@article {ISI:000393587200032,
	title = {{Bringing microbial diversity into focus: high-resolution analysis of iron mats from the Lo{\textquoteright}ihi Seamount}},
	journal = {ENVIRONMENTAL MICROBIOLOGY},
	volume = {19},
	number = {1},
	year = {2017},
	month = {jan},
	pages = {301{\textendash}316},
	type = {Article},
	abstract = {Thirty kilometers south of the island of Hawaii lies the Lihi Seamount, an active submarine volcano that hosts a network of low-temperature hydrothermal vents enriched in ferrous iron that supports extensive microbial mats. These mats, which can be a half a meter deep, are composed of ferric iron bound to organic polymers - the metabolic byproduct of iron-oxidizing Zetaproteobacteria. Though the role of Zetaproteobacteria in mat formation is well established, we have a limited understanding of how differences in diversity are related to mat morphology. We used Minimum Entropy Decomposition and ZetaOtu classification to demonstrate cryptic diversity between closely related Zetaproteobacteria while showing habitat and geographic specificity. Veiled mats, common structures at Lihi, exhibit distinct community composition and contain diversity not detected in other mat types, including specific Zetaproteobacteria and an unclassified Gammaproteobacteria. Our analyses also indicate that diversity can change dramatically across small spatial transects from points of active venting, yet we found comparatively few differences between major sampling sites. This study provides a better picture of the microbiome responsible for iron mat production at Lihi and has broad implications for our understanding of these globally distributed communities.},
	keywords = {AUV Sentry (Autonomous Underwater Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {1462-2912},
	doi = {10.1111/1462-2920.13607},
	author = {Scott, Jarrod J and Glazer, Brian T and Emerson, David}
}
@article {ISI:000399660300001,
	title = {{A decade of volcanic construction and destruction at the summit of NW Rota-1 seamount: 2004-2014}},
	journal = {JOURNAL OF GEOPHYSICAL RESEARCH-SOLID EARTH},
	volume = {122},
	number = {3},
	year = {2017},
	month = {mar},
	pages = {1558{\textendash}1584},
	publisher = {AMER GEOPHYSICAL UNION},
	address = {2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA},
	abstract = {Arc volcanoes are important to our understanding of submarine volcanism because at some sites frequent eruptions cause them to grow and collapse on human timescales. This makes it possible to document volcanic processes. Active submarine eruptions have been observed at the summit of NW Rota-1 in the Mariana Arc. We use remotely operated vehicle videography and repeat high-resolution bathymetric surveys to construct geologic maps of the summit of NW Rota-1 in 2009 and 2010 and relate them to the geologic evolution of the summit area over a 10year period (2004-2014). We find that 2009 and 2010 were characterized by different eruptive styles, which affected the type and distribution of eruptive deposits at the summit. Year 2009 was characterized by ultraslow extrusion and autobrecciation of lava at a single eruptive vent, producing a large cone of blocky lava debris. In 2010, higher-energy explosive eruptions occurred at multiple closely spaced vents, producing a thin blanket of pebble-sized tephra overlying lava flow outcrops. A landslide that occurred between 2009 and 2010 had a major effect on lithofacies distribution by removing the debris cone and other unconsolidated deposits, revealing steep massive flow cliffs. This relatively rapid alternation between construction and destruction forms one end of a seamount growth and mass wasting spectrum. Intraplate seamounts, which tend to grow larger than arc volcanoes, experience collapse events that are orders of magnitude larger and much less frequent than those occurring at subduction zone settings. Our results highlight the interrelated cyclicity of eruptive activity and mass wasting at submarine arc volcanoes.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {2169-9313},
	doi = {10.1002/2016JB013742},
	author = {Schnur, Susan R and Chadwick Jr., William W and Embley, Robert W and Ferrini, Vicki L and de Ronde, Cornel E J and Cashman, Katharine V and Deardorff, Nicholas D and Merle, Susan G and Dziak, Robert P and Haxel, Joe H and Matsumoto, Haru}
}
@article {Ross2017,
	title = {{Deep-sea coral and hardbottom habitats on the west Florida slope, eastern Gulf of Mexico}},
	journal = {Deep Sea Research Part I: Oceanographic Research Papers},
	volume = {120},
	year = {2017},
	pages = {14{\textendash}28},
	abstract = {Until recently, benthic habitats dominated by deep-sea corals (DSC) appeared to be less extensive on the slope of the Gulf of Mexico (GOM) than in the northeast Atlantic Ocean or off the southeastern US. There are relatively few bioherms (i.e., coral-built mounds) in the northern GOM, and most DSCs are attached to existing hard substrata (e.g., authigenically formed carbonate). The primary structure-forming, DSC in the GOM is Lophelia pertusa, but structure is also provided by other living and dead scleractinians, antipatharians (black corals), octocorals (gorgonians, soft corals), hydrocorals and sponges, as well as abundant rocky substrata. The best development of DSCs in the GOM was previously documented within Viosca Knoll oil and gas lease blocks 826 and 862/906 (north-central GOM) and on the Campeche Bank (southern GOM in Mexican waters). This paper documents extensive deep reef ecosystems composed of DSC and rocky hard-bottom recently surveyed on the West Florida Slope (WFS, eastern GOM) during six research cruises (2008{\textendash}2012). Using multibeam sonar, CTD casts, and video from underwater vehicles, we describe the physical and oceanographic characteristics of these deep reefs and provide size or area estimates of deep coral and hardground habitats. The multibeam sonar analyses revealed hundreds of mounds and ridges, some of which were subsequently surveyed using underwater vehicles. Mounds and ridges in {\textless}525 m depths were usually capped with living coral colonies, dominated by L. pertusa. An extensive rocky scarp, running roughly north-south for at least 229 km, supported lower abundances of scleractinian corals than the mounds and ridges, despite an abundance of settlement substrata. Areal comparisons suggested that the WFS may exceed other parts of the GOM slope in extent of living deep coral coverage and other deep-reef habitat (dead coral and rock). The complex WFS region warrants additional studies to better understand the influences of oceanography and geology on the occurrence of DSC and associated organisms. Protection measures are being considered to ensure the long-term integrity of this diverse ecosystem.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.dsr.2016.12.005},
	author = {Ross, S W and Rhode, M and Brooke, S}
}
@article {Brooke2017,
	title = {{Distributions and habitat associations of deep-water corals in Norfolk and Baltimore Canyons, Mid-Atlantic Bight, USA}},
	journal = {Deep Sea Research Part II: Topical Studies in Oceanography},
	volume = {137},
	year = {2017},
	pages = {131{\textendash}147},
	abstract = {A multi-disciplinary study of two major submarine canyons, Baltimore Canyon and Norfolk Canyon, off the US mid-Atlantic coast focused on the ecology and biology of canyon habitats, particularly those supporting deep-sea corals. Historical data on deep-sea corals from these canyons were sparse with less than 750 records for the mid-Atlantic region, with most being soft sediment species. This study substantially increased the number of deep-sea coral records for the target canyons and the region. Large gorgonians were the dominant structure-forming coral taxa on exposed hard substrates, but several species of scleractinians were also documented, including first observations of Lophelia pertusa in the mid-Atlantic Bight region. Coral distribution varied within and between the two canyons, with greater abundance of the octocoral Paragorgia arborea in Baltimore Canyon, and higher occurrence of stony corals in Norfolk Canyon; these observations reflect the differences in environmental conditions, particularly turbidity, between the canyons. Some species have a wide distribution (e.g., P. arborea, Primnoa resedaeformis, Anthothela grandiflora), while others are limited to certain habitat types and/or depth zones (e.g., Paramuricea placomus, L. pertusa, Solenosmilia variabilis). The distribution of a species is driven by a combination of factors, which include availability of appropriate physical structure and environmental conditions. Although the diversity of the structure-forming corals (gorgonians, branching scleractinians and large anemones) was low, many areas of both canyons supported high coral abundance and a diverse coral-associated community. The canyons provide suitable habitat for the development of deep-sea coral communities that is not readily available elsewhere on the sedimented shelf and slope of the Mid-Atlantic Bight.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.dsr2.2016.05.008},
	author = {Brooke, S D and Watts, M W and Heil, A D and Rhode, M and Mienis, F and Duineveld, G C A and Davies, A J and Ross, S W}
}
@article {Sylvan2017,
	title = {{Evidence for microbial mediation of subseafloor nitrogen redox processes at Loihi Seamount, Hawaii}},
	journal = {Geochimica et Cosmochimica Acta},
	volume = {198},
	year = {2017},
	pages = {131{\textendash}150},
	abstract = {The role of nitrogen cycling in submarine hydrothermal systems is far less studied than that of other biologically reactive elements such as sulfur and iron. In order to address this knowledge gap, we investigated nitrogen redox processes at Loihi Seamount, Hawaii, using a combination of biogeochemical and isotopic measurements, bioenergetic calculations and analysis of the prokaryotic community composition in venting fluids sampled during four cruises in 2006, 2008, 2009 and 2013. Concentrations of NH4+ were positively correlated to dissolved Si and negatively correlated to NO3- + NO2-, while NO2- was not correlated to NO3- + NO2-, dissolved Si or NH4+. This is indicative of hydrothermal input of NH4+ and biological mediation influencing NO2- concentrations. The stable isotope ratios of NO3- ($δ$15N and $δ$18O) was elevated with respect to background seawater, with $δ$18O values exhibiting larger changes than corresponding $δ$15N values, reflecting the occurrence of both production and reduction of NO3- by an active microbial community. $δ$15N-NH4+ values ranged from 0{\textperthousand} to +16.7{\textperthousand}, suggesting fractionation during consumption and potentially N-fixation as well. Bioenergetic calculations reveal that several catabolic strategies involving the reduction of NO3- and NO2- coupled to sulfide and iron oxidation could provide energy to microbes in Loihi fluids, while 16S rRNA gene sequencing of Archaea and Bacteria in the fluids reveals groups known to participate in denitrification and N-fixation. Taken together, our data support the hypothesis that microbes are mediating N-based redox processes in venting hydrothermal fluids at Loihi Seamount.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.gca.2016.10.029},
	author = {Sylvan, Jason B. and Wankel, Scott D. and LaRowe, Douglas E. and Charoenpong, Chawalit N. and Huber, Julie A. and Moyer, Craig L. and Edwards, Katrina J.}
}
@article {LaBella2017,
	title = {{Gene flow between Atlantic and Pacific Ocean basins in three lineages of deep-sea clams (Bivalvia: Vesicomyidae: Pliocardiinae) and subsequent limited gene flow within the Atlantic}},
	journal = {Deep Sea Research Part II: Topical Studies in Oceanography},
	volume = {137},
	year = {2017},
	pages = {307{\textendash}317},
	abstract = {Pliocardiin (vesicomyid) clams rely on microbial symbionts for nutrition and are obligate inhabitants of deep-sea chemosynthetic ecosystems. Unlike many other invertebrate hosts of chemosynthetic microbes, pliocardiin clams are found in every ocean in a variety of reducing habitats, including hydrothermal vents, cold seeps, organic falls and deep-sea fans. The global distribution of pliocardiin clams suggests historical gene flow between ocean basins. We focus on 3 pliocardiin genera{\textemdash}{\textquoteleft}Pliocardia{\textquoteright} I, Calyptogena and Abyssogena{\textemdash}each of which has a pair of sister clades in the Atlantic and Pacific. Our work tests the hypothesis that historical gene flow between the Atlantic and Pacific Oceans within these genera was interrupted by the closure of the Panamanian seaway and tests whether isolation between the ocean basins is the result of vicariance or past colonization. These questions are investigated in the context of fossil evidence, biogeography and phylogenetics. This study revealed a set of substitution rates consistent with other invertebrate studies ($μ$=0.8{\%}/My/lineage), and a set consistent with much lower rates often attributed to deep-sea organisms ($μ$=0.3{\%}/My/lineage). Among the Pacific/Atlantic sister pairs, {\textquoteleft}Pliocardia{\textquoteright} I COI divergence per lineage is intermediate (2.5{\%}), Calyptogena is the highest (6.1{\%}) and Abyssogena the lowest (0.8{\%}). The substitution rates suggest that {\textquoteleft}Pliocardia{\textquoteright} I and Calyptogena have histories of at least 2.8 My in the Atlantic, with Calyptogena likely older. The slower rate, however, is inconsistent with both the maximum age of the family and several well studied fossils: leaving the faster rate preferred. With the faster rate, the Abyssogena southwardae clade diverged from its Pacific sister clade around 1 Mya, which likely post-dates the closure of the Isthmus of Panama and the opening of the Bering Strait. In light of this recent divergence, we test the previously proposed hypothesis that there is a high level of ongoing gene flow between Atlantic populations of A. southwardae. A. southwardae has colonized a broad geographic range of seep sites including the West Florida Escarpment, the Barbados Accretionary Prism, the Lobes of Congo, and the Mid-Atlantic Ridge north and south of the Romanche Transform Fault. Coalescent methods detect gene flow between Barbados and the Mid-Atlantic ridge; and between the West Florida Escarpment and the Lobes of Congo. All other comparisons failed to detect gene flow, contrary to prevailing interpretations of connectivity across the entire Atlantic Basin.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.dsr2.2016.08.013},
	author = {LaBella, A L and Van Dover, C L and Jollivet, D and Cunningham, C W}
}
@article {Bourque2017,
	title = {{Macrofaunal communities associated with chemosynthetic habitats from the U.S. Atlantic margin: A comparison among depth and habitat types}},
	journal = {Deep Sea Research Part II: Topical Studies in Oceanography},
	volume = {137},
	year = {2017},
	pages = {42{\textendash}55},
	abstract = {Hydrocarbon seeps support distinct benthic communities capable of tolerating extreme environmental conditions and utilizing reduced chemical compounds for nutrition. In recent years, several locations of methane seepage have been mapped along the U.S. Atlantic continental slope. In 2012 and 2013, two newly discovered seeps were investigated in this region: a shallow site near Baltimore Canyon (BCS, 366{\textendash}412 m) and a deep site near Norfolk Canyon (NCS, 1467{\textendash}1602 m), with both sites containing extensive chemosynthetic mussel bed and microbial mat habitats. Sediment push cores, suction samples, and Ekman box cores were collected to quantify the abundance, diversity, and community structure of benthic macrofauna ({\textgreater}300 $μ$m) in mussel beds, mats, and slope habitats at both sites. Community data from the deep site were also assessed in relation to the associated sediment environment (organic carbon and nitrogen, stable carbon and nitrogen isotopes, grain size, and depth). Infaunal assemblages and densities differed both between depths and among habitat types. Macrofaunal densities in microbial mats were four times greater than those present in mussel beds and slope sediments and were dominated by the annelid families Dorvilleidae, Capitellidae, and Tubificidae, while mussel habitats had higher proportions of crustaceans. Diversity was lower in BCS microbial mat habitats, but higher in mussel and slope sediments compared to NCS habitats. Multivariate statistical analysis revealed specific sediment properties as important for distinguishing the macrofaunal communities, including larger grain sizes present within NCS microbial mat habitats and depleted stable carbon isotopes ($δ$13C) in sediments present at mussel beds. These results suggest that habitat differences in the quality and source of organic matter are driving the observed patterns in the infaunal assemblages, including high $\beta$ diversity and high variability in the macrofaunal community composition. This study is the first investigation of seep infauna along the U.S. Atlantic slope north of the Blake Ridge Diapir and provides a baseline for future regional comparisons to other seep habitats along the Atlantic margin.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.dsr2.2016.04.012},
	author = {Bourque, J R and Robertson, C M and Brooke, S and Demopoulos, A W J}
}
@article {Levin2017,
	title = {{Methane seepage effects on biodiversity and biological traits of macrofauna inhabiting authigenic carbonates}},
	journal = {Deep Sea Research Part II: Topical Studies in Oceanography},
	volume = {137},
	year = {2017},
	pages = {26{\textendash}41},
	abstract = {Authigenic carbonate rocks at methane seeps are recognized as hosting diverse and abundant invertebrate assemblages, with potential forcing from fluid seepage and hydrography. Mensurative studies of carbonate macrofauna ({\textgreater}0.3 mm) at Hydrate Ridge, OR revealed little effect of water depth and overlying oxygenation (at 600 m and 800 m) but a large influence of seepage activity on density, taxonomic composition, diversity, and biological traits (feeding, lifestyle, motility, size and calcification). Rocks exposed to active seepage had 3{\textendash}4{\texttimes} higher total macrofaunal densities than under inactive conditions. Assemblages exhibited higher species richness and reduced evenness (greater dominance) under active seepage than inactive conditions, but no difference in H' or rarefaction diversity. Actively seeping sites were characterized by errant (motile), bacterial grazing, small- and medium-sized, heavily calcified species, whereas inactive sites exhibited a greater diversity of feeding modes and more burrowers, sessile, large and lightly calcified species. Active rocks supported more exogonid (Syllidae), ampharetid, and cirratulid polychaetes, provannid snails, pyropeltid limpets, nemerteans, and sponges; whereas inactive rocks supported higher densities of ophiuroids, isopods, gammarid amphipods, hydroids, Typosyllis (Syllidae) and tanaids. Transplant experiments, in which rocks were transferred between active and inactive sites at Hydrate Ridge North (600 m), revealed that assemblages respond within 13 months to increase or cessation of seepage, taking on the feeding, size and calcification characteristics of the background fauna at the new site. Lifestyles and motility patterns shifted more slowly as the sessile, attached species did not track seepage as quickly. Provannid snails and pyropeltid limpets rapidly colonized rocks transplanted to active sites and disappeared when transplanted to inactive sites. Given the known variability of fluid fluxes and rapid community response, a mosaic of communities changing in space and time is hypothesized to generate the relatively high species diversity at methane seeps.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.dsr2.2016.05.021},
	author = {Levin, L A and Mendoza, G F and Grupe, B M}
}
@article {McNichol2016a,
	title = {{Assessing microbial processes in deep-sea hydrothermal systems by incubation at in situ temperature and pressure}},
	journal = {Deep Sea Research Part I: Oceanographic Research Papers},
	volume = {115},
	year = {2016},
	pages = {221{\textendash}232},
	abstract = {At deep-sea hydrothermal vents, a large source of potential chemical energy is created when reducing vent fluid and oxidizing seawater mix. In this environment, chemolithoautotrophic microbes catalyze exergonic redox reactions which in turn provide the energy needed to fuel their growth and the fixation of CO2 into biomass. In addition to producing new organic matter, this process also consumes compounds contained both in vent fluid and entrained seawater (e.g. H2, NO3-). Despite their biogeochemical importance, such reactions have remained difficult to quantify due to methodological limitations. To address this knowledge gap, this study reports a novel application of isobaric gas-tight fluid samplers for conducting incubations of hydrothermal vent fluids at in situ temperature and pressure. Eighteen {\~{}}24 h incubations were carried out, representing seven distinct conditions that examine amendments consisting of different electron donors and acceptors. Microbial activity was observed in all treatments, and time series chemical measurements showed that activity was limited by electron acceptor supply, confirming predictions based on geochemical data. Also consistent with these predictions, the presence of nitrate increased rates of hydrogen consumption and yielded ammonium as a product of nitrate respiration. The stoichiometry of predicted redox reactions was also determined, revealing that the sulfur and nitrogen cycles are incompletely understood at deep-sea vents, and likely involve unknown intermediate redox species. Finally, the measured rates of redox processes were either equal to or far greater than what has been reported in previous studies where in situ conditions were not maintained. In addition to providing insights into deep-sea hydrothermal vent biogeochemistry, the methods described herein also offer a practical approach for the incubation of any deep-sea pelagic sample under in situ conditions.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.dsr.2016.06.011},
	author = {McNichol, J and Sylva, S P and Thomas, F and Taylor, C D and Sievert, S M and Seewald, J S}
}
@article {Burkett2016,
	title = {{Colonization of over a thousand Cibicidoides wuellerstorfi (foraminifera: Schwager, 1866) on artificial substrates in seep and adjacent off-seep locations in dysoxic, deep-sea environments}},
	journal = {Deep Sea Research Part I: Oceanographic Research Papers},
	volume = {117},
	year = {2016},
	pages = {39{\textendash}50},
	abstract = {After {\~{}}1 yr on the seafloor at water depths of {\~{}}700 m on Hydrate Ridge in the Pacific, eight colonization experiments composed primarily of a plastic mesh cube (from here on refered to as SEA3, for Seafloor Epibenthic Attachment Cubes) were colonized by 1076 Cibicidoides wuellerstorfi on {\~{}}1841 cm2 of experimental substrate. This species is typically considered an indicator of well-oxygenated conditions, and recruitment of such large numbers in bottom waters with low dissolved oxygen availability (0.24{\textendash}0.37 mL/L) indicate that this taxon may not be as limited by oxygen as previously thought. Clues about substrate preferences were evident from the distribution, or lack thereof, of individuals among plastic mesh, coated steel frame, wooden dowels and reflective tape. Abundance, individual size distributions within cage populations and isotopic biogeochemistry of living foraminifera colonizing experimental substrates were compared between active seep and adjacent off-seep experiment locations, revealing potential differences between these environments. Few studies have examined foraminiferal colonization of hard substrates in the deep-sea and to our knowledge no previous study has compared foraminiferal colonization of active seep and off-seep substrates from the same region. This study provides initial results of recruitment, colonization, geochemical and morphological aspects of the paleoceanographically significant species, C. wuellerstorfi, from dynamic deep-sea environments. Further experimental deployments of SEA3s will provide a means to assess relatively unknown ecologic dynamics of important foraminiferal deep-sea species.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.dsr.2016.08.011},
	author = {Burkett, A M and Rathburn, A E and Perez, Elena M and Levin, L A and Martin, J B}
}
@article {Gueguen2016,
	title = {{Comparative geochemistry of four ferromanganese crusts from the Pacific Ocean and significance for the use of Ni isotopes as paleoceanographic tracers}},
	journal = {Geochimica et Cosmochimica Acta},
	volume = {189},
	year = {2016},
	pages = {214{\textendash}235},
	abstract = {{Ferromanganese (Fe-Mn) crusts are potential archive of the Ni isotope composition of seawater through time. In this study we aim at (1) understanding Ni isotope fractionation mechanisms and metal enrichment processes in Fe-Mn deposits, (2) addressing global vs. local control of Ni isotope composition of these deposits. Two Fe-Mn crusts from the North Pacific Ocean (Apuupuu Seamount, Hawaii) and two Fe-Mn crusts from the South Pacific Ocean (near Rurutu Island, Austral archipelago of French Polynesia) were characterized for their elemental geochemistry and Ni isotope composition. Geochemical analyses were performed at millimeter intervals in order to provide time-resolved record of Ni isotopes. Chronology and growth rates were determined using cosmogenic 10Be isotope abundances. The results show that, despite different growth rates, textures and geochemical patterns, Fe-Mn crusts from both North and South Pacific Oceans have fairly homogenous Ni isotope compositions over the last \~{}17 Ma, yielding average $δ$60/58Ni values of 1.79 {\textpm} 0.21{\textperthousand} (2sd},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.gca.2016.06.005},
	author = {Gueguen, B and Rouxel, O J and Rouget, M-L and Bollinger, C and Ponzevera, E and Germain, Y and Fouquet, Y}
}
@article {Thresher2016,
	title = {{A {\textquotedblleft}core-top{\textquotedblright} screen for trace element proxies of environmental conditions and growth rates in the calcite skeletons of bamboo corals (Isididae)}},
	journal = {Geochimica et Cosmochimica Acta},
	volume = {193},
	year = {2016},
	pages = {75{\textendash}99},
	abstract = {We test for trace element proxies in the high-magnesium calcite fraction of bamboo coral internodes by comparing environmental conditions and growth rates to the specimen-mean compositions of 73 corals that were live-caught at depths ranging from 3 to 3950 m and collected from habitats ranging from tropical coral reefs to the Antarctic slope. Comparisons were done at a large geographic scale (LGS) and for a well sampled area south of Australia, across depths at a single site, in order to help separate the effects of environmental variables that co-vary at one spatial scale, but not the other. Thirty-seven trace elements were measured using solution-based Sector Field ICP-MS, of which seventeen were significantly detected in more than a third of the specimens. Only eight element/calcium ratios correlated significantly with any environmental variable at the large geographic scale, and only four did so at the local level. At the LGS, the highest correlation was between ambient temperature and Mg/Ca, which accounted for 89{\%} of the variance across specimens, spanned all four Isidid sub-families and was independently significant in the two best sampled sub-families. The predictive (geometric mean) relationship is View the MathML sourceT({\textdegree}C)=-23.9({\textpm}2.46)+0.34({\textpm}0.25)Mg/Ca(mmol/mol) Turn MathJax on spanning a temperature range of -1.9 to 26.8 {\textdegree}C, Mg/Ca ratios from 58.6 to 155.1 mmol/mol, and an uncertainty (RMS) of 2.78 {\textdegree}C. The numbers in parentheses are 95{\%} CIs. The slope of the regression does not differ significantly from that of abiotic high-Mg calcites, which suggests that the temperature-dependent incorporation of Mg into the carbonate results from kinetic reactions at the crystal surface. Analysis at the SH scale for the sub-set of specimens for which we had data suggests is also affected by growth rates. There were no obvious trace element correlates at either spatial scale of salinity or oxygen levels that could not be accounted for by covariance between these environmental parameters and, in most cases, temperature. Single and multiple correlation analyses also confirm previous suggestions that Ba/Ca in bamboo coral calcite is a proxy for seawater barium and hence refractory nutrients, suggest that Sr/Ca is influenced by specimen-mean Mg/Ca ratios and water temperature as well as possibly seawater Sr/Ca, and falsify for bamboo corals P/Ca (as well as P/Cd and Cd/Ca) as a proxy for seawater phosphate levels. The predictive relationship between Isidid skeletal-mean Ba/Ca and seawater silicate concentrations appears to be linear, and is given by View the MathML sourcesilicate($μ$molkg-1)=-56.7({\textpm}20.8)+9217({\textpm}1632)Ba/Ca(mmol/mol) Turn MathJax on spanning a silicate range of 0.5 to 120 $μ$mol kg-1, a Ba/Ca range of 0.0042 to 0.0195 mmol/mol, and with an uncertainty (RMS) of 33.1 $μ$mol kg-1. Mn/Ca differences among specimens and sites are highly significant and appear to reflect seawater Mn, suggesting a proxy for this micronutrient. The compilation of growth rate data across 34 specimens indicates a wide range of growth rates even among con-familial specimens from within a single habitat, and suggests both ambient temperature and food availability underlie at least part of this variability.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.gca.2016.07.033},
	author = {Thresher, R E and Fallon, S J and Townsend, A T}
}
@article {Neira2016,
	title = {{Cross-hole tracer experiment reveals rapid fluid flow and low effective porosity in the upper oceanic crust}},
	journal = {Earth and Planetary Science Letters},
	volume = {450},
	year = {2016},
	pages = {355{\textendash}365},
	abstract = {Numerous field, laboratory, and modeling studies have explored the flows of fluid, heat, and solutes during seafloor hydrothermal circulation, but it has been challenging to determine transport rates and flow directions within natural systems. Here we present results from the first cross-hole tracer experiment in the upper oceanic crust, using four subseafloor borehole observatories equipped with autonomous samplers to track the transport of a dissolved tracer (sulfur hexafluoride, SF6) injected into a ridge-flank hydrothermal system. During the first three years after tracer injection, SF6 was transported both north and south through the basaltic aquifer. The observed tracer transport rate of \~{}2{\textendash}3 m/day is orders of magnitude greater than bulk rates of flow inferred from thermal and chemical observations and calculated with coupled fluid-heat flow simulations. Taken together, these results suggest that the effective porosity of the upper volcanic crust through which much tracer was transported is {\textless}1{\%}, with fluid flowing rapidly along a few well-connected channels. This is consistent with the heterogeneous (layered, faulted, and/or fractured) nature of the volcanic upper oceanic crust.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.epsl.2016.06.048},
	author = {Neira, N M and Clark, J F and Fisher, A T and Wheat, C G and Haymon, R M and Becker, K}
}
@article {ISI:000371206300001,
	title = {{A distinct and active bacterial community in cold oxygenated fluids circulating beneath the western flank of the Mid-Atlantic ridge}},
	journal = {SCIENTIFIC REPORTS},
	volume = {6},
	year = {2016},
	month = {mar},
	type = {Article},
	abstract = {The rock-hosted, oceanic crustal aquifer is one of the largest ecosystems on Earth, yet little is known about its indigenous microorganisms. Here we provide the first phylogenetic and functional description of an active microbial community residing in the cold oxic crustal aquifer. Using subseafloor observatories, we recovered crustal fluids and found that the geochemical composition is similar to bottom seawater, as are cell abundances. However, based on relative abundances and functional potential of key bacterial groups, the crustal fluid microbial community is heterogeneous and markedly distinct from seawater. Potential rates of autotrophy and heterotrophy in the crust exceeded those of seawater, especially at elevated temperatures (25 degrees C) and deeper in the crust. Together, these results reveal an active, distinct, and diverse bacterial community engaged in both heterotrophy and autotrophy in the oxygenated crustal aquifer, providing key insight into the role of microbial communities in the ubiquitous cold dark subseafloor biosphere.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {2045-2322},
	doi = {10.1038/srep22541},
	author = {Meyer, Julie L and Jaekel, Ulrike and Tully, Benjamin J and Glazer, Brian T and Wheat, C Geoffrey and Lin, Huei-Ting and Hsieh, Chih-Chiang and Cowen, James P and Hulme, Samuel M and Girguis, Peter R and Huber, Julie A}
}
@article {Edgcomb2016,
	title = {{Gene expression profiling of microbial activities and interactions in sediments under haloclines of E. Mediterranean deep hypersaline anoxic basins}},
	journal = {ISME Journal},
	volume = {10},
	year = {2016},
	pages = {2643{\textendash}2657},
	abstract = {Deep-sea hypersaline anoxic basins (DHABs) in the Eastern Mediterranean Sea are considered some of the most polyextreme habitats on Earth. In comparison to microbial activities occurring within the haloclines and brines of these unusual water column habitats near the Mediterranean seafloor, relatively little is known about microbial metabolic activities in the underlying sediments. In addition, it is not known whether activities are shaped by the unique chemistries of the different DHAB brines and whether evidence exists for active microbial eukaryotes in those sediments. Metatranscriptome analysis was applied to sediment samples collected using ROV Jason from underneath the haloclines of Urania, Discovery and L{\textquoteright}Atalante DHABs and a control site. We report on expression of genes associated with sulfur and nitrogen cycling, putative osmolyte biosynthetic pathways and ion transporters, trace metal detoxification, selected eukaryotic activities (particularly of fungi), microbemicrobe interactions, and motility in sediments underlying the haloclines of three DHABs. Relative to our control sediment sample collected outside of Urania Basin, microbial communities (including eukaryotes) in the Urania and Discovery DHAB sediments showed upregulation of expressed genes associated with nitrogen transformations, osmolyte biosynthesis, heavy metals resistance and metabolism, eukaryotic organelle functions, and cell-cell interactions. Sediments underlying DHAB haloclines that have cumulative physico-chemical stressors within the limits of tolerance for microoorganisms can therefore be hotspots of activity in the deep Mediterranean Sea.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1038/ismej.2016.58},
	author = {Edgcomb, V P and Pachiadaki, M and Mara, P and Kormas, K and Leadbetter, E R and Bernhard, J M}
}
@article {Prouty2016a,
	title = {{Growth rates and ages of deep-sea corals impacted by the Deepwater Horizon oil spill}},
	journal = {Deep Sea Research Part II: Topical Studies in Oceanography},
	volume = {129},
	year = {2016},
	pages = {196{\textendash}212},
	abstract = {The impact of the April 2010 Deepwater Horizon (DWH) spill on deep-sea coral communities in the Gulf of Mexico (GoM) is still under investigation, as is the potential for these communities to recover. Impacts from the spill include observation of corals covered with flocculent material, with bare skeleton, excessive mucous production, sloughing tissue, and subsequent colonization of damaged areas by hydrozoans. Information on growth rates and life spans of deep-sea corals is important for understanding the vulnerability of these ecosystems to both natural and anthropogenic perturbations, as well as the likely duration of any observed adverse impacts. We report radiocarbon ages and radial and linear growth rates based on octocorals (Paramuricea spp. and Chrysogorgia sp.) collected in 2010 and 2011 from areas of the DWH impact. The oldest coral radiocarbon ages were measured on specimens collected 11 km to the SW of the oil spill from the Mississippi Canyon (MC) 344 site: 599 and 55 cal yr BP, suggesting continuous life spans of over 600 years for Paramuricea biscaya, the dominant coral species in the region. Calculated radial growth rates, between 0.34 $μ$m yr-1 and 14.20 $μ$m yr-1, are consistent with previously reported proteinaceous corals from the GoM. Anomalously low radiocarbon ($Δ$14C) values for soft tissue from some corals indicate that these corals were feeding on particulate organic carbon derived from an admixture of modern surface carbon and a low 14C carbon source. Results from this work indicate fossil carbon could contribute 5{\textendash}10{\%} to the coral soft tissue $Δ$14C signal within the area of the spill impact. The influence of a low 14C carbon source (e.g., petro-carbon) on the particulate organic carbon pool was observed at all sites within 30 km of the spill site, with the exception of MC118, which may have been outside of the dominant northeast{\textendash}southwest zone of impact. The quantitatively assessed extreme longevity and slow growth rates documented here highlight the vulnerability of these long-lived deep sea coral species to disturbance.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.dsr2.2014.10.021},
	author = {Prouty, N G and Fisher, C R and Demopoulos, A W J and Druffel, E R M}
}
@article {ISI:000372931000004,
	title = {{Hydrogen and thiosulfate limits for growth of a thermophilic, autotrophic Desulfurobacterium species from a deep-sea hydrothermal vent}},
	journal = {ENVIRONMENTAL MICROBIOLOGY REPORTS},
	volume = {8},
	number = {2},
	year = {2016},
	month = {apr},
	pages = {196{\textendash}200},
	type = {Article},
	abstract = {Hydrothermal fluids (341 degrees C and 19 degrees C) were collected {\textless}1m apart from a black smoker chimney and a tubeworm mound on the Boardwalk edifice at the Endeavour Segment in the northeastern Pacific Ocean to study anaerobic microbial growth in hydrothermal mineral deposits. Geochemical modelling of mixed vent fluid and seawater suggests the mixture was anoxic above 55 degrees C and that low H-2 concentrations (79molkg(-1) in end-member hydrothermal fluid) limit anaerobic hydrogenotrophic growth above this temperature. A thermophilic, hydrogenotrophic sulfur reducer, Desulfurobacterium strain HR11, was isolated from the 19 degrees C fluid raising questions about its H-2-dependent growth kinetics. Strain HR11 grew at 40-77 degrees C (T-opt 72-75 degrees C), pH 5-8.5 (pH(opt) 6-7) and 1-5{\%} (wt vol(-1)) NaCl (NaClopt 3-4{\%}). The highest growth rates occurred when S2O32- and S degrees were reduced to H2S. Modest growth occurred by NO3- reduction. Monod constants for its growth were K-s of 30M for H-2 and K-s of 20M for S2O32- with a (max) of 2.0h(-1). The minimum H-2 and S2O32- concentrations for growth were 3M and 5M respectively. Possible sources of S2O32- and S degrees are from abiotic dissolved sulfide and pyrite oxidation by O-2.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1758-2229},
	doi = {10.1111/1758-2229.12368},
	author = {Stewart, Lucy C and Llewellyn, James G and Butterfield, David A and Lilley, Marvin D and Holden, James F}
}
@article {ISI:000390261300037,
	title = {{Inflation-predictable behavior and co-eruption deformation at Axial Seamount}},
	journal = {SCIENCE},
	volume = {354},
	number = {6318},
	year = {2016},
	month = {dec},
	pages = {1399{\textendash}1403},
	type = {Article},
	abstract = {Deformation of the ground surface at active volcanoes provides information about magma movements at depth. Improved seafloor deformation measurements between 2011 and 2015 documented a fourfold increase in magma supply and confirmed that Axial Seamount{\textquoteright}s eruptive behavior is inflation-predictable, probably triggered by a critical level of magmatic pressure. A 2015 eruption was successfully forecast on the basis of this deformation pattern and marked the first time that deflation and tilt were captured in real time by a new seafloor cabled observatory, revealing the timing, location, and volume of eruption-related magma movements. Improved modeling of the deformation suggests a steeply dipping prolate-spheroid pressure source beneath the eastern caldera that is consistent with the location of the zone of highest melt within the subcaldera magma reservoir determined from multichannel seismic results.},
	keywords = {AUV Sentry (Autonomous Underwater Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0036-8075},
	doi = {10.1126/science.aah4666},
	author = {Nooner, Scott L and Chadwick Jr., William W}
}
@article {Prouty2016,
	title = {{Insights into methane dynamics from analysis of authigenic carbonates and chemosynthetic mussels at newly-discovered Atlantic Margin seeps}},
	journal = {Earth and Planetary Science Letters},
	volume = {449},
	year = {2016},
	pages = {332{\textendash}344},
	abstract = {The recent discovery of active methane venting along the US northern and mid-Atlantic margin represents a new source of global methane not previously accounted for in carbon budgets from this region. However, uncertainty remains as to the origin and history of methane seepage along this tectonically inactive passive margin. Here we present the first isotopic analyses of authigenic carbonates and methanotrophic deep-sea mussels, Bathymodiolus sp., and the first direct constraints on the timing of past methane emission, based on samples collected at the upper slope Baltimore Canyon (\~{}385 m water depth) and deepwater Norfolk (\~{}1600 m) seep fields within the area of newly-discovered venting. The authigenic carbonates at both sites were dominated by aragonite, with an average View the MathML source$δ$C13 signature of -47{\textperthousand}-47{\textperthousand}, a value consistent with microbially driven anaerobic oxidation of methane-rich fluids occurring at or near the sediment{\textendash}water interface. Authigenic carbonate U and Sr isotope data further support the inference of carbonate precipitation from seawater-derived fluids rather than from formation fluids from deep aquifers. Carbonate stable and radiocarbon (View the MathML source$δ$C13 and View the MathML source$Δ$C13) isotope values from living Bathymodiolus sp. specimens are lighter than those of seawater dissolved inorganic carbon, highlighting the influence of fossil carbon from methane on carbonate precipitation. U{\textendash}Th dates on authigenic carbonates suggest seepage at Baltimore Canyon between 14.7{\textpm}0.6 ka14.7{\textpm}0.6 ka to 15.7{\textpm}1.6 ka15.7{\textpm}1.6 ka, and at the Norfolk seep field between 1.0{\textpm}0.7 ka1.0{\textpm}0.7 ka to 3.3{\textpm}1.3 ka3.3{\textpm}1.3 ka, providing constraint on the longevity of methane efflux at these sites. The age of the brecciated authigenic carbonates and the occurrence of pockmarks at the Baltimore Canyon upper slope could suggest a link between sediment delivery during Pleistocene sea-level lowstand, accumulation of pore fluid overpressure from sediment compaction, and release of overpressure through subsequent venting. Calculations show that the Baltimore Canyon site probably has not been within the gas hydrate stability zone (GHSZ) in the past 20 ka, meaning that in-situ release of methane from dissociating gas hydrate cannot be sustaining the seep. We cannot rule out updip migration of methane from dissociation of gas hydrate that occurs farther down the slope as a source of the venting at Baltimore Canyon, but consider that the history of rapid sediment accumulation and overpressure may play a more important role in methane emissions at this site.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.epsl.2016.05.023},
	author = {Prouty, N G and Sahy, D and Ruppel, C D and Roark, E B and Condon, D and Brooke, S and Ross, S W and Demopoulos, A W J}
}
@article {Macelloni2016,
	title = {{Mesoscale biogeophysical characterization of Woolsey Mound (northern Gulf of Mexico), a new attribute of natural marine hydrocarbon seeps architecture}},
	journal = {Marine Geology},
	volume = {380},
	year = {2016},
	pages = {330{\textendash}344},
	abstract = {Located on the continental slope in 900 m of water, Woolsey Mound dominates seafloor morphology at Mississippi Canyon 118. The carbonate-hydrate mound is the site of the Gulf of Mexico Hydrates Research Consortium{\textquoteright}s seafloor observatory to investigate and monitor hydrographic, geophysical, geological, geochemical and biological processes of the hydrocarbon system, northern Gulf of Mexico. Innovative survey and monitoring systems, sensors, and tools have been developed to extract samples and data to unravel the history, character and composition of the site. Many hours of visual data have been collected to investigate benthic communities thriving at the cold seep site associated with the mound. These communities{\textquoteright} habitats are described here, for the first time, in terms of faunal assemblage, substrate nature, and presence/absence of chemosynthetic species. Based on these factors, we grouped them into four benthic meso-habitats. We speculate that the spatial distribution of these meso-habitats is large enough to make this characteristic comparable to the geophysical response of the seismo-acoustic systems. We have tested this hypothesis carefully analyzing the relationship between benthic habitats zonation and the geophysical response of Side Scan Sonar, Chirp Subbottom, Surface Source Deep Receiver (SSDR) vertical incidence profiler and 3-D oil industry multichannel data. We observe that the geophysical response is not unique, a single habitat may correlate with many geophysical attributes, or a single geophysical attribute may span many habitats. However, we find that geophysical data can predict seep locations. They can also convey some information concerning community composition and complexity that function as proxies for seep duration/age while specific community components are believed to reflect composition of seep fluids. Although preliminary, this approach represents a novel classification/characterization for seafloor hydrocarbon seeps, one that reflects a historical component.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.margeo.2016.03.016},
	author = {Macelloni, L and Lutken, C B and Ingrassia, M and D{\textquoteright} Emidio, M and Pizzi, M}
}
@article {ISI:000366671300021,
	title = {{Metagenomic resolution of microbial functions in deep-sea hydrothermal plumes across the Eastern Lau Spreading Center}},
	journal = {ISME JOURNAL},
	volume = {10},
	number = {1},
	year = {2016},
	month = {jan},
	pages = {225{\textendash}239},
	type = {Article},
	abstract = {Microbial processes within deep-sea hydrothermal plumes affect ocean biogeochemistry on global scales. In rising hydrothermal plumes, a combination of microbial metabolism and particle formation processes initiate the transformation of reduced chemicals like hydrogen sulfide, hydrogen, methane, iron, manganese and ammonia that are abundant in hydrothermal vent fluids. Despite the biogeochemical importance of this rising portion of plumes, it is understudied in comparison to neutrally buoyant plumes. Here we use metagenomics and bioenergetic modeling to describe the abundance and genetic potential of microorganisms in relation to available electron donors in five different hydrothermal plumes and three associated background deep-sea waters from the Eastern Lau Spreading Center located in the Western Pacific Ocean. Three hundred and thirty one distinct genomic {\textquoteleft}bins{\textquoteright} were identified, comprising an estimated 951 genomes of archaea, bacteria, eukarya and viruses. A significant proportion of these genomes is from novel microorganisms and thus reveals insights into the energy metabolism of heretofore unknown microbial groups. Community-wide analyses of genes encoding enzymes that oxidize inorganic energy sources showed that sulfur oxidation was the most abundant and diverse chemolithotrophic microbial metabolism in the community. Genes for sulfur oxidation were commonly present in genomic bins that also contained genes for oxidation of hydrogen and methane, suggesting metabolic versatility in these microbial groups. The relative diversity and abundance of genes encoding hydrogen oxidation was moderate, whereas that of genes for methane and ammonia oxidation was low in comparison to sulfur oxidation. Bioenergetic-thermodynamic modeling supports the metagenomic analyses, showing that oxidation of elemental sulfur with oxygen is the most dominant catabolic reaction in the hydrothermal plumes. We conclude that the energy metabolism of microbial communities inhabiting rising hydrothermal plumes is dictated by the underlying plume chemistry, with a dominant role for sulfur-based chemolithoautotrophy.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1751-7362},
	doi = {10.1038/ismej.2015.81},
	author = {Anantharaman, Karthik and Breier, John A and Dick, Gregory J}
}
@article {ISI:000372364000020,
	title = {{Metatranscriptomic insights into polyphosphate metabolism in marine sediments}},
	journal = {ISME JOURNAL},
	volume = {10},
	number = {4},
	year = {2016},
	month = {apr},
	pages = {1015{\textendash}1019},
	type = {Article},
	abstract = {Microorganisms can influence inorganic phosphate (P-i) in pore waters, and thus the saturation state of phosphatic minerals, by accumulating and hydrolyzing intracellular polyphosphate (poly-P). Here we used comparative metatranscriptomics to explore microbial poly-P utilization in marine sediments. Sulfidic marine sediments from methane seeps near Barbados and from the Santa Barbara Basin (SBB) oxygen minimum zone were incubated under oxic and anoxic sulfidic conditions. Pi was sequestered under oxic conditions and liberated under anoxic conditions. Transcripts homologous to poly-P kinase type 2 (ppk2) were 6-22 x more abundant in metatranscriptomes from the anoxic incubations, suggesting that reversible poly-P degradation by Ppk2 may be an important metabolic response to anoxia by marine microorganisms. Overall, diverse taxa differentially expressed homologues of genes for poly-P degradation (ppk2 and exopolyphosphatase) under different incubation conditions. Sulfur-oxidizing microorganisms appeared to preferentially express genes for poly-P degradation under anoxic conditions, which may impact phosphorus cycling in a wide range of oxygen-depleted marine settings.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1751-7362},
	doi = {10.1038/ismej.2015.169},
	author = {Jones, Daniel S and Flood, Beverly E and Bailey, Jake V}
}
@article {ISI:000373321200001,
	title = {{Nanocalorimetric Characterization of Microbial Activity in Deep Subsurface Oceanic Crustal Fluids}},
	journal = {FRONTIERS IN MICROBIOLOGY},
	volume = {7},
	year = {2016},
	month = {apr},
	type = {Article},
	abstract = {Although fluids within the upper oceanic basaltic crust harbor a substantial fraction of the total prokaryotic cells on Earth, the energy needs of this microbial population are unknown. In this study, a nanocalorimeter (sensitivity down to 1.2 nW ml(-1)) was used to measure the enthalpy of microbially catalyzed reactions as a function of temperature in samples from two distinct crustal fluid aquifers. Microorganisms in unamended, warm (63 degrees C) and geochemically altered anoxic fluids taken from 292 meters sub-basement (msb) near the Juan de Fuca Ridge produced 267.3 mJ of heat over the course of 97 h during a step-wise isothermal scan from 35.5 to 85.0 degrees C. Most of this heat signal likely stems from the germination of thermophilic endospores (6.66 x 10(4) cells ml(-1) FLUID) and their subsequent metabolic activity at temperatures greater than 50 degrees C. The average cellular energy consumption (5.68 pW cell(-1)) reveals the high metabolic potential of a dormant community transported by fluids circulating through the ocean crust. By contrast, samples taken from 293 msb from cooler (3.8 degrees C), relatively unaltered oxic fluids, produced 12.8 mJ of heat over the course of 14 h as temperature ramped from 34.8 to 43.0 degrees C. Corresponding cell-specific energy turnover rates (0.18 pW cell(-1)) were converted to oxygen uptake rates of 24.5 nmol O-2 ml(-1) FLUID d(-1), validating previous model predictions of microbial activity in this environment. Given that the investigated fluids are characteristic of expansive areas of the upper oceanic crust, the measured metabolic heat rates can be used to constrain boundaries of habitability and microbial activity in the oceanic crust.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1664-302X},
	doi = {10.3389/fmicb.2016.00454},
	author = {Robador, Alberto and LaRowe, Douglas E and Jungbluth, Sean P and Lin, Huei-Ting and Rappe, Michael S and Nealson, Kenneth H and Amend, Jan P}
}
@article {Hendrickx2016,
	title = {{New records of the deep-sea anemone Phelliactis callicyclus Riemann-Zurneck, 1973 (Cnidaria, Actiniaria, Hormathiidae) from the Gulf of California, Mexico}},
	journal = {Zootaxa},
	volume = {4178},
	year = {2016},
	pages = {145{\textendash}150},
	abstract = {Specimens of a deep-sea anemone were observed in photographs and video footage taken with the Remotely Operated Vehicle JASON (WHOI Deep Submergence Laboratory) in the Gulf of California, Mexico, in May 2008. Comparison of our material with photographs and description of this species available in literature indicate that the sea anemones filmed during the JASON survey are most likely to represent Phelliactis callicyclus Riemann-Zurneck, 1973. This species has previously been reported from a locality in the Gulf of California near the present record. During the JASON survey, 28 specimens of P. callicyclus were spotted in 27 locations during six dives. The specimens occurred on angular rock outcrops along the escarpments of the transform faults of the Gulf of California, between depths of 993-2543 m and at temperatures ranging from 2.3 to 4.5 degrees C. Based on these new records, Phelliactis callicyclus appears to be widely spread in the Gulf of California.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.11646/zootaxa.4178.1.8},
	author = {Hendrickx, M E and Hinojosa-Corona, A and Ayon-Parente, M}
}
@article {1555,
	title = {{Newly upgraded ROV Jason: Bigger and better}},
	journal = {Ocean News {\&} Technology},
	number = {May},
	year = {2016},
	pages = {42{\textendash}43},
	abstract = {A major, {\$}2.4 million upgrade funded by the National Science Foundation has made the ROV Jason more capable than ever. This 12-month-long project, conducted by engineers at WHOI, which designed and built the vehicle, has increased the vehicle payload and range of activities and streamlined the vehicle operation. This is Jason{\textquoteright}s first upgrade of this magnitude since its second-generation launch in 2002; the original Jason was launched in 1988. The ROV is operated by WHOI for the nation{\textquoteright}s ocean scientists as part of the National Deep Submergence Facility. The upgrade has other added benefits as well. The heavy lift capability will be used at seafloor sites where several-hundred-meters-long sensor strings are routinely deployed into and recovered from seafloor bore holes.},
	keywords = {ROV Jason (Remotely Operated Vehicle)}
}
@article {Jungbluth2016,
	title = {{Novel microbial assemblages inhabiting crustal fluids within mid-ocean ridge flank subsurface basalt}},
	journal = {ISME Journal},
	volume = {10},
	year = {2016},
	pages = {2033{\textendash}2047},
	abstract = {Although little is known regarding microbial life within our planet{\textquoteright}s rock-hosted deep subseafloor biosphere, boreholes drilled through deep ocean sediment and into the underlying basaltic crust provide invaluable windows of access that have been used previously to document the presence of microorganisms within fluids percolating through the deep ocean crust. In this study, the analysis of 1.7 million small subunit ribosomal RNA genes amplified and sequenced from marine sediment, bottom seawater and basalt-hosted deep subseafloor fluids that span multiple years and locations on the Juan de Fuca Ridge flank was used to quantitatively delineate a subseafloor microbiome comprised of distinct bacteria and archaea. Hot, anoxic crustal fluids tapped by newly installed seafloor sampling observatories at boreholes U1362A and U1362B contained abundant bacterial lineages of phylogenetically unique Nitrospirae, Aminicenantes, Calescamantes and Chloroflexi. Although less abundant, the domain Archaea was dominated by unique, uncultivated lineages of marine benthic group E, the Terrestrial Hot Spring Crenarchaeotic Group, the Bathyarchaeota and relatives of cultivated, sulfate-reducing Archaeoglobi. Consistent with recent geochemical measurements and bioenergetic predictions, the potential importance of methane cycling and sulfate reduction were imprinted within the basalt-hosted deep subseafloor crustal fluid microbial community. This unique window of access to the deep ocean subsurface basement reveals a microbial landscape that exhibits previously undetected spatial heterogeneity.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1038/ismej.2015.248},
	author = {Jungbluth, S P and Bowers, R M and Lin, H-T and Cowen, J P and Rappe, M S}
}
@article {ISI:000372166500012,
	title = {{Oceanographic patterns and carbonate chemistry in the vicinity of cold-water coral reefs in the Gulf of Mexico: Implications for resilience in a changing ocean}},
	journal = {LIMNOLOGY AND OCEANOGRAPHY},
	volume = {61},
	number = {2},
	year = {2016},
	month = {mar},
	pages = {648{\textendash}665},
	type = {Article},
	abstract = {To accurately assess the threat that global climate change poses to marine systems, a detailed baseline of the current carbonate chemistry and other oceanographic conditions is required. Despite the heightened vulnerability of deep-sea communities to ocean acidification, there have been relatively few studies investigating the carbonate chemistry immediately above cold-water coral reefs. Here, we present data collected during five cruises from 2010 to 2014 in the northern Gulf of Mexico and quantify the carbonate system and other oceanographic parameters in offshore surface-waters, the water column, and at deep benthic sites. Benthic sites containing the scleractinian cold-water coral L. pertusa occurred in waters with a relatively wide temperature range (6.8-13.6 degrees C), low potential density (sigma(theta)=26.9 +/- 0.3 kg m(-3)), low dissolved oxygen concentration (111.3 +/- 2.0 mu mol kg(-1)), low pH(T) (7.87 +/- 0.04), low Omega(ARAG) (1.31 +/- 0.14), and a low availability of carbonate ions (94.4 +/- 9.2 mu mol kg(-1)) compared with L. pertusa habitats in other regions. Based on previous modelling and experimental results, these values place L. pertusa at the edge of its viable niche in the deep Gulf of Mexico. However, significantly elevated total alkalinity (+139-44 mu mol kg(-1)) was detected above large L. pertusa mounds, suggesting that carbonate dissolution within the mounds may be partially ameliorating the direct effects of ocean acidification. Together, these results provide an important baseline for assessing future oceanographic changes in the Gulf of Mexico and for predicting the resilience of cold-water coral reefs to global climate and ocean change.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0024-3590},
