@article {ISI:000356841900006,
	title = {{Cetia pacifica gen. nov., sp nov., a chemolithoautotrophic, thermophilic, nitrate-ammonifying bacterium from a deep-sea hydrothermal vent}},
	journal = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
	volume = {65},
	number = {4},
	year = {2015},
	month = {apr},
	pages = {1144{\textendash}1150},
	type = {Article},
	abstract = {A thermophilic, anaerobic, chemolithoautotrophic bacterium, strain TB-6(T), was isolated from a deep-sea hydrothermal vent located on the East Pacific Rise at 9 degrees N. The cells were Gram-staining-negative and rod-shaped with one or more polar flagella. Cell size was approximately 1-1.5 mu m in length and 0.5 mu m in width. Strain TB-6(T) grew between 45 and 70 degrees C (optimum 55-60 degrees C), 0 and 35 g NaCl l(-1) (optimum 20-30 gl(-1)) and pH 4.5 and 7.5 (optimum pH 5.5-6.0). Generation time under optimal conditions was 2 h. Growth of strain TB-6(T) occurred with H-2 as the energy source, CO2 as the carbon source and nitrate or sulfur as electron acceptors, with formation of ammonium or hydrogen sulfide, respectively. Acetate, (+)-n-glucose, Casamino acids, sucrose and yeast extract were not used as carbon and energy sources. Inhibition of growth occurred in the presence of lactate, peptone and tryptone under a H-2/CO2 (80 :20; 200 kPa) gas phase. Thiosulfate, sulfite, arsenate, selenate and oxygen were not used as electron acceptors. The G +C content of the genomic DNA was 36.8 molo/o. Phylogenetic analysis of the 16S rRNA gene of strain TB-6(T) showed that this organism branched separately from the three most closely related genera, Caminibacter, Nautilia and Lebetimonas, within the family Nautiliaceae. Strain TB-6(T) contained several unique fatty acids in comparison with other members of the family Nautiliaceae. Based on experimental evidence, it is proposed that the organism represents a novel species and genus within the family Nautiliaceae, Cetia pacifica, gen. nov., sp. nov. The type strain is TB-6(T) (=DSM 27783T=JCM 19563(T)).},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {1466-5026},
	doi = {10.1099/ijs.0.000070},
	author = {Grosche, Ashley and Sekaran, Hema and Perez-Rodriguez, Ileana and Starovoytov, Valentin and Vetriani, Costantino}
}
@article {ISI:000353354100014,
	title = {{From deep-sea volcanoes to human pathogens: a conserved quorum-sensing signal in Epsilonproteobacteria}},
	journal = {ISME Journal},
	volume = {9},
	number = {5},
	year = {2015},
	month = {may},
	pages = {1222{\textendash}1234},
	type = {Article},
	abstract = {Chemosynthetic Epsilonproteobacteria from deep-sea hydrothermal vents colonize substrates exposed to steep thermal and redox gradients. In many bacteria, substrate attachment, biofilm formation, expression of virulence genes and host colonization are partly controlled via a cell density-dependent mechanism involving signal molecules, known as quorum sensing. Within the Epsilonproteobacteria, quorum sensing has been investigated only in human pathogens that use the luxS/autoinducer-2 (AI-2) mechanism to control the expression of some of these functions. In this study we showed that luxS is conserved in Epsilonproteobacteria and that pathogenic and mesophilic members of this class inherited this gene from a thermophilic ancestor. Furthermore, we provide evidence that the luxS gene is expressed-and a quorum-sensing signal is produced-during growth of Sulfurovum lithotrophicum and Caminibacter mediatlanticus, two Epsilonproteobacteria from deep-sea hydrothermal vents. Finally, we detected luxS transcripts in Epsilonproteobacteria-dominated biofilm communities collected from deep-sea hydrothermal vents. Taken together, our findings indicate that the epsiloproteobacterial lineage of the LuxS enzyme originated in high-temperature geothermal environments and that, in vent Epsilonproteobacteria, luxS expression is linked to the production of AI-2 signals, which are likely produced in situ at deep-sea vents. We conclude that the luxS gene is part of the ancestral epsilonproteobacterial genome and represents an evolutionary link that connects thermophiles to human pathogens.},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {1751-7362},
	doi = {10.1038/ismej.2014.214},
	author = {Perez-Rodriguez, Ileana and Bolognini, Marie and Ricci, Jessica and Bini, Elisabetta and Vetriani, Costantino}
}
@article {ISI:000325986500008,
	title = {{Detection and phylogenetic analysis of the membrane-bound nitrate reductase (Nar) in pure cultures and microbial communities from deep-sea hydrothermal vents}},
	journal = {FEMS MICROBIOLOGY ECOLOGY},
	volume = {86},
	number = {2},
	year = {2013},
	month = {nov},
	pages = {256{\textendash}267},
	type = {Article},
	abstract = {Over the past few years the relevance of nitrate respiration in microorganisms from deep-sea hydrothermal vents has become evident. In this study, we surveyed the membrane-bound nitrate reductase (Nar) encoding gene in three different deep-sea vent microbial communities from the East Pacific Rise and the Mid-Atlantic Ridge. Additionally, we tested pure cultures of vent strains for their ability to reduce nitrate and for the presence of the NarG-encoding gene in their genomes. By using the narG gene as a diagnostic marker for nitrate-reducing bacteria, we showed that nitrate reductases related to Gammaproteobacteria of the genus Marinobacter were numerically prevalent in the clone libraries derived from a black smoker and a diffuse flow vent. In contrast, NarG sequences retrieved from a community of filamentous bacteria located about 50cm above a diffuse flow vent revealed the presence of a yet to be identified group of enzymes. 16S rRNA gene-inferred community compositions, in accordance with previous studies, showed a shift from Alpha- and Gammaproteobacteria to Epsilonproteobacteria as the vent fluids become warmer and more reducing. Based on these findings, we argue that Nar-catalyzed nitrate reduction is likely relevant in temperate and less reducing environments where Alpha- and Gammaproteobacteria are more abundant and where nitrate concentrations reflect that of background deep seawater.},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {0168-6496},
