooling, global geochemical cycles, and microbial activity. Serpentinite-hosted hydrothermal activity is exemplified by the Lost City Hydrothermal Field (30{\textdegree}N, Mid-Atlantic Ridge) where fluid{\textendash}rock reactions in the underlying ultramafic rocks result in high concentrations of abiotic hydrogen, methane, C2+ alkanes, and formate. Such systems have been proposed as possible analogs to the Early Earth environments that gave rise to the first biochemical pathways. Thus, characterizing the local microbial communities and their potential link with abiogenic compounds is of particular significance. Here we demonstrate that in active carbonate chimneys where microbial sulfate reduction is important, up to 50{\%} of the microbial biomass is synthesized from mantle carbon. Conversely, mantle carbon contributes only \~{}10{\%} of the biomass in areas with minimal sulfate reduction. We attribute this difference to greater incorporation of formate or methane by the dominant microbial species, the Lost City Methanosarcinales, in locations where sulfate reducers are able to facilitate this assimilation. The ability of autotrophic communities at Lost City to capitalize on the steady stream of chemical products resulting from serpentinization reactions and to utilize abiogenic mantle carbon lend credence to the hypothesis that early biosynthetic pathways could have developed in similar environments.},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	doi = {10.1016/j.gca.2012.06.006},
	url = {http://dx.doi.org/10.1016/j.gca.2012.06.006},
	author = {Lang, S Q and Fruh-Green, G L and Bernasconi, S M and Lilley, M D and Proskurowski, G and Mehay, S and Butterfield, D A}
}
@article {Mottl2011,
	title = {{Chemistry of hot springs along the Eastern Lau Spreading Center}},
	journal = {Geochimica et Cosmochimica Acta},
	volume = {75},
	number = {4},
	year = {2011},
	pages = {1013{\textendash}1038},
	abstract = {The Eastern Lau Spreading Center (ELSC) is the southernmost part of the back-arc spreading axis in the Lau Basin, west of the Tonga trench and the active Tofua volcanic arc. Over its 397-km length it exhibits large and systematic changes in spreading rate, magmatic/tectonic processes, and proximity to the volcanic arc. In 2005, we collected 81 samples of vent water from six hydrothermal fields along the ELSC. The chemistry of these waters varies both within and between vent fields, in response to changes in substrate composition, temperature and pressure, pH, water/rock ratio, and input from magmatic gases and subducted sediment. Hot-spring temperatures range from 229{\textdegree} to 363~{\textdegree}C at the five northernmost fields, with a general decrease to the south that is reversed at the Mariner field. The southernmost field, Vai Lili, emitted water at up to 334~{\textdegree}C in 1989 but had a maximum venting temperature of only 121~{\textdegree}C in 2005, due to waning activity and admixture of bottom seawater into the subseafloor plumbing system. Chloride varies both within fields and from one field to another, from a low of 528~mmol/kg to a high of 656~mmol/kg, and may be enriched by phase separation and/or leaching of Cl from the rock. Concentrations of the soluble elements K, Rb, Cs, and B likewise increase southward as the volcanic substrate becomes more silica-rich, especially on the Valu Fa Ridge. Iodine and $δ$7Li increase southward, and $δ$11B decreases as B increases, apparently in response to increased input from subducted sediment as the arc is approached. Species that decrease southward as temperature falls are Si, H2S, Li, Na/Cl, Fe, Mn, and 87Sr/86Sr, whereas pH, alkalinity, Ca, and Sr increase. Oxygen isotopes indicate a higher water/rock ratio in the three systems on Valu Fa Ridge, consistent with higher porosity in more felsic volcanic rocks. Vent waters at the Mariner vent field on the Valu Fa Ridge are significantly hotter, more acid and metal-rich, less saline, and richer in dissolved gases and other volatiles, including H2S, CO2, and F, than the other vent fields, consistent with input of magmatic gases. The large variations in geologic and geophysical parameters produced by back-arc spreading along the ELSC, which exceed those along mid-ocean ridge spreading axes, produce similar large variations in the composition of vent waters, and thus provide new insights into the processes that control the chemistry of submarine hot springs.