	doi = {10.1002/lno.10242},
	author = {Georgian, Samuel E and DeLeo, Danielle and Durkin, Alanna and Gomez, Carlos E and Kurman, Melissa and Lunden, Jay J and Cordes, Erik E}
}
@article {ISI:000378670800005,
	title = {{Patterns and variability in geochemical signatures and microbial activity within and between diverse cold seep habitats along the lower continental slope, Northern Gulf of Mexico}},
	journal = {DEEP-SEA RESEARCH PART II-TOPICAL STUDIES IN OCEANOGRAPHY},
	volume = {129},
	year = {2016},
	month = {jul},
	pages = {31{\textendash}40},
	type = {Article},
	abstract = {We collected 69 sediment cores from distinct ecological and geological settings along the deep slope in the Northern Gulf of Mexico to evaluate whether specific geochemical- or habitat-related factors correlated with rates of microbial processes and geochemical signatures. By collecting replicate cores from distinct habitats across multiple sites, we illustrate and quantify the heterogeneity of cold seep geochemistry and microbial activity. These data also document the factors driving unique aspects of the geochemistry of deep slope gas, oil and brine seeps. Surprisingly little variation was observed between replicate (n=2-5) cores within sites for most analytes (except methane), implying that the common practice of collecting one core for geochemical analysis can capture the signature of a habitat in most cases. Depth-integrated concentrations of methane, dissolved inorganic carbon (DIC), and calcium were the predominant geochemical factors that correlated with a site{\textquoteright}s ecological or geological settings. Pore fluid methane concentration was related to the phosphate and DIC concentration, as well as to rates of sulfate reduction. While distinctions between seep habitats were identified from geochemical signatures, habitat specific geochemistry varied little across sites. The relative concentration of dissolved inorganic nitrogen versus phosphorus suggests that phosphorus availability limits biomass production at cold seeps. Correlations between calcium, chloride, and phosphate concentrations were indicative of brine-associated phosphate transport, suggesting that in addition to the co-migration of methane, dissolved organic carbon, and ammonium with brine, phosphate delivery is also associated with brine advection. (C) 2016 Published by Elsevier Ltd.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0645},
	doi = {10.1016/j.dsr2.2016.02.011},
	author = {Bowles, Marshall and Hunter, Kimberley S and Samarkin, Vladimir and Joye, Samantha}
}
@article {Garcia2016,
	title = {{Petrology and geochronology of lavas from Ka{\textquoteleft}ula Volcano: Implications for rejuvenated volcanism of the Hawaiian mantle plume}},
	journal = {Geochimica et Cosmochimica Acta},
	volume = {185},
	year = {2016},
	pages = {278{\textendash}301},
	abstract = {Marine surveying and submersible sampling of Ka{\textquoteleft}ula Volcano, located 100 km off the axis of the Hawaiian chain, revealed widespread areas of young volcanism. New 40Ar/39Ar and geochemical analyses of the olivine-phyric submarine and subaerial volcanic rocks show that Ka{\textquoteleft}ula is shrouded with 1.9{\textendash}0.5 Ma alkalic basalts. The ages and chemistry of these rocks overlap with rejuvenated lavas on nearby, northern Hawaiian Island shields (Ni{\textquoteleft}ihau, Kaua{\textquoteleft}i and South Kaua{\textquoteleft}i Swell). Collectively, these rejuvenated lavas cover a vast area (\~{}7000 km2), much more extensive than any other area of rejuvenated volcanism worldwide. Ka{\textquoteleft}ula rejuvenated lavas range widely in alkalinity and incompatible element abundances (e.g., up to 10{\texttimes} P2O5 at a given MgO value) and ratios indicating variable degrees of melting of a heterogeneous source. Heavy REE elements in Ka{\textquoteleft}ula lavas are pinned at a mantle normalized Yb value of 10 {\textpm} 1, reflecting the presence of garnet in the source. Trace element ratios indicate the source also contained phlogopite and an Fe{\textendash}Ti oxide. The new Ka{\textquoteleft}ula ages show that rejuvenated volcanism was nearly coeval from \~{}0.3 to 0.6 Ma along a 450 km segment of the Hawaiian Islands (from West Maui to north of Ka{\textquoteleft}ula). The ages and volumes for rejuvenated volcanism are inconsistent with all but one geodynamic melting model proposed to date. This model advocates a significant contribution of pyroxenite to rejuvenated magmas. Analyses of olivine phenocryst compositions suggest a major (33{\textendash}69{\%}) pyroxenite component in Ka{\textquoteleft}ula rejuvenated lavas, which correlates positively with radiogenic Pb isotope ratios for Ka{\textquoteleft}ula. This correlation is also observed in lavas from nearby South Kaua{\textquoteleft}i lavas, as was reported for Atlantic oceanic islands. The presence of pyroxenite in the source may have extended the duration and volume of Hawaiian rejuvenated volcanism.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.gca.2016.03.025},
	author = {Garcia, M O and Weis, D and Jicha, B R and Ito, G and Hanano, D}
}
@mastersthesis {McNichol2016,
	title = {{Productivity, metabolism and physiology of free-living chemoautotrophic Epsilonproteobacteria}},
	year = {2016},
	type = {phd},
	abstract = {Chemoautotrophic ecosystems at deep-sea hydrothermal vents were discovered in 1977, but not until 1995 were free-living autotrophic Epsilonproteobacteria identified as important microbial community members. Because the deep-sea is food-starved, the autotrophic metabolism of hydrothermal vent Epsilonproteobacteria may be very important for deep-sea consumers. However, quantifying their metabolic activities in situ has remained difficult, and biochemical mechanisms underlying their autotrophic physiology are poorly described. To gain insight into environmental processes, an approach was developed for incubations of microbes at in situ pressure and temperature (25 MPa, 24{\textdegree}C) with various combinations of electron donors/acceptors (H2, O2 and NO3 and 13HCO3) as a tracer to track carbon fixation. During short (18-24 h) incubations of low-temperature vent fluids from Crab Spa (9{\textdegree}N East Pacific Rise), the concentration of electron donors/acceptors and cell numbers were monitored to quantify microbial processes. Measured rates were generally higher than previous studies, and the stoichiometry of microbially-catalyzed redox reactions revealed new insights into sulfur and nitrogen cycling. Single-cell, taxonomically-resolved tracer incorporation showed Epsilonproteobacteria dominated carbon fixation, and their growth efficiency was calculated based on electron acceptor consumption. Using these data, in situ primary productivity, microbial standing stock, and average biomass residence time of the deep-sea vent subseafloor biosphere were estimated. Finally, the population structures of the most abundant genera Sulfurimonas and Thioreductor were shown to be strongly influenced by pO2 and temperature respectively, providing a mechanism for niche differentiation in situ. To gain insights into the core biochemical reactions underlying autotrophy in Epsilonprotebacteria, a theoretical metabolic model of Sulfurimonas denitrificans was developed. Validated iteratively by comparing in silico yields with data from chemostat experiments, the model generated hypotheses explaining critical, yet so far unresolved reactions supporting chemoautotrophy in Epsilonproteobacteria. For example, it provides insight into how energy is conserved during sulfur oxidation coupled to denitrification, how reverse electron transport produces ferredoxin for carbon fixation, and why aerobic growth yields are only slightly higher compared to denitrification. As a whole, this thesis provides important contributions towards understanding core mechanisms of chemoautrophy, as well as the in situ productivity, physiology and ecology of autotrophic Epsilonproteobacteria.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1575/1912/8450},
	author = {McNichol, J}
}
@article {Raven2016,
	title = {{Sedimentary pyrite $δ$34S differs from porewater sulfide in Santa Barbara Basin: Proposed role of organic sulfur}},
	journal = {Geochimica et Cosmochimica Acta},
	volume = {186},
	year = {2016},
	pages = {120{\textendash}134},
	abstract = {Santa Barbara Basin sediments host a complex network of abiotic and metabolic chemical reactions that knit together the carbon, sulfur, and iron cycles. From a 2.1-m sediment core collected in the center of the basin, we present high-resolution profiles of the concentrations and isotopic compositions of all the major species in this system: sulfate, sulfide (∑H2S), elemental sulfur (S0), pyrite, extractable organic sulfur (OS), proto-kerogen S, total organic and dissolved inorganic carbon, and total and reducible iron. Below 10 cm depth, the core is characterized by low apparent sulfate reduction rates ({\textless}0.01 mM/yr) except near the sulfate-methane transition zone. Surprisingly, pyrite forming in shallow sediments is \~{}30{\textperthousand} more 34S-depleted than coexisting ∑H2S in porewater. S0 has the same strongly 34S-depleted composition as pyrite where it forms near the sediment{\textendash}water interface, though not at depth. This pattern is not easily explained by conventional hypotheses in which sedimentary pyrite derives from abiotic reactions with porewater ∑H2S or from the products of S0 disproportionation. Instead, we propose that pyrite formation in this environment occurs within sulfate reducing microbial aggregates or biofilms, where it reflects the isotopic composition of the immediate products of bacterial sulfate reduction. Porewater ∑H2S in Santa Barbara Basin may be more 34S-enriched than pyrite due to equilibration with relatively 34S-enriched OS. The difference between OS and pyrite $δ$34S values would then reflect the balance between microbial sulfide formation and the abundance of exchangeable OS. Both OS and pyrite $δ$34S records thus have the potential to provide valuable information about biogeochemical cycles and redox structure in sedimentary paleoenvironments.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.gca.2016.04.037},
	author = {Raven, M R and Sessions, A L and Fischer, W W and Adkins, J F}
}
@article {Thal2016,
	title = {{Subaqueous cryptodome eruption, hydrothermal activity and related seafloor morphologies on the andesitic North Su volcano}},
	journal = {Journal of Volcanology and Geothermal Research},
	volume = {323},
	year = {2016},
	pages = {80{\textendash}96},
	abstract = {North Su is a double-peaked active andesite submarine volcano located in the eastern Manus Basin of the Bismarck Sea that reaches a depth of 1154 m. It hosts a vigorous and varied hydrothermal system with black and white smoker vents along with several areas of diffuse venting and deposits of native sulfur. Geologic mapping based on ROV observations from 2006 and 2011 combined with morphologic features identified from repeated bathymetric surveys in 2002 and 2011 documents the emplacement of a volcanic cryptodome between 2006 and 2011. We use our observations and rock analyses to interpret an eruption scenario where highly viscous, crystal-rich andesitic magma erupted slowly into the water-saturated, gravel-dominated slope of North Su. An intense fragmentation process produced abundant blocky clasts of a heterogeneous magma (olivine crystals within a rhyolitic groundmass) that only rarely breached through the clastic cover onto the seafloor. Phreatic and phreatomagmatic explosions beneath the seafloor cause mixing of juvenile and pre-existing lithic clasts and produce a volcaniclastic deposit. This volcaniclastic deposit consists of blocky, non-altered clasts next, variably (1{\textendash}100{\%}) altered clasts, hydrothermal precipitates and crystal fragments. The usually applied parameters to identify juvenile subaqueous lava fragments, i.e. fluidal shape or chilled margin, were not applicable to distinguish between pre-existing non-altered clasts and juvenile clasts. This deposit is updomed during further injection of magma and mechanical disruption. Gas-propelled turbulent clast-recycling causes clasts to develop variably rounded shapes. An abundance of blocky clasts and the lack of clasts typical for the contact of liquid lava with water is interpreted to be the result of a cooled, high-viscosity, crystal-rich magma that failed as a brittle solid upon stress. The high viscosity allows the lava to form blocky and short lobes. The pervasive volcaniclastic cover on North Su is partly cemented by hydrothermal precipitates. These hydrothermally-cemented breccias, crusts and single pillars show that hydrothermal circulation through a thick layer of volcaniclastic deposits can temporarily increase slope stability through precipitation and cementation.},
	keywords = {AUV ABE (Autonomous Benthic Explorer), ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.jvolgeores.2016.04.041},
	author = {Thal, J and Tivey, M A and Yoerger, D R and Bach, W}
}
@article {ISI:000380376700024,
	title = {{Subseafloor microbial communities in hydrogen-rich vent fluids from hydrothermal systems along the Mid-Cayman Rise}},
	journal = {ENVIRONMENTAL MICROBIOLOGY},
	volume = {18},
	number = {6, SI},
	year = {2016},
	month = {jun},
	pages = {1970{\textendash}1987},
	type = {Article},
	abstract = {Warm fluids emanating from hydrothermal vents can be used as windows into the rocky subseafloor habitat and its resident microbial community. Two new vent systems on the Mid-Cayman Rise each exhibits novel geologic settings and distinctively hydrogen-rich vent fluid compositions. We have determined and compared the chemistry, potential energy yielding reactions, abundance, community composition, diversity, and function of microbes in venting fluids from both sites: Piccard, the world{\textquoteright}s deepest vent site, hosted in mafic rocks; and Von Damm, an adjacent, ultramafic-influenced system. Von Damm hosted a wider diversity of lineages and metabolisms in comparison to Piccard, consistent with thermodynamic models that predict more numerous energy sources at ultramafic systems. There was little overlap in the phylotypes found at each site, although similar and dominant hydrogen-utilizing genera were present at both. Despite the differences in community structure, depth, geology, and fluid chemistry, energetic modelling and metagenomic analysis indicate near functional equivalence between Von Damm and Piccard, likely driven by the high hydrogen concentrations and elevated temperatures at both sites. Results are compared with hydrothermal sites worldwide to provide a global perspective on the distinctiveness of these newly discovered sites and the interplay among rocks, fluid composition and life in the subseafloor.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1462-2912},
	doi = {10.1111/1462-2920.13173},
	author = {Reveillaud, Julie and Reddington, Emily and McDermott, Jill and Algar, Christopher and Meyer, Julie L and Sylva, Sean and Seewald, Jeffrey and German, Christopher R and Huber, Julie A}
}
@article {ISI:000378670800006,
	title = {{Sustained in situ measurements of dissolved oxygen, methane and water transport processes in the benthic boundary layer at MC118, northern Gulf of Mexico}},
	journal = {DEEP-SEA RESEARCH PART II-TOPICAL STUDIES IN OCEANOGRAPHY},
	volume = {129},
	year = {2016},
	month = {jul},
	pages = {41{\textendash}52},
	type = {Article},
	abstract = {Within months of the BP Macondo Wellhead blowout, elevated methane concentrations within the water column revealed a significant retention of light hydrocarbons in deep waters plus corresponding dissolved oxygen (DO) deficits. However, chemical plume tracking efforts were hindered by a lack of in situ monitoring capabilities. Here, we describe results from in situ time-series, lander-based investigations of physical and biogeochemical processes controlling dissolved oxygen, and methane at Mississippi Canyon lease block 118 (similar to 18 km from the oil spill) conducted shortly after the blowout through April 2012. Multiple sensor arrays plus open-cylinder flux chambers ({\{}{\textquoteright}{\textquoteright}{\}}chimneys{\{}{\textquoteright}{\textquoteright}{\}}) deployed from a benthic lander collected oxygen, methane, pressure, and current speed and direction data within one meter of the seafloor. The ROVARD lander system was deployed for an initial 21-day test experiment (9/13/2010-10/04/2010) at 882 m depth before a longer 160-day deployment (10/24/2011-4/01/2012) at 884 m depth. Temporal variability in current directions and velocities and water temperatures revealed strong influences of bathymetrically steered currents and overlying along-shelf flows on local and regional water transport processes. DO concentrations and temperature were inversely correlated as a result of water mass mixing processes. Flux chamber measurements during the 160-day deployment revealed total oxygen utilization (TOU) averaging 11.6 mmol/m(2) day. Chimney DO concentrations measured during the 21-day deployment exhibited quasi-daily variations apparently resulting from an interaction between near inertial waves and the steep topography of an elevated scarp immediately adjacent to the 21-day deployment site that modulated currents at the top of the chimney. Variability in dissolved methane concentrations suggested significant temporal variability in gas release from nearby hydrocarbon seeps and/or delivery by local water transport processes. Free-vehicle (lander) monitoring over time scales of months to years utilizing in situ sensors can provide an understanding of processes controlling water transport, respiration and the fate and impacts of accidental and natural gas and oil releases. (C) 2015 Elsevier Ltd. All rights reserved.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0645},
	doi = {10.1016/j.dsr2.2015.11.012},
	author = {Martens, Christopher S and Mendlovitz, Howard P and Seim, Harvey and Lapham, Laura and D{\textquoteright}Emidio, Marco}
}
@article {ISI:000373276200001,
	title = {{Temperature and Redox Effect on Mineral Colonization in Juan de Fuca Ridge Flank Subsurface Crustal Fluids}},
	journal = {FRONTIERS IN MICROBIOLOGY},
	volume = {7},
	year = {2016},
	month = {mar},
	type = {Article},
	abstract = {To examine microbe-mineral interactions in subsurface oceanic crust, we evaluated microbial colonization on crustal minerals that were incubated in borehole fluids for 1 year at the seafloor wellhead of a crustal borehole observatory (IODP Hole U1301A, Juan de Fuca Ridge flank) as compared to an experiment that was not exposed to subsurface crustal fluids (at nearby IODP Hole U1301B). In comparison to previous studies at these same sites, this approach allowed assessment of the effects of temperature, fluid chemistry, and/or mineralogy on colonization patterns of different mineral substrates, and an opportunity to verify the approach of deploying colonization experiments at an observatory wellhead at the seafloor instead of within the borehole. The Hole U1301B deployment did not have biofilm growth, based on microscopy and DNA extraction, thereby confirming the integrity of the colonization design against bottom seawater intrusion. In contrast, the Hole U1301A deployment supported biofilms dominated by Epsilonproteobacteria (43.5{\%} of 370 16S rRNA gene clone sequences) and Gammaproteobacteria (29.3{\%}). Sequence analysis revealed overlap in microbial communities between different minerals incubated at the Hole U1301A wellhead, indicating that mineralogy did not separate biofilm structure within the 1-year colonization experiment. Differences in the Hole U1301A wellhead biofilm community composition relative to previous studies from within the borehole using similar mineral substrates suggest that temperature and the diffusion of dissolved oxygen through plastic components influenced the mineral colonization experiments positioned at the wellhead. This highlights the capacity of low abundance crustal fluid taxa to rapidly establish communities on diverse mineral substrates under changing environmental conditions such as from temperature and oxygen.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1664-302X},
	doi = {10.3339/fmicb.2016.00396},
	author = {Baquiran, Jean-Paul M and Ramirez, Gustavo A and Haddad, Amanda G and Toner, Brandy M and Hulme, Samuel and Wheat, Charles G and Edwards, Katrina J and Orcutt, Beth N}
}
@article {ISI:000367801400177,
	title = {{Trophosome of the Deep-Sea Tubeworm Riftia pachyptila Inhibits Bacterial Growth}},
	journal = {PLOS ONE},
	volume = {11},
	number = {1},
	year = {2016},
	month = {jan},
	type = {Article},
	abstract = {The giant tubeworm Riftia pachyptila lives in symbiosis with the chemoautotrophic gamma-proteobacterium Cand. Endoriftia persephone. Symbionts are released back into the environment upon host death in high-pressure experiments, while microbial fouling is not involved in trophosome degradation. Therefore, we examined the antimicrobial effect of the tubeworm{\textquoteright}s trophosome and skin. The growth of all four tested Gram-positive, but only of one of the tested Gram-negative bacterial strains was inhibited by freshly fixed and degrading trophosome (incubated up to ten days at either warm or cold temperature), while no effect on Saccharomyces cerevisiae was observed. The skin did not show antimicrobial effects. A liquid chromatography-mass spectrometric analysis of the ethanol supernatant of fixed trophosomes lead to the tentative identification of the phospholipids 1-palmitoleyl-2-lyso- phosphatidylethanolamine, 2-palmitoleyl-1-lyso-phosphatidylethanolamine and the free fatty acids palmitoleic, palmitic and oleic acid, which are known to have an antimicrobial effect. As a result of tissue autolysis, the abundance of the free fatty acids increased with longer incubation time of trophosome samples. This correlated with an increasing growth inhibition of Bacillus subtilis and Listeria welshimeri, but not of the other bacterial strains. Therefore, the free fatty acids produced upon host degradation could be the cause of inhibition of at least these two bacterial strains.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1932-6203},
	doi = {10.1371/journal.pone.0146446},
	author = {Klose, Julia and Aistleitner, Karin and Horn, Matthias and Krenn, Liselotte and Dirsch, Verena and Zehl, Martin and Bright, Monika}
}
@article {Radice2016,
	title = {{Vertical water mass structure in the North Atlantic influences the bathymetric distribution of species in the deep-sea coral genus Paramuricea}},
	journal = {Deep Sea Research Part I: Oceanographic Research Papers},
	volume = {116},
	year = {2016},
	pages = {253{\textendash}263},
	abstract = {Deep-sea corals are the structural foundation of their ecosystems along continental margins worldwide, yet the factors driving their broad distribution are poorly understood. Environmental factors, especially depth-related variables including water mass properties, are thought to considerably affect the realized distribution of deep-sea corals. These factors are governed by local and regional oceanographic conditions that directly influence the dispersal of larvae, and therefore affect the ultimate distribution of adult corals. We used molecular barcoding of mitochondrial and nuclear sequences to identify species of octocorals in the genus Paramuricea collected from the Labrador Sea to the Grand Banks of Newfoundland, Canada at depths of 150{\textendash}1500 m. The results of this study revealed overlapping bathymetric distributions of the Paramuricea species present off the eastern Canadian coast, including the presence of a few cryptic species previously designated as Paramuricea placomus. The distribution of Paramuricea species in the western North Atlantic differs from the Gulf of Mexico, where five Paramuricea species exhibit strong segregation by depth. The different patterns of Paramuricea species in these contrasting biogeographic regions provide insight into how water mass structure may shape species distribution. Investigating Paramuricea prevalence and distribution in conjunction with oceanographic conditions can help demonstrate the factors that generate and maintain deep-sea biodiversity.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.dsr.2016.08.014},
	author = {Radice, V Z and Quattrini, A M and Wareham, V E and Edinger, E N and Cordes, E E}
}
@article {Chadwick2016,
	title = {{Voluminous eruption from a zoned magma body after an increase in supply rate at Axial Seamount}},
	journal = {Geophysical Research Letters},
	volume = {43},
	year = {2016},
	pages = {12,063{\textendash}12,070},
	abstract = {Axial Seamount is the best monitored submarine volcano in the world, providing an exceptional window into the dynamic interactions between magma storage, transport, and eruption processes in a mid-ocean ridge setting. An eruption in April 2015 produced the largest volume of erupted lava since monitoring and mapping began in the mid-1980s after the shortest repose time, due to a recent increase in magma supply. The higher rate of magma replenishment since 2011 resulted in the eruption of the most mafic lava in the last 500{\textendash}600 years. Eruptive fissures at the volcano summit produced pyroclastic ash that was deposited over an area of at least 8 km2. A systematic spatial distribution of compositions is consistent with a single dike tapping different parts of a thermally and chemically zoned magma reservoir that can be directly related to previous multichannel seismic-imaging results.},
	keywords = {AUV Sentry (Autonomous Underwater Vehicle), ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1002/2016GL071327},
	author = {Chadwick, W W and Paduan, J B and Clague, D A and Dreyer, B M and Merle, S G and Bobbitt, A M and Caress, D W and Philip, B T and Kelley, D S and Nooner, S L}
}
@article {ISI:000348807500001,
	title = {{Activity and phylogenetic diversity of sulfate-reducing microorganisms in low-temperature subsurface fluids within the upper oceanic crust}},
	journal = {FRONTIERS IN MICROBIOLOGY},
	volume = {5},
	year = {2015},
	month = {jan},
	type = {Article},
	abstract = {The basaltic ocean crust is the largest aquifer system on Earth, yet the rates of biological activity in this environment are unknown. Low-temperature ({\textless}100 degrees C) fluid samples were investigated from two borehole observatories in the Juan de Fuca Ridge (JFR) flank, representing a range of upper oceanic basement thermal and geochemical properties. Microbial sulfate reduction rates (SRR) were measured in laboratory incubations with S-35-sulfate over a range of temperatures and the identity of the corresponding sulfate-reducing microorganisms (SRM) was studied by analyzing the sequence diversity of the functional marker dissimilatory (bi)sulfite reductase (dsrAB) gene. We found that microbial sulfate reduction was limited by the decreasing availability of organic electron donors in higher temperature, more altered fluids. Thermodynamic calculations indicate energetic constraints for metabolism, which together with relatively higher cell-specific SRR reveal increased maintenance requirements, consistent with novel species-level dsrAB phylotypes of thermophilic SRM. Our estimates suggest that microbially-mediated sulfate reduction may account for the removal of organic matter in fluids within the upper oceanic crust and underscore the potential quantitative impact of microbial processes in deep subsurface marine crustal fluids on marine and global biogeochemical carbon cycling.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1664-302X},
	doi = {10.3389/fmicb.2014.00748},
	author = {Robador, Alberto and Jungbluth, Sean P and LaRowe, Douglas E and Bowers, Robert M and Rappe, Michael S and Amend, Jan P and Cowen, James P}
}
@article {ISI:000360888000001,
	title = {{Carbon fixation by basalt-hosted microbial communities}},
	journal = {FRONTIERS IN MICROBIOLOGY},
	volume = {6},
	year = {2015},
	month = {sep},
	type = {Article},
	abstract = {Oceanic crust is a massive potential habitat for microbial life on Earth, yet our understanding of this ecosystem is limited due to difficulty in access. In particular, measurements of rates of microbial activity are sparse. We used stable carbon isotope incubations of crustal samples, coupled with functional gene analyses, to examine the potential for carbon fixation on oceanic crust. Both seafloor-exposed and subseafloor basalts were recovered from different mid-ocean ridge and hot spot environments (i.e., the Juan de Fuca Ridge, the Mid-Atlantic Ridge, and the Loihi Seamount) and incubated with C-13-labeled bicarbonate. Seafloor-exposed basalts revealed incorporation of C-13-label into organic matter over time, though the degree of incorporation was heterogeneous. The incorporation of C-13 into biomass was inconclusive in subseafloor basalts. Translating these measurements into potential rates of carbon fixation indicated that 0.1-10 nmol C g(-1) rock d(-1) could be fixed by seafloor-exposed rocks. When scaled to the global production of oceanic crust, this suggests carbon fixation rates of 10(9)-10(12) g C year(-1), which matches earlier predictions based on thermodynamic calculations. Functional gene analyses indicate that the Calvin cycle is likely the dominant biochemical mechanism for carbon fixation in basalt-hosted biofilms, although the reductive acetyl-CoA pathway and reverse TCA cycle likely play some role in net carbon fixation. These results provide empirical evidence for autotrophy in oceanic crust, suggesting that basalt-hosted autotrophy could be a significant contributor of organic matter in this remote and vast environment.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {1664-302X},
	doi = {10.3389/fmicb.2015.90400904},
	author = {Orcutt, Beth N and Sylvan, Jason B and Rogers, Daniel R and Delaney, Jennifer and Lee, Raymond W and Girguis, Peter R}
}
@article {ISI:000350088800011,
	title = {{Characterization of vent fauna at the Mid-Cayman Spreading Center}},
	journal = {DEEP-SEA RESEARCH PART I-OCEANOGRAPHIC RESEARCH PAPERS},
	volume = {97},
	year = {2015},
	month = {mar},
	pages = {124{\textendash}133},
	type = {Article},
	abstract = {Hydrothermal vents in the deep sea have a global distribution on mid-ocean ridges and comprise at least six biogeographic provinces. A geographically isolated vent system was recently discovered on the Mid-Cayman Spreading Center (MCSC). Here, we describe the faunal assemblages associated with this system and their relationship to known biogeographic provinces. Taxa from MCSC vents were sorted based on morphology and barcoded using the cytochrome oxidase I (COI) and 16S ribosomal RNA (16S) genes for identification. Distinct faunal assemblages were recognized around vent chimneys at two hydrothermal vent fields (Von Damm and Beebe) separated by a distance of similar to 13 km and {\textgreater} 2.5-km depth along the Mid-Cayman Spreading Center. These results suggest that depth and/or local conditions structure faunal assemblages in this region. COI and microsatellite markers were then used to explore the genetic structure of the shrimp Rimicaris hybisae, the only abundant species shared between the shallow Von Damm and the deep Beebe vent fields. R. hybisae was not genetically differentiated between the Von Damm Spire and Beebe chimneys, suggesting this species is better adapted for bathymetric dispersal and the differences in local conditions than other MCSC species. In addition, a third faunal assemblage dominated by two species of tubeworms was identified at Von Damm in association with weakly diffuse flow sites (including the site known as {\textquoteleft}{\textquoteleft}Marker X18{\{}{\textquoteright}{\textquoteright}{\}}). The Marker X18 assemblage shares species with seeps in the region. Fauna shared with both vents and seeps at the MCSC reinforces the need for a global biogeographic study of deep-sea chemosynthetic fauna that is not focused on specific habitats. (C) 2014 Elsevier Ltd. All rights reserved.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0637},
	doi = {10.1016/j.dsr.2014.11.011},
	author = {Plouviez, Sophie and Jacobson, Alixandra and Wu, Mengyou and Van Dover, Cindy L}
}
@article {Escoube2015,
	title = {{Coupled Ge/Si and Ge isotope ratios as geochemical tracers of seafloor hydrothermal systems: Case studies at Loihi Seamount and East Pacific Rise 9{\textdegree}50'N}},
	journal = {Geochimica et Cosmochimica Acta},
	volume = {167},
	year = {2015},
	pages = {93{\textendash}112},
	abstract = {Germanium (Ge) and Silicon (Si) exhibit similar geochemical behavior in marine environments but are variably enriched in seafloor hydrothermal fluids relative to seawater. In this study, Ge isotope and Ge/Si ratio systematics were investigated in low temperature hydrothermal vents from Loihi Seamount (Pacific Ocean, 18{\textdegree}54'N, 155{\textdegree}15'W) and results were compared to high-temperature vents from the East Pacific Rise (EPR) at 9{\textdegree}50'N. Loihi offers the opportunity to understand contrasting Ge and Si behavior in low temperature seafloor hydrothermal systems characterized by abundant Fe oxyhydroxide deposition at the seafloor. The results show that both Ge/Si and $δ$74/70Ge in hydrothermal fluids are fractionated relative to the basaltic host rocks. The enrichment in Ge vs. Si relative to fresh basalts, together with Ge isotope fractionation ($Δ$74/70Gefluid-basalt up to 1.15{\textperthousand} at EPR 9{\textdegree}50'N and 1.64{\textperthousand} at Loihi) are best explained by the precipitation of minerals (e.g. quartz and Fe-sulfides) during higher temperature seawater{\textendash}rock reactions in the subsurface. The study of Fe-rich hydrothermal deposits at Loihi, largely composed of Fe-oxyhydroxides, shows that Ge isotopes are also fractionated upon mineral precipitation at the seafloor. We obtained an average Ge isotope fractionation factor between Fe-oxyhydroxide (ferrihydrite) and dissolved Ge in the fluid of -2.0 {\textpm} 0.6{\textperthousand} (2sd), and a maximum value of -3.6 {\textpm} 0.6{\textperthousand} (2sd), which is consistent with recent theoretical and experimental studies. The study of a hydrothermal chimney at Bio 9 vent at EPR 9{\textdegree}50'N also demonstrates that Ge isotopes are fractionated by approximately -5.6 {\textpm} 0.6{\textperthousand} (2sd) during precipitation of metal sulfides under hydrothermal conditions. Using combined Ge/Si and estimated Ge isotope signatures of Ge sinks and sources in seawater, we propose a preliminary oceanic budget of Ge which reveals that an important sink, referred as the {\textquotedblleft}missing Ge sink{\textquotedblright}, may correspond to Ge sequestration into authigenic Fe-oxyhydroxides in marine sediments. This study shows that combining Ge/Si and $δ$74/70Ge systematics provides a useful tool to trace hydrothermal Ge and Si sources in marine environments and to understand formation processes of seafloor hydrothermal deposits.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.gca.2015.06.025},
	author = {Escoube, R and Rouxel, O J and Edwards, K J and Glazer, B and Donard, O F X}
}
@article {ISI:000349374800012,
	title = {{A deep-sea community, including Lophelia pertusa, at unusually shallow depths in the western North Atlantic Ocean off northeastern Florida}},
	journal = {MARINE BIOLOGY},
	volume = {162},
	number = {3},
	year = {2015},
	month = {mar},
	pages = {635{\textendash}648},
	type = {Article},
	abstract = {Living colonies of the cold-water scleractinian coral Lophelia pertusa and other typically deep-water organisms were discovered in unusually shallow depths (180-250 m) off northeastern Florida. Observations of L. pertusa on rocky substrata and coral-built mounds represent the shallowest records of large colonies of this coral in the western Atlantic Ocean. Bioherms up to 30 m tall, extensive areas of eroded L. pertusa rubble, and a well-developed cold-water community indicated that these sites are long-term features, rather than short-term opportunistic responses to temporary shifts in environmental conditions. Species that are commonly observed on deeper reefs off the southeastern USA were abundant at the shallow sites. The most abundant fishes on reef habitats were Helicolenus dactylopterus, Laemonema barbatulum, Dysommina rugosa, and Anthias spp. In addition to L. pertusa, the most common macroinvertebrates on hard substrata were Eumunida picta, Chaceon fenneri, octocorals, cup corals, and glass sponges. Bottom and near-bottom temperatures (7-10 A degrees C) and nutrient concentrations at the shallow sites were similar to those normally encountered at 500-600 m in this region. The shallow reef sites occur in an area known for frequent Gulf Stream-driven upwelling of deep, nutrient-rich water. However, the upwelling must be persistent or permanent in order to maintain deep-sea communities at such shallow depths. Based on these data, this area is under final review by the US Department of Commerce for inclusion in one of the regional Coral Habitat Areas of Particular Concern.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0025-3162},
	doi = {10.1007/s00227-015-2611-2},
	author = {Ross, Steve W and Brooke, Sandra and Quattrini, Andrea M and Rhode, Mike and Watterson, J Carter}
}
@article {Lin2015,
	title = {{Dissolved amino acids in oceanic basaltic basement fluids}},
	journal = {Geochimica et Cosmochimica Acta},
	volume = {164},
	year = {2015},
	pages = {175{\textendash}190},
	abstract = {The oceanic basaltic basement contains the largest aquifer on Earth and potentially plays an important role in the global carbon cycle as a net sink for dissolved organic carbon (DOC). However, few details of the organic matter cycling in the subsurface are known because great water depths and thick sediments typically hinder direct access to this environment. In an effort to examine the role of water{\textendash}rock{\textendash}microorganism interaction on organic matter cycling in the oceanic basaltic crust, basement fluid samples collected from three borehole observatories installed on the eastern flank of the Juan de Fuca Ridge were analyzed for dissolved amino acids. Our data show that dissolved free amino acids (1{\textendash}13 nM) and dissolved hydrolyzable amino acids (43{\textendash}89 nM) are present in the basement. The amino acid concentrations in the ridge-flank basement fluids are at the low end of all submarine hydrothermal fluids reported in the literature and are similar to those in deep seawater. Amino acids in recharging deep seawater, in situ amino acid production, and diffusional input from overlying sediments are potential sources of amino acids in the basement fluids. Thermodynamic modeling shows that amino acid synthesis in the basement can be sustained by energy supplied from inorganic substrates via chemolithotrophic metabolisms. Furthermore, an analysis of amino acid concentrations and compositions in basement fluids support the notion that heterotrophic activity is ongoing. Similarly, the enrichment of acidic amino acids and depletion of hydrophobic ones relative to sedimentary particulate organic matter suggests that surface sorption and desorption also alters amino acids in the basaltic basement. In summary, although the oceanic basement aquifer is a net sink for deep seawater DOC, similar amino acid concentrations in basement aquifer and deep seawater suggest that DOC is preferentially removed in the basement over dissolved amino acids. Our data also suggest that organic carbon cycling occurs in the oceanic basaltic basement, where an active subsurface biosphere is likely responsible for amino acid synthesis and degradation.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.gca.2015.04.044},
	author = {Lin, H-T and Amend, J P and LaRowe, D E and Bingham, J-P and Cowen, J P}
}
@article {ISI:000361755600008,
	title = {{Diversity and composition of the copepod communities associated with megafauna around a cold seep in the Gulf of Mexico with remarks on species biogeography}},
	journal = {MARINE BIODIVERSITY},
	volume = {45},
	number = {3},
	year = {2015},
	month = {sep},
	pages = {419{\textendash}432},
	type = {Article; Proceedings Paper},
	abstract = {In order to characterize the copepod communities associated with tubeworm and mussel aggregations around a hydrocarbon seep in the Green Canyon of the Gulf of Mexico, diversity, abundance, and community composition were analyzed. Also analyzed were species biogeography and the potential connectivity to other chemosynthesis-based habitats. Copepod abundance and biomass were very low among tubeworms and mussels, with 0.22 to 6.08 individuals per 10 cm(2) sampled area and 9.02 to 42.43 mu g wet weight 10 cm(2) sampled area, respectively; but, abundance was significantly higher among the mussels. Fifty-five copepod species were identified, of which most were newly discovered and primarily belonging to the Harpacticoida order. Four copepod species were previously recorded from other food-rich and hard-substrata environments, such as hydrothermal vents or wood falls. Another four species showed close morphological proximity to species described from cold seeps, hydrothermal vents, and wood falls. Copepod diversity and community composition showed no significant differences between the foundation species. However, differences in the relative abundance and dominance of single species indicate a rather homogeneous community in mussel beds and a more heterogeneous community among tubeworms, indicating that foundation species may shape the abundance and community composition of associated copepods at cold seeps.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {1867-1616},
	doi = {10.1007/s12526-014-0310-8},
	author = {Plum, Christoph and Gollner, Sabine and Martinez-Arbizu, Pedro and Bright, Monika}
}
@article {ISI:000348129100027,
	title = {{A Dwarf Male Reversal in Bone-Eating Worms}},
	journal = {CURRENT BIOLOGY},
	volume = {25},
	number = {2},
	year = {2015},
	month = {jan},
	pages = {236{\textendash}241},
	type = {Article},
	abstract = {Darwin {\{}[{\}}1] hypothesized that sexes in a species should be similar unless sexual selection, fecundity selection, or resource partitioning has driven them apart. Male dwarfism has evolved multiple times in a range of animals, raising questions about factors that drive such extreme size dimorphism {\{}[{\}}2-4]. Ghiselin {\{}[{\}}5] noted that dwarf males are more common among smaller marine animals, and especially among sedentary and sessile species living at low densities, where mates are difficult to find, or in deep-sea environments with limited energy sources. These benefits of male dwarfism apply well to Osedax (Annelida: Siboglinidae), bone-eating marine worms {\{}[{\}}6]. Osedax males, notable for extreme sexual size dimorphism (SSD), are developmentally arrested larvae that produce sperm from yolk reserves. Harems of dwarf males reside in the lumen of the tube surrounding a female. Herein, we describe Osedax priapus n. sp., a species that deviates remarkably by producing males that anchor into, and feed on, bone via symbiont-containing {\textquoteleft}{\textquoteleft}roots,{\{}{\textquoteright}{\textquoteright}{\}} just like female Osedax. Phylogenetic analyses revealed O. priapus n. sp. as a derived species, and the absence of dwarf males represents a character reversal for this genus. Some dwarf male features are retained due to functional and morphological constraints. Since O. priapus n. sp. males are anchored in bone, they possess an extensible trunk that allows them to roam across the bone to contact and inseminate females. Evolutionary and ecological implications of a loss of male dwarfism are discussed.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0960-9822},
	doi = {10.1016/j.cub.2014.11.032},
	author = {Rouse, Greg W and Wilson, Nerida G and Worsaae, Katrine and Vrijenhoek, Robert C}
}
@article {Bennett2015,
	title = {{Effect of depth and vent fluid composition on the carbon sources at two neighboring deep-sea hydrothermal vent fields (Mid-Cayman Rise)}},
	journal = {Deep-Sea Research Part I: Oceanographic Research Papers},
	volume = {104},
	year = {2015},
	pages = {122{\textendash}133},
	abstract = {In this study, we have used stable isotopes of megafauna, microbial mats and particulate organic matter to examine the effect of depth and vent fluid composition on the carbon sources at two proximal, chemically distinct hydrothermal vent fields along the Mid-Cayman Rise. The basalt hosted Piccard vent field (4980 m) is twice as deep as the ultramafic hosted Von Damm vent field (2300 m) and has very different faunal assemblages. Of particular note is the presence of seep-associated fauna, Escarpia and Lamellibrachia tubeworms, at the Von Damm vent field. We identify a greater range of carbon sources and a suggestion of increased photosynthetic inputs to the Von Damm vent field compared to Piccard vent field. Rimicaris hybisae shrimp are the only abundant species shared between the two vent fields with $δ$13C values ranging between -22.7 and -10.1{\textperthousand}. Higher concentrations of hydrogen sulfide in the vent fluids at Piccard is proposed to be responsible for varying the relative contributions of the carbon fixation cycles used by their epibionts. Seep-associated fauna at Von Damm rely on elevated, thermogenic hydrocarbon content of the vent fluids for their carbon source ($δ$13C values ranging from -21.3 to 11.6{\textperthousand}). They also derive energy from hydrogen sulfide formed by the microbial reduction of sulfide ($δ$34S values ranging from -10.2 to -6.9{\textperthousand}). The tubeworms have very short roots (buried at most a centimeter into rubble), suggesting that microbial sulfate reduction must be occurring either in the shallow subsurface and/or in the anterior part of the tube. Overall, megafauna at Von Damm vent field appear to have a smaller food chain length (smaller $δ$15N range) but a greater breadth of trophic resources compared to the megafauna at the Piccard vent field.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.dsr.2015.06.005},
	author = {Bennett, S A and Van Dover, C L and Breier, J A and Coleman, M L}
}
@article {ISI:000365379500011,
	title = {{Estimations of heat transfer from Grotto{\textquoteright}s North Tower: A NEPTUNE Observatory case study}},
	journal = {DEEP-SEA RESEARCH PART II-TOPICAL STUDIES IN OCEANOGRAPHY},
	volume = {121},
	number = {SI},
	year = {2015},
	month = {nov},
	pages = {95{\textendash}111},
	type = {Article},
	abstract = {The overall heat transfer through an active hydrothermal sulfide edifice, in particular the North Tower of Grotto, has been estimated at 80 MW or greater based on the following in situ measurements and assumptions: (1) The heat transfer by diffuse flow is estimated at 33-380 MW based on extrapolating the acoustically mapped area to all sides of the North Tower ({\{}{\textquoteright}{\textquoteright}{\}}visible{\{}{\textquoteright}{\textquoteright}{\}} area=30 m(2); extrapolated area= 100 m(2)) and using the range of available spot measurements of temperature (6-23 degrees C) and vertical velocity (0.07-0.28 m/s). The lower number (33 MW) is more likely, but there is insufficient knowledge of the temporal and spatial variability of diffuse flow to be certain. (2) The heat transfer by focused flow is estimated at 30-70 MW based on summing the estimated individual rates of heat transfer for 4 out of 7 documented black smokers and flanges. (3) Conductive heat transfer out of the mound is unknown, but is likely to be much less than the advective heat transfer. Additionally, the plume transport of heat is estimated at 20-40 MW based on the direct measurement of temperature within the plume (at 5-25 m above the top of the edifice). Despite uncertainties, the lower estimate of plume versus smoker heat transfer suggests that heat transfer is dominantly by diffuse flow. Furthermore, not all plumes from individual smokers may merge even for so small an area as the North Tower of Grotto. (C) 2015 Elsevier Ltd. All rights reserved.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0645},
	doi = {10.1016/j.dsr2.2015.05.010},
	author = {Rona, Peter A and Bemis, Karen G and Xu, Guangyu and Mitsuzawa, Kyohiko}
}
@article {ISI:000366805800006,
	title = {{{\textquoteleft}{\textquoteleft}Fingerprinting{\{}{\textquoteright}{\textquoteright}{\}} tectono-magmatic provenance using trace elements in igneous zircon}},
	journal = {CONTRIBUTIONS TO MINERALOGY AND PETROLOGY},
	volume = {170},
	number = {5-6},
	year = {2015},
	month = {dec},
	type = {Article},
	abstract = {Over 5300 recent SHRIMP-RG analyses of trace elements (TE) in igneous zircon have been compiled and classified based on their original tectono-magmatic setting to empirically evaluate {\textquoteleft}{\textquoteleft}geochemical fingerprints{\{}{\textquoteright}{\textquoteright}{\}} unique to those settings. Immobile element geochemical fingerprints used for lavas are applied with the same rational to zircon, including consideration of mineral competition on zircon TE ratios, and new criteria for distinguishing mid-ocean ridge (MOR), magmatic arc, and ocean island (and other plume-influenced) settings are proposed. The elemental ratios in zircon effective for fingerprinting tectono-magmatic provenance are systematically related to lava composition from equivalent settings. Existing discrimination diagrams using zircon U/Yb versus Hf or Y do not distinguish TE-enriched ocean island settings (i.e., Iceland, Hawaii) from magmatic arc settings. However, bivariate diagrams with combined cation ratios involving U-Nb-Sc-Yb-Gd-Ce provide a more complete distinction of zircon from these settings. On diagrams of U/Yb versus Nb/Yb, most MOR, ocean island, and kimberlite zircon define a broad {\textquoteleft}{\textquoteleft}mantle-zircon array{\{}{\textquoteright}{\textquoteright}{\}}; arc zircon defines a parallel array offset to higher U/Yb. Distinctly low U/Yb ratios of MOR zircon (typically {\textless}0.1) mirror their parental magmas and long-term incompatible element depletion of the MORB mantle. Plume-influenced sources are distinguished from MOR by higher U/Yb, U/Nb, Nb/Yb, and Nb/Sc. For zircon with U/Yb {\textgreater}0.1, high Sc/Yb separates arc settings from low-Sc/Yb plume-influenced sources. The slope of scandium enrichment trends in zircon differ between MOR and continental arc settings, likely reflecting the involvement of amphibole during melt differentiation. Scandium is thus also critical for discriminating provenance, but its behavior in zircon probably reflects contrasting melt fractionation trends between tholeiitic and calc-alkaline systems more than compositional differences in primitive magmas sourced at each tectono-magmatic source.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0010-7999},
	doi = {10.1007/s00410-015-1199-3},
	author = {Grimes, C B and Wooden, J L and Cheadle, M J and John, B E}
}
@article {Li2015,
	title = {{Genomic and transcriptomic evidence for scavenging of diverse organic compounds by widespread deep-sea archaea}},
	journal = {Nature Communications},
	volume = {6},
	year = {2015},
	pages = {8933},
	abstract = {Microbial activity is one of the most important processes to mediate the flux of organic carbon from the ocean surface to the seafloor. However, little is known about the microorganisms that underpin this key step of the global carbon cycle in the deep oceans. Here we present genomic and transcriptomic evidence that five ubiquitous archaeal groups actively use proteins, carbohydrates, fatty acids and lipids as sources of carbon and energy at depths ranging from 800 to 4,950 m in hydrothermal vent plumes and pelagic background seawater across three different ocean basins. Genome-enabled metabolic reconstructions and gene expression patterns show that these marine archaea are motile heterotrophs with extensive mechanisms for scavenging organic matter. Our results shed light on the ecological and physiological properties of ubiquitous marine archaea and highlight their versatile metabolic strategies in deep oceans that might play a critical role in global carbon cycling.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1038/ncomms9933},
	author = {Li, M and Baker, B J and Anantharaman, K and Jain, S and Breier, J A and Dick, G J}
}
@article {ISI:000351204900007,
	title = {{Genomic insights into the uncultivated marine Zetaproteobacteria at Loihi Seamount}},
	journal = {ISME JOURNAL},
	volume = {9},
	number = {4},
	year = {2015},
	month = {apr},
	pages = {857{\textendash}870},
	type = {Article},
	abstract = {The Zetaproteobacteria are a candidate class of marine iron-oxidizing bacteria that are typically found in high iron environments such as hydrothermal vent sites. As much remains unknown about these organisms due to difficulties in cultivation, single-cell genomics was used to learn more about this elusive group at Loihi Seamount. Comparative genomics of 23 phylogenetically diverse single amplified genomes (SAGs) and two isolates indicate niche specialization among the Zetaproteobacteria may be largely due to oxygen tolerance and nitrogen transformation capabilities. Only Form II ribulose 1,5-bisphosphate carboxylase (RubisCO) genes were found in the SAGs, suggesting that some of the uncultivated Zetaproteobacteria may be adapted to low oxygen and/or high carbon dioxide concentrations. There is also genomic evidence of oxygen-tolerant cytochrome c oxidases and oxidative stress-related genes, indicating that others may be exposed to higher oxygen conditions. The Zetaproteobacteria also have the genomic potential for acquiring nitrogen from numerous sources including ammonium, nitrate, organic compounds, and nitrogen gas. Two types of molybdopterin oxidoreductase genes were found in the SAGs, indicating that those found in the isolates, thought to be involved in iron oxidation, are not consistent among all the Zetaproteobacteria. However, a novel cluster of redox-related genes was found to be conserved in 10 SAGs as well as in the isolates warranting further investigation. These results were used to isolate a novel iron-oxidizing Zetaproteobacteria. Physiological studies and genomic analysis of this isolate were able to support many of the findings from SAG analyses demonstrating the value of these data for designing future enrichment strategies.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1751-7362},
	doi = {10.1038/ismej.2014.183},
	author = {Field, Erin K and Sczyrba, Alexander and Lyman, Audrey E and Harris, Christopher C and Woyke, Tanja and Stepanauskas, Ramunas and Emerson, David}
}
@article {McDermott2015,
	title = {{Identification of sulfur sources and isotopic equilibria in submarine hot-springs using multiple sulfur isotopes}},
	journal = {Geochimica et Cosmochimica Acta},
	volume = {160},
	year = {2015},
	pages = {169{\textendash}187},
	abstract = {Multiple sulfur isotopes were measured in metal sulfide deposits, elemental sulfur, and aqueous hydrogen sulfide to constrain sulfur sources and the isotopic systematics of precipitation in seafloor hydrothermal vents. Areas studied include the Eastern Manus Basin and Lau Basin back-arc spreading centers and the unsedimented basalt-hosted Southern East Pacific Rise (SEPR) and sediment-hosted Guaymas Basin mid-ocean ridge spreading centers. Chalcopyrite and dissolved hydrogen sulfide (H2S) $δ$34S values range from -5.5{\textperthousand} to +5.6{\textperthousand} in Manus Basin samples, +2.4{\textperthousand} to +6.1{\textperthousand} in Lau Basin samples, and +3.7{\textperthousand} to +5.7{\textperthousand} in SEPR samples. Values of $δ$34S for cubic cubanite and H2S range from -1.4{\textperthousand} to +4.7{\textperthousand} in Guaymas Basin samples. Multiple sulfur isotope systematics in fluid-mineral pairs from the SEPR and Lau Basin show that crustal host rock and thermochemical reduction of seawater-derived dissolved sulfate (SO4) are the primary sources of sulfur in mid-ocean ridge and some back-arc systems. At PACMANUS and SuSu Knolls hydrothermal systems in the Eastern Manus Basin, a significant contribution of sulfur is derived from disproportionation of magmatic sulfur dioxide (SO2), while the remaining sulfur is derived from crustal host rocks and SO4 reduction. At the sedimented Guaymas Basin hydrothermal system, sulfur sources include crustal host rock, reduced seawater SO4, and biogenic sulfide. Vent fluid flow through fresher, less-mature sediment supplies an increased quantity of reactant organic compounds that may reduce 34S-enriched SO4, while fluid interaction with more highly-altered sediments results in H2S characterized by a small, but isotopically-significant input of 34S-depleted biogenic sulfides. Near-zero $Δ$33S values in all samples implicate the abiotic processes of SO4 reduction and leaching of host rock as the major contributors to sulfur content at a high temperature unsedimented mid-ocean ridge and at a back-arc system. $Δ$33S values indicate that SO2 disproportionation is an additional process that contributes sulfur to a different back-arc system and to acid spring-type hydrothermal fluid circulation. At the sedimented Guaymus Basin, near-zero $Δ$33S values are also observed, despite negative $δ$34S values that indicate inputs of biogenic pyrite for some samples. In contrast with previous studies reporting isotope disequilibrium between H2S and chalcopyrite, the $δ$34S values of chalcopyrite sampled from the inner 1{\textendash}2 mm of a chimney wall are within {\textpm}1{\textperthousand} of $δ$34S values for H2S in the paired vent fluid, suggesting equilibrium fluid-mineral sulfur isotope exchange at 300{\textendash}400 {\textdegree}C. Isotopic equilibrium between hydrothermal fluid H2S and precipitating chalcopyrite implies that sulfur isotopes in the chalcopyrite lining across a chimney wall may accurately record past hydrothermal activity.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.gca.2015.02.016},
	author = {McDermott, J M and Ono, S and Tivey, M K and Seewald, J S and Shanks, W C}
}
@article {Kormas2015,
	title = {{Inter-comparison of the potentially active prokaryotic communities in the halocline sediments of Mediterranean deep-sea hypersaline basins}},
	journal = {Extremophiles},
	volume = {19},
	year = {2015},
	pages = {949{\textendash}960},
	abstract = {The sediment microbiota of the Mediterranean deep-sea anoxic hypersaline basins (DHABs) are understudied relative to communities in the brines and halocline waters. In this study, the active fraction of the prokaryotic community in the halocline sediments of L{\textquoteright} Atalante, Urania, and Discovery DHABs was investigated based on extracted total RNA and 454 pyrosequencing of the 16S rRNA gene. Bacterial and archaeal communities were different in the sediments underlying the halocline waters of the three habitats, reflecting the unique chemical settings of each basin. The relative abundance of unique operational taxonomic units (OTUs) was also different between deep-sea control sediments and sediments underlying DHAB haloclines, suggesting adaptation to the steep DHAB chemical gradients. Only a few OTUs were affiliated to known bacterial halophilic and/or anaerobic groups. Many OTUs, including some of the dominant ones, were related to aerobic taxa. Archaea were detected only in few halocline samples, with lower OTU richness relative to Bacteria, and were dominated by taxa associated with methane cycling. This study suggests that, while metabolically active prokaryotic communities appear to be present in sediments underlying the three DHABs investigated, their diversity and activity are likely to be more reduced in sediments underlying the brines.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	author = {Kormas, K and Pachiadaki, M and Karayanni, H and Leadbetter, E R and Bernhard, J M and Edgcomb, V P}