	doi = {10.1111/1574-6941.12158},
	author = {Perez-Rodriguez, Ileana and Bohnert, Kenneth A and Cuebas, Mariola and Keddis, Ramaydalis and Vetriani, Costantino}
}
@article {ISI:000311587800012,
	title = {{Phorcysia thermohydrogeniphila gen. nov., sp nov., a thermophilic, chemolithoautotrophic, nitrate-ammonifying bacterium from a deep-sea hydrothermal vent}},
	journal = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
	volume = {62},
	number = {10},
	year = {2012},
	month = {oct},
	pages = {2388{\textendash}2394},
	type = {Article},
	abstract = {A novel hyperthermophilic, anaerobic, chemolithoautotrophic bacterium, designated strain HB-8(T), was isolated from the tube of Alvinella pompejana tubeworms collected from the wall of an actively venting sulfide structure on the East Pacific Rise at 13 degrees N. The cells were Gram-negative rods, approximately 1.0-1.5 mu m long and 0.5 mu m wide. Strain HB-8(T) grew between 65 and 80 degrees C (optimum 75 degrees C), 15 and 35 g NaCl l(-1) (optimum 30 gl(-1)) and pH 4.5 and 8.5 (optimum pH 6.0). Generation time under optimal conditions was 26 min. Growth occurred under chemolithoautotrophic conditions with H-2 as the energy source and CO2 as the carbon source. Nitrate and sulfur were used as electron acceptors, with concomitant formation of ammonium or hydrogen sulfide, respectively. The presence of lactate, formate, acetate or tryptone in the culture medium inhibited growth. The G+C content of the genomic DNA was 47.8 mol{\%}. Phylogenetic analysis of the 16S rRNA gene and of the alpha subunit of the ATP citrate lyase of strain HB-8(T) indicated that this organism formed a novel lineage within the class Aquificae, equally distant from the type strains of the type species of the three genera that represent the family Desulfurobacteriaceae: Thermovibrio ruber ED11/3LLK8(T), Balnearium lithotrophicum 17S(T) and Desulfurobacterium thermolithotrophum BSA(T). The polar lipids of strain HB-8(T) differed substantially from those of other members of the Desulfurobacteriaceae, and this bacterium produced novel quinones. On the basis of phylogenetic, physiological and chemotaxonomic characteristics, it is proposed that the organism represents a novel genus and species within the family Desulfurobacteriaceae, Phorcysia thermohydrogeniphila gen. nov., sp. nov. The type strain of Phorcysia thermohydrogeniphila is HB-8(T) (=DSM 24425(T) =JCM 17384(T)).},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {1466-5026},
	doi = {10.1099/ijs.0.035642-0},
	author = {Perez-Rodriguez, Ileana and Grosche, Ashley and Massenburg, Lynnicia and Starovoytov, Valentin and Lutz, Richard A and Vetriani, Costantino}
}
@article {ISI:000278233400031,
	title = {{Nautilia nitratireducens sp nov., a thermophilic, anaerobic, chemosynthetic, nitrate-ammonifying bacterium isolated from a deep-sea hydrothermal vent}},
	journal = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
	volume = {60},
	number = {5},
	year = {2010},
	month = {may},
	pages = {1182{\textendash}1186},
	type = {Article},
	abstract = {A thermophilic, anaerobic, chemosynthetic bacterium, designated strain MB-1(T), was isolated from the walls of an active deep-sea hydrothermal vent chimney on the East Pacific Rise at 9 degrees 50{\textquoteright} N 10 degrees 17{\textquoteright} W. The cells were Gram-negative-staining rods, approximately 1-1.5 mu m long and 0.3-0.5 mu m wide. Strain MB-1(T) grew at 25-65 degrees C (optimum 55 degrees C), with 10-35 g NaCl l(-1) (optimum 20 g l(-1)) and at pH 4.5-8.5 (optimum pH 7.0). Generation time under optimal conditions was 45.6 min. Growth occurred under chemolithoautotrophic conditions with H(2) as the energy source and CO(2) as the carbon source. Nitrate was used as the electron acceptor, with resulting production of ammonium. Thiosulfate, sulfur and selenate were also used as electron acceptors. No growth was observed in the presence of lactate, peptone or tryptone. Chemo-organotrophic growth occurred in the presence of acetate, formate, Casamino acids, sucrose, galactose and yeast extract under a N(2)/CO(2) gas phase. The G+C content of the genomic DNA was 36.0 mol{\%}. Phylogenetic analysis of the 16S rRNA gene sequence indicated that this organism is closely related to Nautilia profundicola AmH(T), Nautilia abyssi PH1209(T) and Nautilia lithotrophica 525(T) (95, 94 and 93{\%} sequence identity, respectively). On the basis of phylogenetic, physiological and genetic considerations, it is proposed that the organism represents a novel species within the genus Nautilia, Nautilia nitratireducens sp. nov. The type strain is MB-1(T) (=DSM 22087(T) =JCM 15746(T)).},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {1466-5026},
	doi = {10.1099/ijs.0.013904-0},
	author = {Perez-Rodriguez, Ileana and Ricci, Jessica and Voordeckers, James W and Starovoytov, Valentin and Vetriani, Costantino}
}
