@article {ISI:000368893500001,
	title = {{Microbial Communities in Methane- and Short Chain Alkane-Rich Hydrothermal Sediments of Guaymas Basin}},
	journal = {FRONTIERS IN MICROBIOLOGY},
	volume = {7},
	year = {2016},
	month = {jan},
	type = {Article},
	abstract = {The hydrothermal sediments of Guaymas Basin, an active spreading center in the Gulf of California (Mexico), are rich in porewater methane, short-chain alkanes, sulfate and sulfide, and provide a model system to explore habitat preferences of microorganisms, including sulfate-dependent, methane- and short chain alkane-oxidizing microbial communities. In this study, hot sediments (above 60 degrees C) covered with sulfur-oxidizing microbial mats surrounding a hydrothermal mound (termed {\textquoteleft}{\textquoteleft}Mat Mound{\{}{\textquoteright}{\textquoteright}{\}}) were characterized by porewater geochemistry of methane, C-2-C-6 short-chain alkanes, sulfate, sulfide, sulfate reduction rate measurements, in situ temperature gradients, bacterial and archaeal 16S rRNA gene clone libraries and V6 tag pyrosequencing. The most abundantly detected groups in the Mat mound sediments include anaerobic methane-oxidizing archaea of the ANME-1 lineage and its sister Glade ANME-1Guaymas, the uncultured bacterial groups SEEP-SRB2 within the Deltaproteobacteria and the separately branching HotSeep-1 Group; these uncultured bacteria are candidates for sulfate-reducing alkane oxidation and for sulfate-reducing syntrophy with ANME archaea. The archaeal dataset indicates distinct habitat preferences for ANME-1, ANME-1-Guaymas, and ANME-2 archaea in Guaymas Basin hydrothermal sediments. The bacterial groups SEEP-SRB2 and HotSeep-1 co-occur with ANME-1 and ANME-1Guaymas in hydrothermally active sediments underneath microbial mats in Guaymas Basin. We propose the working hypothesis that this mixed bacterial and archaeal community catalyzes the oxidation of both methane and short-chain alkanes, and constitutes a microbial community signature that is characteristic for hydrothermal and/or cold seep sediments containing both substrates.},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {1664-302X},
	doi = {10.3389/fmicb.2016.00017},
	author = {Dowell, Frederick and Cardman, Zena and Dasarathy, Srishti and Kellermann, Matthias Y and Lipp, Julius S and Ruff, S Emil and Biddle, Jennifer F and McKay, Luke J and MacGregor, Barbara J and Lloyd, Karen G and Albert, Daniel B and Mendlovitz, Howard and Hinrichs, Kai-Uwe and Teske, Andreas}
}
@article {ISI:000331366700001,
	title = {{Microbial habitat connec ivity across spatial scales and hydrothermal temperature gradients at Guaymas Basin}},
	journal = {FRONTIERS IN MICROBIOLOGY},
	volume = {4},
	year = {2013},
	month = {jul},
	type = {Article},
	abstract = {The Guaymas Basin (Gulf of California) hydrothermal vent area is known as a dynamic and hydrothermally vented sedimentary system, where the advection and production of a variety of different metabolic substrates support a high microbial diversity and activity in the seafloor. The main objective of our study was to explore the role of temperature and other environmental factors on community diversity, such as the presence of microbial mats and seafloor bathymetry within one hydrothermally vented field of 200 x 250 m dimension. In this field, temperature increased strongly with sediment depth reaching the known limit of life within a few decimeters. Potential sulfate reduction rate as a key community activity parameter was strongly affected by in situ temperature and sediment depth, declining from high rates of 1-5 jimol m1-1 d-1 at the surface to the detection limit below 5 cm sediment depth, despite the presence of sulfate and hydrocarbons. Automated Ribosomal lntergenic Spacer Analysis yielded a high-resolution fingerprint of the dominant members of the bacterial community. Our analyses showed strong temperature and sediment depth effects on bacterial cell abundance and Operational Taxonomic Units (OTUs) number, both declining by more than one order of magnitude below the top 5 cm of the sediment surface. Another fraction of the variation in diversity and community structure was explained by differences in the local bathymetry and spatial position within the vent field. Nevertheless, more than 80{\%} of all detected OTUs were shared among the different temperature realms and sediment depths, after being classified as cold (T {\textless} 10 C), medium (10 C {\textless} T {\textless} 40 C) or hot (T {\textgreater} 40 C) temperature conditions, with significant OTU overlap with the richer surface communities. Overall, this indicates a high connectivity of benthic bacterial habitats in this dynamic and heterogeneous marine ecosystem influenced by strong hydrothermalism.},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {1664-302X},
	doi = {10.3389/fmicb.2013.00207},
	author = {Meyer, Stefanie and Wegener, Gunter and Lloyd, Karen G and Teske, Andreas and Boetius, Antje and Ramette, Alban}
}