}
@article {ISI:000352192100007,
	title = {{Investigation of extractable organic compounds in deep-sea hydrothermal vent fluids along the Mid-Atlantic Ridge}},
	journal = {GEOCHIMICA ET COSMOCHIMICA ACTA},
	volume = {156},
	year = {2015},
	month = {may},
	pages = {122{\textendash}144},
	type = {Article},
	abstract = {The possibility that deep-sea hydrothermal vents may contain organic compounds produced by abiotic synthesis or by microbial communities living deep beneath the surface has led to numerous studies of the organic composition of vent fluids. Most of these studies have focused on methane and other light hydrocarbons, while the possible occurrence of more complex organic compounds in the fluids has remained largely unstudied. To address this issue, the presence of higher molecular weight organic compounds in deep-sea hydrothermal fluids was assessed at three sites along the Mid-Atlantic Ridge that span a range of temperatures (51 to {\textgreater}360 degrees C), fluid compositions, and host-rock lithologies (mafic to ultramafic). Samples were obtained at several sites within the Lucky Strike, Rainbow, and Lost City hydrothermal fields. Three methods were employed to extract organic compounds for analysis, including liquid: liquid extraction, cold trapping on the walls of a coil of titanium tubing, and pumping fluids through cartridges filled with solid phase extraction (SPE) sorbents. The only samples to consistently yield high amounts of extractable organic compounds were the warm (51-91 degrees C), highly alkaline fluids from Lost City, which contained elevated concentrations of C-8, C-10, and C-12 n-alkanoic acids and, in some cases, trithiolane, hexadecanol, squalene, and cholesterol. Collectively, the C-8-C-12 acids can account for about 15{\%} of the total dissolved organic carbon in the Lost City fluids. The even-carbon-number predominance of the alkanoic acids indicates a biological origin, but it is unclear whether these compounds are derived from microbial activity occurring within the hydrothermal chimney proximal to the site of fluid discharge or are transported from deeper within the system. Hydrothermal fluids from the Lucky Strike and Rainbow fields were characterized by an overall scarcity of extractable dissolved organic compounds. Trace amounts of aromatic hydrocarbons including phenanthrenes and benzothiophene were the only compounds that could be identified as indigenous components of these fluids. Although hydrocarbons and fatty acids were observed in some samples, those compounds were likely derived from particulate matter or biomass entrained during fluid collection. In addition, extracts of some fluid samples from the Rainbow field were found to contain an unresolved complex mixture (UCM) of organic compounds. This UCM shared some characteristics with organic matter extracted from bottom seawater, suggesting that the organic matter observed in these samples might represent seawater-derived compounds that had persisted, albeit with partial alteration, during circulation through the hydrothermal system. While there is considerable evidence that Rainbow and Lost City vent fluids contain methane and other light hydrocarbons produced through abiotic reduction of inorganic carbon, we found no evidence for more complex organic compounds with an abiotic origin in the same fluids. (C) 2015 Elsevier Ltd. All rights reserved.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0016-7037},
	doi = {10.1016/j.gca.2015.02.022},
	author = {McCollom, Thomas M and Seewald, Jeffrey S and German, Christopher R}
}
@article {ISI:000351847600028,
	title = {{Long-term explosive degassing and debris flow activity at West Mata submarine volcano}},
	journal = {GEOPHYSICAL RESEARCH LETTERS},
	volume = {42},
	number = {5},
	year = {2015},
	month = {mar},
	pages = {1480{\textendash}1487},
	type = {Article},
	abstract = {West Mata is a 1200m deep submarine volcano where explosive boninite eruptions were observed in 2009. The acoustic signatures from the volcano{\textquoteright}s summit eruptive vents Hades and Prometheus were recorded with an in situ (similar to 25m range) hydrophone during ROV dives in May 2009 and with local (similar to 5km range) moored hydrophones between December 2009 and August 2011. The sensors recorded low frequency (1-40Hz), short duration explosions consistent with magma bubble bursts from Hades, and broadband, 1-5min duration signals associated with episodes of fragmentation degassing from Prometheus. Long-term eruptive degassing signals, recorded through May 2010, preceded a several month period of declining activity. Degassing episodes were not recorded acoustically after early 2011, although quieter effusive eruption activity may have continued. Synchronous optical measurements of turbidity made between December 2009 and April 2010 indicate that turbidity maxima resulted from occasional south flank slope failures triggered by the collapse of accumulated debris during eruption intervals.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0094-8276},
	doi = {10.1002/2014GL062603},
	author = {Dziak, R P and Bohnenstiehl, D R and Baker, E T and Matsumoto, H and Caplan-Auerbach, J and Embley, R W and Merle, S G and Walker, S L and Lau, T-K. and Chadwick Jr., W W}
}
@article {Germanovich2015,
	title = {{Measuring fluid flow and heat output in seafloor hydrothermal environments}},
	journal = {Journal of Geophysical Research: Solid Earth},
	volume = {120},
	year = {2015},
	pages = {8031{\textendash}8055},
	abstract = {We review techniques for measuring fluid flow and advective heat output from seafloor hydrothermal systems and describe new anemometer and turbine flowmeter devices we have designed, built, calibrated, and tested. These devices allow measuring fluid velocity at high-and low-temperature focused and diffuse discharge sites at oceanic spreading centers. The devices perform at ocean floor depths and black smoker temperatures and can be used to measure flow rates ranging over 2 orders of magnitude. Flow velocity is determined from the rotation rate of the rotor blades or paddle assembly. These devices have an open bearing design that eliminates clogging by particles or chemical precipitates as the fluid passes by the rotors. The devices are compact and lightweight enough for deployment from either an occupied or remotely operated submersible. The measured flow rates can be used in conjunction with vent temperature or geochemical measurements to obtain heat outputs or geochemical fluxes from both vent chimneys and diffuse flow regions. The devices have been tested on 30 Alvin dives on the Juan de Fuca Ridge and 3 Jason dives on the East Pacific Rise (EPR). We measured an anomalously low entrainment coefficient (0.064) and report 104 new measurements over a wide range of discharge temperatures (5 degrees-363 degrees C), velocities (2-199 cm/s), and depths (1517-2511 m). These include the first advective heat output measurements at the High Rise vent field and the first direct fluid flow measurement at Middle Valley. Our data suggest that black smoker heat output at the Main Endeavour vent field may have declined since 1994 and that after the 2005-2006 eruption, the high-temperature advective flow at the EPR 9 degrees 50{\textquoteright}N field may have become more channelized, predominately discharging through the Bio 9 structure. We also report 16 measurements on 10 Alvin dives and 2 Jason dives with flow meters that predate devices described in this work and were used in the process of their development. This includes the first advective measurements in the Lau Basin and at the EPR 9 degrees 39.5{\textquoteright}N. We discuss potential error sources and how they may affect the accuracy of measurements by our devices and other devices. In particular, we use the turbulent plume theory to evaluate the effect of entrainment of ambient seawater.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1002/2015JB012245},
	author = {Germanovich, L N and Hurt, R S and Smith, J E and Genc, G and Lowell, R P}
}
@article {ISI:000366346800001,
	title = {{Metazoans of redoxcline sediments in Mediterranean deep-sea hypersaline anoxic basins}},
	journal = {BMC BIOLOGY},
	volume = {13},
	year = {2015},
	month = {dec},
	type = {Article},
	abstract = {Background: The deep-sea hypersaline anoxic basins (DHABs) of the Mediterranean (water depth similar to 3500 m) are some of the most extreme oceanic habitats known. Brines of DHABs are nearly saturated with salt, leading many to suspect they are uninhabitable for eukaryotes. While diverse bacterial and protistan communities are reported from some DHAB haloclines and brines, loriciferans are the only metazoan reported to inhabit the anoxic DHAB brines. Our goal was to further investigate metazoan communities in DHAB haloclines and brines. Results: We report observations from sediments of three DHAB (Urania, Discovery, L{\textquoteright}Atalante) haloclines, comparing these to observations from sediments underlying normoxic waters of typical Mediterranean salinity. Due to technical difficulties, sampling of the brines was not possible. Morphotype analysis indicates nematodes are the most abundant taxon; crustaceans, loriciferans and bryozoans were also noted. Among nematodes, Daptonema was the most abundant genus; three morphotypes were noted with a degree of endemicity. The majority of rRNA sequences were from planktonic taxa, suggesting that at least some individual metazoans were preserved and inactive. Nematode abundance data, in some cases determined from direct counts of sediments incubated in situ with CellTracker (TM) Green, was patchy but generally indicates the highest abundances in either normoxic control samples or in upper halocline samples; nematodes were absent or very rare in lower halocline samples. Ultrastructural analysis indicates the nematodes in L{\textquoteright}Atalante normoxic control sediments were fit, while specimens from L{\textquoteright}Atalante upper halocline were healthy or had only recently died and those from the lower halocline had no identifiable organelles. Loriciferans, which were only rarely encountered, were found in both normoxic control samples as well as in Discovery and L{\textquoteright}Atalante haloclines. It is not clear how a metazoan taxon could remain viable under this wide range of conditions. Conclusions: We document a community of living nematodes in normoxic, normal saline deep-sea Mediterranean sediments and in the upper halocline portions of the DHABs. Occurrences of nematodes in mid-halocline and lower halocline samples did not provide compelling evidence of a living community in those zones. The possibility of a viable metazoan community in brines of DHABs is not supported by our data at this time.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1741-7007},
	doi = {10.1186/s12915-015-0213-6},
	author = {Bernhard, Joan M and Morrison, Colin R and Pape, Ellen and Beaudoin, David J and Todaro, M Antonio and Pachiadaki, Maria G and Kormas, Konstantinos Ar and Edgcomb, Virginia P}
}
@article {ISI:000367001600001,
	title = {{Microbial Communities on Seafloor Basalts at Dorado Outcrop Reflect Level of Alteration and Highlight Global Lithic Clades}},
	journal = {FRONTIERS IN MICROBIOLOGY},
	volume = {6},
	year = {2015},
	month = {dec},
	type = {Article},
	abstract = {Areas of exposed basalt along mid-ocean ridges and at seafloor outcrops serve as conduits of fluid flux into and out of a subsurface ocean, and microbe-mineral interactions can influence alteration reactions at the rock-water interface. Located on the eastern flank of the East Pacific Rise, Dorado Outcrop is a site of low temperature ({\textless} 20 degrees C) hydrothermal venting and represents a new end-member in the current survey of seafloor basalt biomes. Consistent with prior studies, a survey of 16S rRNA gene sequence diversity using universal primers targeting the V4 hypervariable region revealed much greater richness and diversity on the seafloor rocks than in surrounding seawater. Overall, Gamma-, Alpha-, and Deltaproteobacteria, and Thaumarchaeota dominated the sequenced communities, together making up over half of the observed diversity, though bacterial sequences were more abundant than archaeal in all samples. The most abundant bacterial reads were closely related to the obligate chemolithoautotrophic, sulfur-oxidizing Thioprofundum lifl-lotrophicum, suggesting carbon and sulfur cycling as dominant metabolic pathways in this system. Representatives of Thaumarchaeota were detected in relatively high abundance on the basalts in comparison to bottom water, possibly indicating ammonia oxidation. In comparison to other sequence datasets from globally distributed seafloor basalts, this study reveals many overlapping and cosmopolitan phylogenetic groups and also suggests that substrate age correlates with community structure.},
	keywords = {AUV Sentry (Autonomous Underwater Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {1664-302X},
	doi = {10.3389/fmicb.2015.01470},
	author = {Lee, Michael D and Walworth, Nathan G and Sylvan, Jason B and Edwards, Katrina J and Orcutt, Beth N}
}
@article {Scott2015,
	title = {{Microbial Iron Mats at the Mid-Atlantic Ridge and Evidence that Zetaproteobacteria May Be Restricted to Iron-Oxidizing Marine Systems}},
	journal = {PLoS ONE},
	volume = {10},
	number = {3},
	year = {2015},
	pages = {e0119284},
	abstract = {Chemolithoautotrophic iron-oxidizing bacteria play an essential role in the global iron cycle. Thus far, the majority of marine iron-oxidizing bacteria have been identified as Zetaproteobacteria, a novel class within the phylum Proteobacteria. Marine iron-oxidizing microbial communities have been found associated with volcanically active seamounts, crustal spreading centers, and coastal waters. However, little is known about the presence and diversity of iron-oxidizing communities at hydrothermal systems along the slow crustal spreading center of the Mid-Atlantic Ridge. From October to November 2012, samples were collected from rust-colored mats at three well-known hydrothermal vent systems on the Mid-Atlantic Ridge (Rainbow, Trans-Atlantic Geotraverse, and Snake Pit) using the ROV Jason II. The goal of these efforts was to determine if iron-oxidizing Zetaproteobacteria were present at sites proximal to black smoker vent fields. Small, diffuse flow venting areas with high iron(II) concentrations and rust-colored microbial mats were observed at all three sites proximal to black smoker chimneys. A novel, syringe-based precision sampler was used to collect discrete microbial iron mat samples at the three sites. The presence of Zetaproteobacteria was confirmed using a combination of 16S rRNA pyrosequencing and single-cell sorting, while light micros-copy revealed a variety of iron-oxyhydroxide structures, indicating that active iron-oxidizing communities exist along the Mid-Atlantic Ridge. Sequencing analysis suggests that these iron mats contain cosmopolitan representatives of Zetaproteobacteria, but also exhibit diversity that may be uncommon at other iron-rich marine sites studied to date. A meta-analysis of publically available data encompassing a variety of aquatic habitats indicates that Zetaproteobacteria are rare if an iron source is not readily available. This work adds to the growing understanding of Zetaproteobacteria ecology and suggests that this organism is likely locally restricted to iron-rich marine environments but may exhibit wide-scale geographic distribution, further underscoring the importance of Zetaproteobacteria in global iron cycling.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1371/journal.pone.0119284},
	author = {Scott, J J and Breier, J A and Luther, G W and Emerson, D}
}
@article {Faak2015,
	title = {{Near conductive cooling rates in the upper-plutonic section of crust formed at the East Pacific Rise}},
	journal = {Earth and Planetary Science Letters},
	volume = {423},
	year = {2015},
	pages = {36{\textendash}47},
	abstract = {A new geospeedometer, based on diffusion modeling of Mg in plagioclase, is used to determine cooling rates of the upper section of the lower oceanic crust formed at fast-spreading mid-ocean ridges. The investigated natural sample suites include gabbroic rocks formed at three different locations along the fast-spreading East Pacific Rise. These samples cover a depth interval of 0{\textendash}840 m below the sheeted dike/gabbro boundary and therefore allow the variation of cooling rate as a function of depth within the upper plutonic sequence to be determined. We demonstrate that the cooling rates we obtained are robust (reproducible and consistent across different vertical sections at fast spreading ridges) and decrease significantly with increasing sample depth (covering almost 4 orders of magnitude, ranging from \~{}1 {\textdegree}C y-1 for the shallowest samples to 0.0003 {\textdegree}C y-1 for the deepest samples). Both the absolute cooling rates, and the rate of decrease of cooling rate with depth, are consistent with conductive thermal models. In contrast, the absolute cooling rates determined from the deeper samples ({\textgreater}300 m below DGB), and the large decrease in cooling rate with depth are inconsistent with thermal models that include substantial cooling by off-axis hydrothermal circulation within the upper plutonic section of the crust.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.epsl.2015.04.025},
	author = {Faak, K and Coogan, L A and Chakraborty, S}
}
@article {Bemis2015,
	title = {{The path to COVIS: A review of acoustic imaging of hydrothermal flow regimes}},
	journal = {Deep-Sea Research Part II: Topical Studies in Oceanography},
	volume = {121},
	year = {2015},
	pages = {159{\textendash}176},
	abstract = {Acoustic imaging of hydrothermal flow regimes started with the incidental recognition of a plume on a routine sonar scan for obstacles in the path of the human-occupied submersible ALVIN. Developments in sonar engineering, acoustic data processing and scientific visualization have been combined to develop technology which can effectively capture the behavior of focused and diffuse hydrothermal discharge. This paper traces the development of these acoustic imaging techniques for hydrothermal flow regimes from their conception through to the development of the Cabled Observatory Vent Imaging Sonar (COVIS). COVIS has monitored such flow eight times a day for several years. Successful acoustic techniques for estimating plume entrainment, bending, vertical rise, volume flux, and heat flux are presented as is the state-of-the-art in diffuse flow detection.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.dsr2.2015.06.002},
	author = {Bemis, K G and Silver, D and Xu, G and Light, R and Jackson, D R and Jones, C D and Ozer, S and Liu, L}
}
@article {ISI:000356731300054,
	title = {{Pathways for abiotic organic synthesis at submarine hydrothermal fields}},
	journal = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
	volume = {112},
	number = {25},
	year = {2015},
	month = {jun},
	pages = {7668{\textendash}7672},
	type = {Article},
	abstract = {Arguments for an abiotic origin of low-molecular weight organic compounds in deep-sea hot springs are compelling owing to implications for the sustenance of deep biosphere microbial communities and their potential role in the origin of life. Theory predicts that warm H-2-rich fluids, like those emanating from serpentinizing hydrothermal systems, create a favorable thermodynamic drive for the abiotic generation of organic compounds from inorganic precursors. Here, we constrain two distinct reaction pathways for abiotic organic synthesis in the natural environment at the Von Damm hydrothermal field and delineate spatially where inorganic carbon is converted into bioavailable reduced carbon. We reveal that carbon transformation reactions in a single system can progress over hours, days, and up to thousands of years. Previous studies have suggested that CH4 and higher hydrocarbons in ultramafic hydrothermal systems were dependent on H-2 generation during active serpentinization. Rather, our results indicate that CH4 found in vent fluids is formed in H-2-rich fluid inclusions, and higher n-alkanes may likely be derived from the same source. This finding implies that, in contrast with current paradigms, these compounds may form independently of actively circulating serpentinizing fluids in ultramafic-influenced systems. Conversely, widespread production of formate by SCO2 reduction at Von Damm occurs rapidly during shallow subsurface mixing of the same fluids, which may support anaerobic methanogenesis. Our finding of abiogenic formate in deep-sea hot springs has significant implications for microbial life strategies in the present- day deep biosphere as well as early life on Earth and beyond.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0027-8424},
	doi = {10.1073/pnas.1506295112},
	author = {McDermott, Jill M and Seewald, Jeffrey S and German, Christopher R and Sylva, Sean P}
}
@article {ISI:000350053000004,
	title = {{Phylogenetic placement of Cibicidoides wuellerstorfi (Schwager, 1866) from methane seeps and non-seep habitats on the Pacific margin}},
	journal = {GEOBIOLOGY},
	volume = {13},
	number = {1},
	year = {2015},
	month = {jan},
	pages = {44{\textendash}52},
	type = {Article},
	abstract = {Benthic foraminifera are among the most abundant groups found in deep-sea habitats, including methane seep environments. Unlike many groups, no endemic foraminiferal species have been reported from methane seeps, and to our knowledge, genetic data are currently sparse for Pacific deep-sea foraminifera. In an effort to understand the relationships between seep and non-seep populations of the deep-sea foraminifera Cibicidoides wuellerstorfi, a common paleo-indicator species, specimens from methane seeps in the Pacific were analyzed and compared to one another for genetic similarities of small subunit rDNA (SSU rDNA) sequences. Pacific Ocean C. wuellerstorfi were also compared to those collected from other localities around the world (based on 18S gene available on Genbank, e.g., Schweizer et al., 2009). Results from this study revealed that C. wuellerstorfi living in seeps near Costa Rica and Hydrate Ridge are genetically similar to one another at the species level. Individuals collected from the same location that display opposite coiling directions (dextral and sinstral) had no species level genetic differences. Comparisons of specimens with genetic information available from Genbank (SSU rDNA) showed that Pacific individuals, collected for this study, are genetically similar to those previously analyzed from the North Atlantic and Antarctic. These observations provide strong evidence for the true cosmopolitan nature of C. wuellerstorfi and highlight the importance of understanding how these microscopic organisms are able to maintain sufficient genetic exchange to remain within the same species between seep and non-seep habitats and over global distances.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1472-4677},
	doi = {10.1111/gbi.12118},
	author = {Burkett, A M and Rathburn, A E and Perez, M E and Levin, L A and Cha, H and Rouse, G W}
}
@article {ISI:000358260100015,
	title = {{Predicting the response of the deep-ocean microbiome to geochemical perturbations by hydrothermal vents}},
	journal = {ISME JOURNAL},
	volume = {9},
	number = {8},
	year = {2015},
	month = {aug},
	pages = {1857{\textendash}1869},
	type = {Article},
	abstract = {Submarine hydrothermal vents perturb the deep-ocean microbiome by injecting reduced chemical species into the water column that act as an energy source for chemosynthetic organisms. These systems thus provide excellent natural laboratories for studying the response of microbial communities to shifts in marine geochemistry. The present study explores the processes that regulate coupled microbial-geochemical dynamics in hydrothermal plumes by means of a novel mathematical model, which combines thermodynamics, growth and reaction kinetics, and transport processes derived from a fluid dynamics model. Simulations of a plume located in the ABE vent field of the Lau basin were able to reproduce metagenomic observations well and demonstrated that the magnitude of primary production and rate of autotrophic growth are largely regulated by the energetics of metabolisms and the availability of electron donors, as opposed to kinetic parameters. Ambient seawater was the dominant source of microbes to the plume and sulphur oxidisers constituted almost 90{\%} of the modelled community in the neutrally-buoyant plume. Data from drifters deployed in the region allowed the different time scales of metabolisms to be cast in a spatial context, which demonstrated spatial succession in the microbial community. While growth was shown to occur over distances of tens of kilometers, microbes persisted over hundreds of kilometers. Given that high-temperature hydrothermal systems are found less than 100 km apart on average, plumes may act as important vectors between different vent fields and other environments that are hospitable to similar organisms, such as oil spills and oxygen minimum zones.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1751-7362},
	doi = {10.1038/ismej.2015.4},
	author = {Reed, Daniel C and Breier, John A and Jiang, Houshuo and Anantharaman, Karthik and Klausmeier, Christopher A and Toner, Brandy M and Hancock, Cathrine and Speer, Kevin and Thurnherr, Andreas M and Dick, Gregory J}
}
@article {ISI:000353740700016,
	title = {{Rapid assessment of fish communities on submerged oil and gas platform reefs using remotely operated vehicles}},
	journal = {FISHERIES RESEARCH},
	volume = {167},
	year = {2015},
	month = {jul},
	pages = {143{\textendash}155},
	type = {Article},
	abstract = {Remotely operated vehicles (ROVs) provide a non-extractive approach to characterizing fish communities in complex habitats. Despite the demonstrated effectiveness of ROVs in studying reef fishes over natural hard-bottom and small artificial reefs, there has been little application of this technology to larger artificial structures (10s of m tall and wide), We explored the utility of ROVs in rapidly characterizing an assemblage of fishes associated with an artificial reef complex in the western Gulf of Mexico (26.9-28.2 degrees N; 95.5-97.0 degrees W) dominated by partially removed and toppled oil and gas platforms. This study reports on an efficient method to sample these structures, where we integrated depth-interval transect (DIT) and continuous roving transect (CRT) protocols to document fish distribution and community structure on 14 artificial reef sites. Consistent with previous hydroacoustic studies, south Texas artificial reefs exhibited a vertically heterogeneous distribution of fishes that varied with structure orientation. These reefs were dominated by economically important lutjanids and carangids, both of which presented sampling challenges due to their patchy distribution around these vast structures. The non-uniform distribution and mobility of these dominant taxa highlight the utility of adopting roving approaches to assess fish communities on these complex structures. We conclude our study with a discussion of important logistical challenges associated with micro-ROV surveys in deepwater habitats, and potential complementary approaches to assist documentation of demersal fishes inhabiting a persistently turbid bottom layer. (C) 2015 Elsevier B.V. All rights reserved.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0165-7836},
	doi = {10.1016/j.fishres.2015.02.011},
	author = {Ajemian, Matthew J and Wetz, Jennifer Jarrell and Shipley-Lozano, Brooke and Stunz, Gregory W}
}
@article {ISI:000354588900002,
	title = {{Sources of organic carbon for Rimicaris hybisae: Tracing individual fatty acids at two hydrothermal vent fields in the Mid-Cayman rise}},
	journal = {DEEP-SEA RESEARCH PART I-OCEANOGRAPHIC RESEARCH PAPERS},
	volume = {100},
	year = {2015},
	month = {jun},
	pages = {13{\textendash}20},
	type = {Article},
	abstract = {Hydrothermal vents harbor ecosystems mostly decoupled from organic carbon synthesized with the energy of sunlight (photosynthetic carbon source) but fueled instead by oxidation of reduced compounds to generate a chemosynthetic carbon source. Our study aimed to disentangle photosynthetic and chemosynthetic organic carbon sources for the shrimp species Rimicaris hybisae, a primary consumer presumed to obtain its organic carbon mainly from ectosymbiotic chemoautotrophic bacteria living on its gill cover membrane. To provide ectosymbionts with ideal conditions for chemosynthesis, these shrimp live in dense clusters around vent chimneys; they are, however, also found sparsely distributed adjacent to diffuse vent flows, where they might depend on alternative food sources. Densely and sparsely distributed shrimp were sampled and dissected into abdominal tissue and gill cover membrane, covered with ectosymbiotic bacteria, at two hydrothermal vent fields in the Mid-Cayman rise that differ in vent chemistry. Fatty acids (FA) were extracted from shrimp tissues and their carbon isotopic compositions assessed. The FA data indicate that adult R. hybisae predominantly rely on bacteria for their organic carbon needs. Their FA composition is dominated by common bacterial FA of the n7 family (similar to 41{\%}). Bacterial FA of the n4 FA family are also abundant and found to constitute good biomarkers for gill ectosymbionts. Sparsely distributed shrimp contain fractions of n4 FA in gill cover membranes similar to 4{\%} lower than densely packed ones (similar to 18{\%}) and much higher fractions of photosynthetic FA in abdominal tissues, similar to 4{\%} more (compared with 1.6{\%}), suggesting replacement of ectosymbionts along with exoskeletons (molt), while they take up alternative diets of partly photosynthetic organic carbon. Abdominal tissues also contain photosynthetic FA from a second source taken up presumably during an early dispersal phase and still present to c. 3{\%} in adult shrimp. The contribution of photosynthetic carbon to the FA pool of adult R. hybisae is, however, overall small (max. 8{\%}). Significant differences in carbon isotopic values of chemosynthetically derived FA between vent fields suggest that different dominant C fixation pathways are being used. (C) 2015 Published by Elsevier Ltd.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0637},
	doi = {10.1016/j.dsr.2015.02.003},
	author = {Streit, Kathrin and Bennett, Sarah A and Van Dover, Cindy L and Coleman, Max}
}
@article {Lee2015,
	title = {{Temporal and spatial variation in temperature experienced by macrofauna at Main Endeavour hydrothermal vent field}},
	journal = {Deep-Sea Research Part I: Oceanographic Research Papers},
	volume = {106},
	year = {2015},
	pages = {154{\textendash}166},
	abstract = {A significant focus of hydrothermal vent ecological studies has been to understand how species cope with various stressors through physiological tolerance and biochemical resistance. Yet, the environmental conditions experienced by vent species have not been well characterized. This objective requires continuous observations over time intervals that can capture environmental variability at scales that are relevant to animals. We used autonomous temperature logger arrays (four roughly parallel linear arrays of 12 loggers spaced every 10{\textendash}12 cm) to study spatial and temporal variations in the thermal regime experienced by hydrothermal vent macrofauna at a diffuse flow vent. Hourly temperatures were recorded over eight months from 2010 to 2011 at Grotto vent in the Main Endeavour vent field on the Juan de Fuca Ridge, a focus area of the Ocean Networks Canada cabled observatory. The conspicuous animal assemblages in video footage contained Ridgeia piscesae tubeworms, gastropods (primarily Lepetodrilus fucensis), and polychaetes (polynoid scaleworms and the palm worm Paralvinella palmiformis). Two dimensional spatial gradients in temperature were generally stable over the deployment period. The average temperature recorded by all arrays, and in some individual loggers, revealed distinctive fluctuations in temperature that often corresponded with the tidal cycle. We postulate that this may be related to changes in bottom currents or fluctuations in vent discharge. A marked transient temperature increase lasting over a period of days was observed in April 2011. While the distributions and behavior of Juan de Fuca Ridge vent invertebrates may be partially constrained by environmental temperature and temperature tolerance, except for the one transient high-temperature event, observed fluid temperatures were generally similar to the thermal preferences for some species, and typically well below lethal temperatures for all species. Average temperatures of the four arrays ranged from 4.1 to 11.0 {\textdegree}C during the deployment, indicating that on an hourly timescale the temperature conditions in this tubeworm community were fairly moderate and stable. The generality of these findings and behavioral responses of vent organisms to predictable},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.dsr.2015.10.004},
	author = {Lee, R W and Robert, K and Matabos, M and Bates, A E and Juniper, S K}
}
@article {Quattrini2015,
	title = {{Testing the depth-differentiation hypothesis in a deepwater octocoral}},
	journal = {Proceedings of the Royal Society B: Biological Sciences},
	volume = {282},
	number = {1807},
	year = {2015},
	pages = {20150008},
	abstract = {The depth-differentiation hypothesis proposes that the bathyal region is a source of genetic diversity and an area where there is a high rate of species formation. Genetic differentiation should thus occur over relatively small vertical distances, particularly along the upper continental slope (200-1000 m) where oceanography varies greatly over small differences in depth. To test whether genetic differentiation within deepwater octocorals is greater over vertical rather than geographical distances, Callogorgia delta was targeted. This species commonly occurs throughout the northern Gulf of Mexico at depths ranging from 400 to 900 m. We found significant genetic differentiation (FST = 0.042) across seven sites spanning 400 km of distance and 400 m of depth. A pattern of isolation by depth emerged, but geographical distance between sites may further limit gene flow. Water mass boundaries may serve to isolate populations across depth; however, adaptive divergence with depth is also a possible scenario. Microsatellite markers also revealed significant genetic differentiation (FST = 0.434) between C. delta and a closely related species, Callogorgia americana, demonstrating the utility of microsatellites in species delimitation of octocorals. Results provided support for the depth-differentiation hypothesis, strengthening the notion that factors covarying with depth serve as isolation mechanisms in deep-sea populations.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1098/rspb.2015.0008},
	author = {Quattrini, A M and Baums, I B and Shank, T M and Morrison, C and Cordes, E E}
}
@article {Findlay2015,
	title = {{Trace metal concentration and partitioning in the first 1.5 m of hydrothermal vent plumes along the Mid-Atlantic Ridge: TAG, Snakepit, and Rainbow}},
	journal = {Chemical Geology},
	volume = {412},
	year = {2015},
	pages = {117{\textendash}131},
	abstract = {To determine the significance of metal fluxes from hydrothermal vents, understanding the speciation, reactivity, and possible transformations of metals and metal sulfides within the hydrothermal plume is critical. In this study, we measure the concentration and partitioning of trace metals (Fe, Mn, Cu, Cd, Co, Pb, Ni) and sulfide phases within the first 1.5 m of the rising plume at three vent fields (TAG, Snakepit, and Rainbow) along the Mid-Atlantic Ridge. A HCl/HNO3 leaching method was used to differentiate metals present in metal mono-sulfides from those in pyrite and chalcopyrite. At all three vent sites, Mn and Fe are primarily in the {\textless} 0.2 $μ$m (filtered) portion, whereas Cu, Co, Cd, and Pb are mainly in the unfiltered fraction. Significant concentrations of HNO3-extractable metals were found in the {\textless} 0.2 $μ$m fraction at all three vent sites, indicating that they likely exist in a recalcitrant nanoparticulate phase such as pyrite or chalcopyrite. At TAG and Snakepit, Cu is correlated with Co, as Co enters into chalcopyrite and other CuFeS phases and Zn is correlated with Cd and Pb as they form discrete metal sulfide phases. At Rainbow, Zn, Cd, and Pb are correlated, but Cu and Co are not correlated. The Rainbow data are consistent with the higher metal to sulfide ratio found at Rainbow. These speciation differences are significant as both mineral type and size will affect the amount of metal transported from the vent site and its availability for biogeochemical processes.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.chemgeo.2015.07.021},
	author = {Findlay, A J and Gartman, A and Shaw, T J and Luther, G W}
}
@article {ISI:000330965000004,
	title = {{Age and growth of the cold-water scleractinian Solenosmilia variabilis and its reef on SW Pacific seamounts}},
	journal = {CORAL REEFS},
	volume = {33},
	number = {1},
	year = {2014},
	month = {mar},
	pages = {31{\textendash}38},
	type = {Article},
	abstract = {Little is known about growth rates of deep-water reef-forming corals or the rates at which these reefs accumulate. Such information is critical for determining the resilience of the reefs to anthropogenic impacts such as trawling and climate change. We radiocarbon date live-caught and sub-fossil samples of the bioherm-forming coral Solenosmilia variabilis collected from precisely known depths and locations by means of a remotely operated vehicle on seamounts south of Tasmania, Australia. The growth rate of colonies live-caught between 958 and 1,454 m, which spans most of the depth range of the species locally, ranged from 0.84 to 1.25 mm linear extension yr(-1) and tended to be higher in the deeper-caught material. Analysis of skeletal microstructure suggests annual deposition of growth increments near the growing tips, but not closer to the base, as the skeleton is extended and thickened. Dating of sub-fossil material indicates S. variabilis has been present on Tasmanian seamounts for at least the last 47,000 yrs and a reef accumulation rate of 0.27 mm yr(-1).},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0722-4028},
	doi = {10.1007/s00338-013-1097-y},
	author = {Fallon, S J and Thresher, R E and Adkins, J}
}
@article {Bernhard2014,
	title = {{Benthic protists and fungi of Mediterranean deep hypsersaline anoxic basin redoxcline sediments}},
	journal = {Frontiers in Microbiology},
	volume = {5},
	year = {2014},
	pages = {605},
	abstract = {Some of the most extreme marine habitats known are the Mediterranean deep hypersaline anoxic basins (DHABs; water depth \~{}3500 m). Brines of DHABs are nearly saturated with salt, leading many to suspect they are uninhabitable for eukaryotes. While diverse bacterial and protistan communities are reported from some DHAB water-column haloclines and brines, the existence and activity of benthic DHAB protists have rarely been explored. Here, we report findings regarding protists and fungi recovered from sediments of three DHAB (Discovery, Urania, L{\textquoteright} Atalante) haloclines, and compare these to communities from sediments underlying normoxic waters of typical Mediterranean salinity. Halocline sediments, where the redoxcline impinges the seafloor, were studied from all three DHABs. Microscopic cell counts suggested that halocline sediments supported denser protist populations than those in adjacent control sediments. Pyrosequencing analysis based on ribosomal RNA detected eukaryotic ribotypes in the halocline sediments from each of the three DHABs, most of which were fungi. Sequences affiliated with Ustilaginomycotina Basidiomycota were the most abundant eukaryotic signatures detected. Benthic communities in these DHABs appeared to differ, as expected, due to differing brine chemistries. Microscopy indicated that only a low proportion of protists appeared to bear associated putative symbionts. In a considerable number of cases, when prokaryotes were associated with a protist, DAPI staining did not reveal presence of any nuclei, suggesting that at least some protists were carcasses inhabited by prokaryotic scavengers.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.3389/fmicb.2014.00605},
	author = {Bernhard, J M and Kormas, K and Pachiadaki, M and Rocke, E and Beaudoin, D J and Morrison, C and Visscher, P T and Cobban, A and Starczak, V R and Edgcomb, V P}
}
@article {Demopoulos2014,
	title = {{Biodiversity and community composition of sediment macrofauna associated with deep-sea Lophelia pertusa habitats in the Gulf of Mexico}},
	journal = {Deep-Sea Research. Part I: Oceanographic Research Papers},
	volume = {93},
	year = {2014},
	pages = {91{\textendash}103},
	abstract = {Scleractinian corals create three-dimensional reefs that provide sheltered refuges, facilitate sediment accumulation, and enhance colonization of encrusting fauna. While heterogeneous coral habitats can harbor high levels of biodiversity, their effect on the community composition within nearby sediments remains unclear, particularly in the deep sea. Sediment macrofauna from deep-sea coral habitats (Lophelia pertusa) and non-coral, background sediments were examined at three sites in the northern Gulf of Mexico (VK826, VK906, MC751, 350{\textendash}500 m depth) to determine whether macrofaunal abundance, diversity, and community composition near corals differed from background soft-sediments. Macrofaunal densities ranged from 26 to 125 individuals 32 cm-2 and were significantly greater near coral versus background sediments only at VK826. Of the 86 benthic invertebrate taxa identified, 16 were exclusive to near-coral habitats, while 14 were found only in background sediments. Diversity (Fisher{\textquoteright}s $\alpha$) and evenness were significantly higher within near-coral sediments only at MC751 while taxon richness was similar among all habitats. Community composition was significantly different both between near-coral and background sediments and among the three primary sites. Polychaetes numerically dominated all samples, accounting for up to 70{\%} of the total individuals near coral, whereas peracarid crustaceans were proportionally more abundant in background sediments (18{\%}) than in those near coral (10{\%}). The reef effect differed among sites, with community patterns potentially influenced by the size of reef habitat. Taxon turnover occurred with distance from the reef, suggesting that reef extent may represent an important factor in structuring sediment communities near L. pertusa. Polychaete communities in both habitats differed from other Gulf of Mexico (GOM) soft sediments based on data from previous studies, and we hypothesize that local environmental conditions found near L. pertusa may influence the macrofaunal community structure beyond the edges of the reef. This study represents the first assessment of L. pertusa-associated sediment communities in the GOM and provides baseline data that can help define the role of transition zones, from deep reefs to soft sediments, in shaping macrofaunal community structure and maintaining biodiversity; this information can help guide future conservation and management activities.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.dsr.2014.07.014},
	author = {Demopoulos, A W J and Bourque, J R and Frometa, J}
}
@article {ISI:000338758400018,
	title = {{THE CASCADIA INITIATIVE A Sea Change In Seismological Studies of Subduction Zones}},
	journal = {OCEANOGRAPHY},
	volume = {27},
	number = {2, SI},
	year = {2014},
	month = {jun},
	pages = {138{\textendash}150},
	type = {Article},
	abstract = {Increasing public awareness that the Cascadia subduction zone in the Pacific Northwest is capable of great earthquakes (magnitude 9 and greater) motivates the Cascadia Initiative, an ambitious onshore/offshore seismic and geodetic experiment that takes advantage of an amphibious array to study questions ranging from megathrust earthquakes, to volcanic arc structure, to the formation, deformation and hydration of the Juan De Fuca and Gorda Plates. Here, we provide an overview of the Cascadia Initiative, including its primary science objectives, its experimental design and implementation, and a preview of how the resulting data are being used by a diverse and growing scientific community. The Cascadia Initiative also exemplifies how new technology and community-based experiments are opening up frontiers for marine science. The new technology shielded ocean bottom seismometers is allowing more routine investigation of the source zone of megathrust earthquakes, which almost exclusively lies offshore and in shallow water. The Cascadia Initiative offers opportunities and accompanying challenges to a rapidly expanding community of those who use ocean bottom seismic data.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1042-8275},
	author = {Toomey, Douglas R and Allen, Richard M and Barclay, Andrew H and Bell, Samuel W and Bromirski, Peter D and Carlson, Richard L and Chen, Xiaowei and Collins, John A and Dziak, Robert P and Evers, Brent and Forsyth, Donald W and Gerstoft, Peter and Hooft, Emilie E E and Livelybrooks, Dean and Lodewyk, Jessica A and Luther, Douglas S and McGuire, Jeffrey J and Schwartz, Susan Y and Tolstoy, Maya and Trehu, Anne M and Weirathmueller, Michelle and Wilcock, William S D}
}
@article {Edgcomb2014,
	title = {{Ciliates along Oxyclines of Permanently Stratified Marine Water Columns}},
	journal = {Journal of Eukaryotic Microbiology},
	volume = {61},
	number = {4},
	year = {2014},
	pages = {434{\textendash}445},
	abstract = {Studies of microbial communities in areas of the world where permanent marine water column oxyclines exist suggest they are "hotspots" of microbial activity, and that these water features and the anoxic waters below them are inhabited by diverse protist taxa, including ciliates. These communities have minimal taxonomic overlap with those in overlying oxic water columns. Some ciliate taxa have been detected in multiple locations where these stable water column oxyclines exist; however, differences in such factors as hydrochemistry in the habitats that have been studied suggest local selection for distinct communities. We compare published data on ciliate communities from studies of deep marine water column oxyclines in Caricao Basin, Venezuela, and the Black Sea, with data from coastal, shallower oxycline waters in Framvaren and Mariager fjords, and from several deep-sea hypersaline anoxic basins in the Eastern Mediterranean Sea. Putative symbioses between Bacteria, Archaea, and ciliates observed along these oxyclines suggests a strategy of cooperative metabolism for survival that includes chemosynthetic autotrophy and exchanges of metabolic intermediates or end products between hosts and their prokaryotic partners.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1111/jeu.12122},
	author = {Edgcomb, V P and Pachiadaki, M}
}
@article {ISI:000338027700011,
	title = {{Depth-dependent gene flow in Gulf of Mexico cold seep Lamellibrachia tubeworms (Annelida, Siboglinidae)}},
	journal = {HYDROBIOLOGIA},
	volume = {736},
	number = {1},
	year = {2014},
	month = {sep},
	pages = {139{\textendash}154},
	type = {Article},
	abstract = {Lamellibrachia vestimentiferan tubeworms form aggregations at hydrocarbon cold seeps in the deep Gulf of Mexico (GoM), creating structures that provide living space for other fauna. In the GoM, three Lamellibrachia taxa vary in morphology and depth ranges: Lamellibrachia luymesi (300-950 m), Lamellibrachia sp. 1 (950-2,604 m), and Lamellibrachia sp. 2 (1,175-3,304 m). While Lamellibrachia sp. 2 is consistently identified as a separate species, L. luymesi and sp. 1 cannot be discriminated using barcoding markers cytochrome oxidase subunit 1 (COI) and large ribosomal subunit rDNA (16S). To determine if limited gene flow was a factor in the formation of these taxa, we employed more quickly evolving markers, including mitochondrial cytochrome B (CYTB), hemoglobin subunit B2 intron (HbB2i), and six polymorphic microsatellites; microsatellites were amplified across 45 L. luymesi and sp. 1 individuals. Additionally, we used microsatellites to ask whether populations of Lamellibrachia sp. 1 and sp. 2 show evidence of significant structure. Despite a lack of resolution seen with CYTB and HbB2i, L. luymesi and sp. 1 form genetically differentiated clusters at the cross-amplified microsatellites. Furthermore, we find no evidence for population structure for either Lamellibrachia sp. 1 or sp. 2 across the GoM.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0018-8158},
	doi = {10.1007/s10750-014-1900-y},
	author = {Cowart, Dominique A and Halanych, Kenneth M and Schaeffer, Stephen W and Fisher, Charles R}
}
@article {ISI:000343625500006,
	title = {{Dissolved hydrogen and methane in the oceanic basaltic biosphere}},
	journal = {EARTH AND PLANETARY SCIENCE LETTERS},
	volume = {405},
	year = {2014},
	month = {nov},
	pages = {62{\textendash}73},
	type = {Article},
	abstract = {The oceanic basaltic crust is the largest aquifer on Earth and has the potential to harbor substantial subsurface microbial ecosystems, which hitherto remains largely uncharacterized and is analogous to extraterrestrial subsurface habitats. Within the sediment-buried 3.5 Myr old basaltic crust of the eastern Juan de Fuca Ridge flank, the circulating basement fluids have moderate temperature (similar to 65 degrees C) and low to undetectable dissolved oxygen and nitrate concentrations. Sulfate, present in high concentrations, is therefore expected to serve as the major electron acceptor in this subsurface environment. This study focused on the availability and potential sources of two important electron donors, methane (CH4) and hydrogen (H-2), for the subseafloor biosphere. High integrity basement fluids were collected via fluid delivery lines associated with Integrated Ocean Drilling Program (IODP) Circulation Obviation Retrofit Kits (CORKs) that extend from basement depths to outlet ports at the seafloor. Two new CORKs installed during IODP 327 in 2010, 1362A and 1362B, were sampled in 2011 and 2013. The two CORKs are superior than earlier style CORKs in that they are equipped with coated casing and polytetrafluoroethylene fluid delivery lines, reducing the interaction between casing materials with the environment. Additional samples were collected from an earlier style CORK at Borehole 1301A. The basement fluids are enriched in H-2 (0.05-1.8 mu mol/kg), suggesting that the ocean basaltic aquifer can support H-2-driven metabolism. The basement fluids also contain significant amount of CH4 (5-32 mu mol/kg), revealing CH4 as an available substrate for subseafloor basaltic habitats. The delta C-13 values of CH4 from the three boreholes ranged from -22.5 to -58 parts per thousand, while the delta H-2 values ranged from 316 to 57 parts per thousand. The isotopic compositions of CH4 and the molecular compositions of hydrocarbons suggest that CH4 in the basement fluids is of both biogenic and abiotic origins, varying among sites and sampling times. The delta H-2 values of CH4 in CORK 1301A fluid samples are much more positive than found in all other marine environments investigated to date and are best explained by the partial microbial oxidation of biogenic CH4. In conclusion, our study shows that CH4 and H-2 are persistently available to fuel the deep biosphere and that CH4 is both produced and potentially consumed by microorganisms in the oceanic basement. (C) 2014 Elsevier B.V. All rights reserved.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0012-821X},
	doi = {10.1016/j.epsl.2014.07.037},
	author = {Lin, Huei-Ting and Cowen, James P and Olson, Eric J and Lilley, Marvin D and Jungbluth, Sean P and Wilson, Samuel T and Rappe, Michael S}
}
@article {ISI:000342622400022,
	title = {{Distribution and size fractionation of elemental sulfur in aqueous environments: The Chesapeake Bay and Mid-Atlantic Ridge}},
	journal = {GEOCHIMICA ET COSMOCHIMICA ACTA},
	volume = {142},
	year = {2014},
	month = {oct},
	pages = {334{\textendash}348},
	type = {Article},
	abstract = {Elemental sulfur is an important intermediate of sulfide oxidation and may be produced via abiotic and biotic pathways. In this study the concentration and size fractionation of elemental sulfur were measured in two different sulfidic marine environments: the Chesapeake Bay and buoyant hydrothermal vent plumes along the Mid-Atlantic Ridge. Nanoparticulate sulfur ({\textless}0.2 mu m) was found to comprise up to 90{\%} of the total elemental sulfur in anoxic deep waters of the Chesapeake Bay. These data were compared with previous studies of elemental sulfur, and represent one of the few reports of nanoparticulate elemental sulfur in the environment. Additionally, a strain of phototrophic sulfide oxidizing bacteria isolated from the Chesapeake Bay was shown to produce elemental sulfur as a product of sulfide oxidation. Elemental sulfur concentrations are also presented from buoyant hydrothermal vent plumes located along the Mid-Atlantic Ridge. In the Mid-Atlantic Ridge plume, S-0 concentrations up to 33 mu M were measured in the first meter of rising plumes at three different vent sites, and nanoparticulate S-0 was up to 44{\%} of total elemental sulfur present. (C) 2014 Elsevier Ltd. All rights reserved.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0016-7037},
	doi = {10.1016/j.gca.2014.07.032},
	author = {Findlay, Alyssa J and Gartman, Amy and MacDonald, Daniel J and Hanson, Thomas E and Shaw, Timothy J and Luther III, George W}
}
@article {ISI:000346944000013,
	title = {{Diverse magma flow directions during construction of sheeted dike complexes at fast- to superfast-spreading centers}},
	journal = {EARTH AND PLANETARY SCIENCE LETTERS},
	volume = {408},
	year = {2014},
	month = {dec},
	pages = {119{\textendash}131},
	type = {Article},
	abstract = {Dike intrusion is a fundamental process during upper oceanic crustal accretion at fast- to superfast-spreading ridges. Based on the distribution of magma along fast-spreading centers inferred from marine geophysical data, models predict systematic steep flow at magmatically robust segment centers and shallow magma flow toward distal segment ends. Anisotropy of magnetic susceptibility (AMS) fabrics from 48 fully-oriented block samples of dikes from upper oceanic crust exposed at Hess Deep Rift and Pito Deep Rift reveal a wide range of magma flow directions that are not consistent with such simple magma supply models. The AMS is interpreted to arise from distribution anisotropy of titanomagnetite crystals based on weak shape-preferred orientation of opaque oxide and plagioclase crystals generally parallel to AMS maximum eigenvectors. Most dike samples show normal AMS fabrics with maximum eigenvector directions ranging from subvertical to subhorizontal. The distributions of inferred magma flow lineations from maximum eigenvectors show no preferred flow pattern, even after structural correction. We use a Kolmogorov Smirnov test (KS-test) to show that the distribution of bootstrapped flow lineation rakes from Pito Deep are not statistically distinct from Hess Deep, and neither are distinguishable from Oman and Troodos Ophiolite AMS data. Magma flow directions in sheeted dikes from these two seafloor escarpments also do not correlate with available geochemistry in any systematic way as previously predicted. These results indicate distinct compositional sources feed melt that is injected into dikes at fast- to superfast-spreading ridges with no preference for subhorizontal or subvertical magma flow. Collectively, results imply ephemeral melt lenses at different along-axis locations within the continuous axial magma chamber and either direct injection or intermingling of melt from other deeper ridge-centered or off-axis sources. (C) 2014 Elsevier B.V. All rights reserved.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0012-821X},
	doi = {10.1016/j.epsl.2014.09.022},
	author = {Horst, A J and Varga, R J and Gee, J S and Karson, J A}
}
@article {ISI:000345292900023,
	title = {{Diversity and abundance of Bacteria and nirS-encoding denitrifiers associated with the Juan de Fuca Ridge hydrothermal system}},
	journal = {ANNALS OF MICROBIOLOGY},
	volume = {64},
	number = {4},
	year = {2014},
	month = {dec},
	pages = {1691{\textendash}1705},
	type = {Article},
	abstract = {Denitrification, which results in the loss of bioavailable nitrogen-an essential macronutrient for all living organisms-may potentially affect chemosynthetic primary production in hydrothermal vent ecosystems where sub-oxic conditions favorable to denitrification are common. Here we describe the diversity and abundance of denitrifying bacteria in the subsurface biosphere at Axial Volcano and the Endeavour Segment on the Juan de Fuca Ridge using a combination of quantitative polymerase chain reaction assays, and small subunit ribosomal RNA (SSU or 16S rRNA) pyrotag and nitrite reductase (nirS) clone library sequencing methods. Bacterial communities were diverse and dominated by members of the epsilon- and gamma-proteobacteria, including taxonomic groups containing known denitrifiers. Assemblages of denitrifiers that could be evaluated by nirS gene sequence comparisons showed low diversity. The single nirS sequence shared by the two locations, affiliated with a gamma-proteobacteria isolated from estuarine sediments (Pseudomonas sp. BA2), represented more than half of all sequences recovered when clustered at 97 {\%} identity. All other nirS sequences clustered into different taxonomic groups, indicating important differences in denitrifier community membership between the two sites. Total nirS gene abundance was at least two orders of magnitude lower than 16S rRNA abundance. Overall, our results demonstrate that the diversity and abundance of the nirS gene-containing bacterial community are rather low, as might be expected under the extreme conditions encountered in the subsurface biosphere of hydrothermal vent systems, and do not correlate clearly with any environmental variables investigated (i.e., pH, temperature, and H2S, NO3-, NH4+ concentrations).},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {1590-4261},
	doi = {10.1007/s13213-014-0813-3},
	author = {Bourbonnais, Annie and Juniper, S Kim and Butterfield, David A and Anderson, Rika E and Lehmann, Moritz F}
}
@article {Embley2014,
	title = {{Eruptive modes and hiatus of volcanism at West Mata seamount, NE Lau basin: 1996{\textendash}2012}},
	journal = {Geochemistry, Geophysics, Geosystems},
	volume = {15},
	number = {10},
	year = {2014},
	pages = {4093{\textendash}4115},
