@article {ISI:000325986500008,
	title = {{Detection and phylogenetic analysis of the membrane-bound nitrate reductase (Nar) in pure cultures and microbial communities from deep-sea hydrothermal vents}},
	journal = {FEMS MICROBIOLOGY ECOLOGY},
	volume = {86},
	number = {2},
	year = {2013},
	month = {nov},
	pages = {256{\textendash}267},
	type = {Article},
	abstract = {Over the past few years the relevance of nitrate respiration in microorganisms from deep-sea hydrothermal vents has become evident. In this study, we surveyed the membrane-bound nitrate reductase (Nar) encoding gene in three different deep-sea vent microbial communities from the East Pacific Rise and the Mid-Atlantic Ridge. Additionally, we tested pure cultures of vent strains for their ability to reduce nitrate and for the presence of the NarG-encoding gene in their genomes. By using the narG gene as a diagnostic marker for nitrate-reducing bacteria, we showed that nitrate reductases related to Gammaproteobacteria of the genus Marinobacter were numerically prevalent in the clone libraries derived from a black smoker and a diffuse flow vent. In contrast, NarG sequences retrieved from a community of filamentous bacteria located about 50cm above a diffuse flow vent revealed the presence of a yet to be identified group of enzymes. 16S rRNA gene-inferred community compositions, in accordance with previous studies, showed a shift from Alpha- and Gammaproteobacteria to Epsilonproteobacteria as the vent fluids become warmer and more reducing. Based on these findings, we argue that Nar-catalyzed nitrate reduction is likely relevant in temperate and less reducing environments where Alpha- and Gammaproteobacteria are more abundant and where nitrate concentrations reflect that of background deep seawater.},
	keywords = {HOV Alvin (Human Occupied Vehicle)},
	issn = {0168-6496},
	doi = {10.1111/1574-6941.12158},
	author = {Perez-Rodriguez, Ileana and Bohnert, Kenneth A and Cuebas, Mariola and Keddis, Ramaydalis and Vetriani, Costantino}
}
