@article {ISI:000325142700005,
	title = {{Growth kinetics and energetics of a deep-sea hyperthermophilic methanogen under varying environmental conditions}},
	journal = {ENVIRONMENTAL MICROBIOLOGY REPORTS},
	volume = {5},
	number = {5},
	year = {2013},
	month = {oct},
	pages = {665{\textendash}671},
	type = {Article},
	abstract = {{A hyperthermophilic deep-sea methanogen, Methanocaldococcus strain JH146, was isolated from 26 degrees C hydrothermal fluid at Axial Volcano to model high temperature methanogenesis in the subseafloor. Emphasis was placed on defining growth kinetics, cell yields and growth energy demand (GE) across a range of conditions. The organism uses H-2 and CO2 as its sole carbon and energy sources. At various temperatures, pHs, and chlorinities, its growth rates and cell yields co-varied while GE remained uniform at 1.69x10(-11) J cell(-1)s(-1)+/- 0.68x10(-11) J cell(-1)s(-1) (s.d.},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {1758-2229},
	doi = {10.1111/1758-2229.12065},
	author = {Eecke, Helene C Ver and Akerman, Nancy H and Huber, Julie A and Butterfield, David A and Holden, James F}
}
@article {ISI:000331246100002,
	title = {{Phylogenetic diversity and functional gene patterns of sulfur-oxidizing subseafloor Epsilonproteobacteria in diffuse hydrothermal vent fluids}},
	journal = {FRONTIERS IN MICROBIOLOGY},
	volume = {4},
	year = {2013},
	month = {jul},
	type = {Article},
	abstract = {Microorganisms throughout the dark ocean use reduced sulfur compounds for chemolithoautotrophy. In many deep-sea hydrothermal vents, sulfide oxidation is quantitatively the most important chemical energy source for microbial metabolism both at and beneath the seafloor. In this study, the presence and activity of vent endemic Epsilonproteobacteria was examined in six low-temperature diffuse vents over a range of geochemical gradients from Axial Seamount, a deep-sea volcano in the Northeast Pacific. PCR primers were developed and applied to target the sulfur oxidation soxB gene of Epsilonproteobacteria. soxB genes belonging to the genera Sulfurimonas and Sulfurovum are both present and expressed at most diffuse vent sites, but not in background seawater. Although Sulfurovum-like sox8 genes were detected in all fluid samples, the RNA profiles were nearly identical among the vents and suggest that Sulfurirnonas-like species are the primary Epsilonproteobacteria responsible for actively oxidizing sulfur via the Sox pathway at each vent. Community patterns of subseafloor Epsdonproteobactena 16S rRNA genes were best matched to methane concentrations in vent fluids, as well as individual vent locations, indicating that both geochemistry and geographical isolation play a role in structuring subseafloor microbial populations. The data show that in the subseafloor at Axial Seamount, Epsilonproteobacteria are expressing the soxB gene and that microbial patterns in community distribution are linked to both vent location and chemistry.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1664-302X},
	doi = {10.3389/fmicb.2013.00185},
	author = {Akerman, Nancy H and Butterfield, David A and Huber, Julie A}
}