	abstract = {We present multiple lines of evidence for years to decade-long changes in the location and character of volcanic activity at West Mata seamount in the NE Lau basin over a 16 year period, and a hiatus in summit eruptions from early 2011 to at least September 2012. Boninite lava and pyroclasts were observed erupting from its summit in 2009, and hydroacoustic data from a succession of hydrophones moored nearby show near-continuous eruptive activity from January 2009 to early 2011. Successive differencing of seven multibeam bathymetric surveys of the volcano made in the 1996{\textendash}2012 period reveals a pattern of extended constructional volcanism on the summit and northwest flank punctuated by eruptions along the volcano{\textquoteright}s WSW rift zone (WSWRZ). Away from the summit, the volumetrically largest eruption during the observational period occurred between May 2010 and November 2011 at \~{}2920 m depth near the base of the WSWRZ. The (nearly) equally long ENE rift zone did not experience any volcanic activity during the 1996{\textendash}2012 period. The cessation of summit volcanism recorded on the moored hydrophone was accompanied or followed by the formation of a small summit crater and a landslide on the eastern flank. Water column sensors, analysis of gas samples in the overlying hydrothermal plume and dives with a remotely operated vehicle in September 2012 confirmed that the summit eruption had ceased. Based on the historical eruption rates calculated using the bathymetric differencing technique, the volcano could be as young as several thousand years.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1002/2014GC005387},
	author = {Embley, R W and Merle, S G and Baker, E T and Rubin, K H and Lupton, J E and Resing, J A and Dziak, R P and Lilley, M D and Chadwick, W W and Shank, T M and Greene, R and Walker, S L and Haxel, J H and Olson, E J and Baumberger, T}
}
@article {Brooke2014,
	title = {{First observations of the cold-water coral Lophelia pertusa in mid-Atlantic canyons of the USA}},
	journal = {Deep-Sea Research. Part II: Topical Studies in Oceanography},
	volume = {104},
	year = {2014},
	pages = {245{\textendash}251},
	abstract = {The structure-forming, cold-water coral Lophelia pertusa is widely distributed throughout the North Atlantic Ocean and also occurs in the South Atlantic, North Pacific and Indian oceans. This species has formed extensive reefs, chiefly in deep water, along the continental margins of Europe and the United States, particularly off the southeastern U.S. coastline and in the Gulf of Mexico. There were, however, no records of L. pertusa between the continental slope off Cape Lookout, North Carolina (NC) (\~{}34{\textdegree}N, 76{\textdegree}W), and the rocky Lydonia and Oceanographer canyons off Cape Cod, Massachusetts (MA) (\~{}40{\textdegree}N, 68{\textdegree}W). During a research cruise in September 2012, L. pertusa colonies were observed on steep walls in both Baltimore and Norfolk canyons. These colonies were all approximately 2 m or less in diameter, usually hemispherical in shape and consisted entirely of live polyps. The colonies were found between 381 m and 434 m with environmental observations of: temperature 6.4{\textendash}8.6 {\textdegree}C; salinity 35.0{\textendash}35.6; and dissolved oxygen 2.06{\textendash}4.41 ml L-1, all of which fall within the range of known L. pertusa distributions. All colonies were observed on vertical walls or underneath overhangs in areas of high current, which differs from observations further south, where L. pertusa colonizes rocky ledges and outcroppings, often forming large bioherms. We discuss observations from Baltimore and Norfolk canyons in the context of the known distribution of this species in the North Atlantic.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0645},
	doi = {10.1016/j.dsr2.2013.06.011},
	url = {http://www.sciencedirect.com/science/article/pii/S0967064513002415},
	author = {Brooke, S and Ross, S W}
}
@article {Thal2014,
	title = {{Geologic setting of PACManus hydrothermal area - High resolution mapping and in situ observations}},
	journal = {Marine Geology},
	volume = {355},
	year = {2014},
	pages = {98{\textendash}114},
	abstract = {This study presents a systematic analysis and interpretation of autonomous underwater vehicle-based microbathymetry combined with remotely operated vehicle (ROV) video recordings, rock analyses and temperature measurements within the PACManus hydrothermal area located on Pual Ridge in the Bismarck Sea of eastern Manus Basin. The data obtained during research cruise Magellan-06 and So-216 provides a framework for understanding the relationship between the volcanism, tectonism and hydrothermal activity. PACManus is a submarine felsic vocanically-hosted hydrothermal area that hosts multiple vent fields located within several hundred meters of one another but with different fluid chemistries, vent temperatures and morphologies. The total area of hydrothermal activity is estimated to be 20,279 m(2). The microbathymetry maps combined with the ROV video observations allow for precise high-resolution mapping estimates of the areal extents of hydrothermal activity. We find the distribution of hydrothermal fields in the PACManus area is primarily controlled by volcanic features that include lava domes, thick and massive blocky lava flows, breccias and feeder dykes. Spatial variation in the permeability of local volcanic facies appears to control the distribution of venting within a field. We define a three-stage chronological sequence for the volcanic evolution of the PACManus based on lava flow morphology, sediment cover and lava SiO2 concentration. In Stage-1, sparsely to moderately porphyritic dacite lavas (68-69.8 wt.{\%} SiO2) erupted to form domes or cryptodomes. In Stage-2, aphyric lava with slightly lower SiO2 concentrations (67.2-67.9 wt.{\%} SiO2) formed jumbled and pillowed lava flows. In the most recent phase Stage-3, massive blocky lavas with 69 to 72.5 wt{\%} SiO2 were erupted through multiple vents constructing a volcanic ridge identified as the PACManus neovolcanic zone. The transition between these stages may be gradual and related to progressive heating of a silicic magma following a recharge event of hot, mantle-derived melts.},
	keywords = {AUV ABE (Autonomous Benthic Explorer), ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.margeo.2014.05.011},
	author = {Thal, Janis and Tivey, Maurice and Yoerger, Dana and Joens, Niels and Bach, Wolfgang}
}
@article {ISI:000336392800014,
	title = {{Greenhouse gases generated from the anaerobic biodegradation of natural offshore asphalt seepages in southern California}},
	journal = {GEO-MARINE LETTERS},
	volume = {34},
	number = {2-3},
	year = {2014},
	month = {jun},
	pages = {281{\textendash}295},
	type = {Article; Proceedings Paper},
	abstract = {Significant offshore asphaltic deposits with active seepage occur in the Santa Barbara Channel offshore southern California. The composition and isotopic signatures of gases sampled from the oil and gas seeps reveal that the coexisting oil in the shallow subsurface is anaerobically biodegraded, generating CO2 with secondary CH4 production. Biomineralization can result in the consumption of as much as 60{\%} by weight of the original oil, with C-13 enrichment of CO2. Analyses of gas emitted from asphaltic accumulations or seeps on the seafloor indicate up to 11{\%} CO2 with C-13 enrichment reaching +24.8aEuro degrees. Methane concentrations range from less than 30{\%} up to 98{\%} with isotopic compositions of -34.9 to -66.1aEuro degrees. Higher molecular weight hydrocarbon gases are present in strongly varying concentrations reflecting both oil-associated gas and biodegradation; propane is preferentially biodegraded, resulting in an enriched C-13 isotopic composition as enriched as -19.5aEuro degrees. Assuming the 132 million barrels of asphaltic residues on the seafloor represent similar to 40{\%} of the original oil volume and mass, the estimated gas generated is 5.0x10(10) kg (similar to 76x10(9) m(3)) CH4 and/or 1.4x10(11) kg CO2 over the lifetime of seepage needed to produce the volume of these deposits. Geologic relationships and oil weathering inferences suggest the deposits are of early Holocene age or even younger. Assuming an age of similar to 1,000 years, annual fluxes are on the order of 5.0x10(7) kg (similar to 76x10(6) m(3)) and/or 1.4x10(8) kg for CH4 and CO2, respectively. The daily volumetric emission rate (2.1x10(5) m(3)) is comparable to current CH4 emission from Coal Oil Point seeps (1.5x10(5) m(3)/day), and may be a significant source of both CH4 and CO2 to the atmosphere provided that the gas can be transported through the water column.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0276-0460},
	doi = {10.1007/s00367-014-0359-1},
	author = {Lorenson, Thomas D and Wong, Florence L and Dartnell, Peter and Sliter, Ray W}
}
@article {ISI:000338120000010,
	title = {{High-resolution ecological niche modelling of the cold-water coral Lophelia pertusa in the Gulf of Mexico}},
	journal = {MARINE ECOLOGY PROGRESS SERIES},
	volume = {506},
	year = {2014},
	pages = {145{\textendash}U454},
	type = {Article},
	abstract = {The niche of many deep-sea species remains poorly resolved despite decades of seafloor exploration. Without better information on the distribution and habitat preference of key species, a complete understanding of the ecology of deep-sea communities will remain unattainable. It is increasingly apparent that cold-water corals are among the dominant foundation species in the deep sea, providing both structurally complex habitat and significant ecosystem services. In this study, the niche and distribution of the cold-water coral Lophelia pertusa in the Gulf of Mexico was evaluated using the maximum entropy (Maxent) approach. Ecological niche models were constructed for a broad region of the northern Gulf of Mexico using data gridded at a spatial resolution of 25 m, including bathymetry, substrate type, export productivity, and aragonite saturation state at depth. Fine-scale models were constructed at a resolution of 5 m using only remotely sensed bathymetric and surface reflectivity data. The broad-scale model performed well, with an area under the curve (AUC) of 0.981. All fine-scale models performed well when verified using training data (average AUC of 0.963) and when validated using independent occurrence data from a new geographic region (average AUC of 0.937). The distribution of L. pertusa in the Gulf of Mexico was found to be controlled primarily by depth, local topography, and availability of hard substrate. While these factors have long been associated with the success of cold-water corals, their relative importance has never been quantified in the Gulf of Mexico, making it historically difficult to precisely delineate L. pertusa{\textquoteright}s niche and predict its distribution in unexplored regions. Given these results, we suggest that future expeditions combine remotely sensed data with niche modelling techniques to increase the efficiency of deep-sea exploration.},
	keywords = {AUV Sentry (Autonomous Underwater Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0171-8630},
	doi = {10.3354/meps10816},
	author = {Georgian, Samuel E and Shedd, William and Cordes, Erik E}
}
@article {Tivey2014,
	title = {{High-resolution near-bottom vector magnetic anomalies over Raven Hydrothermal Field, Endeavour Segment, Juan de Fuca Ridge}},
	journal = {Journal of Geophysical Research: Solid Earth},
	volume = {119},
	number = {10},
	year = {2014},
	pages = {7389{\textendash}7403},
	abstract = {High-resolution, near-bottom vector magnetic data were collected by remotely operated vehicle Jason over the Raven hydrothermal vent field (47{\textdegree}57.3'N 129{\textdegree}5.75'W) located north of Main Endeavour vent field on the Endeavour segment of the Juan de Fuca Ridge. The survey was part of a comprehensive heat flow study of the Raven site using innovative thermal blanket technology to map the heat flux and crustal fluid pathways around a solitary hydrothermal vent field. Raven hydrothermal activity is presently located along the western axial valley wall, while additional inactive hydrothermal deposits are found to the NW on the upper rift valley wall. Magnetic inversion results show discrete areas of reduced magnetization associated with both active and inactive hydrothermal vent deposits that also show high conductive heat flow. Higher spatial variability in the heat flow patterns compared to the magnetization is consistent with the heat flow reflecting the currently active but ephemeral thermal environment of fluid flow, while crustal magnetization is representative of the static time-averaged effect of hydrothermal alteration. A general NW to SE trend in reduced magnetization across the Raven area correlates closely with the distribution of hydrothermal deposits and heat flux patterns and suggests that the fluid circulation system at depth is likely controlled by local crustal structure and magma chamber geometry. Magnetic gradient tensor components computed from vector magnetic data improve the resolution of the magnetic anomaly source and indicate that the hydrothermally altered zone directly beneath the Raven site is approximately 15 {\texttimes} 106 m3 in volume.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1002/2014JB011223},
	author = {Tivey, M A and Johnson, H P and Salmi, M S and Hutnak, M}
}
@article {Jamieson2014,
	title = {{Hydrothermal sulfide accumulation along the Endeavour Segment, Juan de Fuca Ridge}},
	journal = {Earth and Planetary Science Letters},
	volume = {395},
	year = {2014},
	month = {jan},
	pages = {136{\textendash}148},
	abstract = {Hydrothermal sulfide deposits that form on the seafloor are often located by the detection of hydrothermal plumes in the water column, followed by exploration with deep-towed cameras, side-scan sonar imaging, and finally by visual surveys using remotely-operated vehicle or occupied submersible. Hydrothermal plume detection, however, is ineffective for finding hydrothermally-inactive sulfide deposits, which may represent a significant amount of the total sulfide accumulation on the seafloor, even in hydrothermally active settings. Here, we present results from recent high-resolution, autonomous underwater vehicle-based mapping of the hydrothermally-active Endeavour Segment of the Juan de Fuca Ridge, in the Northeast Pacific Ocean. Analysis of the ridge bathymetry resulted in the location of 581 individual sulfide deposits along 24 km of ridge length. Hydrothermal deposits were distinguished from volcanic and tectonic features based on the characteristics of their surface morphology, such as shape and slope angles. Volume calculations for each deposit results in a total volume of 372,500 m3 of hydrothermal sulfide{\textendash}sulfate{\textendash}silica material, for an equivalent mass of \~{}1.2 Mt of hydrothermal material on the seafloor within the ridge{\textquoteright}s axial valley, assuming a density of 3.1 g/cm3. Much of this total volume is from previously undocumented inactive deposits outside the main active vent fields. Based on minimum ages of sulfide deposition, the deposits accumulated at a maximum rate of \~{}400 t/yr, with a depositional efficiency (proportion of hydrothermal material that accumulates on the seafloor to the total amount hydrothermally mobilized and transported to the seafloor) of \~{}5{\%}. The calculated sulfide tonnage represents a four-fold increase over previous sulfide estimates for the Endeavour Segment that were based largely on accumulations from within the active fields. These results suggest that recent global seafloor sulfide resource estimates, which were based mostly on the sizes and distribution of hydrothermally-active deposits, may be similarly underestimating the amount of sulfide along the global submarine neovolcanic zones.},
	keywords = {AUV ABE (Autonomous Benthic Explorer), HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0012-821X},
	doi = {10.1016/j.epsl.2014.03.035},
	url = {http://www.sciencedirect.com/science/article/pii/S0012821X14001873},
	author = {Jamieson, J W and Clague, D A and Hannington, M D}
}
@article {ISI:000348060800012,
	title = {{Imaging of CO2 bubble plumes above an erupting submarine volcano, NW Rota-1, Mariana Arc}},
	journal = {GEOCHEMISTRY GEOPHYSICS GEOSYSTEMS},
	volume = {15},
	number = {11},
	year = {2014},
	month = {nov},
	pages = {4325{\textendash}4342},
	type = {Article},
	abstract = {NW Rota-1 is a submarine volcano in the Mariana volcanic arc located similar to 100 km north of Guam. Underwater explosive eruptions driven by magmatic gases were first witnessed there in 2004 and continued until at least 2010. During a March 2010 expedition, visual observations documented continuous but variable eruptive activity at multiple vents at similar to 560 m depth. Some vents released CO2 bubbles passively and continuously, while others released CO2 during stronger but intermittent explosive bursts. Plumes of CO2 bubbles in the water column over the volcano were imaged by an EM122 (12 kHz) multibeam sonar system. Throughout the 2010 expedition numerous passes were made over the eruptive vents with the ship to document the temporal variability of the bubble plumes and relate them to the eruptive activity on the seafloor, as recorded by an in situ hydrophone and visual observations. Analysis of the EM122 midwater data set shows: (1) bubble plumes were present on every pass over the summit and they rose 200-400 m above the vents but dissolved before they reached the ocean surface, (2) bubble plume deflection direction and distance correlate well with ocean current direction and velocity determined from the ship{\textquoteright}s acoustic doppler current profiler, (3) bubble plume heights and volumes were variable over time and correlate with eruptive intensity as measured by the in situ hydrophone. This study shows that midwater multibeam sonar data can be used to characterize the level of eruptive activity and its temporal variability at a shallow submarine volcano with robust CO2 output.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1525-2027},
	doi = {10.1002/2014GC005543},
	author = {Chadwick Jr., William W and Merle, Susan G and Buck, Nathaniel J and Lavelle, J William and Resing, Joseph A and Ferrini, Vicki}
}
@article {Doughty2014,
	title = {{Insights into the population dynamics of the deep-sea coral genus Paramuricea in the Gulf of Mexico}},
	journal = {Biology and Geology of Deep-Sea Coral Ecosystems: Proceedings of the Fifth International Symposium on Deep Sea Corals},
	volume = {99},
	year = {2014},
	pages = {71{\textendash}82},
	abstract = {Species in the genus Paramuricea are among the most common corals in deep waters ({\textgreater}200~m) of the Gulf of Mexico. Paramuricea spp. increase habitat heterogeneity and provide substrate for numerous faunal associates, including ophiuroids that occur on the majority of coral colonies. In light of the Deepwater Horizon oil spill in the Gulf of Mexico, it has become apparent that there is a critical need for data on population dynamics within this genus. To date, at least one species of Paramuricea (P. biscaya) is known to be negatively impacted by the spill. Using remotely operated vehicles from 2009 to 2011, we documented the density and size frequency distributions of Paramuricea across 21 sites at depths of 250{\textendash}2500~m. Molecular barcoding (mtCOI+igr+MutS) was used to delineate species boundaries among the specimens collected. Results suggested that seven haplotypes are present in the Gulf, and appear to be partially segregated by depth [(type H: was used to delineate species boundaries among the specimens collected. Results suggested that seven haplotypes are present in the Gulf, and appear to be partially segregated by depth [(type H: Paramuricea spp. determined by ROV surveys were mapped onto high-resolution bathymetric data, which confirmed the corals{\textquoteright} preference for topographic highs composed of hard substrata. At any one site, densities ranged from 0.043{\textpm}0.01 (VK906, 380~m) to 1.18{\textpm}0.81~colonies/m2 (GC852, 1410~m). Mortality and recruitment rates were estimated from size-frequency data combined with estimated growth rates. Mortality rate decreased with size, from approximately 20{\%} and 70{\%} in new recruits of Paramuricea B3 and P. biscaya respectively, to less than 10{\%} in colonies over 40~cm in both species. Recruitment rates were estimated from less than one to over 30 individuals per year per site, but patterns in size-frequency histograms suggest that this process is highly variable at the different sites and at different times. These data illustrate that populations of the slow-growing Paramuricea species are sparsely distributed and exhibit low recruitment rates, making them highly susceptible to anthropogenic threats.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0645},
	doi = {10.1016/j.dsr2.2013.05.023},
	url = {http://www.sciencedirect.com/science/article/pii/S0967064513002142},
	author = {Doughty, C L and Quattrini, A M and Cordes, E E}
}
@article {ISI:000345702700014,
	title = {{Intracellular Oceanospirillales inhabit the gills of the hydrothermal vent snail Alviniconcha with chemosynthetic, gamma-Proteobacterial symbionts}},
	journal = {ENVIRONMENTAL MICROBIOLOGY REPORTS},
	volume = {6},
	number = {6},
	year = {2014},
	month = {dec},
	pages = {656{\textendash}664},
	type = {Article},
	abstract = {Associations between bacteria from the -Proteobacterial order Oceanospirillales and marine invertebrates are quite common. Members of the Oceanospirillales exhibit a diversity of interactions with their various hosts, ranging from the catabolism of complex compounds that benefit host growth to attacking and bursting host nuclei. Here, we describe the association between a novel Oceanospirillales phylotype and the hydrothermal vent snail Alviniconcha. Alviniconcha typically harbour chemoautotrophic - or epsilon-Proteobacterial symbionts inside their gill cells. Via fluorescence in situ hybridization and transmission electron microscopy, we observed an Oceanospirillales phylotype (named AOP for AlviniconchaOceanospirillales phylotype{\textquoteright}) in membrane-bound vacuoles that were separate from the known - or epsilon-Proteobacterial symbionts. Using quantitative polymerase chain reaction, we surveyed 181 Alviniconcha hosting -Proteobacterial symbionts and 102 hosting epsilon-Proteobacterial symbionts, and found that the population size of AOP was always minor relative to the canonical symbionts (median 0.53{\%} of the total quantified 16S rRNA genes). Additionally, we detected AOP more frequently in Alviniconcha hosting -Proteobacterial symbionts than in those hosting epsilon-Proteobacterial symbionts (96{\%} and 5{\%} of individuals respectively). The high incidence of AOP in -Proteobacteria hosting Alviniconcha implies that it could play a significant ecological role either as a host parasite or as an additional symbiont with unknown physiological capacities.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1758-2229},
	doi = {10.1111/1758-2229.12183},
	author = {Beinart, R A and Nyholm, S V and Dubilier, N and Girguis, P R}
}
@article {ISI:000345820100015,
	title = {{A large volume particulate and water multi-sampler with in situ preservation for microbial and biogeochemical studies}},
	journal = {DEEP-SEA RESEARCH PART I-OCEANOGRAPHIC RESEARCH PAPERS},
	volume = {94},
	year = {2014},
	month = {dec},
	pages = {195{\textendash}206},
	type = {Article},
	abstract = {A new tool was developed for large volume sampling to facilitate marine microbiology and biogeochemical studies. It was developed for remotely operated vehicle and hydrocast deployments, and allows for rapid collection of multiple sample types from the water column and dynamic, variable environments such as rising hydrothermal plumes. It was used successfully during a cruise to the hydrothermal vent systems of the Mid-Cayman Rise. The Suspended Particulate Rosette V2 large volume multi-sampling system allows for the collection of 14 sample sets per deployment. Each sample set can include filtered material, whole (unfiltered) water, and filtrate. Suspended particulate can be collected on filters up to 142 mm in diameter and pore sizes down to 0.2 mu m. Filtration is typically at flowrates of 2 L min(-1) For particulate material, filtered volume is constrained only by sampling time and filter capacity, with all sample volumes recorded by digital flowmeter. The suspended particulate filter holders can be filled with preservative and sealed immediately after sample collection. Up to 2 L of whole water, filtrate, or a combination of the two, can be collected as part of each sample set. The system is constructed of plastics with titanium fasteners and nickel alloy spring loaded seals. There are no ferrous alloys in the sampling system. Individual sample lines are prefilled with filtered, deionized water prior to deployment and remain sealed unless a sample is actively being collected. This system is intended to facilitate studies concerning the relationship between marine microbiology and ocean biogeochemistry. (C) 2014 Elsevier Ltd. All rights reserved.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0637},
	doi = {10.1016/j.dsr.2014.08.008},
	author = {Breier, J A and Sheik, C S and Gomez-Ibanez, D and Sayre-McCord, R T and Sanger, R and Rauch, C and Coleman, M and Bennett, S A and Cron, B R and Li, M and German, C R and Toner, B M and Dick, G J}
}
@article {Koschinsky2014,
	title = {{Metal concentrations in the tissues of the hydrothermal vent mussel Bathymodiolus: Reflection of different metal sources}},
	journal = {Marine Environmental Research},
	volume = {95},
	year = {2014},
	pages = {62{\textendash}73},
	abstract = {Hydrothermal vent mussels of the genus Bathymodiolus are ideally positioned for the use of recording hydrothermal fluxes at the hydrothermal vent sites they inhabit. Barium, Ca, Cd, Co, Cr, Cu, Fe, K, Mg, Mn, Mo, Pb, Sr, and U concentrations in tissue sections of Bathymodiolus mussels from several hydrothermal fields between 15{\textdegree}N and 9{\textdegree}S at the Mid-Atlantic Ridge were determined and compared to the surrounding fluids and solid substrates in the habitats. Elements generally enriched in hydrothermal fluids, such as Fe, Cu, Zn, Pb and Cd, were significantly enriched in the gills and digestive glands of the hydrothermal mussels. The rather small variability of Zn (and Mn) and positive correlation with K and earth alkaline metals may indicate a biological regulation of accumulation. Enrichments of Mo and U in many tissue samples indicate that particulate matter such as hydrothermal mineral particles from the plumes can play a more important role as a metal source than dissolved metals. Highest enrichments of Cu in mussels from the Golden Valley site indicate a relation to the >=400 {\textdegree}C hot heavy-metal rich fluids emanating in the vicinity. In contrast, mussels from the low-temperature Lilliput field are affected by the Fe oxyhydroxide sediment of their habitat. In a comparison of two different sites within the Logatchev field metal distributions in the tissues reflected small-scale local variations in the metal content of the fluids and the particulate material.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0141-1136},
	doi = {10.1016/j.marenvres.2013.12.012},
	url = {http://www.sciencedirect.com/science/article/pii/S0141113613002249},
	author = {Koschinsky, A and Kausch, M and Borowski, C}
}
@article {ISI:000331788900001,
	title = {{Microsporidia-nematode associations in methane seeps reveal basal fungal parasitism in the deep sea}},
	journal = {FRONTIERS IN MICROBIOLOGY},
	volume = {5},
	year = {2014},
	month = {feb},
	type = {Article},
	abstract = {The deep sea is Earth{\textquoteright}s largest habitat but little is known about the nature of deep-sea parasitism. In contrast to a few characterized cases of bacterial and protistan parasites, the existence and biological significance of deep-sea parasitic fungi is yet to be understood. Here we report the discovery of a fungus-related parasitic microsporidium, Nematocenator marisprofundi n. gen. n. sp. that infects benthic nematodes at methane seeps on the Pacific Ocean floor. This infection is species-specific and has been temporally and spatially stable over 2 years of sampling, indicating an ecologically consistent host-parasite interaction. A high distribution of spores in the reproductive tracts of infected males and females and their absence from host nematodes{\textquoteright} intestines suggests a sexual transmission strategy in contrast to the fecal-oral transmission of most microsporidia. N. mansprofundi targets the host{\textquoteright}s body wall muscles causing cell lysis, and in severe infection even muscle filament degradation. Phylogenetic analyses placed N. marisprofundi in a novel and basal clade not closely related to any described microsporidia clade, suggesting either that microsporidia-nematode parasitism occurred early in microsporidia evolution or that host specialization occurred late in an ancient deep-sea microsporidian lineage. Our findings reveal that methane seeps support complex ecosystems involving interkingdom interactions between bacteria, nematodes, and parasitic fungi and that microsporidia parasitism exists also in the deep-sea biosphere.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {1664-302X},
	doi = {10.3389/fmicb.2014.00043},
	author = {Sapir, Amir and Dillman, Adler R and Connon, Stephanie A and Grupe, Benjamin M and Ingels, Jeroen and Mundo-Ocampo, Manuel and Levin, Lisa A and Baldwin, James G and Orphan, Victoria J and Sternberg, Paul W}
}
@article {ISI:000343852900015,
	title = {{Molluscan species diversity at North Pacific hydrothermal vents: What we know and what it may mean}},
	journal = {AMERICAN MALACOLOGICAL BULLETIN},
	volume = {32},
	number = {2},
	year = {2014},
	month = {sep},
	pages = {267{\textendash}277},
	type = {Article},
	abstract = {Hydrothermal vents in the deep sea are harsh, temporally unpredictable habitats with what appears to be a distinct fauna. Decades of subsea research with crewed and remote vehicles have generated a list of known species; is the species list complete? Evidence derived from mollusks sampled at hydrothermal vents on the East Pacific Rise (EPR), and Gorda and Juan de Fuca ridges suggests that the answer is yes. A 2006 compilation of hydrothermal vent species based on decades of research is updated and compared to specimens from these three active ridges in collections of the Field Museum of Natural History (FMNH) that resulted from limited collecting activity. Only three dives at each of the two Gorda Ridge vents collected all named species; 90{\%} of the 20 species known from Juan de Fuca Ridge were collected in nine cruises. At the EPR, only 81{\%} of the 43 known species were collected, but differences among the ridges were not significant. The limited FMNH collections increased the known ranges of six species from Juan de Fuca to Gorda Ridge and of nine species on the EPR. In addition, the EPR appears to host more rare species, potentially due to the frequent temporal changes at these vents. Mollusks currently known from each ridge, with their expanded ranges, are listed; the implications of these results for recent discoveries of slow-spreading vent fields are discussed.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0740-2783},
	author = {Voight, Janet R}
}
@article {Gartman2014,
	title = {{Nanoparticulate pyrite and other nanoparticles are a widespread component of hydrothermal vent black smoker emissions}},
	journal = {Chemical Geology},
	volume = {366},
	year = {2014},
	pages = {32{\textendash}41},
	abstract = {The presence of nanoparticulate pyrite is reported in hydrothermal emissions from Rainbow, TAG and Snakepit on the Mid-Atlantic Ridge (MAR). When coupled with previously collected data from East Pacific Rise 9{\textdegree}N (EPR) and Lau Basin, these data demonstrate that pyrite nanoparticles are a widespread component of black smoker emissions from hydrothermal vents and are found in significant concentrations at a fast spreading mid-ocean Ridge (EPR), a back-arc basin (Lau Basin), and a slow spreading mid-ocean Ridge (MAR). The maximum percentage of filtered iron emitted as nanoparticulate pyrite was found to be as high as 25{\%}, 10{\%}, and 5{\%}, respectively. As a widespread component of hydrothermal vent emissions, these nanoparticles may be an important source of iron to the world{\textquoteright}s oceans. Metals such as Cu and Zn are detected in pyrite-containing aggregates at all sites, and chalcopyrite was a component of nanoparticle aggregates at MAR. Iron containing silicate nanoparticles are also identified, and indicate that nanoparticles other than sulfides should also be considered when determining transport implications of hydrothermal vent emissions. The varied morphologies and the presence of different minerals within these nanoparticles provide insight into their formation and stability.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0009-2541; 1878-5999},
	doi = {10.1016/j.chemgeo.2013.12.013},
	author = {Gartman, A and Findlay, A J and Luther, G W}
}
@article {ISI:000328605200015,
	title = {{Nitrate-based niche differentiation by distinct sulfate-reducing bacteria involved in the anaerobic oxidation of methane}},
	journal = {ISME JOURNAL},
	volume = {8},
	number = {1},
	year = {2014},
	month = {jan},
	pages = {150{\textendash}163},
	type = {Article},
	abstract = {Diverse associations between methanotrophic archaea (ANME) and sulfate-reducing bacterial groups (SRB) often co-occur in marine methane seeps; however, the ecophysiology of these different symbiotic associations has not been examined. Here, we applied a combination of molecular, geochemical and Fluorescence in situ hybridization (FISH) coupled to nanoscale secondary ion mass spectrometry (FISH-NanoSIMS) analyses of in situ seep sediments and methane-amended sediment incubations from diverse locations (Eel River Basin, Hydrate Ridge and Costa Rican Margin seeps) to investigate the distribution and physiology of a newly identified subgroup of the Desulfobulbaceae (seepDBB) found in consortia with ANME-2c archaea, and compared these with the more commonly observed associations between the same ANME partner and the Desulfobacteraceae (DSS). FISH analyses revealed aggregates of seepDBB cells in association with ANME-2 from both environmental samples and laboratory incubations that are distinct in their structure relative to co-occurring ANME/DSS consortia. ANME/seepDBB aggregates were most abundant in shallow sediment depths below sulfide-oxidizing microbial mats. Depth profiles of ANME/seepDBB aggregate abundance revealed a positive correlation with elevated porewater nitrate relative to ANME/DSS aggregates in all seep sites examined. This relationship with nitrate was supported by sediment microcosm experiments, in which the abundance of ANME/seepDBB was greater in nitrate-amended incubations relative to the unamended control. FISH-NanoSIMS additionally revealed significantly higher N-15-nitrate incorporation levels in individual aggregates of ANME/seepDBB relative to ANME/DSS aggregates from the same incubation. These combined results suggest that nitrate is a geochemical effector of ANME/seepDBB aggregate distribution, and provides a unique niche for these consortia through their utilization of a greater range of nitrogen substrates than the ANME/DSS.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {1751-7362},
	doi = {10.1038/ismej.2013.147},
	author = {Green-Saxena, A and Dekas, A E and Dalleska, N F and Orphan, V J}
}
@article {ISI:000334288600024,
	title = {{The origin of methanethiol in midocean ridge hydrothermal fluids}},
	journal = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
	volume = {111},
	number = {15},
	year = {2014},
	month = {apr},
	pages = {5474{\textendash}5479},
	type = {Article},
	abstract = {Simple alkyl thiols such as methanethiol (CH3SH) are widely speculated to form in seafloor hot spring fluids. Putative CH3SH synthesis by abiotic (nonbiological) reduction of inorganic carbon (CO2 or CO) has been invoked as an initiation reaction for the emergence of protometabolism and microbial life in primordial hydrothermal settings. Thiols are also presumptive ligands for hydrothermal trace metals and potential fuels for associated microbial communities. In an effort to constrain sources and sinks of CH3SH in seafloor hydrothermal systems, we determined for the first time its abundance in diverse hydrothermal fluids emanating from ultramafic, mafic, and sediment-covered midocean ridge settings. Our data demonstrate that the distribution of CH3SH is inconsistent with meta-stable equilibrium with inorganic carbon, indicating that production by abiotic carbon reduction is more limited than previously proposed. CH3SH concentrations are uniformly low (similar to 10(-8) M) in high-temperature fluids ({\textgreater}200 degrees C) from all unsedimented systems and, in many cases, suggestive of meta-stable equilibrium with CH4 instead. Associated low-temperature fluids ({\textless}200 degrees C) formed by admixing of seawater, however, are invariably enriched in CH3SH (up to similar to 10-6 M) along with NH4+ and low-molecular-weight hydrocarbons relative to high-temperature source fluids, resembling our observations from a sediment-hosted system. This strongly implicates thermogenic interactions between upwelling fluids and microbial biomass or associated dissolved organic matter during subsurface mixing in crustal aquifers. Widespread thermal degradation of subsurface organic matter may be an important source of organic production in unsedimented hydrothermal systems and may influence microbial metabolic strategies in cooler near-sea-floor and plume habitats.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0027-8424},
	doi = {10.1073/pnas.1400643111},
	author = {Reeves, Eoghan P and McDermott, Jill M and Seewald, Jeffrey S}
}
@article {Quattrini2014,
	title = {{A phylogenetic approach to octocoral community structure in the deep Gulf of Mexico}},
	journal = {Biology and Geology of Deep-Sea Coral Ecosystems: Proceedings of the Fifth International Symposium on Deep Sea Corals},
	volume = {99},
	year = {2014},
	pages = {92{\textendash}102},
	abstract = {{Deep-sea communities are becoming increasingly vulnerable to anthropogenic disturbances, as fishing, hydrocarbon exploration and extraction, and mining activities extend into deeper water. Negative impacts from such activities were recently documented in the Gulf of Mexico (GoM), where the Deepwater Horizon oil spill caused substantial damage to a deep-water octocoral community. Although a faunal checklist and numerous museum records are currently available for the entire GoM, local-scale diversity and assemblage structure of octocoral communities remains unknown, particularly in deep water. On a series of recent cruises (2008{\textendash}2011) using remotely operated vehicles, 435 octocorals were collected from 33 deep-water sites (250{\textendash}2500~m) in the northern GoM. To elucidate species boundaries, the extended mitochondrial barcode (COI+igr1+msh) was successfully amplified and sequenced for 422 of these specimens, yielding a total of 64 haplotypes representing at least 52 species. Further, at least 29{\%} of the species collected were either previously not known to occur in the GoM (12 species) or represent new species (at least three species). Overall, species richness at each site was fairly low (1{\textendash}12 spp.). The greatest species richness occurred at the shallowest () was successfully amplified and sequenced for 422 of these specimens, yielding a total of 64 haplotypes representing at least 52 species. Further, at least 29{\%} of the species collected were either previously not known to occur in the GoM (12 species) or represent new species (at least three species). Overall, species richness at each site was fairly low (1{\textendash}12 spp.). The greatest species richness occurred at the shallowest (n=8 spp.) and the deepest (2100{\textendash}2500~m: DC673},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0645},
	doi = {10.1016/j.dsr2.2013.05.027},
	url = {http://www.sciencedirect.com/science/article/pii/S096706451300218X},
	author = {Quattrini, A M and Etnoyer, P J and Doughty, C and English, Lisa and Falco, R and Remon, N and Rittinghouse, M and Cordes, E E}
}
@article {Nooner2014,
	title = {{Post Eruption inflation of the East Pacific Rise at 9 degrees 50 {\textquoteright} N}},
	journal = {Geochemistry, Geophysics, Geosystems},
	volume = {15},
	number = {6},
	year = {2014},
	pages = {2676{\textendash}2688},
	abstract = {In June 2008, we installed a geodetic network at 9 degrees 50{\textquoteright} N on the East Pacific Rise to track the long-term movement of magma following the 2005/6 eruption. This network consists of 10 concrete benchmarks stretching from the ridge to 9 km off-axis. During three campaign-style surveys, measurements of vertical seafloor motions were made at each of these benchmarks by precisely recording ambient seawater pressure as a proxy for seafloor depth with a mobile pressure recorder (MPR). The MPR was deployed using the manned submersible Alvin in 2008 and 2009 and the remotely operated vehicle Jason in 2011. The MPR observations are supplemented with data from a multiyear deployment of continuously recording bottom pressure recorders (BPRs) extending along this segment of the ridge that can record rapid changes in seafloor depth from seafloor eruptions and/or dike intrusions. These measurements show no diking events and up to 12 cm of volcanic inflation that occurred from December 2009 to October 2011 in the area of the 2005/6 eruption. These observations are fit with an inflating point source at a depth of 2.7 km and volume change of 2.3 x 10(6) m(3)/yr located on the ridge axis at approximately 9 degrees 51.166{\textquoteright} N, 407 m from our northernmost benchmark, suggesting that the magma chamber underlying this segment of the ridge is being recharged from a deeper source at a rate that is about half the long-term inflation rate observed at Axial Seamount on the Juan de Fuca Ridge. These data represent the second location that active volcanic uplift has been measured on a mid-ocean ridge segment, and the first on a nonhotspot influenced segment.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1002/2014GC005389},
	author = {Nooner, S L and Webb, S C and Buck, W R and Cormier, M H}
}
@article {Salmi2014,
	title = {{Quantitative estimate of heat flow from a mid-ocean ridge axial valley, Raven field, Juan de Fuca Ridge: Observations and inferences}},
	journal = {Journal of Geophysical Research: Solid Earth},
	volume = {119},
	number = {9},
	year = {2014},
	pages = {6841{\textendash}6854},
	abstract = {A systematic heat flow survey using thermal blankets within the Endeavour segment of the Juan de Fuca Ridge axial valley provides quantitative estimates of the magnitude and distribution of conductive heat flow at a mid-ocean ridge, with the goal of testing current models of hydrothermal circulation present within newly formed oceanic crust. Thermal blankets were deployed covering an area of 700 by 450 m in the Raven Hydrothermal vent field area located 400 m north of the Main Endeavour hydrothermal field. A total of 176 successful blanket deployment sites measured heat flow values that ranged from 0 to 31 W m-2. Approximately 53{\%} of the sites recorded values lower than 100 mW m-2, suggesting large areas of seawater recharge and advective extraction of lithospheric heat. High heat flow values were concentrated around relatively small {\textquotedblleft}hot spots.{\textquotedblright} Integration of heat flow values over the Raven survey area gives an estimate of conductive heat output of 0.3 MW, an average of 0.95 W m-2, over the survey area. Fluid circulation cell dimensions and scaling equations allow calculation of a Rayleigh number of approximately 700 in Layer 2A. The close proximity of high and low heat flow areas, coupled with previous estimates of surficial seafloor permeability, argues for the presence of small-scale hydrothermal fluid circulation cells within the high-porosity uppermost crustal layer of the axial seafloor.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1002/2014JB011086},
	author = {Salmi, M S and Johnson, H P and Tivey, M A and Hutnak, M}
}
@article {ISI:000341616500008,
	title = {{Spatial patterns of Aquificales in deep-sea vents along the Eastern Lau Spreading Center (SW Pacific)}},
	journal = {SYSTEMATIC AND APPLIED MICROBIOLOGY},
	volume = {37},
	number = {6},
	year = {2014},
	month = {sep},
	pages = {442{\textendash}448},
	type = {Article},
	abstract = {The microbial diversity associated with actively venting deep-sea hydrothermal deposits is tightly connected to the geochemistry of the hydrothermal fluids. Although the dominant members of these deposits drive the structure of the microbial communities, it is less well understood whether the lower abundance groups are as closely connected to the geochemical milieu, or driven perhaps by biotic factors such as microbial community interactions. We used the natural geochemical gradients that exist in the back-arc basin, Eastern Lau Spreading Center and Valu-Fa Ridge (ELSC/VFR) in the Southwestern Pacific, to explore whether the chemolithotrophic Aquificales are influenced by geographical location, host-rock of the vent field or deposit type. Using a combination of cloning, DNA fingerprinting (DGGE) and enrichment culturing approaches, all genera of this order previously described at marine vents were detected, i.e., Desulfurobacterium, Thermovibrio,Aquilex, Hydrogenivirga, Persephonella and Hydrogenothermus. The comparison between clone libraries and DGGE showed similar patterns of distribution of different Aquificales whereas results differed for the enrichment cultures that were retrieved. However, the use of cultivation-based and -independent methods did provide complementary phylogenetic diversity overview of the Aquificales in these systems. Together, this survey revealed that the ELSC/VFR contains some of the largest diversity of Aquificales ever reported at a deep-sea vent area, that the diversity patterns are tied to the geography and geochemistry of the system, and that this geochemical diverse back-arc basin may harbor new members of the Aquificales. (C) 2014 Elsevier GmbH. All rights reserved.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0723-2020},
	doi = {10.1016/j.syapm.2014.04.002},
	author = {Ferrera, Isabel and Banta, Amy B and Reysenbach, Anna-Louise}
}
@article {ISI:000332225300011,
	title = {{Spatial patterns of tissue stable isotope contents give insight into the nutritional sources for seep communities on the Gulf of Mexico lower slope}},
	journal = {MARINE ECOLOGY PROGRESS SERIES},
	volume = {498},
	year = {2014},
	pages = {133{\textendash}U481},
	type = {Article},
	abstract = {In this study, we present the first thorough trophic characterization of cold seep macrofaunal communities on the Gulf of Mexico lower continental slope ({\textgreater} 1000 m depth). We analyzed tissue delta C-13, delta N-15, and delta S-34 of vestimentiferan tubeworms, bathymodiolin mussels, vesicomyid clams, and their associated macrofaunal communities from discrete collections made across the entire lower slope. Over half of macrofauna associated with mussels and about half associated with vestimentiferans had delta C-13 values below -45{\%}. We also observed high spatial variability in the delta C-13 values of entire local communities, and the delta C-13 of associated fauna were significantly correlated with the delta C-13 compositions of the symbiotic species from the same location. These data indicate widespread incorporation of methane-derived carbon in mussel and vestimentiferan communities. This finding was particularly surprising in communities associated with older vestimentiferans, given the low rates of seepage observed in similar communities on the upper slope. On average, delta N-13 values in mussels and their associates were significantly more depleted and more variable than vestimentiferans, clams, and their associates, and there was a significant linear relationship between tissue delta N-15 values of mussels and their associated communities. The tissue delta S-34 values in macrofauna associated with vestimentiferans were more variable and significantly more depleted than mussel associates (delta S-34 = -16.8 to + 19.1{\%} for vestimentiferan associates and delta S-34 = -3.1 to + 20.8{\%} for mussel associates), consistent with higher isotopic fractionation during sulfate reduction in vestimentiferan habitats and a potentially higher nutritional contribution of sulfide-derived organic sulfur in vestimentiferan communities.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0171-8630},
	doi = {10.3354/meps10598},
	author = {Becker, Erin L and Cordes, Erik E and Macko, Stephen A and Lee, Raymond W and Fisher, Charles R}
}
@article {ISI:000333189700012,
	title = {{Strain-level genomic variation in natural populations of Lebetimonas from an erupting deep-sea volcano}},
	journal = {ISME JOURNAL},
	volume = {8},
	number = {4},
	year = {2014},
	month = {apr},
	pages = {867{\textendash}880},
	type = {Article},
	abstract = {Chemolithoautotrophic Epsilonproteobacteria are ubiquitous in sulfidic, oxygen-poor habitats, including hydrothermal vents, marine oxygen minimum zones, marine sediments and sulfidic caves and have a significant role in cycling carbon, hydrogen, nitrogen and sulfur in these environments. The isolation of diverse strains of Epsilonproteobacteria and the sequencing of their genomes have revealed that this group has the metabolic potential to occupy a wide range of niches, particularly at dynamic deep-sea hydrothermal vents. We expand on this body of work by examining the population genomics of six strains of Lebetimonas, a vent-endemic, thermophilic, hydrogen-oxidizing Epsilonproteobacterium, from a single seamount in the Mariana Arc. Using Lebetimonas as a model for anaerobic, moderately thermophilic organisms in the warm, anoxic subseafloor environment, we show that genomic content is highly conserved and that recombination is limited between closely related strains. The Lebetimonas genomes are shaped by mobile genetic elements and gene loss as well as the acquisition of novel functional genes by horizontal gene transfer, which provide the potential for adaptation and microbial speciation in the deep sea. In addition, these Lebetimonas genomes contain two operons of nitrogenase genes with different evolutionary origins. Lebetimonas expressed nifH during growth with nitrogen gas as the sole nitrogen source, thus providing the first evidence of nitrogen fixation in any Epsilonproteobacteria from deep-sea hydrothermal vents. In this study, we provide a comparative overview of the genomic potential within the Nautiliaceae as well as among more distantly related hydrothermal vent Epsilonproteobacteria to broaden our understanding of microbial adaptation and diversity in the deep sea.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1751-7362},
	doi = {10.1038/ismej.2013.206},
	author = {Meyer, Julie L and Huber, Julie A}
}
@article {Thresher2014,
	title = {{Strong Depth-Related Zonation of Megabenthos on a Rocky Continental Margin ( similar to 700-4000 m) off Southern Tasmania, Australia: e85872}},
	journal = {PLoS ONE},
	volume = {9},
	number = {1},
	year = {2014},
	publisher = {BioMed Central Ltd.},
	address = {United Kingdom},
	abstract = {Assemblages of megabenthos are structured in seven depth-related zones between {\~{}}700 and 4000 m on the rocky and topographically complex continental margin south of Tasmania, southeastern Australia. These patterns emerge from analysis of imagery and specimen collections taken from a suite of surveys using photographic and in situ sampling by epibenthic sleds, towed video cameras, an autonomous underwater vehicle and a remotely operated vehicle (ROV). Seamount peaks in shallow zones had relatively low biomass and low diversity assemblages, which may be in part natural and in part due to effects of bottom trawl fishing. Species richness was highest at intermediate depths (1000-1300 m) as a result of an extensive coral reef community based on the bioherm-forming scleractinian Solenosmilia variabilis. However, megabenthos abundance peaked in a deeper, low diversity assemblage at 2000-2500 m. The S. variabilis reef and the deep biomass zone were separated by an extensive dead, sub-fossil S. variabilis reef and a relatively low biomass stratum on volcanic rock roughly coincident with the oxygen minimum layer. Below 2400 m, megabenthos was increasingly sparse, though punctuated by occasional small pockets of relatively high diversity and biomass. Nonetheless, megabenthic organisms were observed in the vast majority of photographs on all seabed habitats and to the maximum depths observed - a sandy plain below 3950 m. Taxonomic studies in progress suggest that the observed depth zonation is based in part on changing species mixes with depth, but also an underlying commonality to much of the seamount and rocky substrate biota across all depths. Although the mechanisms supporting the extraordinarily high biomass in 2000-2500 m depths remains obscure, plausible explanations include equatorwards lateral transport of polar production and/or a response to depth-stratified oxygen availability.},
	keywords = {AUV ABE (Autonomous Benthic Explorer), ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1371/journal.pone.0085872},
	url = {http://norton.whoi.edu/sfx{\_}local?url{\_}ver=Z39.88-2004{\&}rft{\_}val{\_}fmt=info:ofi/fmt:kev:mtx:journal{\&}genre=article{\&}sid=ProQ:ProQ{\%}253Apqdibs{\&}atitle=Strong+Depth-Related+Zonation+of+Megabenthos+on+a+Rocky+Continental+Margin+{\%}2528+similar+to},
	author = {Thresher, R and Althaus, F and Adkins, J and Gowlett-Holmes, K and Alderslade, Phil and Dowdney, Jo and Cho, W and Gagnon, A and Staples, D and McEnnulty, F}
}
@article {ISI:000339334700007,
	title = {{Symbiont-driven sulfur crystal formation in a thiotrophic symbiosis from deep-sea hydrocarbon seeps}},
	journal = {ENVIRONMENTAL MICROBIOLOGY REPORTS},
	volume = {6},
	number = {4, SI},
	year = {2014},
	month = {aug},
	pages = {364{\textendash}372},
	type = {Article},
	abstract = {The siboglinid tubeworm Sclerolinum contortum symbiosis inhabits sulfidic sediments at deep-sea hydrocarbon seeps in the Gulf of Mexico. A single symbiont phylotype in the symbiont-housing organ is inferred from phylogenetic analyses of the 16S ribosomal ribonucleic acid (16S rRNA) gene and fluorescent in situ hybridization. The phylotype we studied here, and a previous study from an arctic hydrocarbon seep population, reveal identical 16S rRNA symbiont gene sequences. While sulfide is apparently the energy source for the symbionts (and ultimately the gutless host), both partners also have to cope with its toxicity. This study demonstrates abundant large sulfur crystals restricted to the trophosome area. Based on Raman microspectroscopy and energy dispersive X-ray analysis, these crystals have the same S8 sulfur configuration as the recently described small sulfur vesicles formed in the symbionts. The crystals reside adjacent to the symbionts in the trophosome. This suggests that their formation is either extra-or intracellular in symbionts. We propose that formation of these crystals provides both energy-storage compounds for the symbionts and serves the symbiosis by removing excess toxic sulfide from host tissues. This symbiont-mediated sulfide detoxification may have been crucial for the establishment of thiotrophic symbiosis and continues to remain an important function of the symbionts.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1758-2229},
	doi = {10.1111/1758-2229.12149},
	author = {Eichinger, Irmgard and Schmitz-Esser, Stephan and Schmid, Markus and Fisher, Charles R and Bright, Monika}
}
@article {Feng2014,
	title = {{Time integrated variation of sources of fluids and seepage dynamics archived in authigenic carbonates from Gulf of Mexico Gas Hydrate Seafloor Observatory}},
	journal = {Chemical Geology},
	volume = {385},
	year = {2014},
	pages = {129{\textendash}139},
	abstract = {Authigenic carbonate rocks recovered from the Gulf of Mexico Gas Hydrate Seafloor Observatory in Mississippi Canyon block 118 (MC118) at approximately 900 m water depth were studied using mineralogical, bulk geochemical, and lipid biomarker analyses. Carbonate rocks occurred as fractured blocks and nodular masses incorporated in carbonate breccias. The carbonates were comprised mainly of high-Mg-calcite and aragonite. The stable carbon isotope composition ($δ$13C) of authigenic carbonate varied from - 29.8{\textperthousand} to - 18.1{\textperthousand} vs. V-PDB, suggesting a complex mixture of various carbon sources, including dissolved marine inorganic carbon (DIC), oil, as well as methane. Oxygen isotopes ($δ$18O) varied from + 3.4{\textperthousand} to + 5.8{\textperthousand}. The observed 18O-enrichment in relation to calculated equilibrium values in the carbonates probably reflects decomposition of gas hydrates. The most abundant lipid biomarkers in the carbonates were isoprenoidal glycerol dibiphytanyl glycerol tetraethers (GDGTs), predominated by GDGT-2 and GDGT-3, which are typically indicators of anaerobic methane oxidizing archaea (ANMEs). Mono- and bicyclic biphytanes (derived after ether cleavage of GDGT-2 and GDGT-3) showed strong 13C-depletion, which is characteristic for ANMEs. Interestingly, large differences between the $δ$13C values of the archaeal diether archaeol and acyclic biphytane on the one hand and monocyclic biphytane on the other hand suggest the presence of archaea other than ANMEs. Archaeol and GDGT-0 (containing two acyclic biphytane moieties) are commonly assigned to various methanogenic archaea. Where methane seepage activity is intermediate or low within acoustic wipeout zones at the MC118 gas hydrate site nowadays, microbial communities must have coped with changing conditions as well as longer-term fluctuations in oil and gas seepage or the temporary cessation of hydrocarbon flux in the past. The change from methane seepage to oil seepage or vice versa in addition to flux variability apparently favors the establishment of complex prokaryotic communities dominated by archaea. In addition to anaerobic oxidation of methane, local production of methane is apparently prominent at the study site based on the occurrence of biomarkers of methanogens in the authigenic carbonate. This finding adds to the ongoing multidisciplinary effort to better constrain the environment at the MC118 observatory site and to determine the locally dominant biogeochemical processes.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.chemgeo.2014.07.020},
	author = {Feng, D and Birgel, D and Peckmann, J and Roberts, H H and Joye, S B and Sassen, R and Liu, X-L and Hinrichs, K -U and Chen, D}
}
@article {Baumberger2014,
	title = {{Understanding a submarine eruption through time series hydrothermal plume sampling of dissolved and particulate constituents: West Mata, 2008-2012}},
	journal = {Geochemistry, Geophysics, Geosystems},
	volume = {15},
	number = {12},
	year = {2014},
	pages = {4631{\textendash}4650},
	abstract = {Four cruises between 2008 and 2012 monitored the continuing eruption of West Mata volcano in the NE Lau Basin as it produced plumes of chemically altered water above its summit. Although large enrichments in He-3, CO2, Fe, and Mn were observed in the plumes, the most notable enrichment was that of H-2, which reached concentrations as high as 14,843 nM. Strongly enriched H-2 concentrations in the water column result from reactions between seawater or magmatic water and extremely hot rocks. In 2008, the observation of elevated H-2 concentrations in the water column above West Mata pointed to vigorous ongoing eruptions at the volcano{\textquoteright}s summit. The eruption was confirmed by visual observations made by the ROV Jason 2 in 2009 and demonstrated that H-2 measurements are a vital instrument to detect ongoing volcanic eruptions at the seafloor. Elevated H-2 in 2010 showed that the eruption was ongoing, although at a reduced level given a maximum H-2 concentration of 4410 nM. In 2012, H-2 levels in the water column declined significantly, to a maximum of only 7 nM, consistent with visual observations from the Quest-4000 ROV that found no evidence of an ongoing volcanic eruption. Methane behaved independently of other measured gases and its concentrations in the hydrothermal plume were very low. We attribute its minimal enrichments to a mixture of mantle carbon reduced to CH4 and biological CH4 from diffuse flow sites. This study demonstrates that ongoing submarine volcanic eruptions are characterized by high dissolved H-2 concentrations present in the overlying water column.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1002/2014GC005460},
