@article {ISI:000328605200015,
	title = {{Nitrate-based niche differentiation by distinct sulfate-reducing bacteria involved in the anaerobic oxidation of methane}},
	journal = {ISME JOURNAL},
	volume = {8},
	number = {1},
	year = {2014},
	month = {jan},
	pages = {150{\textendash}163},
	type = {Article},
	abstract = {Diverse associations between methanotrophic archaea (ANME) and sulfate-reducing bacterial groups (SRB) often co-occur in marine methane seeps; however, the ecophysiology of these different symbiotic associations has not been examined. Here, we applied a combination of molecular, geochemical and Fluorescence in situ hybridization (FISH) coupled to nanoscale secondary ion mass spectrometry (FISH-NanoSIMS) analyses of in situ seep sediments and methane-amended sediment incubations from diverse locations (Eel River Basin, Hydrate Ridge and Costa Rican Margin seeps) to investigate the distribution and physiology of a newly identified subgroup of the Desulfobulbaceae (seepDBB) found in consortia with ANME-2c archaea, and compared these with the more commonly observed associations between the same ANME partner and the Desulfobacteraceae (DSS). FISH analyses revealed aggregates of seepDBB cells in association with ANME-2 from both environmental samples and laboratory incubations that are distinct in their structure relative to co-occurring ANME/DSS consortia. ANME/seepDBB aggregates were most abundant in shallow sediment depths below sulfide-oxidizing microbial mats. Depth profiles of ANME/seepDBB aggregate abundance revealed a positive correlation with elevated porewater nitrate relative to ANME/DSS aggregates in all seep sites examined. This relationship with nitrate was supported by sediment microcosm experiments, in which the abundance of ANME/seepDBB was greater in nitrate-amended incubations relative to the unamended control. FISH-NanoSIMS additionally revealed significantly higher N-15-nitrate incorporation levels in individual aggregates of ANME/seepDBB relative to ANME/DSS aggregates from the same incubation. These combined results suggest that nitrate is a geochemical effector of ANME/seepDBB aggregate distribution, and provides a unique niche for these consortia through their utilization of a greater range of nitrogen substrates than the ANME/DSS.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {1751-7362},
	doi = {10.1038/ismej.2013.147},
	author = {Green-Saxena, A and Dekas, A E and Dalleska, N F and Orphan, V J}
}
@article {Goffredi2004a,
	title = {{Novel forms of structural integration between microbes and a hydrothermal vent gastropod from the Indian Ocean}},
	journal = {Applied and Environmental Microbiology},
	volume = {70},
	number = {5},
	year = {2004},
	pages = {3082{\textendash}3090},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0099-2240},
	doi = {10.1128/AEM.70.5.3082-3090.2004},
	url = {http://dx.doi.org/10.1128/AEM.70.5.3082-3090.2004},
	author = {Goffredi, S K and Waren, A and Orphan, V J and Van Dover, C L and Vrijenhoek, R C}
}