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0016-7037},
	doi = {10.1016/j.gca.2010.12.008},
	url = {http://dx.doi.org/10.1016/j.gca.2010.12.008},
	author = {Mottl, M J and Seewald, J S and Wheat, C G and Tivey, M K and Michael, P J and Proskurowski, G and McCollom, T M and Reeves, E and Sharkey, J and You, C F and Chan, L H and Pichler, T}
}
@article {Proskurowski2008,
	title = {{Stable isotopic evidence in support of active microbial methane cycling in low-temperature diffuse flow vents at 9 degrees 50{\textquoteright}N East Pacific Rise}},
	journal = {Geochimica et Cosmochimica Acta},
	volume = {72},
	number = {8},
	year = {2008},
	pages = {2005{\textendash}2023},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {0016-7037},
	doi = {10.1016/j.gca.2008.01.025},
	url = {http://dx.doi.org/10.1016/j.gca.2008.01.025},
	author = {Proskurowski, G and Lilley, M D and Olson, E J}
}
@article {Proskurowski2006,
	title = {{Low temperature volatile production at the Lost City Hydrothermal Field: evidence from a hydrogen stable isotope geothermometer}},
	journal = {Chemical Geology},
	volume = {229},
	number = {4},
	year = {2006},
	pages = {331{\textendash}343},
	keywords = {AUV ABE (Autonomous Benthic Explorer), HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0009-2541},
	doi = {10.1016/j.chemgeo.2005.11.005},
	url = {http://dx.doi.org/10.1016/j.chemgeo.2005.11.005},
	author = {Proskurowski, G and Lilley, M D and Kelley, D S and Olson, E J}
}
@article {Kelley2005a,
	title = {{A serpentinite-hosted ecosystem: The lost city hydrothermal field}},
	journal = {Science},
	volume = {307},
	number = {5714},
	year = {2005},
	pages = {1428{\textendash}1434},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {0036-8075},
	doi = {10.1126/science.1102556},
	url = {http://dx.doi.org/10.1126/science.1102556},
	author = {Kelley, D S and Karson, J A and Fruh-Green, G L and Yoerger, D R and Shank, T M and Butterfield, D A and Hayes, J M and Schrenk, M O and Olson, E J and Proskurowski, G and Jakuba, M V and Bradley, A M and Larson, B I and Ludwig, K A and Glickson, D A and Buckman, K and Bradley, A S and Brazelton, W J and Roe, K and Elend, M J and Delacour, A and Bernasconi, S M and Lilley, M D and Baross, J A and Summons, R T and Sylva, S P}
}
@article {Fruh-Green2003,
	title = {{30,000 years of hydrothermal activity at the Lost City vent field}},
	journal = {Science},
	volume = {301},
	number = {5632},
	year = {2003},
	pages = {495{\textendash}498},
	issn = {0036-8075},
	doi = {10.1126/science.1085582},
	url = {http://dx.doi.org/10.1126/science.1085582},
	author = {Fruh-Green, G L and Kelley, D S and Bernasconi, S M and Karson, J A and Ludwig, K A and Butterfield, D A and Boschi, C and Proskurowski, G}
}
@article {VonDamm2003,
	title = {{Extraordinary phase separation and segregation in vent fluids from the southern East Pacific Rise}},
	journal = {Earth and Planetary Science Letters},
	volume = {206},
	number = {3-4},
	year = {2003},
	month = {feb},
	pages = {365{\textendash}378},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {0012-821X},
	doi = {10.1016/S0012-821X(02)01081-6},
	url = {http://dx.doi.org/10.1016/S0012-821X(02)01081-6},
	author = {Von Damm, K L and Lilley, M D and Shanks, W C and Brockington, M and Bray, A M and O{\textquoteright}Grady, K M and Olson, E J and Graham, A and Proskurowski, G and Party, SouEPR Science}
}