	author = {Baumberger, T and Lilley, M D and Resing, J A and Lupton, J E and Baker, E T and Butterfield, D A and Olson, E J and Fruh-Green, G L}
}
@article {Tucholke2013,
	title = {{Cemented mounds and hydrothermal sediments on the detachment surface at Kane Megamullion: A new manifestation of hydrothermal venting}},
	journal = {Geochemistry, Geophysics, Geosystems},
	volume = {14},
	year = {2013},
	pages = {3352{\textendash}3378},
	abstract = {Long-lived detachment faults are now known to be important in tectonic evolution of slow-spreading mid-ocean ridges, and there is increasing evidence that fluid flow plays a critical role in development of detachment systems. Here we document a new manifestation of low-temperature hydrothermal venting associated with the detachment fault that formed Kane Megamullion \~{}3.3{\textendash}2.1 m.y. ago in the western rift-valley wall of the Mid-Atlantic Ridge. Hydrothermal effects on the detachment surface include (1) cemented mounds of igneous rock and chalk debris containing hydrothermal Mn oxides and Fe oxyhydroxides, and (2) layered deposits of similar Fe-Mn minerals {\textpm} interbedded chalks. Mounds are roughly conical, \~{}1{\textendash}10 m high, and contain primarily basalts with lesser gabbro, serpentinite, and polymict breccia. The layered Fe-Mn-rich sediments are flat-bedded to contorted and locally are buckled into low-relief linear or polygonal ridges. We propose that the mounds formed where hydrothermal fluids discharged through the detachment hanging wall near the active fault trace. Hydrothermal precipitates cemented hanging-wall debris and welded it to the footwall, and this debris persisted as mounds as the footwall was exhumed and surrounding unconsolidated material sloughed off the sloping detachment surface. Some of the layered Fe-Mn-rich deposits may have precipitated from fluids discharging from the hanging-wall vents, but they also precipitated from low-temperature fluids venting from the exposed footwall through overlying chalks. Observed natural disturbance and abnormally thin hydrogenous Fe-Mn crusts on some contorted, hydrothermal Fe-Mn-rich chalks on \~{}2.7 Ma crust suggest diffuse venting that is geologically recent. Results of this study imply that there are significant fluid pathways through all parts of detachment systems and that low-temperature venting through fractured detachment footwalls may continue for several million years off-axis.},
	keywords = {AUV ABE (Autonomous Benthic Explorer), ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1002/ggge.20186},
	author = {Tucholke, B E and Humphris, S E and Dick, H J B}
}
@article {Jacobson2013a,
	title = {{Characterization of 13 polymorphic microsatellite loci in Rimicaris hybisae, a shrimp from deep-sea hydrothermal vents}},
	journal = {Conservation Genetics Resources},
	volume = {5},
	number = {2},
	year = {2013},
	month = {jun},
	pages = {449{\textendash}451},
	abstract = {Rimicaris hybisae is a deep-sea alvinocaridid shrimp found at hydrothermal vents along the Mid-Cayman Spreading Center. Eleven selectively neutral and unlinked polymorphic microsatellite loci were developed for this species and two additional loci were found to cross-amplify from a related species. Nine loci conformed to Hardy-Weinberg expectations. Seven loci cross-amplified with Chorocaris sp. 2, an alvinocaridid shrimp found at vents in the Southwestern Pacific. Microsatellite loci developed for R. hybisae are being deployed to study connectivity and genetic variability of populations along the Mid-Cayman Spreading Center.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1877-7252},
	doi = {10.1007/s12686-012-9825-0},
	author = {Jacobson, A and Plouviez, S and Thaler, A D and Van Dover, C L}
}
@article {Jacobson2013,
	title = {{Characterization of 9 polymorphic microsatellite loci in Lamellibrachia sp 2, a tubeworm found at deep-sea hydrothermal vents and cold seeps}},
	journal = {Conservation Genetics Resources},
	volume = {5},
	number = {4},
	year = {2013},
	month = {dec},
	pages = {1005{\textendash}1007},
	abstract = {Lamellibrachia sp. 2 is a deep-sea vestimentiferan tubeworm found at hydrothermal vents on the Mid-Cayman Spreading Center and at hydrocarbon seeps in the Gulf of Mexico and off the coast of Barbados. Nine selectively neutral and unlinked polymorphic microsatellite loci were developed for this species. Eight of these loci conformed to Hardy-Weinberg expectations. Average observed heterozygosity ranged from 0.14 to 0.92. Microsatellites developed for Lamellibrachia sp. 2 are being deployed to study connectivity and gene flow among populations of this species.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1877-7252},
	doi = {10.1007/s12686-013-9955-z},
	author = {Jacobson, A and Plouviez, S and Thaler, A D and Van Dover, C L}
}
@article {Feng2013,
	title = {{A comparative study of authigenic carbonates from mussel and tubeworm environments: Implications for discriminating the effects of tubeworms}},
	journal = {Deep-Sea Research. Part I: Oceanographic Research Papers},
	volume = {75},
	year = {2013},
	pages = {110{\textendash}118},
	abstract = {The Gulf of Mexico hydrocarbon seeps are often populated by dense mussel beds and tubeworm aggregations, as well as exposed authigenic carbonate outcrops. Previous studies suggest the activity of mussels and tubeworms could influence the sediment geochemistry of their habitats, resulting in variations in the stable carbon isotopes of the associated carbonates. However, this conclusion was based on the analyses of samples from a single site. To better understand whether there are consistent differences in the geochemical environments of mussels and tubeworms, mineralogical and stable isotopic compositions of authigenic carbonates from mussel and tubeworm environments from four seep sites were analyzed. The studied sites span a depth range of 1200~m to 2800~m on the northern Gulf of Mexico continental slope. We found that carbonate samples from tubeworm environments were more prone to contain aragonite whereas carbonates from mussel environments were more likely to have calcite. This finding supports the hypothesis that vestimentiferans release sulfate across their roots into the pore waters of the surrounding sediments, a process that could generate a locally sulfate-enriched environment that favors the precipitation of aragonite instead of calcite. Moreover, the $δ$13C values of tubeworm carbonates are generally lighter than that of mussel carbonates from the same site, which is consistent with the fact that tubeworms are fueling extra subsurface methane oxidation through the release of sulfate into the sediment. Such a process, consequently, enriches the subsurface dissolved inorganic carbon pool with light carbon derived from the seeping hydrocarbons. Taken together, our data suggest that tubeworms could produce a carbon isotope shift that is sufficient to influence the sediment geochemistry of their immediate area, and that this impact is reflected in the associated authigenic carbonates.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0637},
	doi = {10.1016/j.dsr.2013.02.002},
	url = {http://www.sciencedirect.com/science/article/pii/S0967063713000435},
	author = {Feng, D and Cordes, E E and Roberts, H H and Fisher, C R}
}
@article {Sen2013,
	title = {{Distribution of mega fauna on sulfide edifices on the Eastern Lau Spreading Center and Valu Fa Ridge}},
	journal = {Deep-Sea Research. Part I: Oceanographic Research Papers},
	volume = {72},
	year = {2013},
	pages = {48{\textendash}60},
	abstract = {Hydrothermal vent sulfide edifices contain some of the most extreme thermal and chemical conditions in which animals are able to live. As a result, sulfide edifices in the East Pacific Rise, Juan de Fuca Ridge, and Mid Atlantic Ridge vent systems often contain distinct faunal assemblages. In this study, we used high-resolution imagery and in-situ physico-chemical measurements within the context of a Geographic Information System (GIS) to examine community structure and niche differentiation of dominant fauna on sulfide edifices in the Eastern Lau Spreading Center (ELSC) and Valu Fa Ridge (VFR) in the Western Pacific Ocean. Our results show that ELSC and VFR sulfide edifices host two distinct types of communities. One type, that covers the majority of sulfide edifice faces, is overall very similar to nearby lava communities and biomass is dominated by the same chemoautotrophic symbiont-containing molluscs that dominate lava communities, namely the provannid gastropods Alviniconcha spp. and Ifremeria nautilei and the mytilid bivalve Bathymodiolus brevior. The spatial distribution of the dominant molluscs is often a variation of the pattern of concentric rings observed on lavas, with Alviniconcha spp. at the tops of edifices where exposure to vent flow is the highest, and I. nautilei and B. brevior below. Our physico-chemical measurements indicate that because of rapid dispersion of vent fluid, habitable area for symbiont-containing fauna is quite limited on sulfide edifices, and the realized niches of the mollusc groups are narrower on sulfide edifices than on lavas. We suggest that competition plays an important role in determining the realized distributions of the mollusc groups on edifices. The other habitat, present in small patches of presumably hot, new anhydrite, is avoided by the dominant symbiont-containing molluscs and inhabited by crabs, shrimp and polynoids that are likely more heat tolerant. The ratio of sulfide concentration to temperature anomaly of vent fluids was significantly different between sulfide edifice sites and lava sites in the southern vent fields but not in the northern vent fields. We suggest that this is due to increased sulfide consumption by a large microbial consortium associated with the more friable andesitic lava substrates in the south.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0637},
	doi = {10.1016/j.dsr.2012.11.003},
	url = {http://www.sciencedirect.com/science/article/pii/S0967063712002233},
	author = {Sen, A and Becker, E L and Podowski, E L and Wickes, L N and Ma, S and Mullaugh, K M and Hourdez, S and Luther, G W and Fisher, C R}
}
@article {Levin2013,
	title = {{Ecological release and niche partitioning under stress:lessons from dorvilleid polychaetes in sulfidic sediments at methane seeps}},
	journal = {Deep-Sea Research. Part II: Topical Studies in Oceanography},
	volume = {92},
	year = {2013},
	pages = {214{\textendash}233},
	abstract = {Organisms inhabiting methane seep sediments are exposed to stress in the form of high levels of hydrogen sulfide, which result mainly from sulfate reduction coupled to anaerobic methane oxidation. Dorvilleidae (Polychaeta) have successfully invaded this ecosystem, and multiple species in divergent genetic clades co-occur at high densities. At methane seeps in the NE Pacific off California and Oregon, the genera Ophryotrocha, Parougia and Exallopus are especially well represented. To test the hypothesis that dorvilleid coexistence is facilitated by niche partitioning through sulfide tolerance and trophic patterns, we examined dorvilleid species-specific patterns of occurrence and nutrition at methane seeps off Eel R. [ER] on the Californian continental slope and at Hydrate Ridge [HR] on the Oregon continental slope, and in two habitats (clam bed and microbial mat) characterized by lower and higher hydrogen sulfide levels, respectively. Microelectrode measurements of hydrogen sulfide enabled characterization of environmental sulfide levels for species sampled in background sediment cores and in colonization trays. Dorvilleids tolerated H2S levels from 10~$μ$M to over 2.6~mM, with the majority of species inhabiting sediments with similar environmental H2S concentrations (median 85{\textendash}100~$μ$M). Dorvilleid species richness was greater at HR than ER, but did not differ between clam bed and microbial mat habitats. Species distribution patterns reflected preferences for ER clam bed (lower sulfide levels), ER mat and HR clam bed (moderate sulfide levels), or HR mat (very high sulfide levels). Nutritional patterns, including trophic diversity and functional similarity, were examined using community stable isotope metrics based on $δ$15N and $δ$13C. Within each region, dorvilleid species exhibited multiple trophic strategies. Co-existing congeners typically exhibited distinct isotope signatures, suggesting trophic partitioning. Trophic diversity and $δ$15N range for whole assemblages (measured by Total Hull Area and Standard Elliptical Area using species averages) and functional redundancy or species packing (measured as distance to nearest neighbor) among species and individuals were generally higher at ER, where sulfide levels were lower than at HR. In contrast, average trophic diversity among individuals within a species was greater at HR than ER. In colonization experiments involving agar-based manipulations of sulfide in tray sediments that mimicked clam bed and mat conditions, dorvilleids comprised 68{\%} and 48{\%} of colonists at ER and HR, respectively. Dorvilleid species richness was higher in trays that were initially more sulfidic. However, habitat exerted stronger influence on the composition of colonizing dorvilleids than did sulfide additions. In the NE Pacific, regional, habitat and vertical (down-core) variation in hydrogen sulfide creates complex environmental heterogeneity at methane seeps, promoting high diversity of stress-tolerant taxa such as dorvilleid polychaetes.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0645},
	doi = {10.1016/j.dsr2.2013.02.006},
	url = {http://www.sciencedirect.com/science/article/pii/S0967064513000635},
	author = {Levin, L A and Ziebis, W and Mendoza, G and Bertics, V J and Washington, T and Gonzalez, J and Thurber, A R and Ebbe, B and Lee, R W}
}
@article {ISI:000331005900001,
	title = {{Low temperature geomicrobiology follows host rock composition along a geochemical gradient in Lau Basin}},
	journal = {FRONTIERS IN MICROBIOLOGY},
	volume = {4},
	year = {2013},
	month = {mar},
	type = {Article},
	abstract = {The East Lau Spreading Center (ELSC) and Valu Fa Ridge (VFR) comprise a ridge segment in the southwest Pacific Ocean where rapid transitions in the underlying mantle chemistry manifest themselves as gradients in seafloor rock geochemistry. We studied the geology and microbial diversity of three silicate rock samples and three inactive sulfide chimney samples collected, from north to south, at the vent fields Kilo Moana, ABE, Tui Malila, and Mariner. This is the first study of microbial populations on basaltic andesite, which was sampled at Mariner vent field. Silicate rock geochemistry exhibits clear latitudinal trends that are mirrored by changes in bacterial community composition. alpha-proteobacteria, epsilon-proteobacteria, and Bacteroidetes are most common on a silicate collected from Kilo Moana and their proportions decrease linearly on silicates collected further south. Conversely, a silicate from Mariner vent field hosts high proportions of a unique lineage of Chloroflexi unrelated ({\textless}90{\%} sequence similarity) to previously recovered environmental clones or isolates, which decrease at ABE and are absent at Kilo Moana. The exteriors of inactive sulfide structures are dominated by lineages of sulfur oxidizing alpha-proteobacteria, gamma-proteobacteria, and epsilon-proteobacteria, while the interior of one chimney is dominated by putative sulfur-reducing delta-proteobacteria. A comparison of bacterial communities on inactive sulfides from this and previous studies reveals the presence of a clade of uncultured Bacteroidetes exclusive to sulfidic environments, and a high degree of heterogeneity in bacterial community composition from one sulfide structure to another. In light of the heterogeneous nature of bacterial communities observed here and in previous studies of both active and inactive hydrothermal sulfide structures, the presence of numerous niches may be detected on these structures in the future by finer scale sampling and analysis.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1664-302X},
	doi = {10.3389/fmicb.2013.00061},
	author = {Sylvan, Jason B and Sia, Tiffany Y and Haddad, Amanda G and Briscoe, Lindsey J and Toner, Brandy M and Girguis, Peter R and Edwards, Katrina J}
}
@article {ISI:000323828800015,
	title = {{A low-relief shield volcano origin for the South Kaua{\{}{\textquoteright}{\textquoteright}{\}}i Swell}},
	journal = {GEOCHEMISTRY GEOPHYSICS GEOSYSTEMS},
	volume = {14},
	number = {7},
	year = {2013},
	month = {jul},
	pages = {2328{\textendash}2348},
	type = {Article},
	abstract = {The South Kauai Swell (SKS) is a 110 km x 80 km ovoid bathymetric feature that stands {\textgreater}2 km high and abuts the southern flank of the island of Kauai. The origin of the SKS was investigated using multibeam bathymetry and acoustic backscatter, gravity data, radiometric ages, and geochemistry of rock samples. Most of the SKS rock samples are tholeiitic in composition with ages of 3.9-5.4 Ma indicating they were derived from shield volcanism. The ages and compositions of the SKS rocks partially overlap with those of the nearby Niihau, Kauai and West Kaena volcano complexes. The SKS was originally described as a landslide; however, this interpretation is problematic given the ovoid shape of SKS, its relatively smooth, flat-to-convex surface, and the lack of an obvious source region that could accommodate what would be one of Earth{\textquoteright}s most voluminous (6 x 10(3) km(3)) landslides. The morphology, size, and the surrounding gravity anomaly are more consistent with the SKS being a low-relief shield volcano, which was partially covered with a small volume of landside debris from south Kauai and later with some secondary volcanic seamounts. A shield origin would imply that Hawaiian and possibly other hotspot shield volcanoes can take on a wider variety of forms than is commonly thought, ranging from tall island-building shields, to smaller edifices such as Kaena Ridge and Mahukona, to even lower-relief volcanoes represented by the SKS and possibly the South West Oahu Volcanic Field.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1525-2027},
	doi = {10.1002/ggge.20159},
	author = {Ito, Garrett and Garcia, Michael O and Smith, John R and Taylor, Brian and Flinders, Ashton and Jicha, Brian and Yamasaki, Seiko and Weis, Dominique and Swinnard, Lisa and Blay, Chuck}
}
@article {ISI:000322119600009,
	title = {{Metatranscriptomics reveal differences in in situ energy and nitrogen metabolism among hydrothermal vent snail symbionts}},
	journal = {ISME JOURNAL},
	volume = {7},
	number = {8},
	year = {2013},
	month = {aug},
	pages = {1556{\textendash}1567},
	type = {Article},
	abstract = {Despite the ubiquity of chemoautotrophic symbioses at hydrothermal vents, our understanding of the influence of environmental chemistry on symbiont metabolism is limited. Transcriptomic analyses are useful for linking physiological poise to environmental conditions, but recovering samples from the deep sea is challenging, as the long recovery times can change expression profiles before preservation. Here, we present a novel, in situ RNA sampling and preservation device, which we used to compare the symbiont metatranscriptomes associated with Alviniconcha, a genus of vent snail, in which specific host-symbiont combinations are predictably distributed across a regional geochemical gradient. Metatranscriptomes of these symbionts reveal key differences in energy and nitrogen metabolism relating to both environmental chemistry (that is, the relative expression of genes) and symbiont phylogeny (that is, the specific pathways employed). Unexpectedly, dramatic differences in expression of transposases and flagellar genes suggest that different symbiont types may also have distinct life histories. These data further our understanding of these symbionts{\textquoteright} metabolic capabilities and their expression in situ, and suggest an important role for symbionts in mediating their hosts{\textquoteright} interaction with regional-scale differences in geochemistry.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1751-7362},
	doi = {10.1038/ismej.2013.45},
	author = {Sanders, J G and Beinart, R A and Stewart, F J and Delong, E F and Girguis, P R}
}
@article {Joseph2013,
	title = {{Methane-derived authigenic carbonates from modern and paleoseeps on the Cascadia margin: Mechanisms of formation and diagenetic signals}},
	journal = {Tracing Phanerozoic hydrocarbon seepage from local basins to the global Earth system},
	volume = {390},
	year = {2013},
	pages = {52{\textendash}67},
	abstract = {Authigenic carbonate precipitation occurs within marine sediments where sulfate-dependent anaerobic methane oxidation occurs. Geochemical and isotopic analyses of authigenic carbonates are commonly used as indicators of carbon sources and environmental conditions present during carbonate formation, but burial diagenesis and recrystallization can overprint these signals. Plane polarized light (PPL) and cathodoluminescent (CL) petrography allows for detailed characterization of carbonate phases and their subsequent alteration. Petrographic, isotopic, and geochemical characteristics of modern offshore authigenic carbonates from central and northern Cascadia are compared with Oligocene{\textendash}Pliocene fossil seep carbonates uplifted on the Olympic Peninsula. Coupled analyses show the value and complexity of separating primary vs. secondary signals with relevance to understanding fluid-burial history in methane seep provinces on tectonically active convergent margins. The modern, offshore, near-seafloor diagenetic environment (S. Hydrate Ridge and Barkley Canyon) is dominated by acicular and microcrystalline aragonite and high-Mg calcite (HMC, {\textgreater}~12~mol {\%} Mg). PPL and CL data illustrate that aragonite and HMC phases recrystallize to intermediate-Mg calcite (IMC, 5{\textendash}12~mol{\%} Mg) during burial and diagenesis and eventually to low-Mg calcite (LMC, 18O attributable to elevated fluid temperatures during recrystallization.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0031-0182},
	doi = {10.1016/j.palaeo.2013.01.012},
	url = {http://www.sciencedirect.com/science/article/pii/S0031018213000333},
	author = {Joseph, C and Campbell, K A and Torres, M E and Martin, R A and Pohlman, J W and Riedel, M and Rose, K}
}
@article {ISI:000313236000014,
	title = {{Microbial diversity within basement fluids of the sediment-buried Juan de Fuca Ridge flank}},
	journal = {ISME JOURNAL},
	volume = {7},
	number = {1},
	year = {2013},
	month = {jan},
	pages = {161{\textendash}172},
	type = {Article},
	abstract = {Despite its immense size, logistical and methodological constraints have largely limited microbiological investigations of the subseafloor basement biosphere. In this study, a unique sampling system was used to collect fluids from the subseafloor basaltic crust via a Circulation Obviation Retrofit Kit (CORK) observatory at Integrated Ocean Drilling Program borehole 1301A, located at a depth of 2667 m in the Pacific Ocean on the eastern flank of the Juan de Fuca Ridge. Here, a fluid delivery line directly accesses a 3.5 million years old basalt-hosted basement aquifer, overlaid by 262 m of sediment, which serves as a barrier to direct exchange with bottom seawater. At an average of 1.2 x 10(4) cells ml(-1), microorganisms in borehole fluids were nearly an order of magnitude less abundant than in surrounding bottom seawater. Ribosomal RNA genes were characterized from basement fluids, providing the first snapshots of microbial community structure using a high-integrity fluid delivery line. Interestingly, microbial communities retrieved from different CORKs (1026B and 1301A) nearly a decade apart shared major community members, consistent with hydrogeological connectivity. However, over three sampling years, the dominant gene clone lineage changed from relatives of Candidatus Desulforudis audaxviator within the bacterial phylum Firmicutes in 2008 to the Miscellaneous Crenarchaeotic Group in 2009 and a lineage within the JTB35 group of Gammaproteobacteria in 2010, and statistically significant variation in microbial community structure was observed. The enumeration of different phylogenetic groups of cells within borehole 1301A fluids supported our observation that the deep subsurface microbial community was temporally dynamic. The ISME Journal (2013) 7, 161-172; doi:10.1038/ismej.2012.73; published online 12 July 2012},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {1751-7362},
	doi = {10.1038/ismej.2012.73},
	author = {Jungbluth, Sean P and Grote, Jana and Lin, Huei-Ting and Cowen, James P and Rappe, Michael S}
}
@article {Meyer2013,
	title = {{Microbiological characterization of post-eruption {\textquotedblleft}snowblower{\textquotedblright} vents at Axial Seamount, Juan de Fuca Ridge}},
	journal = {Frontiers in Microbiology},
	volume = {4},
	year = {2013},
	pages = {153},
	abstract = {Microbial processes within the subseafloor can be examined during the ephemeral and uncommonly observed phenomena known as snowblower venting. Snowblowers are characterized by the large quantity of white floc that is expelled from the seafloor following mid-ocean ridge eruptions. During these eruptions, rapidly cooling lava entrains seawater and hydrothermal fluids enriched in geochemical reactants, creating a natural bioreactor that supports a subseafloor microbial {\textquotedblleft}bloom.{\textquotedblright} Previous studies hypothesized that the eruption-associated floc was made by sulfide-oxidizing bacteria; however, the microbes involved were never identified. Here we present the first molecular analysis combined with microscopy of microbial communities in snowblower vents from samples collected shortly after the 2011 eruption at Axial Seamount, an active volcano on the Juan de Fuca Ridge. We obtained fluid samples and white flocculent material from active snowblower vents as well as orange flocculent material found on top of newly formed lava flows. Both flocculent types revealed diverse cell types and particulates when examined by phase contrast and scanning electron microscopy (SEM). Distinct archaeal and bacterial communities were detected in each sample type through Illumina tag sequencing of 16S rRNA genes and through sequencing of the sulfide oxidation gene, soxB. In fluids and white floc, the dominant bacteria were sulfur-oxidizing Epsilonproteobacteria and the dominant archaea were thermophilic Methanococcales. In contrast, the dominant organisms in the orange floc were Gammaproteobacteria and Thaumarchaeota Marine Group I. In all samples, bacteria greatly outnumbered archaea. The presence of anaerobic methanogens and microaerobic Epsilonproteobacteria in snowblower communities provides evidence that these blooms are seeded by subseafloor microbes, rather than from microbes in bottom seawater. These eruptive events thus provide a unique opportunity to observe subseafloor microbial communities.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.3389/fmicb.2013.00153},
	author = {Meyer, J L and Akerman, N H and Proskurowski, G and Huber, J A}
}
@article {ISI:000323108100003,
	title = {{Morphology, microanatomy and sequence data of Sclerolinum contortum (Siboglindae, Annelida) of the Gulf of Mexico}},
	journal = {ORGANISMS DIVERSITY {\&} EVOLUTION},
	volume = {13},
	number = {3},
	year = {2013},
	month = {sep},
	pages = {311{\textendash}329},
	type = {Article},
	abstract = {Sclerolinum is a small genus of Siboglinidae (Annelida) living in an obligate mutualistic association with thiotrophic bacteria as adults. Its taxonomic position, based on morphology, has been controversial; however, molecular data point to a sister taxa relationship with vestimentiferans. 16S rRNA gene sequencing and comparative morphology revealed that the studied population from deep-sea hydrocarbon seeps of the Gulf of Mexico belongs to Sclerolinum contortum known from the Arctic Sea. Since no anatomical and microanatomical studies have been published yet, we conducted such a study on S. contortum using serial sectioning and light and transmission electron microscopy. We show that the Sclerolinum body, divided into a head, trunk, and opisthosoma, is very similar to that of the vestimentiferans, and therefore we propose that the body regions are homologous in both taxa.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1439-6092},
	doi = {10.1007/s13127-012-0121-3},
	author = {Eichinger, Irmgard and Hourdez, Stephane and Bright, Monika}
}
@article {Hansen2013,
	title = {{Mylonitic deformation at the Kane oceanic core complex: Implications for the rheological behavior of oceanic detachment faults}},
	journal = {Geochemistry, Geophysics, Geosystems},
	volume = {14},
	year = {2013},
	pages = {3085{\textendash}3108},
	abstract = {The depth extent, strength, and composition of oceanic detachment faults remain poorly understood because the grade of deformation-related fabrics varies widely among sampled oceanic core complexes (OCCs). We address this issue by analyzing fault rocks collected from the Kane oceanic core complex at 23{\textdegree}30'N on the Mid-Atlantic Ridge. A portion of the sample suite was collected from a younger fault scarp that cuts the detachment surface and exposes the interior of the most prominent dome. The style of deformation was assessed as a function of proximity to the detachment surface, revealing a \~{}450 m thick zone of high-temperature mylonitization overprinted by a \~{}200 m thick zone of brittle deformation. Geothermometry of deformed gabbros demonstrates that crystal-plastic deformation occurred at temperatures {\textgreater}700{\textdegree}C. Analysis of the morphology of the complex in conjunction with recent thermochronology suggests that deformation initiated at depths of \~{}7 km. Thus we suggest the detachment system extended into or below the brittle-plastic transition (BPT). Microstructural evidence suggests that gabbros and peridotites with high-temperature fabrics were dominantly deforming by dislocation-accommodated processes and diffusion creep. Recrystallized grain size piezometry yields differential stresses consistent with those predicted by dry-plagioclase flow laws. The temperature and stress at the BPT determined from laboratory-derived constitutive models agree well with the lowest temperatures and highest stresses estimated from gabbro mylonites. We suggest that the variation in abundance of mylonites among oceanic core complexes can be explained by variation in the depth of the BPT, which depends to a first order on the thermal structure and water content of newly forming oceanic lithosphere.},
	keywords = {AUV ABE (Autonomous Benthic Explorer), ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1002/ggge.20184},
	author = {Hansen, L N and Cheadle, M J and John, B E and Swapp, S M and Dick, H J B and Tucholke, B E and Tivey, M A}
}
@article {Reiswig2013,
	title = {{New glass sponges (Porifera: Hexactinellida) from deep waters of the central Aleutian Islands, Alaska}},
	journal = {Zootaxa},
	volume = {3628},
	year = {2013},
	pages = {1{\textendash}64},
	abstract = {Hexactinellida from deep-water communities of the central Aleutian Islands, Alaska, are described. They were mostly collected by the remotely operated vehicle {\textquoteright}Jason II{\textquoteright} from 494{\textendash}2311 m depths during a 2004 RV {\textquoteright}Roger Revelle{\textquoteright} expedition, but one shallow-water species collected with a shrimp trawl from 155 m in the same area is included. The excellent condition of the ROV-collected specimens enabled valuable redescription of some species previously known only from badly damaged specimens. New taxa include one new genus and eight new species in five families. Farreidae consist of two new species, Farrea aleutiana and F. aspondyla. Euretidae consists of only Pinulasma fistulosum n. gen., n. sp. Tretodictyidae include only Tretodictyum amchitkensis n. sp. Euplectellidae consists of only the widespread species Regadrella okinoseana Ijima, reported here over 3,700 km from its closest previously known occurrence. The most diverse family, Rossellidae, consists of Aulosaccus ijimai (Schulze), Aulosaccus schulzei Ijima, Bathydorus sp. (young stage not determinable to species), Caulophacus (Caulophacus) adakensis n. sp., Acanthascus koltuni n. sp., Staurocalyptus psilosus n. sp., Staurocalyptus tylotus n. sp. and Rhabdocalyptus mirabilis Schulze. We present argument for reinstatement of the abolished rossellid subfamily Acanthascinae and return of the subgenera Staurocalyptus Ijima and Rhabdocalyptus Schulze to their previous generic status. These fauna provides important complexity to the hard substrate communities that likely serve as nursery areas for the young stages of commercially important fish and crab species, refuge from predation for both young and adult stages, and also as a focal source of prey for juvenile and adult stages of those same species.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.11646/zootaxa.3628.1.1},
	author = {Reiswig, H M and Stone, R P}
}
@article {Nye2013,
	title = {{A new species of Lebbeus (Crustacea: Decapoda: Caridea: Hippolytidae) from the Von Damm Vent Field, Caribbean Sea}},
	journal = {Journal of the Marine Biological Association of the United Kingdom},
	volume = {93},
	number = {3},
	year = {2013},
	month = {may},
	pages = {741{\textendash}751},
	abstract = {A new species of the hippolytid shrimp genus Lebbeus White, 1847 is described from the Von Damm Vent Field (VDVF) on the Mid-Cayman Spreading Centre, Caribbean Sea, at 2294 m water depth. Lebbeus virentova sp. nov. is defined and illustrated from seven specimens, with brief notes on its distribution and habitat. Molecular phylogenetic data from the COI mDNA region are used to analyse the species{\textquoteright} phylogenetic position, and its morphology is compared with previously described species. This new species represents the second family of caridean shrimp to be reported from the VDVF. Lebbeus virentova sp. nov. is the eighth member of the genus to be described from hydrothermal vents and appears to be the first hippolytid shrimp at a vent field known from outside the Pacific Ocean.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0025-3154},
	doi = {10.1017/S0025315412000884},
	author = {Nye, Verity and Copley, J and Plouviez, S and Van Dover, C L}
}
@article {ISI:000322229800020,
	title = {{Niche divergence by deep-sea octocorals in the genus Callogorgia across the continental slope of the Gulf of Mexico}},
	journal = {MOLECULAR ECOLOGY},
	volume = {22},
	number = {15},
	year = {2013},
	month = {aug},
	pages = {4123{\textendash}4140},
	type = {Article},
	abstract = {Environmental variables that are correlated with depth have been suggested to be among the major forces underlying speciation in the deep sea. This study incorporated phylogenetics and ecological niche models (ENM) to examine whether congeneric species of Callogorgia (Octocorallia: Primnoidae) occupy different ecological niches across the continental slope of the Gulf of Mexico (GoM) and whether this niche divergence could be important in the evolution of these closely related species. Callogorgia americana americana, Callogorgia americana delta and Callogorgia gracilis were documented at 13 sites in the GoM (250-1000 m) from specimen collections and extensive video observations. On a first order, these species were separated by depth, with C. gracilis occurring at the shallowest sites, C. a. americana at mid-depths and C. a. delta at the deepest sites. Callogorgia a. delta was associated with areas of increased seep activity, whereas C. gracilis and C. a. americana were associated with narrow, yet warmer, temperature ranges and did not occur near cold seeps. ENM background and identity tests revealed little to no overlap in ecological niches between species. Temporal calibration of the phylogeny revealed the formation of the Isthmus of Panama was a vicariance event that may explain some of the patterns of speciation within this genus. These results elucidate the potential mechanisms for speciation in the deep sea, emphasizing both bathymetric speciation and vicariance events in the evolution of a genus across multiple regions.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0962-1083},
	doi = {10.1111/mec.12370},
	author = {Quattrini, Andrea M and Georgian, Samuel E and Byrnes, Luke and Stevens, Alex and Falco, Rosalia and Cordes, Erik E}
}
@article {ISI:000331246100002,
	title = {{Phylogenetic diversity and functional gene patterns of sulfur-oxidizing subseafloor Epsilonproteobacteria in diffuse hydrothermal vent fluids}},
	journal = {FRONTIERS IN MICROBIOLOGY},
	volume = {4},
	year = {2013},
	month = {jul},
	type = {Article},
	abstract = {Microorganisms throughout the dark ocean use reduced sulfur compounds for chemolithoautotrophy. In many deep-sea hydrothermal vents, sulfide oxidation is quantitatively the most important chemical energy source for microbial metabolism both at and beneath the seafloor. In this study, the presence and activity of vent endemic Epsilonproteobacteria was examined in six low-temperature diffuse vents over a range of geochemical gradients from Axial Seamount, a deep-sea volcano in the Northeast Pacific. PCR primers were developed and applied to target the sulfur oxidation soxB gene of Epsilonproteobacteria. soxB genes belonging to the genera Sulfurimonas and Sulfurovum are both present and expressed at most diffuse vent sites, but not in background seawater. Although Sulfurovum-like sox8 genes were detected in all fluid samples, the RNA profiles were nearly identical among the vents and suggest that Sulfurirnonas-like species are the primary Epsilonproteobacteria responsible for actively oxidizing sulfur via the Sox pathway at each vent. Community patterns of subseafloor Epsdonproteobactena 16S rRNA genes were best matched to methane concentrations in vent fluids, as well as individual vent locations, indicating that both geochemistry and geographical isolation play a role in structuring subseafloor microbial populations. The data show that in the subseafloor at Axial Seamount, Epsilonproteobacteria are expressing the soxB gene and that microbial patterns in community distribution are linked to both vent location and chemistry.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1664-302X},
	doi = {10.3389/fmicb.2013.00185},
	author = {Akerman, Nancy H and Butterfield, David A and Huber, Julie A}
}
@article {Tunnicliffe2013,
	title = {{Population ecology of the tonguefish Symphurus thermophilus (Pisces; Pleuronectiformes; Cynoglossidae) at sulphur-rich hydrothermal vents on volcanoes of the northern Mariana Arc}},
	journal = {Deep-Sea Research. Part II: Topical Studies in Oceanography},
	volume = {92},
	year = {2013},
	pages = {172{\textendash}182},
	abstract = {Flatfish are a major component of the hydrothermal vent community on three seamounts of the northern Mariana Volcanic Arc in the northwest Pacific. Nikko, Kasuga-2 and Daikoku seamounts host vent fields between 375 and 480~m depth where high temperature vents release molten sulphur. The small cynoglossid tonguefish, Symphurus thermophilus Munroe and Hashimoto, is ubiquitous in all vent habitats observed on these seamounts: among extensive fields of tubeworms and mussels and on solid sulphur surfaces on Nikko; on sulphur-rich sediments and barnacle-covered boulders on Kasuga-2; and on recent sulphur flows and on broad areas of loose and semi-consolidated sediments on Daikoku. We recorded repeated forays by individuals onto flows of molten sulphur as these surfaces cooled. Based on observations using ROVs, the mean density is 90~fish/m2 with maximum counts over 200~fish/m2 on Daikoku sediments. Compared to collected tonguefish from Daikoku and Kasuga-2, those from Nikko have significantly greater lengths and, on average, six times the mass. Otolith data indicate upper ages of 13 years with Nikko tonguefish growing significantly faster. Diets of tonguefish on the three seamounts reflect the different habitats and prey availability; in Daikoku specimens, small crustaceans and polychaetes are most common while on Nikko, gut contents are predominantly larger shrimp. We made the unusual observation of stunned midwater fish falling to the seafloor near the vents where S. thermophilus immediately attacked them. This tonguefish has a wide diet range and foraging behaviour that likely influence the differing growth rates and sizes of fish inhabiting the different vent sites. Limited genetic data suggest that larval exchange probably occurs among sites where the common habitat factor is high levels of elemental sulphur forming hard and partly unconsolidated substrata. Here, in the northern range of the Mariana Trench Marine National Monument, S. thermophilus, despite having an unusually broad habitat association, may be restricted in its overall range to this region of isolated volcanoes with active hydrothermalism.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0645},
	doi = {10.1016/j.dsr2.2013.01.026},
	url = {http://www.sciencedirect.com/science/article/pii/S0967064513000465},
	author = {Tunnicliffe, V and Tyler, J and Dower, J F}
}
@article {Waters2013,
	title = {{Sill to surface: Linking young off-axis volcanism with subsurface melt at the overlapping spreading center at 9{\textdegree}03'N East Pacific Rise}},
	journal = {Earth and Planetary Science Letters},
	volume = {369{\textendash}370},
	year = {2013},
	pages = {59{\textendash}70},
	abstract = {No young, off-axis, mid-ocean ridge lavas have yet been directly linked to underlying off-axis melt bodies. In this study, we present new measurements of 238U{\textendash}230Th{\textendash}226Ra{\textendash}210Pb isotope compositions for a suite of lavas from the overlapping spreading center (OSC) at 9{\textdegree}03'N on the East Pacific Rise (EPR). These lavas span a large range of compositions, from basalt to dacite, and include both axial and off-axis samples recovered from a prominent, axis-parallel pillow ridge and a flat-topped seamount that overlie the westernmost extent of a 4-km-wide melt lens (Kent et al., 2000). We report 210Pb excesses in axial basalts and basaltic andesites, which we suggest results from gas-magma fractionation of 222Rn from 226Ra beneath dacite magmas. In addition, our U-series ages agree with visual observations, indicating that while most recent volcanic activity occurs at the spreading axis, active volcanism also occurs away from the axis. Specifically, the off-axis pillow ridge and seamount samples overlying the off-axis subsurface melt body have eruption ages of less than 8~ka, and likely as young as 1~ka, despite being located on crust that has a spreading age of {\~{}}75~ka. The young ages of these lavas, combined with existing geological, geochemical and geophysical constraints, provide evidence for a genetic link between the pillow ridge and seamount lavas and the seismically imaged, underlying off-axis melt lens. This link demonstrates that off-axis volcanism does not necessarily come from a sub-axial magma body and can be sourced directly from off-axis magma bodies.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0012-821X},
	doi = {10.1016/j.epsl.2013.03.006},
	url = {http://www.sciencedirect.com/science/article/pii/S0012821X13001209},
	author = {Waters, C L and Sims, K W W and Klein, E M and White, S M and Reagan, M K and Girard, G}
}
@article {ISI:000326247700007,
	title = {{Surficial permeability of the axial valley seafloor: Endeavour Segment, Juan de Fuca Ridge}},
	journal = {GEOCHEMISTRY GEOPHYSICS GEOSYSTEMS},
	volume = {14},
	number = {9},
	year = {2013},
	month = {sep},
	pages = {3409{\textendash}3424},
	type = {Article},
	abstract = {Hydrothermal systems at mid-ocean spreading centers play a fundamental role in Earth{\textquoteright}s geothermal budget. One underexamined facet of marine hydrothermal systems is the role that permeability of the uppermost seafloor veneer plays in the distribution of hydrothermal fluid. As both the initial and final vertical gateway for subsurface fluid circulation, uppermost seafloor permeability may influence the local spatial distribution of hydrothermal flow. A method of deriving a photomosaic from seafloor video was developed and utilized to estimate relative surface permeability in an active hydrothermal area on the Endeavour Segment of the Juan de Fuca Ridge. The mosaic resolves seafloor geology of the axial valley seafloor at submeter resolution over an area greater than 1 km(2). Results indicate that the valley walls and basal talus slope are topographically rugged and unsedimented, providing minimal resistance to fluid transmission. Elsewhere, the axial valley floor is capped by an unbroken blanket of low-permeability sediment, resisting fluid exchange with the subsurface reservoir. Active fluid emission sites were restricted to the high-permeability zone at the base of the western wall. A series of inactive fossil hydrothermal structures form a linear trend along the western bounding wall, oriented orthogonal to the spreading axis. High-temperature vent locations appear to have migrated over 100 m along-ridge-strike over the decade between surveys. While initially an expression of subsurface faulting, this spatial pattern suggests that increases in seafloor permeability from sedimentation may be at least a secondary contributing factor in regulating fluid flow across the seafloor interface.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1525-2027},
	doi = {10.1002/ggge.20209},
	author = {Hearn, Casey K and Homola, Kira L and Johnson, H Paul}
}
@article {Kinsey2013,
	title = {{Sustained volcanically-hosted venting at ultraslow ridges: Piccard Hydrothermal Field, Mid-Cayman Rise}},
	journal = {Earth and Planetary Science Letters},
	volume = {380},
	year = {2013},
	pages = {162{\textendash}168},
	abstract = {At slow spreading mid-ocean ridges sustained submarine venting and the deposition of large seafloor massive sulfide deposits have previously been ascribed to tectonically-controlled hydrothermal circulation unrelated to young volcanic activity. Here, by contrast, we show that the Piccard Hydrothermal Field (PHF), on the ultraslow spreading Mid-Cayman Rise, represents a site of sustained fluid flow and sulfide formation hosted in a neovolcanic setting. The lateral extent and apparent longevity associated with the PHF are comparable to some of the largest tectonically-hosted vent sites known along the slow-spreading Mid-Atlantic Ridge. If such systems recur along all ultraslow ridges, which comprise \~{}20{\%} of the \~{}55,000 km global ridge crest, potential implications would include (i) a higher probability of locating large, economically valuable, mineral deposits along ultraslow ridges together with (ii) larger fluxes than previously anticipated of chemicals released from high-temperature venting entering the oceans along the Atlantic{\textendash}Indian Ocean sectors of the deep-ocean thermohaline conveyor.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0012-821X},
	doi = {10.1016/j.epsl.2013.08.001},
	url = {http://www.sciencedirect.com/science/article/pii/S0012821X13004214},
	author = {Kinsey, J C and German, C R}
}
@article {Bennett2013,
	title = {{Trophic regions of a hydrothermal plume dispersing away from an ultramafic-hosted vent-system: Von Damm vent-site, Mid-Cayman Rise}},
	journal = {Geochemistry, Geophysics, Geosystems},
	volume = {14},
	number = {2},
	year = {2013},
	pages = {317{\textendash}327},
	abstract = {Deep-sea ultramafic-hosted vent systems have the potential to provide large amounts of metabolic energy to both autotrophic and heterotrophic microorganisms in their dispersing hydrothermal plumes. Such vent-systems release large quantities of hydrogen and methane to the water column, both of which can be exploited by autotrophic microorganisms. Carbon cycling in these hydrothermal plumes may, therefore, have an important influence on open-ocean biogeochemistry. In this study, we investigated an ultramafic-hosted system on the Mid-Cayman Rise, emitting metal-poor and hydrogen sulfide-, methane-, and hydrogen-rich hydrothermal fluids. Total organic carbon concentrations in the plume ranged between 42.1 and 51.1 $μ$M (background = 43.2 {\textpm} 0.7 $μ$M (n = 5)) and near-field plume samples with elevated methane concentrations imply the presence of chemoautotrophic primary production and in particular methanotrophy. In parts of the plume characterized by persistent potential temperature anomalies but lacking elevated methane concentrations, we found elevated organic carbon concentrations of up to 51.1 $μ$M, most likely resulting from the presence of heterotrophic communities, their extracellular products and vent larvae. Elevated carbon concentrations up to 47.4 $μ$M were detected even in far-field plume samples. Within the Von Damm hydrothermal plume, we have used our data to hypothesize a microbial food web in which chemoautotrophy supports a heterotrophic community of microorganisms. Such an active microbial food web would provide a source of labile organic carbon to the deep ocean that should be considered in any future studies evaluating sources and sinks of carbon from hydrothermal venting to the deep ocean.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1525-2027},
	doi = {10.1002/ggge.20063},
	url = {http://dx.doi.org/10.1002/ggge.20063},
	author = {Bennett, S A and Coleman, Max and Huber, J A and Reddington, E and Kinsey, J C and McIntyre, C and Seewald, J S and German, C R}
}
@article {Cowen2012,
	title = {{Advanced instrument system for real-time and time-series microbial geochemical sampling of the deep (basaltic) crustal biosphere}},
	journal = {Deep-Sea Research. Part I: Oceanographic Research Papers},
	volume = {61},
	year = {2012},
	pages = {43{\textendash}56},
	abstract = {Integrated Ocean Drilling Program borehole CORK (Circulation Obviation Retrofit Kit) observatories provide long-term access to hydrothermal fluids circulating within the basaltic crust (basement), providing invaluable opportunities to study the deep biosphere. We describe the design and application parameters of the GeoMICROBE instrumented sled, an autonomous sensor and fluid sampling system. The GeoMICROBE system couples with CORK fluid delivery lines to draw large volumes of fluids from crustal aquifers to the seafloor. These fluids pass a series of in-line sensors and an in situ filtration and collection system. GeoMICROBE{\textquoteright}s major components include a primary valve manifold system, a positive displacement primary pump, sensors (e.g., fluid flow rate, temperature, dissolved O2, electrochemistry-voltammetry analyzer), a 48-port in situ filtration and fluid collection system, computerized controller, seven 24~V{\textendash}40~A batteries and wet-mateable (ODI) communications with submersibles. This constantly evolving system has been successfully connected to IODP Hole 1301A on the eastern flank of the Juan de Fuca Ridge. Also described here is a mobile pumping system (MPS), which possesses many of the same components as the GeoMICROBE (e.g., pump, sensors, controller), but is directly powered and controlled in real time via submersible operations; the MPS has been employed repeatedly to collect pristine basement fluids for a variety of geochemical and microbial studies.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0637},
	doi = {10.1016/j.dsr.2011.11.004},
	url = {http://dx.doi.org/10.1016/j.dsr.2011.11.004},
	author = {Cowen, J P and Copson, D A and Jolly, J and Hsieh, C -C and Lin, H -T and Glazer, B T and Wheat, C G}
}
@article {Luther2012,
	title = {{Chemistry, Temperature, and Faunal Distributions at Diffuse-Flow Hydrothermal Vents Comparison of Two Geologically Distinct Ridge Systems}},
	journal = {Oceanography},
	volume = {25},
	number = {1},
	year = {2012},
	month = {mar},
	pages = {234{\textendash}245},
	abstract = {Diffuse-flow, low-temperature areas near hydrothermal vents support life via chemosynthesis: hydrogen sulfide (and other reduced chemical compounds) emanating from the subsurface is oxidized with bottom-water oxygen through bacterial mediation to fix carbon dioxide and produce biomass. This article reviews the in~situ diffuse-flow chemistry (mainly H2S and O2) and temperature data collected in 2006 and 2009 along the Eastern Lau Spreading Center (ELSC), and from 2004 to 2008 at 9{\textdegree}N along the East Pacific Rise (9 N EPR), predominantly around macrofauna that contain endosymbionts at these two hydrothermal vent regions. More than 48,000 and 20,000 distinct chemical and temperature data points were collected with a multi-analyte electrochemical analyzer in the diffuse-flow waters at 9 N EPR and the ELSC, respectively. Despite their different geological settings and different macrofauna (two different species of snails and mussels at the ELSC versus two different species of tubeworms and mussels at 9 N EPR), there are similarities in the temperature and chemistry data, as well as in the distributions of organisms. The pattern of water chemistry preferred by the provannid snails (Alviniconcha~spp., Ifremeria nautilei) and Bathymodiolus brevior at the ELSC is similar to the water chemistry pattern found for the siboglinid tubeworms (Tevnia jerichonana, Riftia pachyptila) and the Bathymodiolus thermophilus mussels at 9 N EPR. The eruptions at 9 N EPR in 2005 and 2006 resulted in increased H2S concentrations, increased H2S/T ratios, and an initial change in the dominant tubeworm species from Riftia pachyptila to Tevnia jerichonana after the eruption created new vent habitats. In 2005, two sites at 9 N EPR showed major increases in the H2S/T ratio from 2004, which suggested a probable eruption in this basalt-dominated system. At the ELSC, there was a decrease in the H2S/T ratio from northern to southern sites, which reflects the change in geological setting from basalt to andesite and the shallower water depths at the southern sites.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {1042-8275},
	doi = {10.5670/oceanog.2012.22},
	author = {Luther, G W and Gartman, A and Yuecel, M and Madison, A S and Moore, T S and Nees, H A and Nuzzio, D B and Sen, A and Lutz, R A and Shank, T M and Fisher, C R}
}
@article {Soule2012,
	title = {{CO2 variability in mid-ocean ridge basalts from syn-emplacement degassing: Constraints on eruption dynamics}},
	journal = {Earth and Planetary Science Letters},
	volume = {327{\textendash}328},
	year = {2012},
	pages = {39{\textendash}49},
	abstract = {Basaltic glasses produced during mid-ocean ridge (MOR) eruptions display a wide range of dissolved CO2 concentrations with respect to equilibrium saturation at their eruption depth. This variability is thought to reflect the dynamics of magma ascent and emplacement, with rapid ascent and depressurization leading to supersaturated conditions and slower ascent resulting in equilibrium saturation. In this study we examine a suite of samples from the 2005{\textendash}06 eruption of the East Pacific Rise (EPR), collected at {\~{}}~200~m intervals along two well-defined flow pathways within a single seafloor eruption to examine syn-emplacement degassing, using measurements of vesicularity, crystallinity, volatile contents, and helium. This new data set is unique because flow pathways of individual MOR lava flows have rarely been mapped and have never been systematically sampled. Here we show that a large range in dissolved CO2 concentrations exists within this single eruption that nearly spans that observed in tens of flows measured along this portion of the EPR crest. This lava flow experienced equilibrium degassing of dissolved CO2 from supersaturated conditions at the vent (and persisting over the first 750~m of flow) toward equilibrium with seafloor pressures along a {\~{}}~2.5~km-long flow path. This was accompanied by an increase in vesicularity and characteristic bubble radius along the flow, indicating the importance of bubble growth by diffusion for degassing. Nearly constant total helium concentrations (melt~+~vesicles) indicate that no bubbles were lost during emplacement. With a model of diffusion-controlled bubble growth constrained by observations from the samples along the largest flow lobe of the eruption, we calculate minimum ascent rates of 0.15~m/s, and {\textendash} for this portion of the eruption {\textendash} an eruption duration of {\~{}}~30~h and flow rates from 0.02 to 0.12~m/s. This study represents the first quantitative assessment of eruption dynamics using volatiles in a mid-ocean ridge lava flow.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0012-821X},
	doi = {10.1016/j.epsl.2012.01.034},
	url = {http://dx.doi.org/10.1016/j.epsl.2012.01.034},
	author = {Soule, S A and Nakata, D S and Fornari, D J and Fundis, A T and Perfit, M R and Kurz, M D}
}
@article {ISI:000311041900011,
	title = {{Community study of tubeworm-associated epizooic meiobenthos from deep-sea cold seeps and hot vents}},
	journal = {MARINE ECOLOGY PROGRESS SERIES},
	volume = {468},
	year = {2012},
	pages = {135{\textendash}148},
	type = {Article},
	abstract = {The permanent metazoan meiofauna associated with vestimentiferan tubeworm aggregations from hydrocarbon seeps of the upper Louisiana slope in the Green Canyon (similar to 550 m) and the lower slope in Atwater Valley (similar to 2200 m) of the Gulf of Mexico was characterized. Meiofauna abundance, diversity, and community composition at genus level were compared between these seep sites, and with those of tubeworms from hydrothermal vents of the East Pacific Rise (Gollner et al. 2007; Mar Ecol Prog Ser 337: 39-49). The abundance was not significantly different between the 2 seep sites, and was also similar to those found at vents. A total of 150 meiobenthic genera were identified from the cold seep sites. While no significant difference in univariate measurements of diversity was detected, a shift in community composition between the shallow and the deep seep site was found. The hot vent communities included a total of only 17 genera and the diversity measurements were significantly lower at vents than at seeps. Also, Bray-Curtis dissimilarity was 97{\%} between the meiobenthic communities from seeps and vents. The genera richness was negatively correlated with maximum temperature and maximum sulfide concentration, and positively correlated with minimum pH value. We conclude that the harsh conditions tubeworms experience at vents compared to the moderate conditions at cold seeps, as well as the longevity of cold seeps surrounded by sedimented deep-sea plains but short-lived vents on basaltic mid-ocean ridges, might explain the contrasting diversity patterns.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0171-8630},
	doi = {10.3354/meps09889},
	author = {Degen, Renate and Riavitz, Laura and Gollner, Sabine and Vanreusel, Ann and Plum, Christoph and Bright, Monika}
}
@article {ISI:000208863600101,
	title = {{Distribution, abundance, and diversity patterns of the thermoacidophilic {\textquoteleft}{\textquoteleft}deep-sea hydrothermal vent euryarchaeota 2{\{}{\textquoteright}{\textquoteright}{\}}}},
	journal = {FRONTIERS IN MICROBIOLOGY},
	volume = {3},
	year = {2012},
	type = {Article},
	abstract = {Cultivation-independent studies have shown that taxa belonging to the {\textquoteleft}{\textquoteleft}deep-sea hydrothermal vent euryarchaeota 2{\{}{\textquoteright}{\textquoteright}{\}} (DHVE2) lineage are widespread at deep-sea hydrothermal vents. While this lineage appears to be a common and important member of the microbial community at vent environments, relatively little is known about their overall distribution and phylogenetic diversity. In this study, we examined the distribution, relative abundance, co-occurrence patterns, and phylogenetic diversity of cultivable thermoacidophilic DHVE2 in deposits from globally distributed vent fields. Results of quantitative polymerase chain reaction assays with primers specific for the DHVE2 and Archaea demonstrate the ubiquity of the DHVE2 at deep-sea vents and suggest that they are significant members of the archaeal communities of established vent deposit communities. Local similarity analysis of pyrosequencing data revealed that the distribution of the DHVE2 was positively correlated with 10 other Euryarchaeota phylotypes and negatively correlated with mostly Crenarchaeota phylotypes. Targeted cultivation efforts resulted in the isolation of 12 axenic strains from six different vent fields, expanding the cultivable diversity of this lineage to vents along the East Pacific Rise and Mid-Atlantic Ridge. Eleven of these isolates shared greater than 97{\%} 16S rRNA gene sequence similarity with one another and the only described isolate of the DHVE2, Aciduliprofundum boonei T469(T). Sequencing and phylogenetic analysis of five protein-coding loci, atpA, EF-2, radA, rpoB, and secY, revealed clustering of isolates according to geographic region of isolation. Overall, this study increases our understanding of the distribution, abundance, and phylogenetic diversity of the DHVE2.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {1664-302X},
	doi = {10.3389/fmicb.2012.00047},
	author = {Flores, Gilberto E and Wagner, Isaac D and Liu, Yitai and Reysenbach, Anna-Louise}
}
@article {Fornari2012,
	title = {{The East Pacific Rise Between 9 degrees N and 10 degrees N: Twenty-Five Years of Integrated, Multidisciplinary Oceanic Spreading Center Studies}},
	journal = {Oceanography},
	volume = {25},
	number = {1},
	year = {2012},
	month = {mar},
	pages = {18{\textendash}43},
	publisher = {OCEANOGRAPHY SOC},
	address = {ROCKVILLE; P.O. BOX 1931, ROCKVILLE, MD USA},
	abstract = {The East Pacific Rise from {\~{}}~9{\textendash}10{\textdegree}N is an archetype for a fast-spreading mid-ocean ridge. In particular, the segment near 9{\textdegree}50{\textquoteright}N has been the focus of multidisciplinary research for over two decades, making it one of the best-studied areas of the global ridge system. It is also one of only two sites along the global ridge where two historical volcanic eruptions have been observed. This volcanically active segment has thus offered unparalleled opportunities to investigate a range of complex interactions among magmatic, volcanic, hydrothermal, and biological processes associated with crustal accretion over a full magmatic cycle. At this 9{\textdegree}50{\textquoteright}N site, comprehensive physical oceanographic measurements and modeling have also shed light on linkages between hydrodynamic transport of larvae and other materials and biological dynamics influenced by magmatic processes. Integrated results of high-resolution mapping, and both in~situ and laboratory-based geophysical, oceanographic, geochemical, and biological observations and sampling, reveal how magmatic events perturb the hydrothermal system and the biological communities it hosts.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {1042-8275},
	doi = {10.5670/oceanog.2012.02},
	author = {Fornari, D J and Von Damm, K L and Bryce, Julia G and Cowen, J P and Ferrini, Vicki and Fundis, A and Lilley, M D and Luther, G W and Mullineaux, L S and Perfit, M R and Meana-Prado, M F and Rubin, K H and Seyfried, W E and Shank, T M and Soule, S A and Tolstoy, M and White, S M}
}
@article {Beinart2012,
	title = {{Evidence for the role of endosymbionts in regional-scale habitat partitioning by hydrothermal vent symbioses}},
	journal = {Proceedings of the National Academy of Sciences of the United States of America},
	volume = {109},
	number = {47},
	year = {2012},
	month = {nov},
	pages = {E3241{\textendash}E3250},
	abstract = {Deep-sea hydrothermal vents are populated by dense communities of animals that form symbiotic associations with chemolithoautotrophic bacteria. To date, our understanding of which factors govern the distribution of host/symbiont associations (or holobionts) in nature is limited, although host physiology often is invoked. In general, the role that symbionts play in habitat utilization by vent holobionts has not been thoroughly addressed. Here we present evidence for symbiont-influenced, regional-scale niche partitioning among symbiotic gastropods (genus Alviniconcha) in the Lau Basin. We extensively surveyed Alviniconcha holobionts from four vent fields using quantitative molecular approaches, coupled to characterization of high-temperature and diffuse vent-fluid composition using gastight samplers and in situ electrochemical analyses, respectively. Phylogenetic analyses exposed cryptic host and symbiont diversity, revealing three distinct host types and three different symbiont phylotypes (one $ε$-proteobacteria and two $\gamma$-proteobacteria) that formed specific associations with one another. Strikingly, we observed that holobionts with $ε$-proteobacterial symbionts were dominant at the northern fields, whereas holobionts with $\gamma$-proteobacterial symbionts were dominant in the southern fields. This pattern of distribution corresponds to differences in the vent geochemistry that result from deep subsurface geological and geothermal processes. We posit that the symbionts, likely through differences in chemolithoautotrophic metabolism, influence niche utilization among these holobionts. The data presented here represent evidence linking symbiont type to habitat partitioning among the chemosynthetic symbioses at hydrothermal vents and illustrate the coupling between subsurface geothermal processes and niche availability.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0027-8424},
	doi = {10.1073/pnas.1202690109},
	author = {Beinart, R A and Sanders, J G and Faure, B and Sylva, S P and Lee, R W and Becker, E L and Gartman, A and Luther, G W and Seewald, J S and Fisher, C R and Girguis, P R}
}
@article {ISI:000311470800005,
	title = {{Geographic expansion of hermatypic and ahermatypic corals in the Gulf of Mexico, and implications for dispersal and recruitment}},
	journal = {JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY},
	volume = {436},
	year = {2012},
	month = {dec},
	pages = {36{\textendash}49},
	type = {Article},
	abstract = {The {\textgreater}3000 oil/gas platforms in the northern Gulf of Mexico (GOM) provide shallow hard-substratum in a soft-bottom dominated ecosystem. Excepting the Flower Garden Banks (FGB) and deeper mesophotic banks, no shallow hard substrate has been available offshore since the Holocene. Platforms have facilitated coral geographic expansion in this region. We determined the distribution, abundance, and species diversity patterns on 42 platforms in this region, at {\textless}= 37 m depth, along four cross-shelf transects: 1) S-SE from Corpus Christi, Texas; 2) S from Lake Sabine, Texas; 3) S from Terrebonne Bay, Louisiana; and 4) S-SW from Mobile, Alabama. Nine hermatypic, two ahermatypic, and one hydrozoan coral were found: hermatypes-Madracis decactis. Diploria strigosa, Montastraea cavernosa, Porites astreoides, Madracis formosa, Colpophyllia natans, Stephanocoenia intercepta, Stephanocoenia michelinii, and Millepora alcicornis (Hydrozoa); ahermatypes-Tubastraea cocci flea. Oculina diffusa, and Phyllangia americana. Pattern-seeking analyses identified four community types: one with no hermatypic corals (inner and mid-shelf); one high diversity set of platforms, dominated by abundant Madracis decactis, occurring with four other species per platform (outer shelf); and a third dominated by Madracis decactis, in low abundance, co-dominated by D. strigosa and Millepora alcicornis (outer shelf). Species diversity was highest around the Flower Garden Banks. Hermatypic density and D. strigosa (broadcaster) in particular, was maximum around the FGB, extending northwards. Madracis decactis (brooder) densities peaked at the shelf edge, off Terrebonne Bay, Louisiana. Montastraea cavernosa densities were bimodal, peaking near the FGB and east of the Mississippi River. The FGB appear to be the source of hermatypic corals for most platform populations. Differences between the distribution patterns of Madracis decactis and D. strigosa, Montastraea cavernosa, and O. diffusa suggest that the brooder effects longer recruitment distances by averaging larval dispersal over a broad range of hydrographic conditions throughout the year, while the broadcaster utilizes only one set of conditions over the same period. Averaging respective dispersal strategies over decades may have produced the observed disparate distribution patterns. Ahermatypic coral density exceeded hermatypic density by 10,000 fold, peaking south of Terrebonne Bay, Louisiana and Mobile, Alabama, due to T. coccinea. Ahermatypic diversity was highest off Matagorda Island, Texas, decreasing from west to east. Multi-variate pattern-seeking analysis (PATN) identified four ahermatypic community types: one dominated by O. diffusa and T. coccinea in low abundances (shelf-wide distribution); one characterized by the absence of ahermatypic corals (generally near-shore); one heavily dominated by T. coccinea, and also by O. diffusa in low abundances (mid-shelf to shelf edge); and one dominated by T. coccinea, co-dominated by both P. americana and O. diffusa in low abundances (shelf-edge). T. coccinea and P. americana appear to be derived from the southern GOM off Mexico or the Caribbean. Unlike shrinking coral populations in other parts of the world, corals have expanded their range substantially in the northern Gulf of Mexico, in areas previously devoid or near devoid of corals, facilitated by thousands of oil/gas platforms deployed for decades throughout the region. It is also possible that coral populations on these platforms may act as potential larval sources for the FGB in the event of a mass coral mortality there. (C) 2012 Elsevier B.V. All rights reserved.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0022-0981},
	doi = {10.1016/j.jembe.2012.08.009},
	author = {Sammarco, Paul W and Atchison, Amy D and Boland, Gregory S and Sinclair, James and Lirette, Angela}
}
@article {ISI:000306502100006,
	title = {{Hippea jasoniae sp nov and Hippea alviniae sp nov., thermoacidophilic members of the class Deltaproteobacteria isolated from deep-sea hydrothermal vent deposits}},
	journal = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
	volume = {62},
	number = {6},
	year = {2012},
	month = {jun},
	pages = {1252{\textendash}1258},
	type = {Article},
	abstract = {Thirteen novel, obligately anaerobic, thermoacidophilic bacteria were isolated from deep-sea hydrothermal vent sites. Four of the strains, designated EP5-r(T), KM1, Mar08-272r(T) and Mar08-368r, were selected for metabolic and physiological characterization. With the exception of strain EP5-r(T), all strains were short rods that grew between 40 and 72 degrees C, with optimal growth at 60-65 degrees C. Strain EP5-r(T) was more ovoid in shape and grew between 45 and 75 degrees C, with optimum growth at 60 degrees C. The pH range for growth of all the isolates was between pH 3.5 and 5.5 (optimum pH 4.5 to 5.0). Strain Mar08-272r(T) could only grow up to pH 5.0. Elemental sulfur was required for heterotrophic growth on acetate, succinate, Casamino acids and yeast extract. Strains EP5-r(T), Mar08-272r(T) and Mar08-368r could also use fumarate, while strains EP5-r(T), KM1 and Mar08-272r(T) could also use propionate. All isolates were able to grow chemolithotrophically on H-2, CO2, sulfur and vitamins. Phylogenetic analysis of 16S rRNA gene sequences placed all isolates within the family Desulfurellaceae of the class Deltaproteobacteria, with the closest cultured relative being Hippea maritima MH2T (similar to 95-98{\%} gene sequence similarity). Phylogenetic analysis also identified several isolates with at least one intervening sequence within the 16S rRNA gene. The genomic DNA G+C contents of strains EP5-r(T), KM1, Mar08-272r(T) and Mar08-368r were 37.1, 42.0, 35.6 and 37.9 mol{\%}, respectively. The new isolates differed most significantly from H. maritima MH2T in their phylogenetic placement and in that they were obligate thermoacidophiles. Based on these phylogenetic and phenotypic properties, the following two novel species are proposed: Hippea jasoniae sp. nov. (type strain Mar08-272r(T)=DSM 24585(T)=OCM 985(T)) and Hippea alviniae sp. nov. (type strain EP5-r(T)=DSM 24586(T)=OCM 986(T)).},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {1466-5026},
	doi = {10.1099/ijs.0.033001-0},
	author = {Flores, Gilberto E and Hunter, Ryan C and Liu, Yitai and Mets, Anchelique and Schouten, Stefan and Reysenbach, Anna-Louise}
}
@article {Kim2012,
	title = {{Hydrothermal vent community zonation along environmental gradients at the Lau back-arc spreading center}},
	journal = {Deep-Sea Research. Part I: Oceanographic Research Papers},
	volume = {62},
	year = {2012},
	pages = {10{\textendash}19},
	abstract = {The Lau back-arc spreading center exhibits gradients in hydrothermal vent habitat characteristics from north to south. Biological zonation within a few meters of vents has been described as temperature driven. We constructed georeferenced photomosaics of the seafloor out to tens of meters beyond vents to describe peripheral zonation and explore correlations between environmental conditions and the biological community. Cluster analysis separated northern sites from southern sites, corresponding to a break in substrate from basalt in the north to andesite in the south. Northern sites were dominated by anemones, and southern by sponges. A previous suggestion that dominants may be dependent on friability of the substrate was not supported; when visually distinguishable, individual species within taxa showed different patterns. Northern sites hosted proportionally more suspension feeding species. Sulfide that can support microbial food sources is at higher concentrations at these sites, though bathymetry that may enhance bottom currents is less rugged. Northern sites had higher diversity that may result from the overall northwards flow, which would generally permit easier dispersal downcurrent, though we observed no difference in dispersal strategies at different sites.},
	keywords = {ROV Jason (Remotely Operated Vehicle), ROV Medea (Remotely Operated Vehicle)},
	issn = {0967-0637},
	doi = {10.1016/j.dsr.2011.12.010},
	url = {http://dx.doi.org/10.1016/j.dsr.2011.12.010},
	author = {Kim, S and Hammerstrom, K}
}
@article {White2012,
	title = {{Impact of the Deepwater Horizon oil spill on a deep-water coral community in the Gulf of Mexico}},
	journal = {Proceedings of the National Academy of Sciences of the United States of America},
	volume = {109},
	number = {50},
	year = {2012},
	month = {dec},
	pages = {20303{\textendash}20308},
	abstract = {To assess the potential impact of the Deepwater Horizon oil spill on offshore ecosystems, 11 sites hosting deep-water coral communities were examined 3 to 4 mo after the well was capped. Healthy coral communities were observed at all sites {\textgreater}20 km from the Macondo well, including seven sites previously visited in September 2009, where the corals and communities appeared unchanged. However, at one site 11 km southwest of the Macondo well, coral colonies presented widespread signs of stress, including varying degrees of tissue loss, sclerite enlargement, excess mucous production, bleached commensal ophiuroids, and covering by brown flocculent material (floc). On the basis of these criteria the level of impact to individual colonies was ranked from 0 (least impact) to 4 (greatest impact). Of the 43 corals imaged at that site, 46{\%} exhibited evidence of impact on more than half of the colony, whereas nearly a quarter of all of the corals showed impact to {\textgreater}90{\%} of the colony. Additionally, 53{\%} of these corals{\textquoteright} ophiuroid associates displayed abnormal color and/or attachment posture. Analysis of hopanoid petroleum biomarkers isolated from the floc provides strong evidence that this material contained oil from the Macondo well. The presence of recently damaged and deceased corals beneath the path of a previously documented plume emanating from the Macondo well provides compelling evidence that the oil impacted deep-water ecosystems. Our findings underscore the unprecedented nature of the spill in terms of its magnitude, release at depth, and impact to deep-water ecosystems.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0027-8424},
	doi = {10.1073/pnas.1118029109},
	author = {White, H K and Hsing, P-Y and Cho, W and Shank, T M and Cordes, E E and Quattrini, A M and Nelson, R K and Camilli, R and Demopoulos, A W J and German, C R and Brooks, J M and Roberts, H H and Shedd, W and Reddy, C M and Fisher, C R}
}
@article {Lin2012,
	title = {{Inorganic chemistry, gas compositions and dissolved organic carbon in fluids from sedimented young basaltic crust on the Juan de Fuca Ridge flanks}},
	journal = {Geochimica et Cosmochimica Acta},
	volume = {85},
	year = {2012},
	pages = {213{\textendash}227},
	abstract = {The permeable upper oceanic basement serves as a plausible habitat for a variety of microbial communities. There is growing evidence suggesting a substantial subseafloor biosphere. Here new time series data are presented on key inorganic species, methane, hydrogen and dissolved organic carbon (DOC) in ridge flank fluids obtained from subseafloor observatory CORKs (Circulation Obviation Retrofit Kits) at Integrated Ocean Drilling Program (IODP) boreholes 1301A and 1026B. These data show that the new sampling methods (Cowen et al., 2012) employed at 1301A result in lower contamination than earlier studies. Furthermore, sample collection methods permitted most chemical analyses to be performed from aliquots of single large volume samples, thereby allowing more direct comparison of the data. The low phosphate concentrations (0.06{\textendash}0.2~$μ$M) suggest that relative to carbon and nitrogen, phosphorus could be a limiting nutrient in the basement biosphere. Coexisting sulfate (17{\textendash}18~mM), hydrogen sulfide (\~{}0.1~$μ$M), hydrogen (0.3{\textendash}0.7~$μ$M) and methane (1.5{\textendash}2~$μ$M) indicates that the basement aquifer at 1301A either draws fluids from multiple flow paths with different redox histories or is a complex environment that is not thermodynamically controlled and may allow co-occurring metabolic pathways including sulfate reduction and methanogenesis. The low DOC concentrations (11{\textendash}18~$μ$M) confirm that ridge flank basement is a net DOC sink and ultimately a net carbon sink. Based on the net amounts of DOC, oxygen, nitrate and sulfate removed (\~{}30~$μ$M, \~{}80~$μ$M, \~{}40~$μ$M and \~{}10~mM, respectively) from entrained bottom seawater, organic carbon may be aerobically or anaerobically oxidized in biotic and/or abiotic processes.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0016-7037},
	doi = {10.1016/j.gca.2012.02.017},
	url = {http://dx.doi.org/10.1016/j.gca.2012.02.017},
	author = {Lin, H -T and Cowen, J P and Olson, E J and Amend, J P and Lilley, M D}
}
@article {Tivey2012,
	title = {{Links from Mantle to Microbe at the Lau Integrated Study Site:Insights from a Back-Arc Spreading Center}},
	journal = {Oceanography},
	volume = {25},
	number = {1},
	year = {2012},
	month = {mar},
	pages = {62{\textendash}77},
	abstract = {The Lau Integrated Study Site (ISS) has provided unique opportunities for study of ridge processes because of its back-arc setting in the southwestern Pacific. Its location allows study of a biogeographical province distinct from those of eastern Pacific and mid-Atlantic ridges, and crustal compositions along the ridge lie outside the range of mid-ocean ridge crustal compositions. The Lau ISS is located above a subduction zone, at an oblique angle. The underlying mantle receives water and other elements derived from the downgoing lithospheric slab, with an increase in slab influence from north to south. Water lowers the mantle melting temperature and leads to greater melt production where the water flux is greater, and to distinctive regional-scale gradients along the ridge. There are deeper faulted axial valleys with basaltic volcanism in the north and inflated axial highs with andesites in the south. Differences in igneous rock composition and release of magmatic volatiles affect compositions of vent fluids and deposits. Differences in vent fluid compositions and small-scale diffuse-flow regimes correlate with regional-scale patterns in microbial and megafaunal distributions. The interdisciplinary research effort at the Lau ISS has successfully identified linkages between subsurface processes and deep-sea biological communities, from mantle to microbe to megafauna.},
	keywords = {AUV ABE (Autonomous Benthic Explorer), ROV Jason (Remotely Operated Vehicle)},
	issn = {1042-8275},
	doi = {10.5670/oceanog.2012.04},
	author = {Tivey, M K and Becker, E and Beinart, R and Fisher, C R and Girguis, P R and Langmuir, C H and Michael, P J and Reysenbach, A -L}
}
@article {ISI:000301095200020,
	title = {{Low-Temperature Hydrothermal Plumes in the Near-Bottom Boundary Layer at Endeavour Segment, Juan de Fuca Ridge}},
	journal = {OCEANOGRAPHY},
	volume = {25},
	number = {1, SI},
	year = {2012},
	month = {mar},
	pages = {192{\textendash}195},
	type = {Article},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1042-8275},
	author = {Hautala, Susan and Johnson, H Paul and Pruis, Matthew and Garcia-Berdeal, Irene and Bjorklund, Tor}
}
@article {Caress2012,
	title = {{Repeat bathymetric surveys at 1-metre resolution of lava flows erupted at Axial Seamount in April 2011}},
	journal = {Nature Geoscience},
	volume = {5},
	number = {7},
	year = {2012},
	month = {jul},
	pages = {483{\textendash}488},
	publisher = {NATURE PUBLISHING GROUP},
	address = {NEW YORK; 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA},
	abstract = {At sites with frequent submarine volcanic activity, it is difficult to discern between new and pre-existing lava flows. In particular, the distribution of the fissures from which lava erupts, the routes taken by lava flows and the relationship between the new flows and the pre-existing seafloor bathymetry are often unclear. The volcanic and hydrothermal systems of Axial Seamount submarine volcano in the Pacific Ocean have been studied intensively since eruptions were detected in 1998 (refs~1, 2) and 2011 (ref.~3). Here we combine pre- and post-eruption bathymetric surveys3, 4, 5, 6, 7, 8, with 1-m lateral resolution and 0.2-m vertical precision, to precisely map the extent and thickness of the lava flows, calculate the volume of lava and unambiguously identify eruptive fissures from the April 2011 eruption. Where the new lava flows extend beyond the boundaries of the repeated surveys, we use shipboard multibeam surveys to map the flows with lower resolution. We show that the eruption produced both sheet and lobate flows associated with high eruption rates and low-eruption-rate pillow mounds. We find that lava flows erupted from new as well as existing fissures and tended to reoccupy existing flow channels. This reoccupation makes it difficult to map submarine flows produced during one eruption without before-and-after bathymetric surveys.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1752-0894},
	doi = {10.1038/ngeo1496},
	author = {Caress, D W and Clague, D A and Paduan, J B and Martin, J F and Dreyer, B M and Chadwick, W W and Denny, Alden and Kelley, D S}
}
@article {McCulloch2012,
	title = {{Resilience of Cold-Water Scleractinian Corals to Ocean Acidification: Boron Isotopic Systematics of pH and Saturation State Up-Regulation}},
	journal = {Geochimica et Cosmochimica Acta},
	volume = {87},
	year = {2012},
	pages = {21{\textendash}34},
	abstract = {The boron isotope systematics has been determined for azooxanthellate scleractinian corals from a wide range of both deep-sea and shallow-water environments. The aragonitic coral species, Caryophyllia smithii, Desmophyllum dianthus, Enallopsammia rostrata, Lophelia pertusa, and Madrepora oculata, are all found to have relatively high $δ$11B compositions ranging from 23.2{\textperthousand} to 28.7{\textperthousand}. These values lie substantially above the pH-dependent inorganic seawater borate equilibrium curve, indicative of strong up-regulation of pH of the internal calcifying fluid (pHcf), being elevated by \~{}0.6{\textendash}0.8~units ($Δ$pH) relative to ambient seawater. In contrast, the deep-sea calcitic coral Corallium sp. has a significantly lower $δ$11B composition of 15.5{\textperthousand}, with a corresponding lower $Δ$pH value of \~{}0.3~units, reflecting the importance of mineralogical control on biological pH up-regulation. The solitary coral D. dianthus was sampled over a wide range of seawater pHT and shows an approximate linear correlation with $Δ$pHDesmo~=~6.43~-~0.71pHT (r2~=~0.79). An improved correlation is however found with the closely related parameter of seawater aragonite saturation state, where $Δ$pHDesmo~=~1.09~-~0.14${\O}mega$arag (r2~=~0.95), indicating the important control that carbonate saturation state has on calcification. The ability to up-regulate internal pHcf, and consequently ${\O}mega$cf, of the calcifying fluid is therefore a process present in both azooxanthellate and zooxanthellate aragonitic corals, and is attributed to the action of Ca2+-ATPase in modulating the proton gradient between seawater and the site of calcification. These findings also show that the boron isotopic compositions ($δ$11Bcarb) of aragonitic corals are highly systematic and consistent with direct uptake of the borate species within the biologically controlled extracellular calcifying medium. We also show that the relatively strong up-regulation of pH and consequent elevation of the internal carbonate saturation state (${\O}mega$cf \~{}8.5 to \~{}13) at the site of calcification by cold-water corals, facilitates calcification at or in some cases below the aragonite saturation horizon, providing a greater ability to adapt to the already low and now decreasing carbonate ion concentrations. Although providing greater resilience to the effects of ocean acidification and enhancing rates of calcification with increasing temperature, the process of internal pHcf up-regulation has an associated energetic cost, and therefore growth-rate cost, of \~{}10{\%} per 0.1 pH unit decrease in seawater pHT. Furthermore, as the aragonite saturation horizon shoals with rapidly increasing pCO2 and ${\O}mega$arag~~},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0016-7037},
	doi = {10.1016/j.gca.2012.03.027},
	url = {http://dx.doi.org/10.1016/j.gca.2012.03.027},
	author = {McCulloch, M and Trotter, J and Montagna, P and Falter, J and Dunbar, R and Freiwald, A and F{\"o}rsterra, G and Correa, M L and Maier, C and R{\"u}ggeberg, A and Taviani, M}
}
@article {Chadwick2012,
	title = {{Seafloor deformation and forecasts of the April 2011 eruption at Axial Seamount}},
	journal = {Nature Geoscience},
	volume = {5},
	number = {7},
	year = {2012},
	month = {jul},
	pages = {474{\textendash}477},
	publisher = {NATURE PUBLISHING GROUP},
	address = {NEW YORK; 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA},
	abstract = {Axial Seamount is an active submarine volcano located at the intersection between the Cobb hotspot and the Juan de Fuca spreading centre in the northeast Pacific Ocean1, 2. The volcano has been closely monitored since it erupted in 1998 (refs~3, 4). Since then, Axial Seamount seemed to exhibit a similar inflation{\textendash}deflation cycle to basaltic volcanoes on land and, on that basis, was expected to erupt again sometime before 2014 or 2020 (refs~5, 6). In April 2011 Axial Seamount erupted. Here we report continuous measurements of ocean bottom pressure that document the deflation{\textendash}inflation cycle of Axial Seamount between 1998 and 2011. We find that the volcano inflation rate, caused by the intrusion of magma, gradually increased in the months leading up to the 2011 eruption. Sudden uplift occurred 40{\textendash}55 min before the eruption onset, which we interpret as a precursor event. Based on our measurements of ground deformation through the entire eruption cycle at Axial Seamount, we suggest that another eruption could occur as early as 2018. We propose that the long-term eruptive cycle of Axial Seamount could be more predictable compared with its subaerial counterparts because the volcano receives a relatively steady supply of magma through the Cobb hotspot and because it is located on thin oceanic crust at a spreading plate boundary.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {1752-0894},
	doi = {10.1038/ngeo1464},
	author = {Chadwick, W W and Nooner, S L and Butterfield, D A and Lilley, M D}
}
@article {Dziak2012,
	title = {{Seismic precursors and magma ascent before the April 2011 eruption at Axial Seamount}},
	journal = {Nature Geoscience},
	volume = {5},
	number = {7},
	year = {2012},
	month = {jul},
	pages = {478{\textendash}482},
	publisher = {NATURE PUBLISHING GROUP},
	address = {NEW YORK; 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA},
	abstract = {Volcanoes at spreading centres on land often exhibit seismicity and ground inflation months to years before an eruption, caused by a gradual influx of magma to the source reservoir1, 2, 3, 4. Deflation and seismicity can occur on time scales of hours to days, and result from the injection of magma into adjacent rift zones5, 6, 7, 8. Volcanoes at submarine rift zones, such as Axial Seamount in the northeast Pacific Ocean, have exhibited similar behaviour9, 10, 11, 12, but a direct link between seismicity, seafloor deformation and magma intrusion has never been demonstrated. Here we present recordings from ocean-bottom hydrophones and an established array of bottom-pressure recorders that reveal patterns of both microearthquakes and seafloor deformation at Axial Seamount on the Juan de Fuca Ridge, before it erupted in April 2011. Our observations show that the rate of seismicity increased steadily during a period of several years, leading up to an intrusion and eruption of magma that began on 6 April 2011. We also detected a sudden increase in seismo-acoustic energy about 2.6 h before the eruption began. Our data indicate that access to real-time seismic data, projected to be available in the near future, might facilitate short-term forecasting and provide sufficient lead-time to prepare in situ instrumentation before future intrusion and eruption events.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {1752-0894},
	doi = {10.1038/ngeo1490},
	author = {Dziak, R P and Haxel, J H and Bohnenstiehl, D R and Chadwick, W W and Nooner, S L and Fowler, M J and Matsumoto, H and Butterfield, D A}
}
@article {ISI:000307406000002,
	title = {{Squat lobsters (Crustacea: Decapoda: Galatheoidea and Chirostyloidea) collected during the TALUD XIV cruise in the Gulf of California, Mexico, and rediscovery of Gastroptychus perarmatus (Haig, 1968) in the eastern Pacific}},
	journal = {ZOOTAXA},
	number = {3418},
	year = {2012},
	month = {aug},
	pages = {28{\textendash}40},
	type = {Article},
	abstract = {Seven species of squat lobsters were collected during the TALUD XIV cruise in the Gulf of California, Mexico. Gastroptychus perarmatus (Haig, 1968) was collected for the second time since it was described and represents a first record of the genus in the tropical eastern Pacific. Its association with gorgonians is also noted from color pictures taken during a deep-water dive in another cruise in the area. Janetogalathea californiensis (Benedict, 1902) was captured in four sampling stations, in the same area where it has been previously reported. Three species of Munida Leach, 1820 were collected (M. bapensis Hendrickx, 2000, M. mexicana Benedict, 1902, and M. tenella Benedict, 1902). Records of M. bapensis of this cruise combined with additional captures of this species in 2007 in the same area indicate that it is the most abundant deep-water species of squat lobster in the northern part of the central Gulf of California. Among the species of Munida, M. tenella was second in abundance and included specimens much larger than previously known. The single record for M. mexicana fits within the currently known depth and geographical ranges. Only one species of Munidopsis Whiteaves, 1874 (M. depressa Faxon, 1892) was collected, in one of the deeper sampling stations visited during the cruise and its northernmost distribution limit within the Gulf of California is increased by ca two degrees of latitude. The seventh species collected during this survey, Pleuroncodes planipes Stimpson, 1860, is a common inhabitant of the California Current and the Gulf of California.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1175-5326},
	author = {Hendrickx, Michel E}
}
@article {Chadwick2012a,
	title = {{Submarine landslide triggered by volcanic eruption recorded by in situ hydrophone}},
	journal = {Geology},
	volume = {40},
	number = {1},
	year = {2012},
	month = {jan},
	pages = {51{\textendash}54},
	publisher = {GEOLOGICAL SOC AMER, INC},
	address = {BOULDER; PO BOX 9140, BOULDER, CO 80301-9140 USA},
	abstract = {NW Rota-1 is a submarine volcano in the Mariana volcanic arc that is notable as the site where underwater explosive eruptions were first witnessed in A.D. 2004. After years of continuous low-level eruptive activity, a major landslide occurred at NW Rota-1 in August 2009, triggered by an unusually large eruption that produced 10 times the acoustic energy of the background level of activity. An anomalous earthquake swarm preceded the eruption, suggesting that the sequence started with a magmatic intrusion and associated faulting beneath the volcano. We quantify the size and extent of the landslide using bathymetric resurveys and interpret the timing of events using data from an in situ hydrophone. This is the first instrumental documentation of an earthquake-eruption-landslide sequence at a submarine volcano, and illustrates the close interaction between magmatic activity and mass wasting events in the growth of undersea arc volcanoes.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0091-7613},
	doi = {10.1130/G32495.1},
	author = {Chadwick, W W and Dziak, R P and Haxel, J H and Embley, R W and Matsumoto, H}
}
@article {ISI:000299990700001,
	title = {{Subseafloor nitrogen transformations in diffuse hydrothermal vent fluids of the Juan de Fuca Ridge evidenced by the isotopic composition of nitrate and ammonium}},
	journal = {GEOCHEMISTRY GEOPHYSICS GEOSYSTEMS},
	volume = {13},
	year = {2012},
	month = {feb},
	type = {Article},
	abstract = {Little is known about dissolved inorganic nitrogen (DIN) transformations in hydrothermal vent (HV) fluids. Here, we present the first isotopic measurements of nitrate (delta N-15 and delta O-18) and ammonium (delta N-15) from three HV fields on the Juan de Fuca ridge (NE-Pacific). The dominant process that drives DIN concentration variations in low-T diffuse fluids is water mass mixing below the seafloor, with no effect on the DIN isotope ratios. Strong inter-site variations in the concentration and delta N-15 of NH4+ in high-T fluids suggest different subsurface nitrogen (N) sources (deep-sea nitrate versus organic sediments) for hydrothermally discharged ammonium. Low NH4+ community N isotope effects ({\textless}3 parts per thousand) for net NH4+ consumption suggest an important contribution from gross ammonium regeneration in low-T fluids. Elevation of HV nitrate N-15/N-14 and O-18/O-16 over deep-sea mean isotope values at some sites, concomitant with decreased nitrate concentrations, indicate assimilatory or dissimilatory nitrate consumption by bacteria in the subsurface, with relatively low community N isotope effects ((15)epsilon(k) {\textless} 3 parts per thousand). The low N isotope effects suggest that nitrate assimilation or denitrification occur in bacterial mats, and/or in situ production of low delta N-15 nitrate. A significantly stronger relative increase for nitrate delta O-18 than for delta N-15 was observed at many sites, resulting in marked deviations from the 1:1 relationship for nitrate delta N-15 versus delta O-18 that is expected for nitrate reduction in marine settings. Simple box-model calculation show that the observed un-coupling of N and O nitrate isotope ratios is consistent with nitrate regeneration by either nitrite reoxidation and/or partial nitrification of hydrothermal ammonium (possibly originating from N-2 fixation). Our isotope data confirm the role of subsurface microbial communities in modulating hydrothermal fluxes to the deep ocean.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {1525-2027},
	doi = {10.1029/2011GC003863},
	author = {Bourbonnais, Annie and Lehmann, Moritz F and Butterfield, David A and Juniper, S Kim}
}
@article {Pester2012,
	title = {{Subseafloor phase equilibria in high-temperature hydrothermal fluids of the Lucky Strike Seamount (Mid-Atlantic Ridge, 37{\textdegree}17{\textquoteright}N)}},
	journal = {Geochimica et Cosmochimica Acta},
	volume = {90},
	year = {2012},
	pages = {303{\textendash}322},
	abstract = {As part of an integrated study conducted at the Lucky Strike Seamount (Mid-Atlantic Ridge, 37{\textdegree}17'N) in 2008, gas-tight sampling devices were used to collect high-temperature (\~{}300~{\textdegree}C) hydrothermal fluids issuing from sulfide structures distributed throughout the vent field located in the summit depression. Compared with previous observations from 1993 to 1997, the most substantial changes in vent fluid compositions are dramatically increased CO2 concentrations (\~{}5{\texttimes}, up to 133~mmol/L) and the observation of vent fluids enriched in dissolved chloride relative to seawater. Combined with an increase in $δ$13CCO2$δ$13CCO2 values by \~{}4{\textperthousand} in 2008, the elevated CO2 indicates replenishment of the magmatic heat source and may be indicative of a recent magmatic event. The additional supporting fluid chemistry is, however, similar to that of the previous sampling intervals, necessitating a reassessment of the subseafloor controls on vent fluid chemistry at Lucky Strike in the context of recently obtained geophysical data that provides the depth/extent of a steady-state magma chamber. Two-phase behavior is indicated by the chloride variability in the vent fluids; and comparison with experimental data for the associated chloride-dependent partitioning of minor/trace elements suggests the possibility of a similar source fluid for all the vent structures, while limiting the likelihood of shallow phase separation and subseafloor mixing for the hydrothermal end-members. A recently calibrated Fe/Mn geothermometer indicates minimum subseafloor equilibration temperatures of 350{\textendash}385~{\textdegree}C. However, constraints imposed by dissolved Si/Cl in conjunction with geophysical observations are consistent with peak reaction conditions at temperatures of 430{\textendash}475~{\textdegree}C and pressures near the top of the axial magma chamber (\~{}410{\textendash}480~bars), where magmatic CO2 becomes entrained in the circulating fluids. The distance between the magma chamber and the seafloor at Lucky Strike is substantially greater than at most faster spreading ridges; and we propose the resulting increased residence time in the up-flow zone leads to the re-equilibration of temperature sensitive transition metals at conditions less extreme than those associated with peak reaction. Agreement between experimental data, thermodynamic model calculations, and dissolved concentrations of Fe, Cu, Zn, H2, and H2S in the Lucky Strike fluids reinforce the hypothesis of pH-redox equilibria for transition metals at relatively oxidizing conditions and temperatures predicted by the empirical Fe/Mn geothermometer. In-situ pH measurements of the high-temperature fluids exiting the seafloor are also consistent with the model calculations.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1016/j.gca.2012.05.018},
	url = {http://dx.doi.org/10.1016/j.gca.2012.05.018},
	author = {Pester, N J and Reeves, E P and Rough, M E and Ding, K and Seewald, J S and Seyfried, W E}
}
@article {ISI:000312516200003,
	title = {{Thermosulfurimonas dismutans gen. nov., sp nov., an extremely thermophilic sulfur-disproportionating bacterium from a deep-sea hydrothermal vent}},
	journal = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
	volume = {62},
	number = {11},
	year = {2012},
	month = {nov},
	pages = {2565{\textendash}2571},
	type = {Article},
	abstract = {An extremely thermophilic, anaerobic, chemolithoautotrophic bacterium (strain S95(T)) was isolated from a deep-sea hydrothermal vent chimney located on the Eastern Lau Spreading Center, Pacific Ocean, at a depth of 1910 m. Cells of strain S95(T) were oval to short Gram-negative rods, 0.5-0.6 mu m in diameter and 1.0-1.5 mu m in length, growing singly or in pairs. Cells were motile with a single polar flagellum. The temperature range for growth was 50-92 degrees C, with an optimum at 74 degrees C. The pH range for growth was 5.5-8.0, with an optimum at pH 7.0. Growth of strain S95(T) was observed at NaCl concentrations ranging from 1.5 to 3.50/o (w/v). Strain S95(T) grew anaerobically with elemental sulfur as an energy source and bicarbonate/CO2 as a carbon source. Elemental sulfur was disproportionated to sulfide and sulfate. Growth was enhanced in the presence of poorly crystalline iron(III) oxide (ferrihydrite) as a sulfide-scavenging agent. Strain S95(T) was also able to grow by disproportionation of thiosulfate and sulfite. Sulfate was not used as an electron acceptor. Analysis of the 16S rRNA gene sequence revealed that the isolate belongs to the phylum Thermodesulfobacteria. On the basis of its physiological properties and results of phylogenetic analyses, it is proposed that the isolate represents the sole species of a new genus, Thermosulfurimonas dismutans gen. nov., sp. nov.; S95(T) (=DSM 24515(T)=VKM B-2683(T)) is the type strain of the type species. This is the first description of a thermophilic microorganism that disproportionates elemental sulfur.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1466-5026},
	doi = {10.1099/ijs.0.034397-0},
	author = {Slobodkin, A I and Reysenbach, A -L. and Slobodkina, G B and Baslerov, R V and Kostrikina, N A and Wagner, I D and Bonch-Osmolovskaya, E A}
}
@article {ISI:000308207400009,
	title = {{The Transcriptome of Bathymodiolus azoricus Gill Reveals Expression of Genes from Endosymbionts and Free-Living Deep-Sea Bacteria}},
	journal = {MARINE DRUGS},
	volume = {10},
	number = {8},
	year = {2012},
	month = {aug},
	pages = {1765{\textendash}1783},
	type = {Article},
	abstract = {Deep-sea environments are largely unexplored habitats where a surprising number of species may be found in large communities, thriving regardless of the darkness, extreme cold, and high pressure. Their unique geochemical features result in reducing environments rich in methane and sulfides, sustaining complex chemosynthetic ecosystems that represent one of the most surprising findings in oceans in the last 40 years. The deep-sea Lucky Strike hydrothermal vent field, located in the Mid Atlantic Ridge, is home to large vent mussel communities where Bathymodiolus azoricus represents the dominant faunal biomass, owing its survival to symbiotic associations with methylotrophic or methanotrophic and thiotrophic bacteria. The recent transcriptome sequencing and analysis of gill tissues from B. azoricus revealed a number of genes of bacterial origin, hereby analyzed to provide a functional insight into the gill microbial community. The transcripts supported a metabolically active microbiome and a variety of mechanisms and pathways, evidencing also the sulfur and methane metabolisms. Taxonomic affiliation of transcripts and 16S rRNA community profiling revealed a microbial community dominated by thiotrophic and methanotrophic endosymbionts of B. azoricus and the presence of a Sulfurovum-like epsilonbacterium.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1660-3397},
	doi = {10.3390/md10081765},
	author = {Egas, Conceicao and Pinheiro, Miguel and Gomes, Paula and Barroso, Cristina and Bettencourt, Raul}
}
@article {Rubin2012,
	title = {{Volcanic Eruptions in the Deep Sea}},
	journal = {Oceanography},
	volume = {25},
	number = {1},
	year = {2012},
	month = {mar},
	pages = {142{\textendash}157},
	publisher = {OCEANOGRAPHY SOC},
	address = {ROCKVILLE; P.O. BOX 1931, ROCKVILLE, MD USA},
	abstract = {Volcanic eruptions are important events in Earth{\textquoteright}s cycle of magma generation and crustal construction. Over durations of hours to years, eruptions produce new deposits of lava and/or fragmentary ejecta, transfer heat and magmatic volatiles from Earth{\textquoteright}s interior to the overlying air or seawater, and significantly modify the landscape and perturb local ecosystems. Today and through most of geological history, the greatest number and volume of volcanic eruptions on Earth have occurred in the deep ocean along mid-ocean ridges, near subduction zones, on oceanic plateaus, and on thousands of mid-plate seamounts. However, deep-sea eruptions ({\textgreater} 500~m depth) are much more difficult to detect and observe than subaerial eruptions, so comparatively little is known about them. Great strides have been made in eruption detection, response speed, and observational detail since the first recognition of a deep submarine eruption at a mid-ocean ridge 25 years ago. Studies of ongoing or recent deep submarine eruptions reveal information about their sizes, durations, frequencies, styles, and environmental impacts. Ultimately, magma formation and accumulation in the upper mantle and crust, plus local tectonic stress fields, dictate when, where, and how often submarine eruptions occur, whereas eruption depth, magma composition, conditions of volatile segregation, and tectonic setting determine submarine eruption style.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {1042-8275},
	doi = {10.5670/oceanog.2012.12},
	author = {Rubin, K H and Soule, S A and Chadwick, W W and Fornari, D J and Clague, D A and Embley, R W and Baker, E T and Perfit, M R and Caress, D W and Dziak, R P}
}
@article {Thiel2012,
	title = {{Widespread occurrence of two carbon fixation pathways in tubeworm endosymbionts: lessons from hydrothermal vent associated tubeworms from the Mediterranean Sea}},
	journal = {Frontiers in Microbiology},
	volume = {2},
	year = {2012},
	pages = {423},
	abstract = {Vestimentiferan tubeworms (siboglinid polychetes) of the genus Lamellibrachia are common members of cold seep faunal communities and have also been found at sedimented hydrothermal vent sites in the Pacific. As they lack a digestive system, they are nourished by chemoautotrophic bacterial endosymbionts growing in a specialized tissue called the trophosome. Here we present the results of investigations of tubeworms and endosymbionts from a shallow hydrothermal vent field in the Western Mediterranean Sea. The tubeworms, which are the first reported vent-associated tubeworms outside the Pacific, are identified as Lamellibrachia anaximandri using mitochondrial ribosomal and cytochrome oxidase I (COI) gene sequences. They harbor a single gammaproteobacterial endosymbiont. Carbon isotopic data, as well as the analysis of genes involved in carbon and sulfur metabolism indicate a sulfide-oxidizing chemoautotrophic endosymbiont. The detection of a hydrogenase gene fragment suggests the potential for hydrogen oxidation as alternative energy source. Surprisingly, the endosymbiont harbors genes for two different carbon fixation pathways, the Calvin-Benson-Bassham (CBB) cycle as well as the reductive tricarboxylic acid (rTCA) cycle, as has been reported for the endosymbiont of the vent tubeworm Riftia pachyptila. In addition to RubisCO genes we detected ATP citrate lyase (ACL {\textendash} the key enzyme of the rTCA cycle) type II gene sequences using newly designed primer sets. Comparative investigations with additional tubeworm species (Lamellibrachia luymesi, Lamellibrachia sp. 1, Lamellibrachia sp. 2, Escarpia laminata, Seepiophila jonesi) from multiple cold seep sites in the Gulf of Mexico revealed the presence of acl genes in these species as well. Thus, our study suggests that the presence of two different carbon fixation pathways, the CBB cycle and the rTCA cycle, is not restricted to the Riftia endosymbiont, but rather might be common in vestimentiferan tubeworm endosymbionts, regardless of the habitat.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.3389/fmicb.2012.00423},
	author = {Thiel, Vera and Hugler, M and Bl{\"u}mel, M and Baumann, H I and G{\"a}rtner, A and Schmaljohann, R and Strauss, H and Garbe-Schonberg, D and Petersen, S and Cowart, D A and Fisher, C R and Imhoff, J F}
}
@article {ISI:000295971300003,
	title = {{Bacterial sulfur cycling shapes microbial communities in surface sediments of an ultramafic hydrothermal vent field}},
	journal = {ENVIRONMENTAL MICROBIOLOGY},
	volume = {13},
	number = {10},
	year = {2011},
	month = {oct},
	pages = {2633{\textendash}2648},
	type = {Article},
	abstract = {The ultramafic-hosted Logatchev hydrothermal field (LHF) is characterized by vent fluids, which are enriched in dissolved hydrogen and methane compared with fluids from basalt-hosted systems. Thick sediment layers in LHF are partly covered by characteristic white mats. In this study, these sediments were investigated in order to determine biogeochemical processes and key organisms relevant for primary production. Temperature profiling at two mat-covered sites showed a conductive heating of the sediments. Elemental sulfur was detected in the overlying mat and metal-sulfides in the upper sediment layer. Micro-profiles revealed an intensive hydrogen sulfide flux from deeper sediment layers. Fluorescence in situ hybridization showed that filamentous and vibrioid, Arcobacter-related Epsilonproteobacteria dominated the overlying mats. This is in contrast to sulfidic sediments in basalt-hosted fields where mats of similar appearance are composed of large sulfur-oxidizing Gammaproteobacteria. Epsilonproteobacteria (7-21{\%}) and Deltaproteobacteria (20-21{\%}) were highly abundant in the surface sediment layer. The physiology of the closest cultivated relatives, revealed by comparative 16S rRNA sequence analysis, was characterized by the capability to metabolize sulfur components. High sulfate reduction rates as well as sulfide depleted in (34)S further confirmed the importance of the biogeochemical sulfur cycle. In contrast, methane was found to be of minor relevance for microbial life in mat-covered surface sediments. Our data indicate that in conductively heated surface sediments microbial sulfur cycling is the driving force for bacterial biomass production although ultramafichosted systems are characterized by fluids with high levels of dissolved methane and hydrogen.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1462-2912},
	doi = {10.1111/j.1462-2920.2011.02530.x},
	author = {Schauer, Regina and Roy, Hans and Augustin, Nico and Gennerich, Hans-Hermann and Peters, Marc and Wenzhoefer, Frank and Amann, Rudolf and Meyerdierks, Anke}
}
@article {Birgel2011,
	title = {{Changing redox conditions at cold seeps as revealed by authigenic carbonates from Alaminos Canyon, northern Gulf of Mexico}},
	journal = {Chemical Geology},
	volume = {285},
	number = {1-4},
	year = {2011},
	pages = {82{\textendash}96},
	abstract = {Anaerobic oxidation of methane in anoxic sediments at cold seeps often leads to formation of authigenic carbonates close to the seafloor along continental margins. Recent work, however, indicated that the redox conditions in sediments may vary to some degree during seepage activity. In order to shed new light on the extent of this variability, authigenic carbonates from Alaminos Canyon lease block 645 of the northern Gulf of Mexico have been characterized by means of inorganic and organic geochemistry. The carbonates were collected from seep deposits representing various seafloor morphologies, including extensive pavements, mounds, fractured carbonate slabs surrounded by dense bivalve shells, and vestimentiferan tubeworm colonies. The deposits almost entirely consist of aragonite. The $δ$18O values of aragonite vary from +~2.6 to +~5.8{\textperthousand} V-PDB, suggesting precipitation in slight disequilibrium with the surrounding pore fluids. The $δ$13C values of aragonite between -~33.9 and -~20.4{\textperthousand} V-PDB agree with variable amounts of carbonate derived from oxidation of thermogenic methane and crude oil. Methane was primarily oxidized in an anaerobic process as revealed by the presence of 13C-depleted molecular fossils of methane-oxidizing archaea ($δ$13C values as negative as -~118{\textperthousand}) and sulfate-reducing bacteria ($δ$13C values as negative as -~97{\textperthousand}), the syntrophic partners in the anaerobic oxidation of methane. The observed inventories of molecular fossils in the authigenic carbonates mirror those of known consortia of anaerobic methane oxidizing archaea (ANME) and sulfate-reducing bacteria, namely the ANME-2/Desulfosarcina/Desulfococcus (DSS) and ANME-3/Desulfobulbus (DBB) consortia. In contrast, the same carbonates exhibit shale-normalized rare earth elements patterns that all display real negative Ce anomalies (Ce/Ce*~ In contrast, the same carbonates exhibit shale-normalized rare earth elements patterns that all display real negative Ce anomalies (Ce/Ce*~13C values of the biomarkers of aerobic methanotrophs are as negative as -~58{\textperthousand} and are consequently less 13C-depleted than the molecular fossils of the prokaryotes performing anaerobic oxidation of methane, a pattern in accord with culture experiments. Overall, our results suggest that redox conditions at cold seeps are variable. This variability probably reflects changes in seepage flux. The combination of an inorganic and an organic geochemical approach used here is promising to better assess the variability and diversity of past fluid and gas expulsion at seeps.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0009-2541},
	doi = {10.1016/j.chemgeo.2011.03.004},
	url = {http://dx.doi.org/10.1016/j.chemgeo.2011.03.004},
	author = {Birgel, D and Feng, D and Roberts, H H and Peckmann, J}
}
@article {Mottl2011,
	title = {{Chemistry of hot springs along the Eastern Lau Spreading Center}},
	journal = {Geochimica et Cosmochimica Acta},
	volume = {75},
	number = {4},
	year = {2011},
	pages = {1013{\textendash}1038},
	abstract = {The Eastern Lau Spreading Center (ELSC) is the southernmost part of the back-arc spreading axis in the Lau Basin, west of the Tonga trench and the active Tofua volcanic arc. Over its 397-km length it exhibits large and systematic changes in spreading rate, magmatic/tectonic processes, and proximity to the volcanic arc. In 2005, we collected 81 samples of vent water from six hydrothermal fields along the ELSC. The chemistry of these waters varies both within and between vent fields, in response to changes in substrate composition, temperature and pressure, pH, water/rock ratio, and input from magmatic gases and subducted sediment. Hot-spring temperatures range from 229{\textdegree} to 363~{\textdegree}C at the five northernmost fields, with a general decrease to the south that is reversed at the Mariner field. The southernmost field, Vai Lili, emitted water at up to 334~{\textdegree}C in 1989 but had a maximum venting temperature of only 121~{\textdegree}C in 2005, due to waning activity and admixture of bottom seawater into the subseafloor plumbing system. Chloride varies both within fields and from one field to another, from a low of 528~mmol/kg to a high of 656~mmol/kg, and may be enriched by phase separation and/or leaching of Cl from the rock. Concentrations of the soluble elements K, Rb, Cs, and B likewise increase southward as the volcanic substrate becomes more silica-rich, especially on the Valu Fa Ridge. Iodine and $δ$7Li increase southward, and $δ$11B decreases as B increases, apparently in response to increased input from subducted sediment as the arc is approached. Species that decrease southward as temperature falls are Si, H2S, Li, Na/Cl, Fe, Mn, and 87Sr/86Sr, whereas pH, alkalinity, Ca, and Sr increase. Oxygen isotopes indicate a higher water/rock ratio in the three systems on Valu Fa Ridge, consistent with higher porosity in more felsic volcanic rocks. Vent waters at the Mariner vent field on the Valu Fa Ridge are significantly hotter, more acid and metal-rich, less saline, and richer in dissolved gases and other volatiles, including H2S, CO2, and F, than the other vent fields, consistent with input of magmatic gases. The large variations in geologic and geophysical parameters produced by back-arc spreading along the ELSC, which exceed those along mid-ocean ridge spreading axes, produce similar large variations in the composition of vent waters, and thus provide new insights into the processes that control the chemistry of submarine hot springs.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0016-7037},
	doi = {10.1016/j.gca.2010.12.008},
	url = {http://dx.doi.org/10.1016/j.gca.2010.12.008},
	author = {Mottl, M J and Seewald, J S and Wheat, C G and Tivey, M K and Michael, P J and Proskurowski, G and McCollom, T M and Reeves, E and Sharkey, J and You, C F and Chan, L H and Pichler, T}
}
@article {Gieskes2011,
	title = {{Cold seeps in Monterey Bay, California: Geochemistry of pore waters and relationship to benthic foraminiferal calcite}},
	journal = {Applied Geochemistry},
	volume = {26},
	number = {5},
	year = {2011},
	pages = {738{\textendash}746},
	abstract = {An extensive geochemical and biogeochemical examination of CH4 seeps in the Clam Flats area of Monterey Bay provides insight into the character of relationships between seep geochemistry and benthic foraminiferal geochemistry. The area is characterized by sulfide-rich fluids. Sulfide increases are associated with large increases in alkalinity, as well as small decreases in dissolved Ca and Mg. In addition, only small increases in NH4 are observed, but values of $δ$13C of dissolved inorganic C are as low as -60{\textperthousand} at shallow depths (C of dissolved inorganic C are as low as -60{\textperthousand} at shallow depths (4, which is transported upward by slow seepage of pore fluids. The geochemistry of the pore fluids should be relevant to the geochemistry of the carbonate tests of living and dead foraminifera. However, a profound disequilibrium of approximately an order of magnitude occurs between the $δ$13C values of stained (cytoplasm-containing) foraminiferal carbonate and the C isotope values of ambient pore water dissolved inorganic C. Reasons are unclear for this isotopic disequilibrium, but have important implications for interpretations of foraminiferal carbonate as a paleoenvironmental proxy. Much fine scale work is needed to fully understand the relationships between the biogeochemistry of benthic foraminifera and the geochemistry of the pore waters where they live.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0883-2927},
	doi = {10.1016/j.apgeochem.2011.01.032},
	url = {http://dx.doi.org/10.1016/j.apgeochem.2011.01.032},
	author = {Gieskes, J M and Rathburn, A E and Martin, J B and Perez, M E and Mahn, C and Bernhard, J M and Day, S}
}
@article {ISI:000292610200001,
	title = {{Deciphering the ocean bottom pressure variation in the Logatchev hydrothermal field at the eastern flank of the Mid-Atlantic Ridge}},
	journal = {GEOCHEMISTRY GEOPHYSICS GEOSYSTEMS},
	volume = {12},
	year = {2011},
	month = {jul},
	type = {Article},
	abstract = {Ocean bottom pressure data from the Logatchev hydrothermal field (LHF) are presented and analyzed. The data were collected with two ocean bottom pressure meters (OBPs), constructed at the University of Bremen, that are capable of recording signals with frequencies up to 0.25 Hz. Over the long-term, a nearly 2.5 kPa (25 cm water column equivalent) pressure variation over 3.7 years is observed, which is consistent with uplift followed by subsidence, but cannot unequivocally be discerned from instrumental drift. Medium-term pressure variations are compared with satellite surface topography, satellite gravity, ocean modeling, and in situ data from an OBP 700 km away. It is shown that fluctuations in the oceanic mass distribution dominate the variations in this frequency range and that oceanic modeling and data from a 700 km distant OBP are positively correlated with the LHF bottom pressure time series. The short-term variations are dominated by microseisms originating from sea surface waves and pressure waves from earthquakes as can be shown by comparison with weather buoy and teleseismic data.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1525-2027},
	doi = {10.1029/2010GC003441},
	author = {Gennerich, Hans-Hermann and Villinger, Heinrich}
}
@article {ISI:000298301200007,
	title = {{Effects of chronic low carbonate saturation levels on the distribution, growth and skeletal chemistry of deep-sea corals and other seamount megabenthos}},
	journal = {MARINE ECOLOGY PROGRESS SERIES},
	volume = {442},
	year = {2011},
	pages = {87{\textendash}99},
	type = {Article},
	abstract = {Ocean acidification has been predicted to reduce the ability of marine organisms to produce carbonate skeletons, threatening their long-term viability and severely impacting marine ecosystems. Corals, as ecosystem engineers, have been identified as particularly vulnerable and important. To determine the sensitivity of corals and allied taxa to long-term exposure to very low carbonate concentrations, we examined the distribution and skeletal characteristics of coral taxa along a natural deep-sea concentration gradient on seamounts of SW Australia. Carbonate under-saturation had little evident effect on the depth distribution, growth or skeletal composition of live scleractinians or gorgonians, with corals growing, often abundantly, in waters as much as 20 to 30{\%} under-saturated. Developmental anomalies in the deepest skeleton-forming anthozoan collected (an isidid gorgonian, at nearly 4 km depth) suggest an absolute low tolerance limit of about 40{\%} under-saturation. Evidence for an effect of acidification on the accumulation of reef structure is ambiguous, with clear indications of dissolution of high-magnesium calcite (HMC) gorgonian skeletons at depths below 2300 m, but also abundant, old scleractinian skeletons well below the aragonite saturation horizon. The latter might be the result of ferromanganese deposition on exposed skeletons, which, however, may render them inhospitable for benthic organisms.},
	keywords = {AUV ABE (Autonomous Benthic Explorer), ROV Jason (Remotely Operated Vehicle)},
	issn = {0171-8630},
	doi = {10.3354/meps09400},
	author = {Thresher, Ronald E and Tilbrook, Bronte and Fallon, Stewart and Wilson, Nick C and Adkins, Jess}
}
@article {ISI:000296056300001,
	title = {{Extraordinarily high biomass benthic community on Southern Ocean seamounts}},
	journal = {SCIENTIFIC REPORTS},
	volume = {1},
	year = {2011},
	month = {oct},
	type = {Article},
	abstract = {We describe a previously unknown assemblage of seamount-associated megabenthos that has by far the highest peak biomass reported in the deep-sea outside of vent communities. The assemblage was found at depths of 2-2.5 km on rocky geomorphic features off the southeast coast of Australia, in an area near the Sub-Antarctic Zone characterised by high rates of surface productivity and carbon export to the deep-ocean. These conditions, and the taxa in the assemblage, are widely distributed around the Southern mid-latitudes, suggesting the high-biomass assemblage is also likely to be widespread. The role of this assemblage in regional ecosystem and carbon dynamics and its sensitivities to anthropogenic impacts are unknown. The discovery highlights the lack of information on deep-sea biota worldwide and the potential for unanticipated impacts of deep-sea exploitation.},
	keywords = {AUV ABE (Autonomous Benthic Explorer), ROV Jason (Remotely Operated Vehicle)},
	issn = {2045-2322},
	doi = {10.1038/srep00119},
	author = {Thresher, R E and Adkins, J and Fallon, S J and Gowlett-Holmes, K and Althaus, F and Williams, A}
}
@article {Schmidt2011,
	title = {{Fluid elemental and stable isotope composition of the Nibelungen hydrothermal field (8 degrees18{\textquoteright}S, Mid-Atlantic Ridge): Constraints on fluid-rock interaction in heterogeneous lithosphere}},
	journal = {Chemical Geology},
	volume = {280},
	number = {1-2},
	year = {2011},
	pages = {1{\textendash}18},
	abstract = {Depending on the geological setting, the interaction of submarine hydrothermal fluids with the host rock leads to distinct energy and mass transfers between the lithosphere and the hydrosphere. The Nibelungen hydrothermal field is located at 8{\textdegree}18'S, about 9~km off-axis of the Mid-Atlantic Ridge (MAR). At 3000~m water depth, 372~{\textdegree}C hot, acidic fluids emanate directly from the bottom, without visible sulfide chimney formation. Hydrothermal fluids obtained in 2009 are characterized by low H2S concentrations (1.1~mM), a depletion of B (192~$μ$M) relative to seawater, lower Si (13.7~mM) and Li (391~$μ$M) concentrations relative to basaltic-hosted hydrothermal systems and a large positive Eu anomaly, and display a distinct stable isotope signature of hydrogen (∆2HH2O~=~7.6{\textendash}8.7{\textperthousand}) and of oxygen (∆18OH2O~=~2.2{\textendash}2.4{\textperthousand}). The heavy hydrogen isotopic signature of the Nibelungen fluids is a specific feature of ultramafic-hosted hydrothermal systems and is mainly controlled by the formation of OH-bearing alteration minerals like serpentine, brucite, and tremolite during pervasive serpentinization. New isotopic data obtained for the ultramafic-hosted Logatchev I field at 14{\textdegree}45'N, MAR (∆2HH2O~=~3.8{\textendash}4.2{\textperthousand}) display a similar trend, being clearly distinguished from other, mafic-hosted hydrothermal systems at the MAR. The fluid geochemistry at Nibelungen kept stable since the first sampling campaign in 2006 and is evident for a hybrid alteration of mafic and ultramafic rocks in the subseafloor. Whereas the ultramafic-fingerprint parameters Si, Li, B, Eu anomaly and ∆2HH2O distinguish the Nibelungen field from other hydrothermal systems venting in basaltic settings at similar physico-chemical conditions and are related to the interaction with mantle rocks, the relatively high concentrations of trace alkali elements, Pb, and Tl can only be attributed to the alteration of melt-derived gabbroic rocks. The elemental and isotopic composition of the fluid suggest a multi-step alteration sequence: (1) low- to medium-temperature alteration of gabbroic rocks, (2) pervasive serpentinization at moderate to high temperatures, and (3) limited high-temperature interaction with basaltic rocks during final ascent of the fluid. The integrated water/rock ratio for the Nibelungen hydrothermal system is about 0.5. The fluid compositional fingerprint at Nibelungen is similar to the ultramafic-hosted Logatchev I fluids with respect to key parameters. Some compositional differences can be ascribed to different alteration temperatures and other fluid pathways involving a variety of source rocks, higher water/rock ratios, and sulfide precipitation in the sub-seafloor at Logatchev I.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0009-2541},
	doi = {10.1016/j.chemgeo.2010.07.008},
	url = {http://dx.doi.org/10.1016/j.chemgeo.2010.07.008},
	author = {Schmidt, K and Garbe-Schonberg, D and Koschinsky, A and Strauss, H and Jost, C L and Klevenz, V and Koniger, P}
}
@article {Reeves2011,
	title = {{Geochemistry of hydrothermal fluids from the PACMANUS, Northeast Pual and Vienna Woods hydrothermal fields, Manus Basin, Papua New Guinea}},
	journal = {Geochimica et Cosmochimica Acta},
	volume = {75},
	number = {4},
	year = {2011},
	pages = {1088{\textendash}1123},
	abstract = {Processes controlling the composition of seafloor hydrothermal fluids in silicic back-arc or near-arc crustal settings remain poorly constrained despite growing evidence for extensive magmatic{\textendash}hydrothermal activity in such environments. We conducted a survey of vent fluid compositions from two contrasting sites in the Manus back-arc basin, Papua New Guinea, to examine the influence of variations in host rock composition and magmatic inputs (both a function of arc proximity) on hydrothermal fluid chemistry. Fluid samples were collected from felsic-hosted hydrothermal vent fields located on Pual Ridge (PACMANUS and Northeast (NE) Pual) near the active New Britain Arc and a basalt-hosted vent field (Vienna Woods) located farther from the arc on the Manus Spreading Center. Vienna Woods fluids were characterized by relatively uniform endmember temperatures (273{\textendash}285~{\textdegree}C) and major element compositions, low dissolved CO2 concentrations (4.4~mmol/kg) and high measured pH (4.2{\textendash}4.9 at 25~{\textdegree}C). Temperatures and compositions were highly variable at PACMANUS/NE Pual and a large, newly discovered vent area (Fenway) was observed to be vigorously venting boiling (358~{\textdegree}C) fluid. All PACMANUS fluids are characterized by negative $δ$DH2O$δ$DH2O values, in contrast to positive values at Vienna Woods, suggesting substantial magmatic water input to circulating fluids at Pual Ridge. Low measured pH (25~{\textdegree}C) values (\~{}2.6{\textendash}2.7), high endmember CO2 (up to 274~mmol/kg) and negative $δ$34SH2S$δ$34SH2S values (down to -2.7{\textperthousand}) in some vent fluids are also consistent with degassing of acid-volatile species from evolved magma. Dissolved CO2 at PACMANUS is more enriched in 13C (-4.1{\textperthousand} to -2.3{\textperthousand}) than Vienna Woods (-5.2{\textperthousand} to -5.7{\textperthousand}), suggesting a contribution of slab-derived carbon. The mobile elements (e.g. Li, K, Rb, Cs and B) are also greatly enriched in PACMANUS fluids reflecting increased abundances in the crust there relative to the Manus Spreading Center. Variations in alkali and dissolved gas abundances with Cl at PACMANUS and NE Pual suggest that phase separation has affected fluid chemistry despite the low temperatures of many vents. In further contrast to Vienna Woods, substantial modification of PACMANUS/NE Pual fluids has taken place as a result of seawater ingress into the upflow zone. Consistently high measured Mg concentrations as well as trends of increasingly non-conservative SO4 behavior, decreasing endmember Ca/Cl and Sr/Cl ratios with increased Mg indicate extensive subsurface anhydrite deposition is occurring as a result of subsurface seawater entrainment. Decreased pH and endmember Fe/Mn ratios in higher Mg fluids indicate that the associated mixing/cooling gives rise to sulfide deposition and secondary acidity production. Several low temperature (⩽80~{\textdegree}C) fluids at PACMANUS/NE Pual also show evidence for anhydrite dissolution and water{\textendash}rock interaction (fixation of B) subsequent to seawater entrainment. Hence, the evolution of fluid compositions at Pual Ridge reflects the cumulative effects of water/rock interaction, admixing and reaction of fluids exsolved from silicic magma, phase separation/segregation and seawater ingress into upflow zones.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0016-7037},
	doi = {10.1016/j.gca.2010.11.008},
	url = {http://dx.doi.org/10.1016/j.gca.2010.11.008},
	author = {Reeves, E and Seewald, J S and Saccocia, P J and Walsh, E and Bach, W and Craddock, P R and Shanks, W C and Sylva, S P and Pichler, T and Rosner, M}
}
@article {Yucel2011,
	title = {{Hydrothermal vents as a kinetically stable source of iron-sulphide-bearing nanoparticles to the ocean}},
	journal = {Nature Geoscience},
	volume = {4},
	number = {6},
	year = {2011},
	month = {jun},
	pages = {367{\textendash}371},
	publisher = {NATURE PUBLISHING GROUP},
	address = {NEW YORK; 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA},
	abstract = {Hydrothermal vents emit sulphur and metals to the ocean(1). Particular attention has been paid to hydrothermal fluxes of iron(2-4), a limiting micronutrient of marine primary production(5). Vent-derived iron was previously thought to rapidly oxidize and precipitate around vents(6). However, organic matter can bind to and stabilize dissolved and particulate iron in hydrothermal plumes(7-9), facilitating its dispersion into the open ocean(10). Here, we report measurements of the chemical speciation of sulphide and iron in high-temperature fluids emanating from vents in the East Pacific Rise and the Eastern Lau Spreading Center. We show that pyrite nanoparticles-composed of iron and sulphur-account for up to 10{\%} of the filterable iron (less than 200nm in size) in these fluids. We suggest that these particles form before the discharge of the vent fluid. We estimate that pyrite nanoparticles sink more slowly than larger plume particles, and are more resistant to oxidation than dissolved Fe(II) and FeS. We suggest that the discharge of iron in the form of pyrite nanoparticles increases the probability that vent-derived iron will be transported over long distances in the deep ocean.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {1752-0894},
	doi = {10.1038/NGEO1148},
	author = {Yucel, M and Gartman, A and Chan, Clara S and Luther, G W}
}
@article {ISI:000295134600001,
	title = {{In situ measurements of hydrogen sulfide, oxygen, and temperature in diffuse fluids of an ultramafic-hosted hydrothermal vent field (Logatchev, 14 degrees 45 {\textquoteleft} N, Mid-Atlantic Ridge): Implications for chemosymbiotic bathymodiolin mussels}},
	journal = {GEOCHEMISTRY GEOPHYSICS GEOSYSTEMS},
	volume = {12},
	year = {2011},
	month = {sep},
	type = {Article},
	abstract = {The Logatchev hydrothermal vent field (14 degrees 45{\textquoteright}N, Mid-Atlantic Ridge) is located in a ridge segment characterized by mantle-derived ultramafic outcrops. Compared to basalt-hosted vents, Logatchev high-temperature fluids are relatively low in sulfide indicating that the diffuse, low-temperature fluids of this vent field may not contain sufficient sulfide concentrations to support a chemosymbiotic invertebrate community. However, the high abundances of bathymodiolin mussels with bacterial symbionts related to free-living sulfur-oxidizing bacteria suggested that bioavailable sulfide is present at Logatchev. To clarify, if diffuse fluids above mussel beds of Bathymodiolus puteoserpentis provide the reductants and oxidants needed by their symbionts for aerobic sulfide oxidation, in situ microsensor measurements of dissolved hydrogen sulfide and oxygen were combined with simultaneous temperature measurements. High temporal fluctuations of all three parameters were measured above the mussel beds. H2S and O-2 coexisted with mean concentrations between 9 and 31 mu M (H2S) and 216 and 228 mu M (O-2). Temperature maxima ({\textless}= 7.4 degrees C) were generally concurrent with H2S maxima ({\textless}= 156 mu M) and O-2 minima ({\textgreater}= 142 mu M). Long-term measurements for 250 days using temperature as a proxy for oxygen and sulfide concentrations indicated that the mussels were neither oxygen limited nor sulfide limited. Our in situ measurements at Logatchev indicate that sulfide may also be bioavailable in diffuse fluids from other ultramafic-hosted vents along slow and ultraslow spreading ridges. Components: 12,100 words, 9 figures, 4 tables.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1525-2027},
	doi = {10.1029/2011GC003632},
	author = {Zielinski, Frank U and Gennerich, Hans-Hermann and Borowski, Christian and Wenzhoefer, Frank and Dubilier, Nicole}
}
@article {ISI:000296085300022,
	title = {{Modal analysis of the deep-water solitary scleractinian, Desmophyllum dianthus, on SW Pacific seamounts: inferred recruitment periodicity, growth, and mortality rates}},
	journal = {CORAL REEFS},
	volume = {30},
	number = {4},
	year = {2011},
	month = {dec},
	pages = {1063{\textendash}1070},
	type = {Article},
	abstract = {Little is known about the demography of corals inhabiting deep-sea features due to the logistical difficulties of working at the extreme depths they inhabit. To obtain basic information about growth, mortality, and recruitment dynamics for such a coral, we applied modal analysis to the size frequency distributions of live-caught and sub-fossil specimens of the widely distributed solitary cup coral, Desmophyllum dianthus, collected on SW Pacific seamounts. Comparison of live-caught material collected in 1997 and 2007-2009 indicated modal progression over time and an implied maximum age of approximately 190 years, which is similar to ages determined previously for D. dianthus using radiometric techniques. A log-linear decline in the number of individuals with increasing size further implies a constant adult mortality rate, of 15.1{\%} per annum in 1997 and 9.2{\%} per annum in 2007-2009. The spacing of size modes in the 2007-2009 samples suggests regularly episodic recruitment events, at 22- to 32-year intervals, which may relate to periodic variability in large-scale Southern Ocean circulation. Preliminary analyses of size frequency distributions of the sub-fossil material suggest that the trophodynamics, growth, and adult mortality schedules of D. dianthus in the SW Pacific have remained basically similar throughout the Holocene.},
	keywords = {AUV ABE (Autonomous Benthic Explorer), ROV Jason (Remotely Operated Vehicle)},
	issn = {0722-4028},
	doi = {10.1007/s00338-011-0806-7},
	author = {Thresher, R E and Adkins, J and Thiagarajan, N}
}
@article {ISI:000299425400001,
	title = {{Nautiliniellidae (Annelida) from Costa Rican cold seeps and a western Pacific hydrothermal vent, with description of four new species}},
	journal = {SYSTEMATICS AND BIODIVERSITY},
	volume = {9},
	number = {2},
	year = {2011},
	pages = {109{\textendash}131},
	type = {Article},
	abstract = {Four genera and five species of Nautiliniellidae (Phyllodocida; Annelida), representing four new species and one new record, were found in association with bivalves belonging to Mytilidae (Bathymodiolus spp.), Solemyidae (Acharax sp.) and Vesicomyidae (Calyptogena and Vesicomya) from hydrothermal vents of the western Pacific (Lau back-arc basin) and cold seeps of the eastern Pacific (Costa Rica). Iheyomytilidicola lauensis n. sp. is characterized by the presence of unidentate hooks; Laubierus alvini n. sp. has long dorsal cirri and notoacicula not protruding in any segment; Natsushima sashai n. sp has bifurcate chaetae with distal teeth markedly different in length and width between each other; and Shinkai fontefridae n. sp. differs in having posterior hooks with subdistal spines. Sexual dimorphism is described for Shinkai and Natsushima species and confirmed by DNA sequencing (Cytochrome c oxidase subunit I sequences). In addition, one specimen of N. bifurcata Miura {\&} Laubier, 1990 from the Gulf of Cadiz has been studied to compare it morphologically and genetically with N. sashai n. sp. Scanning electron and optical microscopy images are provided for each species, as well as pictures of live animals. Morphological features described and documented for the first time include the presence of internal longitudinal chambers within chaetae and possible evidence that the simple chaetae, characteristic of nautiliniellids, may be derived from compound chaetae.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1477-2000},
	doi = {10.1080/14772000.2011.569033},
	author = {Teresa Aguado, M and Rouse, Greg W}
}
@article {Deardorff2011,
	title = {{Observations of eruptive plume dynamics and pyroclastic deposits from submarine explosive eruptions at NW Rota-1, Mariana arc}},
	journal = {Journal of Volcanology and Geothermal Research},
	volume = {202},
	number = {1-2},
	year = {2011},
	pages = {47{\textendash}59},
	abstract = {Observations at the submarine volcano NW Rota-1, Mariana arc, have provided a unique opportunity to study basaltic explosive eruptions at {\~{}}~550~m below sea level. In particular, during one week of observations in 2006, the active vent evolved from a region of diffuse gas venting and slow lava effusion to a focused vent that emitted a nearly continuous stream of gas with entrained juvenile pyroclasts. The eruptions were small, strombolian bursts that ejected bomb-sized pyroclasts meters above the vent and produced buoyant plumes that rose tens of meters above the vent and contained variable amounts of ash. Sampled pyroclasts are dominantly coarse ash and lapilli with fluidal textures that provide evidence of magmatic fragmentation. However, the blocky morphologies of some clasts, combined with visual evidence of secondary fragmentation, suggest that quench granulation due to rapid cooling was also important in pyroclast formation. Rapid cooling is also suggested by the slow rise of buoyant plumes (which indicates a low heat content and small density contrast with surrounding seawater). Rapid plume deceleration in seawater limited deposition of coarse ash, lapilli, and bombs to within a few meters of the vent, and allowed recycling of <={\~{}}15{\%} of the ejected clasts. Petrologic evidence for pyroclast recycling is provided by numerous microcrystalline inclusions incorporated within microlite-poor matrix glass. The matrix glass within these inclusions is strongly enriched in chlorine (},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0377-0273},
	doi = {10.1016/j.jvolgeores.2011.01.003},
	url = {http://dx.doi.org/10.1016/j.jvolgeores.2011.01.003},
	author = {Deardorff, N D and Cashman, K V and Chadwick, W W}
}
@article {ISI:000290596200008,
	title = {{Organization and Microanatomy of the Sclerolinum contortum Trophosome (Polychaeta, Siboglinidae)}},
	journal = {BIOLOGICAL BULLETIN},
	volume = {220},
	number = {2},
	year = {2011},
	month = {apr},
	pages = {140{\textendash}153},
	type = {Article},
	abstract = {The trophosome-an organ especially evolved to accommodate symbiotic bacteria-is a key character of the polychaete family Siboglinidae. Astonishingly, the trophosomes vary in organization and origin between the different siboglinid taxa. The trophosome of the small genus Sclerolinum was nearly unknown until now. Here we investigated the trophosome of S. contortum from the Gulf of Mexico, using light and electron microscopy. We show that this organ derives from the visceral mesoderm and propose that the trophosome of the sister clade Vestimentifera and Sclerolinum is a homologous character. Like that of juvenile vestimentiferans, the trophosome of Sclerolinum trophosome is simply organized. This study reveals that the Sclerolinum trophosome exhibits two regions that differ in the organization of host tissue and the size and shape of the symbionts. We suggest that a specific cell cycle within the symbiont-housing organ is directed along the longitudinal body axis, with a region of proliferation anteriorly and a region of degradation posteriorly. Using Raman microspectroscopy we demonstrate that the endosymbionts of S. contortum from the Gulf of Mexico contain sulfur vesicles, and we argue for a chemoautotrophic sulfur-oxidizing metabolism.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0006-3185},
	author = {Eichinger, Irmgard and Klepal, Waltraud and Schmid, Markus and Bright, Monika}
}
@article {ISI:000295799400034,
	title = {{The relative effects of particles and turbulence on acoustic scattering from deep-sea hydrothermal vent plumes}},
	journal = {JOURNAL OF THE ACOUSTICAL SOCIETY OF AMERICA},
	volume = {130},
	number = {4, 1},
	year = {2011},
	month = {oct},
	pages = {1856{\textendash}1867},
	type = {Article},
	abstract = {Acoustic methods are applied to the investigation and monitoring of a vigorous hydrothermal plume within the Main Endeavor vent field at the Endeavor segment of the Juan de Fuca Ridge. Forward propagation and scattering from suspended particulates using Rayleigh scattering theory is shown to be negligible (log-amplitude variance sigma(2)(chi) similar to 10(-7)) compared to turbulence induced by temperature fluctuations (sigma(2)(chi) similar to 0.1). The backscattering from turbulence is then quantified using the forward scattering derived turbulence level, which gives a volume backscattering strength of s(V) = 6.5 x 10(-8) m(-1). The volume backscattering cross section from particulates can range from s(V) = 3.3 x 10(-6) to 7.2 x 10(-10) m(-1) depending on the particle size. These results show that forward scatter acoustic methods in hydrothermal vent applications can be used to quantify turbulence and its effect on backscatter measurements, which can be a dominant factor depending on the particle size and its location within the plume. (C) 2011 Acoustical Society of America. {\{}[{\}}DOI: 10.1121/1.3624816]},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0001-4966},
	doi = {10.1121/1.3624816},
	author = {Xu, Guangyu and Di Iorio, Daniela}
}
@article {Gartman2011,
	title = {{Sulfide Oxidation across Diffuse Flow Zones of Hydrothermal Vents}},
	journal = {Aquatic Geochemistry},
	volume = {17},
	number = {4-5},
	year = {2011},
	month = {sep},
	pages = {583{\textendash}601},
	publisher = {SPRINGER},
	address = {NEW YORK; 233 SPRING ST, NEW YORK, NY 10013 USA},
	abstract = {The sulfide (H(2)S/HS(-)) that is emitted from hydrothermal vents begins to oxidize abiotically with oxygen upon contact with ambient bottom water, but the reaction kinetics are slow. Here, using in situ voltammetry, we report detection of the intermediate sulfur oxidation products polysulfides [S(x)(2-)] and thiosulfate [S(2)O(3)(2-)], along with contextual data on sulfide, oxygen, and temperature. At Lau Basin in 2006, thiosulfate was identified in less than one percent of approximately 10,500 scans and no polysulfides were detected. Only five percent of 11,000 voltammetric scans taken at four vent sites at Lau Basin in May 2009 show either thiosulfate or polysulfides. These in situ data indicate that abiotic sulfide oxidation does not readily occur as H(2)S contacts oxic bottom waters. Calculated abiotic potential sulfide oxidation rates are},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1380-6165},
	doi = {10.1007/s10498-011-9136-1},
	author = {Gartman, A and Yucel, M and Madison, A S and Chu, D W and Ma, S and Janzen, C P and Becker, E L and Beinart, R A and Girguis, P R and Luther, G W}
}
@article {Luther2011,
	title = {{Thermodynamics and kinetics of sulfide oxidation by oxygen: a look at inorganically controlled reactions and biologically mediated processes in the environment}},
	journal = {Frontiers in Microbiology},
	volume = {2},
	year = {2011},
	pages = {62},
	publisher = {FRONTIERS RESEARCH FOUNDATION},
	address = {LAUSANNE; PO BOX 110, LAUSANNE, 1015, SWITZERLAND},
	abstract = {The thermodynamics for the first electron transfer step for sulfide and oxygen indicates that the reaction is unfavorable as unstable superoxide and bisulfide radical ions would need to be produced. However, a two-electron transfer is favorable as stable S(0) and peroxide would be formed, but the partially filled orbitals in oxygen that accept electrons prevent rapid kinetics. Abiotic sulfide oxidation kinetics improve when reduced iron and/or manganese are oxidized by oxygen to form oxidized metals which in turn oxidize sulfide. Biological sulfur oxidation relies on enzymes that have evolved to overcome these kinetic constraints to affect rapid sulfide oxidation. Here we review the available thermodynamic and kinetic data for H2S and HS center dot as well as O-2, reactive oxygen species, nitrate, nitrite, and NOx species. We also present new kinetic data for abiotic sulfide oxidation with oxygen in trace metal clean solutions that constrain abiotic rates of sulfide oxidation in metal free solution and agree with the kinetic and thermodynamic calculations. Moreover, we present experimental data that give insight on rates of chemolithotrophic and photolithotrophic sulfide oxidation in the environment. We demonstrate that both anaerobic photolithotrophic and aerobic chemolithotrophic sulfide oxidation rates are three or more orders of magnitude higher than abiotic rates suggesting that in most environments biotic sulfide oxidation rates will far exceed abiotic rates due to the thermodynamic and kinetic constraints discussed in the first section of the paper. Such data reshape our thinking about the biotic and abiotic contributions to sulfide oxidation in the environment.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1664-302X},
	doi = {10.3389/fmicb.2011.00062},
	author = {Luther, G W and Findlay, A J and MacDonald, D J and Owings, S M and Hanson, T E and Beinart, R A and Girguis, P R}
}
@article {ISI:000208863500072,
	title = {{Thermodynamics and kinetics of sulfide oxidation by oxygen: a look at inorganically controlled reactions and biologically mediated processes in the environment}},
	journal = {FRONTIERS IN MICROBIOLOGY},
	volume = {2},
	year = {2011},
	type = {Article},
	abstract = {The thermodynamics for the first electron transfer step for sulfide and oxygen indicates that the reaction is unfavorable as unstable superoxide and bisulfide radical ions would need to be produced. However, a two-electron transfer is favorable as stable S(0) and peroxide would be formed, but the partially filled orbitals in oxygen that accept electrons prevent rapid kinetics. Abiotic sulfide oxidation kinetics improve when reduced iron and/or manganese are oxidized by oxygen to form oxidized metals which in turn oxidize sulfide. Biological sulfur oxidation relies on enzymes that have evolved to overcome these kinetic constraints to affect rapid sulfide oxidation. Here we review the available thermodynamic and kinetic data for H2S and HS center dot as well as O-2, reactive oxygen species, nitrate, nitrite, and NOx species. We also present new kinetic data for abiotic sulfide oxidation with oxygen in trace metal clean solutions that constrain abiotic rates of sulfide oxidation in metal free solution and agree with the kinetic and thermodynamic calculations. Moreover, we present experimental data that give insight on rates of chemolithotrophic and photolithotrophic sulfide oxidation in the environment. We demonstrate that both anaerobic photolithotrophic and aerobic chemolithotrophic sulfide oxidation rates are three or more orders of magnitude higher than abiotic rates suggesting that in most environments biotic sulfide oxidation rates will far exceed abiotic rates due to the thermodynamic and kinetic constraints discussed in the first section of the paper. Such data reshape our thinking about the biotic and abiotic contributions to sulfide oxidation in the environment.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1664-302X},
	doi = {10.3389/fmicb.2011.00062},
	author = {Luther III, George W and Findlay, Alyssa J and MacDonald, Daniel J and Owings, Shannon M and Hanson, Thomas E and Beinart, Roxanne A and Girguis, Peter R}
}
@article {ISI:000294119400002,
	title = {{Three new species of pennatulacean octocorals with the ability to attach to rocky substrata (Cnidaria: Anthozoa: Pennatulacea)}},
	journal = {ZOOTAXA},
	number = {3001},
	year = {2011},
	month = {aug},
	pages = {33{\textendash}48},
	type = {Article},
	abstract = {All sea pens have been thought to anchor in soft sediment using a basal, sausage-shaped, muscular peduncle. Based on underwater images and examination of specimens, we report an adaptation of the proximal portion of the peduncle for attachment to solid surfaces. We document four species with this adaptation, three new, Anthoptilum lithophilum sp. nov. (California, 669-700 m), A. gowlettholmesae sp. nov. (Tasmania, 729-1803 m), and Calibelemnon francei sp. nov. (the Bahamas, 1969 m), and one known, A. decipiens Thomson {\&} Henderson, 1906 (Sri Lanka, 925 m). The peduncle of a colony with this adaptation is greatly expanded by an outgrowth of the coenenchyme that forms a sucker-like structure, beneath which a conical mass of tough tissue surrounds the proximal end of the internal axis. We infer this structure affects suction, increasing or decreasing the strength of adhesion to the substratum, and discuss the systematics and functional morphology of this new ecological phenomenon-pennatulaceans fastened to hard substrata. We alter the definition of the genus Anthoptilum to accommodate this morphology, reporting on specimens of the type species, A. grandiflorum; compare the two sea pen families-Anthoptilidae and Scleroptilidae- and the two genera; and present a key to the known rock-inhabiting species.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1175-5326},
	author = {Williams, Gary C and Alderslade, Philip}
}
@article {Bennett2011a,
	title = {{Tracing iron-fueled microbial carbon production within the hydrothermal plume at the Loihi Seamount}},
	journal = {Geochimica et Cosmochimica Acta},
	volume = {75},
	number = {19},
	year = {2011},
	pages = {5526{\textendash}5539},
	abstract = {The Loihi hydrothermal plume provides an opportunity to investigate iron (Fe) oxidation and microbial processes in a system that is truly Fe dominated and distinct from mid-ocean ridge spreading centers. The lack of hydrogen sulfide within the Loihi hydrothermal fluids and the presence of an oxygen minimum zone at this submarine volcano{\textquoteright}s summit, results in a prolonged presence of reduced Fe within the dispersing non-buoyant plume. In this study, we have investigated the potential for microbial carbon fixation within the Loihi plume. We sampled for both particulate and dissolved organic carbon in hydrothermal fluids, microbial mats growing around vents, and the dispersing plume, and carried out stable carbon isotope analysis on the particulate fraction. The $δ$13C values of the microbial mats ranged from -23{\textperthousand} to -28{\textperthousand}, and are distinct from those of deep-ocean particulate organic carbon (POC). The mats and hydrothermal fluids were also elevated in dissolved organic carbon (DOC) compared to background seawater. Within the hydrothermal plume, DOC and POC concentrations were elevated and the isotopic composition of POC within the plume suggests mixing between background seawater POC and a 13C-depleted hydrothermal component. The combination of both DOC and POC increasing in the dispersing plume that cannot solely be the result of entrainment and DOC adsorption, provides strong evidence for in-situ microbial productivity by chemolithoautotrophs, including a likelihood for iron-oxidizing microorganisms.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0016-7037},
	doi = {10.1016/j.gca.2011.06.039},
	url = {http://dx.doi.org/10.1016/j.gca.2011.06.039},
	author = {Bennett, S A and Hansman, R L and Sessions, A L and Nakamura, K and Edwards, K J}
}
@article {Seyfried2011,
	title = {{Vent fluid chemistry of the Rainbow Hydrothermal System (36 degrees N, MAR): Phase equilibria and in-situ pH controls on subseafloor alteration processes}},
	journal = {Geochimica et Cosmochimica Acta},
	volume = {75},
	number = {6},
	year = {2011},
	pages = {1574{\textendash}1593},
	abstract = {The Rainbow hydrothermal field is located at 36{\textdegree}13.8'N{\textendash}33{\textdegree}54.15'W at 2300~m depth on the western flank of a non-volcanic ridge between the South AMAR and AMAR segments of the Mid-Atlantic Ridge. The hydrothermal field consists of 10{\textendash}15 active chimneys that emit high-temperature (\~{}365~{\textdegree}C) fluid. In July 2008, vent fluids were sampled during cruise KNOX18RR, providing a rich dataset that extends in time information on subseafloor chemical and physical processes controlling vent fluid chemistry at Rainbow. Data suggest that the Mg concentration of the hydrothermal end-member is not zero, but rather 1.5{\textendash}2~mmol/kg. This surprising result may be caused by a combination of factors including moderately low dissolved silica, low pH, and elevated chloride of the hydrothermal fluid. Combining end-member Mg data with analogous data for dissolved Fe, Si, Al, Ca, and H2, permits calculation of mineral saturation states for minerals thought appropriate for ultramafic-hosted hydrothermal systems at temperatures and pressures in keeping with constraints imposed by field observations. These data indicate that chlorite solid solution, talc, and magnetite achieve saturation in Rainbow vent fluid at a similar pH(T,P) (400~{\textdegree}C, 500~bar) of approximately 4.95, while higher pH values are indicated for serpentine, suggesting that serpentine may not coexist with the former assemblage at depth at Rainbow. The high Fe/Mg ratio of the Rainbow vent fluid notwithstanding, the mole fraction of clinochlore and chamosite components of chlorite solid solution at depth are predicted to be 0.78 and 0.22, respectively. In situ pH measurements made at Rainbow vents are in good agreement with pH(T,P) values estimated from mineral solubility calculations, when the in situ pH data are adjusted for temperature and pressure. Calculations further indicate that pH(T,P) and dissolved H2 are extremely sensitive to changes in dissolved silica owing to constraints imposed by chlorite solid solution-fluid equilibria. Indeed, the predicted correlation between dissolved silica and H2 defines a trend that is in good agreement with vent fluid data from Rainbow and other high-temperature ultramafic-hosted hydrothermal systems. We speculate that the moderate concentrations of dissolved silica in vent fluids from these systems result from hydrothermal alteration of plagioclase and olivine in the form of subsurface gabbroic intrusions, which, in turn are variably replaced by chlorite~+~magnetite~+~talc~{\textpm}~tremolite, with important implications for pH lowering, dissolved sulfide concentrations, and metal mobility.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0016-7037},
	doi = {10.1016/j.gca.2011.01.001},
	url = {http://dx.doi.org/10.1016/j.gca.2011.01.001},
	author = {Seyfried, W E and Pester, N J and Ding, K and Rough, M}
}
@article {Wanless2011,
	title = {{Volatile abundances and oxygen isotopes in basaltic to dacitic lavas on mid-ocean ridges: The role of assimilation at spreading centers}},
	journal = {Chemical Geology},
	volume = {287},
	number = {1-2},
	year = {2011},
	pages = {54{\textendash}65},
	abstract = {Most geochemical variability in MOR basalts is consistent with low- to moderate-pressure fractional crystallization of various mantle-derived parental melts. However, our geochemical data from MOR high-silica glasses, including new volatile and oxygen isotope data, suggest that assimilation of altered crustal material plays a significant role in the petrogenesis of dacites and may be important in the formation of basaltic lavas at MOR in general. MOR high-silica andesites and dacites from diverse areas show remarkably similar major element trends, incompatible trace element enrichments, and isotopic signatures suggesting similar processes control their chemistry. In particular, very high Cl and elevated H2O concentrations and relatively light oxygen isotope ratios ({\~{}}~5.8{\textperthousand} vs. expected values of {\~{}}~6.8{\textperthousand}) in fresh dacite glasses can be explained by contamination of magmas from a component of ocean crust altered by hydrothermal fluids. Crystallization of silicate phases and Fe-oxides causes an increase in $δ$18O in residual magma, but assimilation of material initially altered at high temperatures results in lower $δ$18O values. The observed geochemical signatures can be explained by extreme fractional crystallization of a MOR basalt parent combined with partial melting and assimilation (AFC) of amphibole-bearing altered oceanic crust. The MOR dacitic lavas do not appear to be simply the extrusive equivalent of oceanic plagiogranites. The combination of partial melting and assimilation produces a distinct geochemical signature that includes higher incompatible trace element abundances and distinct trace element ratios relative to those observed in plagiogranites.},
	keywords = {ROV DSL-120 (Remotely Operated Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0009-2541},
	doi = {10.1016/j.chemgeo.2011.05.017},
	url = {http://dx.doi.org/10.1016/j.chemgeo.2011.05.017},
	author = {Wanless, V D and Perfit, M R and Ridley, W I and Wallace, P J and Grimes, C B and Klein, E M}
}
@article {Clague2011,
	title = {{Volcanic morphology of West Mata Volcano, NE Lau Basin, based on high-resolution bathymetry and depth changes}},
	journal = {Geochemistry, Geophysics, Geosystems},
	volume = {12},
	year = {2011},
	month = {nov},
	pages = {QOAF03{\textendash}QOAF03},
	publisher = {AMER GEOPHYSICAL UNION},
	address = {WASHINGTON; 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA},
	abstract = {High-resolution (1.5 m) mapping from the autonomous underwater vehicle (AUV) D. Allan B. of West Mata Volcano in the northern Lau Basin is used to identify the processes that construct and modify the volcano. The surface consists largely of volcaniclastic debris that forms smooth slopes to the NW and SE, with smaller lava flows forming gently sloping plateaus concentrated along the ENE and WSW rift zones, and more elongate flows radiating from the summit. Two active volcanic vents, Prometheus and Hades, are located \~{}50 and \~{}150 m WSW of the 1159 m summit, respectively, and are slightly NW of the ridgeline so the most abundant clastic deposits are emplaced on the NW flank. This eruptive activity and the location of vents appears to have been persistent for more than a decade, based on comparison of ship-based bathymetric surveys in 1996 and 2008{\textendash}2010, which show positive depth changes up to 96 m on the summit and north flank of the volcano. The widespread distribution of clastic deposits downslope from the rift zones, as well as from the current vents, suggests that pyroclastic activity occurs at least as deep as 2200 m. The similar morphology of additional nearby volcanoes suggests that they too have abundant pyroclastic deposits.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1525-2027},
	doi = {10.1029/2011GC003791},
	author = {Clague, D A and Paduan, J B and Caress, D W and Thomas, Hans and Chadwick, W W and Merle, S G}
}
@article {Roman2010,
	title = {{Autonomous underwater vehicles as tools for deep-submergence archaeology}},
	journal = {Proceedings of the Institution of Mechanical Engineers, Part M: Journal of Engineering for the Maritime Environment},
	volume = {224},
	number = {4},
	year = {2010},
	pages = {327{\textendash}340},
	abstract = {Marine archaeology beyond the capabilities of scuba divers is a technologically enabled field. The tool suite includes ship-based systems such as towed side-scan sonars and remotely operated vehicles, and more recently free-swimming autonomous underwater vehicles (AUVs). Each of these platforms has various imaging and mapping capabilities appropriate for specific scales and tasks. Broadly speaking, AUVs are becoming effective tools for locating, identifying, and surveying archaeological sites. This paper discusses the role of AUVs in this suite of tools, outlines some specific design criteria necessary to maximize their utility in the field, and presents directions for future developments. Results are presented for a recent joint AUV{\textemdash}towed system survey and a demonstration of current mine-hunting technologies applied to archaeology.},
	keywords = {AUV ABE (Autonomous Benthic Explorer), ROV Jason (Remotely Operated Vehicle)},
	issn = {1475-0902},
	url = {http://pim.sagepub.com/content/224/4/327.abstract},
	author = {Roman, C and Mather, R}
}
@article {Joye2010,
	title = {{Biogeochemical signatures and microbial activity of different cold-seep habitats along the Gulf of Mexico deep slope}},
	journal = {Deep-Sea Research. Part II: Topical Studies in Oceanography},
	volume = {57},
	number = {21-23},
	year = {2010},
	pages = {1990{\textendash}2001},
	abstract = {Microorganisms and the processes they mediate serve as the metabolic foundation of cold seeps. We characterized a suite of biogeochemical constituents and quantified rates of two key microbial processes, Sulfate Reduction (SR) and Anaerobic Oxidation of Methane (AOM), to assess variability between habitats at water depths exceeding 1000 m in the northern Gulf of Mexico. Rates of SR were highest in sediments beneath microbial mats, lower in brine-influenced and oil-influenced sediments, and lowest in animal habitats. Sediments collected near tubeworms had the highest SR rates for animal habitats. Rates of AOM generally were low, but higher rates were associated with brine-influenced, oil-influenced, tubeworm- and urchin-inhabited sediments. Rates of both SR and AOM were orders of magnitude lower at deep-slope sites compared to upper-slope sites examined previously. As observed at upper-slope sites, SR and AOM rates were often loosely coupled. At one site, AOM rates exceeded SR rates, suggesting that an alternate electron acceptor for AOM is possible. Extremely depleted $δ$13C values in methane illustrated the broad significance of biogenic methane production at deep-slope sites. Brine-influenced habitats were characterized by extremely high concentrations of ammonium and dissolved organic carbon, serving as important focused sources of these chemicals to adjacent environments.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0645},
	doi = {10.1016/j.dsr2.2010.06.001},
	url = {http://dx.doi.org/10.1016/j.dsr2.2010.06.001},
	author = {Joye, S B and Bowles, M W and Samarkin, V A and Hunter, K S and Niemann, H}
}
@article {Podowski2010,
	title = {{Biotic and abiotic factors affecting distributions of megafauna in diffuse flow on andesite and basalt along the Eastern Lau Spreading Center, Tonga}},
	journal = {Marine Ecology Progress Series},
	volume = {418},
	year = {2010},
	pages = {25{\textendash}45},
	abstract = {Imagery and environmental data from 7 diffuse flow hydrothermal vent sites along the Eastern Lau Spreading Center (ELSC) are used to constrain the effects of lava type, temperature, chemistry, and biological interactions on faunal distributions. Of the species with chemoautotrophic endosymbionts, the snail Alviniconcha spp. occupies habitats with the greatest exposure to vent fluids. Temperatures exceeding 45{\textdegree}C define its upper limit of exposure to vent flow, and minimum sulfide requirements constrain its lower limits. The mussel Bathymodiolus brevior experiences the least exposure to vent flow; temperatures of about 20{\textdegree}C determine its upper limit, while its lower limit is defined by its minimum sulfide requirements. The snail Ifremeria nautilei inhabits areas with intermediate exposure to vent fluids and biological interactions are likely the most important factor shaping this snail{\textquoteright}s realized niche. Microhabitats of non-symbiont-containing fauna were defined in terms of symbiont-containing faunal distributions. The crab Austinograea spp. occupies areas with the greatest exposure to vent flow; shrimp, the snail Eosipho desbruyeresi, and anemones inhabit intermediate zones of vent flow; and the squat lobster Munidopsis lauensis dominates the periphery of diffuse flow areas, with little exposure to vent fluids. The physical structure of different lava types along the ELSC differentially affects the diffusion of vent fluids, which has a variety of implications for fauna, particularly distributions of zoanthids, anemones, and mixed communities of I. nautilei and B. brevior.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.3354/meps08797},
	author = {Podowski, E L and Ma, S and Luther, G W and Wardrop, D and Fisher, C R}
}
@article {Roberts2010a,
	title = {{Cold-seep carbonates of the middle and lower continental slope, northern Gulf of Mexico}},
	journal = {Deep-Sea Research. Part II: Topical Studies in Oceanography},
	volume = {57},
	number = {21-23},
	year = {2010},
	pages = {2040{\textendash}2054},
	abstract = {Authigenic carbonates from cold seeps on the middle and lower continental slope of the northern Gulf of Mexico (GOM) exhibit a wide range of mineralogical and stable isotopic compositions. These carbonates consist of concretions and nodules in surface sediments, hardgrounds of crusts and isolated slabs, and mounded buildups of blocks and slabs of up to over 10 meters in relief above the surrounding seafloor. Mineralogically, the carbonates are dominated by high-Mg calcite (HMC) and aragonite. However, low levels ({\textless}5 wt{\%}) of dolomite are present in most samples. Petrographically, Mg-calcite peloidal matrix and acicular to botryoidal aragonitic void-filling cements are the most frequent associations. The carbon isotopic compositions of the carbonates range from -60.8 to 14.0{\textperthousand} PDB, indicating complex carbon sources that include 13C-depleted biogenic and thermogenic methane, biodegraded crude oil, seawater CO2, and 13C-enriched residual CO2 from methanogenesis. A similarly large variability in $δ$18O values (2.5 to 6.7{\textperthousand} PDB) demonstrates the geochemical complexity of the slope, with some samples pointing toward an 18O-enriched oxygen source that is possibly related to advection of 18O-enriched formation water and/or to the decomposition of gas hydrate. A considerable range of mineralogical and isotopic variations in cold-seep carbonate composition was noted even within individual study sites. However, common trends occur across multiple geographic areas. This situation suggests that local controls on fluid and gas flux, types of seep hydrocarbons, the presence or absence of gas hydrate in the near-surface sediment, and chemosynthetic communities, as well as the temporal evolution of the local hydrocarbon reservoir, all may play a part in determining carbonate mineralogy and isotope geochemistry. The carbon isotope data clearly indicate that between-site variation is greater than within-site variation. Seep carbonates formed on the middle and lower continental slope of the GOM do not appear to be substantially different from those found on the upper slope ({\textless}1000-m water depth). The highly variable fluids and gases that leave their geochemical imprints on seep carbonate of the middle and lower continental slope are similar to their outer shelf and upper slope counterparts.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0645},
	doi = {10.1016/j.dsr2.2010.09.003},
	url = {http://dx.doi.org/10.1016/j.dsr2.2010.09.003},
	author = {Roberts, H H and Feng, D and Joye, S B}
}
@article {Bernhard2010,
	title = {{Combined carbonate carbon isotopic and cellular ultrastructural studies of individual benthic foraminifera : 2. Toward an understanding of apparent disequilibrium in hydrocarbon seeps}},
	journal = {Paleoceanography},
	volume = {25},
	year = {2010},
	pages = {PA4206},
	abstract = {Numerous previous studies show disequilibrium between stable carbon isotope ratios of foraminiferal calcite and pore water dissolved inorganic carbon in hydrocarbon seeps, calling into question the utility of this widely used paleoceanographic tracer as a proxy. We use a recently developed method to compare stable carbon isotope ratios of foraminiferal carbonate with cell ultrastructural observations from individual benthic foraminifera from seep (under chemosynthetic bivalves) and nonseep habitats in Monterey Bay, California, to better understand control(s) of benthic foraminiferal carbon isotope ratios. Two attributes previously proposed to cause the isotopic offsets are diet and symbionts. Ultrastructural analysis shows that positive staining with Rose Bengal indicates presence of foraminiferal cytoplasm, bacterial biomass, or a combination of both and, thus, is not an unequivocal indicator of viability. We also show for the first time that some living seep foraminifera have endobionts. Results from our unique, yet limited, data set are consistent with suggestions that, in our sites, several foraminiferal species collected from seep clam beds may not survive there, diet and symbiont presence do not appear to be major contributors to disequilibrium, little calcification of seep-tolerant foraminiferal species occurs while seep conditions prevail, and microscale variability in habitats could influence $δ$13C of benthic foraminiferal carbonate. Results further suggest that our knowledge of benthic foraminiferal ecology and biomineralization, especially in extreme habitats such as seeps, must be bolstered before we fully understand the fidelity of paleoenvironmental records derived from benthic foraminiferal test $δ$13C data.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0883-8305},
	doi = {10.1029/2010PA001930},
	url = {http://dx.doi.org/10.1029/2010PA001930},
	author = {Bernhard, J M and Martin, J B and Rathburn, A E}
}
@article {Martin2010,
	title = {{Combined carbonate carbon isotopic and cellular ultrastructural studies of individual benthic foraminifera: Method description}},
	journal = {Paleoceanography},
	volume = {25},
	year = {2010},
	pages = {PA2211},
	abstract = {Carbon isotopes of foraminiferal tests provide a widely used proxy for past oceanographic environmental conditions. This proxy can be calibrated using live specimens, which are reliably identified with observations of cell ultrastructure. Observations of ultrastructures can also be used for studies of biological characteristics such as diet and presence of symbionts. Combining biological and isotopic studies on individual foraminifera could provide novel information, but standard isotopic methods destroy ultrastructures by desiccating specimens and observations of ultrastructure require removal of carbonate tests, preventing isotope measurements. The approach described here preserves cellular ultrastructure during isotopic analyses by keeping the foraminifera in an aqueous buffer (Phosphate Buffered Saline (PBS)). The technique was developed and standardized with 36 aliquots of NBS-19 standard of similar weight to foraminiferal tests (5 to 123 $μ$g). Standard errors ranged from {\textpm} 0.06 to {\textpm} 0.85{\textperthousand} and were caused by CO2 contaminants dissolved in the PBS. The technique was used to measure $δ$13C values of 96 foraminifera, 10 of which do not precipitate carbonate tests. Calcareous foraminiferal tests had corrected carbon isotope ratios of -8.5 to +3.2{\textperthousand}. This new technique allows comparisons of isotopic compositions of tests made by foraminifera known to be alive at the time of collection with their biological characteristics such as prey composition and presence or absence of putative symbionts. The approach may be applied to additional biomineralizing organisms such as planktonic foraminifera, pteropods, corals, and coccolithophores to elucidate certain biological controls on their paleoceanographic proxy signatures.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0883-8305},
	doi = {10.1029/2009PA001846},
	url = {http://dx.doi.org/10.1029/2009PA001846},
	author = {Martin, J B and Bernhard, J M and Curtis, J and Rathburn, A E}
}
@article {Lessard-Pilon2010,
	title = {{Community composition and temporal change at deep Gulf of Mexico cold seeps}},
	journal = {Deep-Sea Research. Part II: Topical Studies in Oceanography},
	volume = {57},
	number = {21-23},
	year = {2010},
	pages = {1891{\textendash}1903},
	abstract = {Specialized cold-seep communities have been known to exist in the Gulf of Mexico (GOM) since the mid-1980s, but only recently has extensive research been carried out on sites at depths {\textgreater}1000 m. This study uses a combination of imagery and analyses within a Geographic Information Systems (GIS) framework to examine the composition of mussel and tubeworm communities at depths between 2200 and 2800 m in the Gulf of Mexico, spatial relations among the fauna, and changes in these communities over time. Photomosaics at three discrete seep communities were obtained in 2006 and a video mosaic of another community was obtained in 1992. Each of these communities was re-imaged in 2007. In addition, quantitative physical collections were made within two of the photomosaic sites and used to confirm the identification of megafauna, quantify the occurrence of smaller and cryptic macrofauna, and allow first-order calculations of biomass within the sites. Substrate type had a significant effect on community composition. Significant associations were identified between live mussels with anemones, shrimp, and sea cucumbers, and between tubeworm aggregations and Munidopsis sp. crabs and encrusting fauna, indicating differences in the composition of megafauna associated with adjacent mussel and tubeworm aggregations. Little change was seen in the total area colonized by foundation fauna (tubeworms and mussels) between years at any site. However, significant changes occurred in the positions of mussels, even over periods of a single year, at all sites, and evidence for the establishment of new tubeworm aggregations between 1992 and 2007 was noted at one site. These photomosaics provide data suggesting that environmental conditions can change over small spatial and temporal scales and mussels move in response to these changes. The successional trends are examined and compared to the patterns that have been documented in shallow ({\textless}1000-m depth) Gulf of Mexico seep communities.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0645},
	doi = {10.1016/j.dsr2.2010.05.012},
	url = {http://dx.doi.org/10.1016/j.dsr2.2010.05.012},
	author = {Lessard-Pilon, S and Porter, M D and Cordes, E E and MacDonald, I R and Fisher, C R}
}
@article {MacDonald2010,
	title = {{Community structure comparisons of lower slope hydrocarbon seeps, northern Gulf of Mexico}},
	journal = {Deep-Sea Research. Part II: Topical Studies in Oceanography},
	volume = {57},
	number = {21-23},
	year = {2010},
	pages = {1904{\textendash}1915},
	abstract = {Photographic sampling collected from ROV JASON III on randomized transects (generally 10 per site) was used to compare seven suspected hydrocarbon seeps on the lower continental slope at depths of 956 to 2330 m. The study design focused on areas containing high-amplitude reflectance patterns in seismic returns from the bottom. Animal samples nested in these areas and high-resolution photographic mosaics permitted fine-scale description of the community components and their associations with each other. The transect results showed three types of chemosynthetic community: carbonate prone, brine pool prone, and low-density. These communities are characterized by, respectively, abundant carbonate rubble and tubeworm clusters, bacterial mats and brine pools, and relatively sparse chemosynthetic fauna that tended to be overlooked by the photographic survey plan. These differences were not clearly related to depth or longitude because the sites are at the opposite eastern and western extents of the project region. Photographic survey is a statistically reliable method for detecting the carbonate rubble and bacterial mat types of community, but may be unreliable for sparse or clustered fauna such as seep mussels. Photographic survey results provided relatively low taxonomic resolution, but were sufficient to distinguish statistical differences in the abundance of characteristic faunal groups, such as the Echinoidea and Gorgonacea, associated with hydrocarbon seeps. Initial studies of statistical power indicate that future photographic surveys of suspected seep communities, if conducted with an equivalent level of effort, might detect the presence of more abundant habitat, including carbonate rubble, bacterial mats, and tubeworms, but could miss less abundant indicators.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0645},
	doi = {10.1016/j.dsr2.2010.09.002},
	url = {http://dx.doi.org/10.1016/j.dsr2.2010.09.002},
	author = {MacDonald, I R and Smith, M and Huffer, F W}
}
@article {Roberts2010,
	title = {{Dive site geology: DSV ALVIN (2006) and ROV JASON II (2007) dives to the middle-lower continental slope, northern Gulf of Mexico}},
	journal = {Deep-Sea Research. Part II: Topical Studies in Oceanography},
	volume = {57},
	number = {21-23},
	year = {2010},
	pages = {1837{\textendash}1858},
	abstract = {Use of DSV ALVIN (2006) and ROV JASON II (2007) provided access to never observed or sampled sites of fluid-gas expulsion from the little-studied middle and lower continental slope of the northern Gulf of Mexico (below water depths of 1000 m). Dives were focused on 15 locations selected by 3-D seismic surface attributes and shallow subsurface geologic analyses. The linkage between highly positive seafloor reflectivity and hard bottoms proved to be an efficient indicator of potential sites of interest. Through observation and sampling of reflective sites, starting in the mid-1980s, it has become apparent that most hard bottoms on the northern Gulf{\textquoteright}s continental slope are created by the precipitation of authigenic carbonates at hydrocarbon seep sites. Access to the Bureau of Ocean Energy Management, Regulation and Enforcement{\textquoteright}s extraordinary archive of slope-wide 3-D seismic data made efficient site selection possible. From thousands of sites that display the characteristics of fluid-gas expulsion, 15 were observed and sampled during the 2006 and 2007 cruises. Water depths in which these 15 sites were located ranged from \~{}2750 to \~{}970 m. All sites exhibited evidence of hydrocarbon seepage or more rapid venting. Chemosynthetic organisms, authigenic carbonates, barite, gas hydrates, highly anoxic surface sediments, brine pools, and hydrocarbon-laced brine flows were identified and sampled. High-resolution acoustic Autonomous Underwater Vehicle (AUV) data, including multibeam bathymetry, side-scan sonar swaths, and chirp sonar subbottom profiles, were collected at four locations (AC601, WR269, GC852, and AT340). Data sets from the 2006 and 2007 dives resulted in a greatly improved understanding of both cross-slope and along-slope variability in the characteristics of fluid-gas expulsion sites and associated habitats. Our studies confirmed the importance of fluid-gas expulsion processes for sustaining chemosynthetic communities and impacting seabed geology on the middle and lower continental slope of the northern Gulf of Mexico.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0645},
	doi = {10.1016/j.dsr2.2010.09.001},
	url = {http://dx.doi.org/10.1016/j.dsr2.2010.09.001},
	author = {Roberts, H H and Shedd, W and Hunt, J}
}
@article {Levin2010,
	title = {{Diversity of bathyal macrofauna on the northeastern Pacific margin: the influence of methane seeps and oxygen minimum zones}},
	journal = {Marine Ecology},
	volume = {31},
	number = {1},
	year = {2010},
	pages = {94{\textendash}110},
	publisher = {Blackwell Publishing Ltd},
	abstract = {The upper continental slope in the northeastern Pacific Ocean is intercepted by a deep oxygen minimum zone (OMZ; 650{\textendash}1100 m) and punctuated by conduits of methane seepage. We examined the effects of these two dominant sources of heterogeneity on the density, composition and diversity of heterotrophic macrofauna off Hydrate Ridge, Oregon (OR; 800 m water depth), where the seeps co-occur within an OMZ, and off the Eel River, Northern California (CA; 500 m), where seeps are overlain by better oxygenated waters. We hypothesized that seeps (containing clam beds and microbial mats) should contribute a suite of distinct species to the regional margin species pool but that OMZ-associated hypoxia would dampen seep-related heterogeneity. Macrofaunal densities were highest (23,000{\textendash}33,510 ind{\textperiodcentered}m-2) in the CA seep sediments and in the OR near-seep samples, intermediate in the OR seep, CA near seep and CA and OR 500-m margin sediments (10,054{\textendash}19,777 ind{\textperiodcentered}m-2), and lowest in the CA and OR OMZ habitats at 800 m (4269{\textendash}7847 ind{\textperiodcentered}m-2). Annelids constituted over 50{\%} of the taxa in all but the CA clam bed and OR microbial mat sediments, where mollusks were abundant. Approximately 50{\%} of seep species appeared to be habitat endemic; species present in microbial mats largely formed a subset of those present in the clam beds. Dorvilleid and ampharetid polychaetes were dominant in the seep sediments; non-seep margin sediments at 500 and 800 m were populated heavily by branchiate polychaetes including cossurids and paraonids. Alpha diversity (Es[20] calculated per core) was lowest and rank 1 dominance was highest in the CA and OR microbial mat habitats. Pooled analyses of Es[100] revealed highest species richness in the CA clam bed and near-seep habitats (30.3 and 29.6, respectively), and lowest species richness in the OR microbial mat and near-seep habitats (16.5 and 17.9, respectively). Non-seep sediments (500 and 800 m) off both CA and OR were more homogeneous (55{\textendash}57{\%} within-habitat similarity) than clam bed and microbial mat sediments (only 32{\textendash}37{\%} within-habitat similarity). CA sediment macrofauna generally exhibit higher alpha diversity, and as habitats are combined, a higher rate of increase in the slope of the species accumulation curves than do OR margin macrofauna. Methane seeps in the NE Pacific introduce significant heterogeneity that increases margin biodiversity at multiple spatial scales. However, our hypothesis that the OMZ would lessen the seep contributions to diversity was not supported. The better oxygenated CA seeps at 500 m shared more of the background margin fauna (at 500 m) than did the OR seeps at 800 m (with OMZ fauna at 800 m). Geographical differences in the fluxes of methane-rich fluids and the increased reliance on chemosynthetic food sources with increased depth could explain these results.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {1439-0485},
	doi = {10.1111/j.1439-0485.2009.00335.x},
	url = {http://dx.doi.org/10.1111/j.1439-0485.2009.00335.x},
	author = {Levin, L A and Mendoza, G F and Gonzalez, J P and Thurber, A R and Cordes, E E}
}
@article {Kellogg2010,
	title = {{Enumeration of viruses and prokaryotes in deep-sea sediments and cold seeps of the Gulf of Mexico}},
	journal = {Deep-Sea Research. Part II: Topical Studies in Oceanography},
	volume = {57},
	number = {21-23},
	year = {2010},
	pages = {2002{\textendash}2007},
	abstract = {Little is known about the distribution and abundance of viruses in deep-sea cold-seep environments. Like hydrothermal vents, seeps support communities of macrofauna that are sustained by chemosynthetic bacteria. Sediments close to these communities are hypothesized to be more microbiologically active and therefore to host higher numbers of viruses than non-seep areas. Push cores were taken at five types of Gulf of Mexico habitats at water depths below 1000 m using a remotely operated vehicle (ROV). The habitats included non-seep reference sediment, brine seeps, a microbial mat, an urchin field, and a pogonophoran worm community. Samples were processed immediately for enumeration of viruses and prokaryotes without the addition of a preservative. Prokaryote counts were an order of magnitude lower in sediments directly in contact with macrofauna (urchins, pogonophorans) compared to all other samples (107 vs. 108 cells g-1 dry weight) and were highest in areas of elevated salinity (brine seeps). Viral-Like Particle (VLP) counts were lowest in the reference sediments and pogonophoran cores (108 VLP g-1 dry wt), higher in brine seeps (109 VLP g-1 dry wt), and highest in the microbial mats (1010 VLP g-1 dry wt). Virus-prokaryote ratios (VPR) ranged from {\textless}5 in the reference sediment to {\textgreater}30 in the microbial mats and {\textgreater}60 in the urchin field. VLP counts and VPR were all significantly greater than those reported from sediments in the deep Mediterranean Sea and in most cases were higher than recent data from a cold-seep site near Japan. The high VPR suggest that greater microbial activity in or near cold-seep environments results in greater viral production and therefore higher numbers of viruses.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0645},
	doi = {10.1016/j.dsr2.2010.05.006},
	url = {http://dx.doi.org/10.1016/j.dsr2.2010.05.006},
	author = {Kellogg, C A}
}
@article {Bright2010,
	title = {{Epizooic metazoan meiobenthos associated with tubeworm and mussel aggregations from cold seeps of the northern Gulf of Mexico}},
	journal = {Deep-Sea Research. Part II: Topical Studies in Oceanography},
	volume = {57},
	number = {21-23},
	year = {2010},
	pages = {1982{\textendash}1989},
	abstract = {The abundance and higher taxonomic composition of epizooic metazoan meiobenthic communities associated with mussel and tubeworm aggregations of hydrocarbon seeps at Green Canyon, Atwater Valley, and Alaminos Canyon in depths between 1400 and 2800 m were studied and compared to the infaunal community of non-seep sediments nearby. Epizooic meiofaunal abundances of associated meiobenthos living in tubeworm bushes and mussel beds at seeps were extremely low (usually {\textless}100 ind. 10 cm-2), similar to epizooic meiofauna at deep-sea hydrothermal vents, and the communities were composed primarily of nematodes, copepods, ostracods, and halacarids. In contrast, epizooic meiobenthic abundance is lower than previous studies have reported for infauna from seep sediments. Interestingly, non-seep sediments contained higher abundances and higher taxonomic diversity than epizooic seep communities, although in situ primary production is restricted to seeps.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0645},
	doi = {10.1016/j.dsr2.2010.05.003},
	url = {http://dx.doi.org/10.1016/j.dsr2.2010.05.003},
	author = {Bright, M and Plum, C and Riavitz, L A and Arbizu, P M and Cordes, E E and Gollner, S}
}
@article {Tunnicliffe2010,
	title = {{Flatfish at seamount hydrothermal vents show strong genetic divergence between volcanic arcs}},
	journal = {Marine Ecology-an Evolutionary Perspective},
	volume = {31},
	year = {2010},
	month = {sep},
	pages = {158{\textendash}167},
	abstract = {Hydrothermal vents near the summits of seamounts in Western Pacific volcanic arcs foster dense populations of flatfishes, a group otherwise unknown at vents. We examined genetic divergence among populations of a symphurine tonguefish described as Symphurus thermophilus Munroe {\&} Hashimoto from sites up to 6000 km apart in the Western Pacific to explore connectivity patterns among seamounts. Average genetic divergence between individuals from the Mariana Arc and the Tonga-Kermadec Arcs was 14.2{\%} (COI) and 9.0{\%} (16S), whereas within-arc divergences were {\textless}0.3{\%}. We found that the Tonga-Kermadec individuals represent a cryptic species, Symphurus sp. A, displaying similar phenotypic features and behaviour to the Mariana Arc S. thermophilus. We also sequenced another distinctive symphurine species; Symphurus sp. B. Collections and image records from three expeditions to the Tonga and Kermadec arcs revealed characteristics of the distribution, dispersion, behaviour and morphology of these flatfish species. These two new Symphurus species inhabit vents where native sulphur occurs in excess and depths are {\textless}600 m. Substratum range of Symphurus sp. A was wide, including mussel beds, rock surfaces and sediments in densities that may exceed 100 m-2. Reproductive females were present. The complex and diverse nature of volcanic settings of hydrothermalism introduces a wide variety of habitat conditions that likely augments diversity of faunas on seamounts in distinct biogeographic provinces.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0173-9565},
	doi = {10.1111/j.1439-0485.2010.00370.x},
	author = {Tunnicliffe, V and Koop, B F and Tyler, J and So, Stacy}
}
@article {ISI:000280633000010,
	title = {{Functional genes as markers for sulfur cycling and CO2 fixation in microbial communities of hydrothermal vents of the Logatchev field}},
	journal = {FEMS MICROBIOLOGY ECOLOGY},
	volume = {73},
	number = {3},
	year = {2010},
	month = {sep},
	pages = {526{\textendash}537},
	type = {Article},
	abstract = {Life at deep-sea hydrothermal vents depends on chemolithoautotrophic microorganisms as primary producers mediating the transfer of energy from hydrothermal fluids to higher trophic levels. A comprehensive molecular survey was performed with microbial communities in a mussel patch at the Irina II site of the Logatchev hydrothermal field by combining the analysis of 16S rRNA gene sequences with studies of functional key genes involved in biochemical pathways of sulfur oxidation-reduction (soxB, aprA) and autotrophic carbon fixation (aclB, cbbM, cbbL). Most significantly, major groups of chemoautotrophic sulfur oxidizers in the diffuse fluids differed in their biosynthetic pathways of both carbon fixation and sulfur oxidation. One important component of the community, the Epsilonproteobacteria, has the potential to grow chemoautotrophically by means of the reductive tricarboxylic acid cycle and to gain energy through the oxidation of reduced sulfur compounds using the Sox pathway. The majority of soxB and all retrieved aclB gene sequences were assigned to this group. Another important group in this habitat, the Gammaproteobacteria, may use the adenosine 5{\textquoteright}-phosphosulfate pathway and the Calvin-Benson-Bassham cycle, deduced from the presence of aprA and cbbM genes. Hence, two important groups of primary producers at the investigated site might use different pathways for sulfur oxidation and carbon fixation.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0168-6496},
	doi = {10.1111/j.1574-6941.2010.00919.x},
	author = {Huegler, Michael and Gaertner, Andrea and Imhoff, Johannes F}
}
@article {ISI:000284431900008,
	title = {{Genetic and anatomic relationships among three morphotypes of the echinoid Echinocrepis rostrata}},
	journal = {INVERTEBRATE BIOLOGY},
	volume = {129},
	number = {4},
	year = {2010},
	pages = {368{\textendash}375},
	type = {Article},
	abstract = {Three morphotypes-purple, tan, and white-of an irregular echinoid of the genus Echinocrepis are commonly observed in the deep North Pacific Ocean. Mitochondrial (mt) 16S DNA and cytochrome c oxidase subunit I (COI) DNA sequences were amplified from gonadal tissue samples from specimens of each morphotype. These portions of the mitochondrial genome are commonly used in species barcoding, and their similarities were compared and placed in 95{\%} connection limit parsimony networks. All three morphotypes have similar 16S mtDNA and COI mtDNA sequences, and are thus likely representatives of the same species, Echinocrepis rostrata. Specimens of the white morphotype were smaller than the specimens of the other two morphotypes, had less gonad tissue, and had a different body shape, suggesting that it represents the juvenile form of E. rostrata. Resolving the three morphotypes into one species simplifies the identification of E. rostrata from photographs and leads to a greater understanding of the life history and reproductive cycle of a species vital to deep-sea bioturbation and carbon sequestration.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {1077-8306},
	doi = {10.1111/j.1744-7410.2010.00205.x},
	author = {Vardaro, Michael F}
}
@article {Johnson2010,
	title = {{Hydrothermal circulation within the Endeavour Segment, Juan de Fuca Ridge}},
	journal = {Geochemistry, Geophysics, Geosystems},
	volume = {11},
	year = {2010},
	pages = {Q05002},
	abstract = {Areas of the seafloor at mid-ocean ridges where hydrothermal vents discharge are easily recognized by the dramatic biological, physical, and chemical processes that characterize such sites. Locations where seawater flows into the seafloor to recharge hydrothermal cells within the crustal reservoir are by contrast almost invisible but can be indirectly identified by a systematic grid of conductive heat flow measurements. An array of conductive heat flow stations in the Endeavour axial valley of the Juan de Fuca Ridge has identified recharge zones that appear to represent a nested system of fluid circulation paths. At the scale of an axial rift valley, conductive heat flow data indicate a general cross-valley fluid flow, where seawater enters the shallow subsurface crustal reservoir at the eastern wall of the Endeavour axial valley and undergoes a kilometer of horizontal transit beneath the valley floor, finally exiting as warm hydrothermal fluid discharge on the western valley bounding wall. Recharge zones also have been identified as located within an annular ring of very cold seafloor around the large Main Endeavour Hydrothermal Field, with seawater inflow occurring within faults that surround the fluid discharge sites. These conductive heat flow data are consistent with previous models where high-temperature fluid circulation cells beneath large hydrothermal vent fields may be composed of narrow vertical cylinders. Subsurface fluid circulation on the Endeavour Segment occurs at various crustal depths in three distinct modes: (1) general east to west flow across the entire valley floor, (2) in narrow cylinders that penetrate deeply to high-temperature heat sources, and (3) supplying low-temperature diffuse vents where seawater is entrained into the shallow uppermost crust by the adjacent high-temperature cylindrical systems. The systematic array of conductive heat flow measurements over the axial valley floor averaged \~{}150 mW/m2, suggesting that only about 3{\%} of the total energy flux of ocean crustal formation is removed by conductive heat transfer, with the remainder being dissipated to overlying seawater by fluid advection.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1525-2027},
	doi = {10.1029/2009GC002957},
	url = {http://dx.doi.org/10.1029/2009GC002957},
	author = {Johnson, H P and Tivey, M A and Bjorklund, T A and Salmi, M S}
}
@article {ISI:000279984900002,
	title = {{Indigenous ectosymbiotic bacteria associated with diverse hydrothermal vent invertebrates}},
	journal = {ENVIRONMENTAL MICROBIOLOGY REPORTS},
	volume = {2},
	number = {4},
	year = {2010},
	month = {aug},
	pages = {479{\textendash}488},
	type = {Review},
	abstract = {P{\textgreater}Symbioses involving bacteria and invertebrates contribute to the biological diversity and high productivity of both aquatic and terrestrial environments. Well-known examples from chemosynthetic deep-sea hydrothermal vent environments involve ectosymbiotic microbes associated with the external surfaces of marine invertebrates. Some of these ectosymbioses confer protection or defence from predators or the environment itself, some are nutritional in nature, and many still are of unknown function. Several recently discovered hydrothermal vent invertebrates, including two populations of yeti crab (Kiwa spp.), a limpet (Symmetromphalus aff. hageni), and the scaly-foot snail (as yet undescribed), support a consortium of diverse bacteria. Comparisons of these ectosymbioses to those previously described revealed similarities among the associated microorganisms, suggesting that certain microbes are indigenous to the surfaces of marine invertebrates. In particular, members of the Thiovulgaceae (epsilonproteobacteria) and Thiotrichaceae (gammaproteobacteria) appear to preferentially form ectosymbioses with vent crustaceans and gastropods. Interactions between specific Proteobacteria and the surfaces of many marine invertebrates likely have ecological and evolutionary significance at these chemically challenging habitats.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {1758-2229},
	doi = {10.1111/j.1758-2229.2010.00136.x},
	author = {Goffredi, Shana K}
}
@article {Cordes2010,
	title = {{Influence of foundation species, depth, and location on diversity and community composition at Gulf of Mexico lower-slope cold seeps}},
	journal = {Deep-Sea Research. Part II: Topical Studies in Oceanography},
	volume = {57},
	number = {21-23},
	year = {2010},
	pages = {1870{\textendash}1881},
	abstract = {Efforts to understand and preserve the seep communities of the deep Gulf of Mexico (GOM) begin with a comprehensive survey of the biodiversity of these communities. Previous studies have provided a conceptual model of the physiology, population, and community ecology of upper continental slope seeps. However, seeps at water depths below 1000 m in the Gulf of Mexico remain relatively unknown. In this study, data from 47 samples of tubeworm- and mussel-associated communities at depths of 1005{\textendash}2750 m are examined. Other than tubeworms and mussels, 66 taxa of macro- and megafauna were collected, 43 of which appear to be restricted to water depths of over 1000 m, and 39 that have not been reported previously from the Gulf of Mexico. Diversity in mussel beds was highest at mid-slope depths, but tubeworm-associated communities did not show clear bathymetric trends in diversity. Diversity was higher in tubeworm aggregations at the alpha level (per sample), but higher in mussel beds at the beta level (species turnover among collections). Although both community types were often numerically dominated by the endemic shrimp Alvinocaris muricola, broad differences in the communities hosted by tubeworm aggregations and mussel beds were apparent. The most important factors explaining community similarity within community type were the depth, relative abundance of different mussel species in a bed, and the average size of tubeworms in an aggregation. The high proportion of deep-seep species that were found for the first time in the Gulf of Mexico emphasizes the importance of conservation efforts for these patchy communities.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0645},
	doi = {10.1016/j.dsr2.2010.05.010},
	url = {http://dx.doi.org/10.1016/j.dsr2.2010.05.010},
	author = {Cordes, E E and Becker, E L and Hourdez, S and Fisher, C R}
}
@article {Feng2010,
	title = {{Initial results of comparing cold-seep carbonates from mussel- and tubeworm-associated environments at Atwater Valley 340, northern Gulf of Mexico}},
	journal = {Deep-Sea Research. Part II: Topical Studies in Oceanography},
	volume = {57},
	number = {21-23},
	year = {2010},
	pages = {2030{\textendash}2039},
	abstract = {Chemosymbiotic macrofauna (such as mussels and tubeworms) and authigenic carbonates are typical of many hydrocarbon seeps. To address whether mussels and tubeworms could impact the sediment geochemistry of their habitat where authigenic carbonates are precipitated, a comparative study of petrographic and geochemical features of the authigenic carbonates from mussel- and tubeworm-associated environments at hydrocarbon seeps in Atwater Valley lease area block 340 (AT340) of the Gulf of Mexico was undertaken. Both mussel- and tubeworm-associated carbonates are dominated by high-magnesium calcite (HMC) and aragonite, and two tubeworm-associated carbonate samples have minor amounts of dolomite. The $δ$13C values of all carbonates are low, ranging from -60.8{\textperthousand} to -35.5{\textperthousand} PDB. Although there is much overlap, surprisingly the $δ$13C values of mussel-associated carbonates are generally higher than those of tubeworm-associated carbonates (-51.8{\textperthousand} vs. -54.8{\textperthousand} for an average of over 60 subsamples). It is suggested that (1) carbon isotopic vital effect of seep mussels and tubeworms, (2) fluid physical pumping of mussels, and (3) release of sulfate by tubeworm roots may be responsible for the relatively lower $δ$13C values of tubeworm-associated carbonates. It has been suggested that the heterogeneities in mineralogy and stable carbon isotope geochemistry of the seep carbonates may be attributed to the activity of macrofauna (mussels and tubeworms) and associated microbes. Our observations also suggest that at AT340 the geochemical evolution of seep macrofauna is from a mussel-dominated environment to a mixed mussel-tubeworm environment, and finally to a mostly tubeworm-dominated environment. This evolution is controlled mainly by the habitat, e.g., hydrocarbon seep flux.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0645},
	doi = {10.1016/j.dsr2.2010.05.004},
	url = {http://dx.doi.org/10.1016/j.dsr2.2010.05.004},
	author = {Feng, D and Roberts, H H}
}
@article {Barker2010,
	title = {{Insights into the behaviour of sulphur in mid-ocean ridge axial hydrothermal systems from the composition of the sheeted dyke complex at Pito Deep}},
	journal = {Chemical Geology},
	volume = {275},
	number = {1-2},
	year = {2010},
	pages = {105{\textendash}115},
	abstract = {The behaviour of seawater sulphate in hydrothermal systems at intermediate- to fast-spreading ridges is investigated using new analyses of the $δ$34S, sulphur concentration and Fe2O3/Fe2O3total, combined with existing 87Sr/86Sr, of sheeted dykes from the Pito Deep tectonic window. The Pito Deep sheeted dyke complex has a similar composition to the sheeted dykes drilled at ODP Hole 504B suggesting that the measured compositions are representative of sheeted dyke complexes at intermediate- to fast-spreading ridges. The dykes show only small increases in $δ$34S which, combined with the rock dominated $δ$34S of vent fluids, requires the majority of seawater sulphate to be precipitated as anhydrite before the fluid reacts with the sheeted dyke complex. This loss of sulphate from the fluid means that a much higher Fe2O3 in the sheeted dyke complex than in fresh MORB glasses cannot be explained by oxidation due to seawater sulphate reduction during fluid{\textendash}rock reaction. Instead, oxidation probably occurs due to degassing of reduced species, largely H2, during dyke emplacement and solidification. A mass balance model that accounts for anhydrite precipitation and Sr partitioning into the anhydrite, as well as fitting the concentration and isotopic ratios of S and Sr in the sheeted dykes and vent fluids, suggests water/rock ratios of \~{} 1. For a 1 km thick sheeted dyke complex this is equivalent to a fluid flux of \~{} 3 {\texttimes} 106 kg m- 2, sufficient to remove \~{} 60{\%} of the latent heat of crystallization from the lower crust.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0009-2541},
	doi = {10.1016/j.chemgeo.2010.05.003},
	url = {http://dx.doi.org/10.1016/j.chemgeo.2010.05.003},
	author = {Barker, A K and Coogan, L A and Gillis, K M}
}
@article {Craddock2010,
	title = {{Insights to magmatic{\textendash}hydrothermal processes in the Manus back{\textendash}arc Basin as recorded by anhydrite}},
	journal = {Geochimica et Cosmochimica Acta},
	volume = {74},
	number = {19},
	year = {2010},
	pages = {5514{\textendash}5536},
	abstract = {Microchemical analyses of rare earth element (REE) concentrations and Sr and S isotope ratios of anhydrite are used to identify sub-seafloor processes governing the formation of hydrothermal fluids in the convergent margin Manus Basin, Papua New Guinea. Samples comprise drill-core vein anhydrite and seafloor massive anhydrite from the PACMANUS (Roman Ruins, Snowcap and Fenway) and SuSu Knolls (North Su) active hydrothermal fields. Chondrite-normalized REE patterns in anhydrite show remarkable heterogeneity on the scale of individual grains, different from the near uniform REEN patterns measured in anhydrite from mid-ocean ridge deposits. The REEN patterns in anhydrite are correlated with REE distributions measured in hydrothermal fluids venting at the seafloor at these vent fields and are interpreted to record episodes of hydrothermal fluid formation affected by magmatic volatile degassing. 87Sr/86Sr ratios vary dramatically within individual grains between that of contemporary seawater and that of endmember hydrothermal fluid. Anhydrite was precipitated from a highly variable mixture of the two. The intra-grain heterogeneity implies that anhydrite preserves periods of contrasting hydrothermal versus seawater dominant near-seafloor fluid circulation. Most sulfate $δ$34S values of anhydrite cluster around that of contemporary seawater, consistent with anhydrite precipitating from hydrothermal fluid mixed with locally entrained seawater. Sulfate $δ$34S isotope ratios in some anhydrites are, however, lighter than that of seawater, which are interpreted as recording a source of sulfate derived from magmatic SO2 degassed from underlying felsic magmas in the Manus Basin. The range of elemental and isotopic signatures observed in anhydrite records a range of sub-seafloor processes including high-temperature hydrothermal fluid circulation, varying extents of magmatic volatile degassing, seawater entrainment and fluid mixing. The chemical and isotopic heterogeneity recorded in anhydrite at the inter- and intra-grain scale captures the dynamics of hydrothermal fluid formation and sub-seafloor circulation that is highly variable both spatially and temporally on timescales over which hydrothermal deposits are formed. Microchemical analysis of hydrothermal minerals can provide information about the temporal history of submarine hydrothermal systems that are variable over time and cannot necessarily be inferred only from the study of vent fluids.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0016-7037},
	doi = {10.1016/j.gca.2010.07.004},
	url = {http://dx.doi.org/10.1016/j.gca.2010.07.004},
	author = {Craddock, P R and Bach, W}
}
@article {ISI:000280633000011,
	title = {{Isolated communities of Epsilonproteobacteria in hydrothermal vent fluids of the Mariana Arc seamounts}},
	journal = {FEMS MICROBIOLOGY ECOLOGY},
	volume = {73},
	number = {3},
	year = {2010},
	month = {sep},
	pages = {538{\textendash}549},
	type = {Article},
	abstract = {Low-temperature hydrothermal vent fluids represent access points to diverse microbial communities living in oceanic crust. This study examined the distribution, relative abundance, and diversity of Epsilonproteobacteria in 14 low-temperature vent fluids from five volcanically active seamounts of the Mariana Arc using a 454 tag sequencing approach. Most vent fluids were enriched in cell concentrations compared with background seawater, and quantitative PCR results indicated that all fluids were dominated by bacteria. Operational taxonomic unit-based statistical tools applied to 454 data show that all vents from the northern end of the Mariana Arc grouped together, to the exclusion of southern arc seamounts, which were as distinct from one another as they were from northern seamounts. Statistical analysis also showed a significant relationship between seamount and individual vent groupings, suggesting that community membership may be linked to geographical isolation and not geochemical parameters. However, while there may be large-scale geographic differences, distance is not the distinguishing factor in the microbial community composition. At the local scale, most vents host a distinct population of Epsilonproteobacteria, regardless of seamount location. This suggests that there may be barriers to exchange and dispersal for these vent endemic microorganisms at hydrothermal seamounts of the Mariana Arc.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0168-6496},
	doi = {10.1111/j.1574-6941.2010.00910.x},
	author = {Huber, Julie A and Cantin, Holly V and Huse, Susan M and Welch, David B Mark and Sogin, Mitchell L and Butterfield, David A}
}
@article {ISI:000277252000001,
	title = {{Malaguana-Gadao Ridge: Identification and implications of a magma chamber reflector in the southern Mariana Trough}},
	journal = {GEOCHEMISTRY GEOPHYSICS GEOSYSTEMS},
	volume = {11},
	year = {2010},
	month = {apr},
	type = {Article},
	abstract = {Six-channel seismic reflection data reveal a magma chamber reflector beneath the Malaguana-Gadao Ridge, the southernmost segment of the spreading center in the Mariana Trough. For most of its length the spreading center in this active back-arc basin is morphologically similar to slow spreading mid-ocean ridges, having a deep central graben flanked by a zone of abyssal hill fabric. This southernmost segment, however, has a broad, smooth cross section, lacks a deep central graben, and is thus similar in morphology to fast spreading ridges (e.g., the East Pacific Rise). We identify a magma chamber at 1.5 s two-way travel time below the crest of the ridge. Observations from remotely operated vehicles along the ridge reveal not only fresh pillows, lobate, and sheet lava flows but also an abundance of volcaniclastic debris and intense hydrothermal activity. These observations, together with the {\textquoteleft}{\textquoteleft}fast spreading{\{}{\textquoteright}{\textquoteright}{\}} morphology of the ridge, suggest that this segment has a considerably higher magma supply than is typical in the Mariana Trough. We suggest that the volcanic arc or enhanced melting of a hydrated mantle is supplying volatile-rich magma as evidenced by a highly negative coefficient of reflection, -0.42, for this MCR and the presence of evolved, highly vesicular lava and volcaniclastic materials. The southeastern Mariana back-arc basin spreading ridge does not compare readily with mechanical models for global mid-ocean ridge data sets because of marked asymmetry in both volcanism and deformation that may be the consequence of slab-related geometry in this part of the convergent margin system.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1525-2027},
	doi = {10.1029/2009GC002719},
	author = {Becker, Nathan C and Fryer, Patricia and Moore, Gregory F}
}
@article {Dick2010,
	title = {{Mantle melting, melt transport, and delivery beneath a slow-spreading ridge: the paleo-MAR from 23 degrees15'N to 23 degrees 45'N}},
	journal = {Journal of Petrology},
	volume = {51},
	number = {1-2},
	year = {2010},
	pages = {425{\textendash}467},
	abstract = {Kane Megamullion, an oceanic core complex near the Mid-Atlantic Ridge (MAR) abutting the Kane Transform, exposes nearly the full plutonic foundation of the MARK paleo-ridge segment. This provides the first opportunity for a detailed look at the patterns of mantle melting, melt transport and delivery at a slow-spreading ridge. The Kane lower crust and mantle section is heterogeneous, as a result of focused mantle melt flow to different points beneath the ridge segment in time and space, over an \~{}300{\textendash}400 kyr time scale. The association of residual mantle peridotite, dunite and troctolite with a large \~{}1 km+ thick gabbro section at the Adam Dome Magmatic Center in the southern third of the complex probably represents the crust{\textendash}mantle transition. This provides direct evidence for local melt accumulation in the shallow mantle near the base of the crust as a result of dilation accompanying corner flow beneath the ridge. Dunite and troctolite with high-Mg Cpx represent melt{\textendash}rock reaction with the mantle, and suggest that this should be taken into account in modeling the evolution of mid-ocean ridge basalt (MORB). Despite early precipitation of high-Mg Cpx, wehrlites similar to those in many ophiolites were not found. Peridotite modes from the main core complex and transform wall define a depletion trend coincident with that for the SW Indian Ridge projecting toward East Pacific Rise mantle exposed at Hess Deep. The average Kane transform peridotite is a lherzolite with 5{\textperiodcentered}2{\%} Cpx, whereas that from the main core complex is a harzburgite with only 3{\textperiodcentered}5{\%} Cpx. As the area corresponds to a regional bathymetric low, and the crust is apparently thin, it is likely that most residual mantle along the MAR is significantly more depleted. Thus, harzburgitic and lherzolitic ophiolite subtypes cannot be simply interpreted as slow- and fast-spreading ridges respectively. The mantle peridotites are consistent with a transform edge effect caused by juxtaposition of old cold lithosphere against upwelling mantle at the ridge{\textendash}transform intersection. This effect is far more local, confined to within 10 km of the transform slip zone, and far smaller than previously thought, corresponding to \~{}8{\%} as opposed to 12{\textperiodcentered}5{\%} melting of a pyrolitic mantle away from the transform. Excluding the transform, the overall degree of melting over 3 Myr indicated by the peridotites is uniform, ranging from \~{}11{\textperiodcentered}3 to 13{\textperiodcentered}8{\%}. Large variations in composition for a single dredge or ROV dive, however, reflect local melt transport through the shallow mantle. This produced variable extents of melt{\textendash}rock reaction, dunite formation, and melt impregnation. At least three styles of late mantle metasomatism are present. Small amounts of plagioclase with elevated sodium and titanium and alumina-depletion in pyroxene relative to residual spinel peridotites represent impregnation by a MORB-like melt. Highly variable alumina depletion in pyroxene rims in spinel peridotite probably represents cryptic metasomatism by small volumes of late transient silica-rich melts meandering through the shallow mantle. Direct evidence for such melts is seen in orthopyroxenite veins. Finally, a late hydrous fluid may be required to explain anomalous pyroxene sodium enrichment in spinel peridotites. The discontinuous thin lower crust exposed at Kane Megamullion contrasts with the {\textgreater}700 km2 1{\textperiodcentered}5 km+ thick Atlantis Bank gabbro massif at the SW Indian Ridge (SWIR), clearly showing more robust magmatism at the latter. However, the SWIR spreading rate is 54{\%} of the MAR rate, the offset of the Atlantis II Fracture Zone is 46{\%} greater and Na8 of the spatially associated basalts 16{\%} greater{\textemdash}all of which predict precisely the opposite. At the same time, the average compositions of Kane and Atlantis II transform peridotites are nearly identical. This is best explained by a more fertile parent mantle beneath the SWIR and demonstrates that crustal thickness predicted by simply inverting MORB compositions can be significantly in error.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0022-3530},
	doi = {10.1093/petrology/egp088},
	url = {http://dx.doi.org/10.1093/petrology/egp088},
	author = {Dick, H J B and Lissenberg, C J and Warren, J M}
}
@article {Yeats2010,
	title = {{Mapping elemental distributions in submarine hydrothermal sulfide smokers using proton induced X-ray emission}},
	journal = {Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms},
	volume = {268},
	number = {11-12},
	year = {2010},
	pages = {2129{\textendash}2132},
	abstract = {PIXE analysis using a 3 MeV proton beam on the CSIRO Nuclear Microprobe was carried out on samples of four typical undersea sulfide chimneys from the Rogers Ruins and Fenway hydrothermal sites, PACMANUS field, Eastern Manus Basin, Papua New Guinea. The ability of PIXE to map the spatial association of trace elements within the sulfides across multiple mineralogical zones provides important insights into the mode of formation of structures and the nature of interaction between \~{}250 and 350 {\textdegree}C hydrothermal fluids and 3{\textendash}4 {\textdegree}C ambient seawater within the chimney walls.},
	keywords = {AUV ABE (Autonomous Benthic Explorer), ROV Jason (Remotely Operated Vehicle)},
	issn = {0168-583X},
	doi = {10.1016/j.nimb.2010.02.034},
	url = {http://dx.doi.org/10.1016/j.nimb.2010.02.034},
	author = {Yeats, C and Belton, D and Laird, J S and Ryan, C G}
}
@article {Herrera2010,
	title = {{Molecular systematics of the bubblegum coral genera (Paragorgiidae, Octocorallia) and description of a new deep-sea species}},
	journal = {Molecular Phylogenetics and Evolution},
	volume = {55},
	number = {1},
	year = {2010},
	pages = {123{\textendash}135},
	abstract = {Bubblegum octocorals (Paragorgia and Sibogagorgia) play an important ecological role in many deep-sea ecosystems. However, these organisms are currently threatened by destructive fishing methods such as bottom trawling. Taxonomic knowledge of conservation targets is necessary for the creation and implementation of efficient conservation strategies. However, for most deep-sea coral groups this knowledge remains incomplete. For instance, despite its similarities with Paragorgia, Sibogagorgia is particular in lacking polyp sclerites, which are present in groups like Paragorgia and the Coralliidae. Although two kinds of sclerites are very similar between Paragorgia and Sibogagorgia, other characters challenge the monophyly of these genera. Here we help to clarify the taxonomy and evolutionary relationships of the bubblegum octocorals and related taxa by examining molecular data. We employed nucleotide sequences of mitochondrial (ND6, ND6-ND3 intergenic spacer, ND3, ND2, COI, msh1 and 16S) and nuclear (28S and ITS2) genomic regions from several taxa to infer molecular phylogenetics and to examine the correspondence of morphological features with the underlying genetic information. Our data strongly supported the monophyly of the genus Paragorgia, the family Coralliidae (precious corals), and a group of undescribed specimens resembling Sibogagorgia. Further morphological observations were congruent regarding the uniqueness of the undescribed specimens, here defined as a new species, Sibogagorgia cauliflora sp. nov., which occurs in both sides of the North American landmass at depths below 1700 m. This new species resembles S. dennisgordoni with branching in one plane but has fairly different radiate sclerites and significantly divergent DNA sequences. The existence of several diagnostic characters of Sibogagorgia in S. cauliflora indicates that they indeed belong to this genus. It is however remarkable that a small number of medullar canals are also found in this species; medullar canals have been considered as the main diagnostic character of Paragorgia. Thus, the evidence generated here indicates that the presence or absence of these canals per se is not a conclusively diagnostic character for either genus. The lack of internal-node resolution in the inferred phylogenetic hypotheses of these genera does not allow us to propose a clear scenario regarding the evolution of these traits.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1055-7903},
	doi = {10.1016/j.ympev.2009.12.007},
	url = {http://dx.doi.org/10.1016/j.ympev.2009.12.007},
	author = {Herrera, S and Baco, A R and Sanchez, J A}
}
@article {Wankel2010,
	title = {{New constraints on methane fluxes and rates of anaerobic methane oxidation in a Gulf of Mexico brine pool via in situ mass spectrometry}},
	journal = {Deep-Sea Research. Part II: Topical Studies in Oceanography},
	volume = {57},
	number = {21-23},
	year = {2010},
	pages = {2022{\textendash}2029},
	abstract = {Deep-sea biogeochemical cycles are, in general, poorly understood owing to the difficulties of making measurements in situ, recovering samples with minimal perturbation, and, in many cases, coping with high spatial and temporal heterogeneity. In particular, biogeochemical fluxes of volatiles such as methane remain largely unconstrained because of the difficulties with accurate quantification in situ and the patchiness of point sources such as seeps and brine pools. To better constrain biogeochemical fluxes and cycling, we have developed a deep-sea in situ mass spectrometer (ISMS) to enable high-resolution quantification of volatiles in situ. Here we report direct measurements of methane concentrations made in a Gulf of Mexico brine pool located at a depth of over 2300 m. Concentrations of up to 33 mM methane were observed within the brine pool, whereas concentrations in the water directly above were three orders of magnitude lower. These direct measurements enabled us to make the first accurate estimates of the diffusive flux from a brine pool, calculated to be 1.1{\textpm}0.2 mol m-2 yr-1. Integrated rate measurements of aerobic methane oxidation in the water column overlying the brine pool were \~{}320 $μ$mol m-2 yr-1, accounting at most for just 0.03{\%} of the diffusive methane flux from the brine pool. Calculated rates of anaerobic methane oxidation were 600{\textendash}1200 $μ$M yr-1, one to two orders of magnitude higher than previously published values of AOM in anoxic fluids. These findings suggest that brine pools are enormous point sources of methane in the deep sea, and may, in aggregate, have a pronounced impact on the global marine methane cycle.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0645},
	doi = {10.1016/j.dsr2.2010.05.009},
	url = {http://dx.doi.org/10.1016/j.dsr2.2010.05.009},
	author = {Wankel, S D and Joye, S B and Samarkin, V A and Shah, S R and Friederich, G and Melas-Kyriazi, J and Girguis, P R}
}
@article {ISI:000208389800002,
	title = {{A new coral species introduced into the Atlantic Ocean - Tubastraea micranthus (Ehrenberg 1834) (Cnidaria, Anthozoa, Scleractinia): An invasive threat?}},
	journal = {AQUATIC INVASIONS},
	volume = {5},
	number = {2},
	year = {2010},
	month = {jun},
	pages = {131{\textendash}140},
	type = {Article},
	abstract = {Over the past 60-70 years, the invasive Indo-Pacific coral Tubastraea coccinea (Lesson 1830; Cnidaria, Scleractinia) has colonized the western tropical Atlantic Ocean - the Americas, the Antilles, northern Gulf of Mexico (GOM), and many of its 3,600 oil/gas platforms. It is now the single, most abundant coral on artificial substrata in the GOM, with hundreds of thousands of colonies on a single platform. Here, we report for the first time the observation of a closely related congener in the western Atlantic - the Indo-Pacific azooxanthellate Tubastraea micranthus (Ehrenberg 1834) - and suggest that it may pose a threat similar to T. coccinea. A total of 83 platforms, including deep-water, toppled, Rigs-to-Reefs structures, were surveyed in the northern Gulf of Mexico between 2000 and 2009, from Matagorda Island, Texas to Mobile, Alabama, USA, between the depths of 7 and 37 m, by SCUBA divers. Five platforms were surveyed by Remotely Operated Vehicle (ROV) to depths of up to 117 m. T. micranthus was found on only one platform - Grand Isle 93 (GI-93), off Port Fourchon, Louisiana, near the Mississippi River mouth, at the cross-roads of two major safety fairways/shipping lanes transited by large international commercial ships. The introduction appears to be recent, probably derived from the ballast water or hull of a vessel from the Indo-Pacific. If the growth and reproductive rates of T. micranthus, both sexual and asexual, are similar to those of T. coccinea, this species could dominate this region like its congener. It is not known whether this species is an opportunist/pioneer species, like T. coccinea, a trait protecting benthic communities from its dominance. The question of rapid-response eradication is raised.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1798-6540},
	doi = {10.3391/ai.2010.5.2.02},
	author = {Sammarco, Paul W and Porter, Scott A and Cairns, Stephen D}
}
@article {Hulme2010,
	title = {{Pore water chemistry of the Mariana serpentinite mud volcanoes: A window to the seismogenic zone}},
	journal = {Geochemistry, Geophysics, Geosystems},
	volume = {11},
	year = {2010},
	pages = {Q01X09},
	abstract = {In 2003, we conducted a survey of 11 serpentinite mud volcanoes in the Mariana fore arc. Here we report sediment pore water data from navigated gravity and piston cores and from push cores collected by the ROV system Jason2-Medea. Systematic variations in profiles of pore water chemical compositions from these mud volcanoes are consistent with models that include active upflow of pore water relative to the surrounding serpentinite matrix. The speed of upwelling, based on fits of an advection-diffusion model to observed data (K, Na, Rb, and Cs), reaches a maximum of 36 cm/yr at Big Blue Seamount. Results from these simulations constrain the pore water composition at depth and the degree of additional alteration as the pore water ascends through the sampled section. For example, the transition metals (e.g., Mn, Fe, Co, Ni, Cu, and Mo) are mobilized under conditions of low upwelling speeds and microbial activity. Similarly, the rare earth elements (REE) show evidence of near-surface alteration. In addition to these surficial reactions, distinctive pore water compositional patterns exist as a function of the distance from the trench axis, which is a proxy for the depths of water generation from the downgoing plate below each seamount. Systematic trends in the chemical composition of these slab-sourced fluids are consistent with increasing temperature and pressure at depth west of the trench. These trends include an increase in K, sulfate, carbonate alkalinity, Na/Cl, B, Mn, Fe, Co, Rb, Cs, Gd/Tb, Eu, and light REE (LREE) and a decrease in Ca, Sr, and Y with increasing distance from the trench. Mg and U are universally depleted in the upwelling water. We constrain the thermal conditions along the d{\'e}collement using concentrations of fluid mobile elements (K, B, Cs, and Rb) and the mobilization of LREE relative to heavy REE (HREE). The 80{\textdegree}C isotherm is estimated at a depth of 15 km between Blue Moon Seamount and Cerulean Springs. At slab depths of 17 to 24 km, pore waters lack significant Rb and K enrichments relative to seawater, suggesting an upper bound near 150{\textdegree}C. There is an observed enrichment in LREE relative to HREE at Big Blue Seamount (slab depth 25 km) indicating that the d{\'e}collement at this site is \~{}200{\textdegree}C. The relative mobilization of Cs outpaced that of Rb at all seamounts sampled in this survey. On the basis of laboratory experiments, this observation sets an upper limit of \~{}350{\textdegree}C at a depth of 30 km below the seafloor.},
	keywords = {ROV Jason (Remotely Operated Vehicle), ROV Medea (Remotely Operated Vehicle)},
	issn = {1525-2027},
	doi = {10.1029/2009GC002674},
	url = {http://dx.doi.org/10.1029/2009GC002674},
	author = {Hulme, S M and Wheat, C G and Fryer, P and Mottl, M J}
}
@article {Craddock2010a,
	title = {{Rare earth element abundances in hydrothermal fluids from the Manus Basin, Papua New Guinea: indicators of sub-seafloor hydrothermal processes in back-arc basins}},
	journal = {Geochimica et Cosmochimica Acta},
	volume = {74},
	number = {19},
	year = {2010},
	pages = {5494{\textendash}5513},
	abstract = {Rare earth element (REE) concentrations are reported for a large suite of seafloor vent fluids from four hydrothermal systems in the Manus back-arc basin (Vienna Woods, PACMANUS, DESMOS and SuSu Knolls vent areas). Sampled vent fluids show a wide range of absolute REE concentrations and chondrite-normalized (REEN) distribution patterns (LaN /SmN \~{} 0.6{\textendash}11; LaN /YbN \~{} 0.6 {\textendash} 71; View the MathML sourceEuN/EuN*\~{}1{\textendash}55). REEN distribution patterns in different vent fluids range from light-REE enriched, to mid- and heavy-REE enriched, to flat, and have a range of positive Eu-anomalies. This heterogeneity contrasts markedly with relatively uniform REEN distribution patterns of mid-ocean ridge hydrothermal fluids. In Manus Basin fluids, aqueous REE compositions do not inherit directly or show a clear relationship with the REE compositions of primary crustal rocks with which hydrothermal fluids interact. These results suggest that the REEs are less sensitive indicators of primary crustal rock composition despite crustal rocks being the dominant source of REEs in submarine hydrothermal fluids. In contrast, differences in aqueous REE compositions are consistently correlated with differences in fluid pH and ligand (chloride, fluoride and sulfate) concentrations. Our results suggest that the REEs can be used as an indicator of the type of magmatic acid volatile (i.e., presence of HF, SO2) degassing in submarine hydrothermal systems. Additional fluid data suggest that near-seafloor mixing between high-temperature hydrothermal fluid and locally entrained seawater at many vent areas in the Manus Basin causes anhydrite precipitation. Anhydrite effectively incorporates REE and likely affects measured fluid REE concentrations, but does not affect their relative distributions.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0016-7037},
	doi = {10.1016/j.gca.2010.07.003},
	url = {http://dx.doi.org/10.1016/j.gca.2010.07.003},
	author = {Craddock, P R and Bach, W and Seewald, J S and Rouxel, O J and Reeves, E and Tivey, M K}
}
@article {Garcia-Pineda2010,
	title = {{Remote-sensing evaluation of geophysical anomaly sites in the outer continental slope, northern Gulf of Mexico}},
	journal = {Deep-Sea Research. Part II: Topical Studies in Oceanography},
	volume = {57},
	number = {21-23},
	year = {2010},
	pages = {1859{\textendash}1869},
	abstract = {Synthetic Aperture Radar (SAR) images obtained from satellites are a reliable tool for localizing natural hydrocarbon seeps. For this study, we used the Texture Classifier Neural Network Algorithm (TCNNA) to interpret SAR data from the RADARSAT satellite and a geostatistical clustering analysis to compare seeps detected in 579 SAR images covering the northern Gulf of Mexico (GOM). Geostatistical analysis results indicate that, in a typical active seep formation, oil vents would be found within a seep formation \~{}2.5 km in diameter. Repeated observations of slicks at a given seep formation indicate that advection of rising oil in the water column causes an offset from the vent depending on water depth. At 500 m, the radial offset is up to 1400 m; at 2000 m, it is up to 3270 m. Observations with submersibles showed that, in 100{\%} of the cases, the calculated seep formations that are matched with active oil seeps correspond to anomalies interpreted from surface amplitude maps and migration pathways in the seismic data. However, episodically, larger releases from persistent seeps occurred, and also some other seep formations showed intermittent releases. Our analysis indicates that active oil seeps detected with SAR represent a subset of the total array of geophysical features generated by hydrocarbon migration on the northern continental slope of the Gulf of Mexico.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0645},
	doi = {10.1016/j.dsr2.2010.05.005},
	url = {http://dx.doi.org/10.1016/j.dsr2.2010.05.005},
	author = {Garcia-Pineda, O and MacDonald, I R and Zimmer, B and Shedd, B and Roberts, H}
}
@article {1452,
	title = {{ROV records deepest explosive eruption on the seafloor}},
	journal = {Sea Technology},
	volume = {51},
	number = {2},
	year = {2010},
	pages = {83},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0093-3651}
}
@article {Miglietta2010,
	title = {{Species boundaries of Gulf of Mexico vestimentiferans (Polychaeta, Siboglinidae) inferred from mitochondrial genes}},
	journal = {Deep-Sea Research. Part II: Topical Studies in Oceanography},
	volume = {57},
	number = {21-23},
	year = {2010},
	pages = {1916{\textendash}1925},
	abstract = {At least six morphospecies of vestimentiferan tubeworms are associated with cold seeps in the Gulf of Mexico (GOM). The physiology and ecology of the two best-studied species from depths above 1000 m in the upper Louisiana slope (Lamellibrachia luymesi and Seepiophila jonesi) are relatively well understood. The biology of one rare species from the upper slope (escarpiid sp. nov.) and three morphospecies found at greater depths in the GOM (Lamellibrachia sp. 1, L. sp. 2, and Escarpia laminata) are not as well understood. Here we address species distributions and boundaries of cold-seep tubeworms using phylogenetic hypotheses based on two mitochondrial genes. Fragments of the mitochondrial large ribosomal subunit rDNA (16S) and cytochrome oxidase subunit I (COI) genes were sequenced for 167 vestimentiferans collected from the GOM and analyzed in the context of other seep vestimentiferans for which sequence data were available. The analysis supported five monophyletic clades of vestimentiferans in the GOM. Intra-clade variation in both genes was very low, and there was no apparent correlation between the within-clade diversity and collection depth or location. Two of the morphospecies of Lamellibrachia from different depths in the GOM could not be distinguished by either mitochondrial gene. Similarly, E. laminata could not be distinguished from other described species of Escarpia from either the west coast of Africa or the eastern Pacific using COI. We suggest that the mitochondrial COI and 16S genes have little utility as barcoding markers for seep vestimentiferan tubeworms.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0645},
	doi = {10.1016/j.dsr2.2010.05.007},
	url = {http://dx.doi.org/10.1016/j.dsr2.2010.05.007},
	author = {Miglietta, M P and Hourdez, S and Cowart, D A and Schaeffer, S W and Fisher, C R}
}
@article {Becker2010,
	title = {{Stable carbon and nitrogen isotope compositions of hydrocarbon-seep bivalves on the Gulf of Mexico lower continental slope}},
	journal = {Deep-Sea Research. Part II: Topical Studies in Oceanography},
	volume = {57},
	number = {21-23},
	year = {2010},
	pages = {1957{\textendash}1964},
	abstract = {Stable isotope compositions of cold-seep bivalves can illuminate processes that affect the chemical and isotopic compositions of seeping fluids along the continental slope of the Gulf of Mexico as well as provide insight into the physiological ecology of these species. Carbon and nitrogen isotope compositions were analyzed in mussels and clams from 14 seep sites spanning a depth range of 1000 to 2800 m along the lower Louisiana slope of the Gulf of Mexico. Mussels of three species found on the lower slope, Bathymodiolus childressi, B. brooksi, and B. heckerae, showed site-specific differences in tissue $δ$13C, reflecting differences in the local methane pool. Mussels from sites on the lower slope sitting atop the contiguous salt sheet generally had tissue $δ$13C values that reflected a stronger biogenic methane signal (-70.8 to -58.8{\textperthousand}) than mussels on the upper slope or seaward of the Sigsbee Escarpment (-67.3 to -40.4{\textperthousand}). Clams (Calyptogena ponderosa and Calyptogena sp. nov.) had a narrow range of $δ$13C values between -37.0 and -34.4{\textperthousand}, indicating that their thiotrophic symbionts are fixing primarily seawater-dissolved organic carbon. The most depleted tissue $δ$15N values yet published for both mussels and clams are reported in this study at -23.7 and -9.2{\textperthousand}, respectively. These depleted values have implications for the assimilation of inorganic nitrogen by these symbioses and the concentrations of particular inorganic nitrogen sources in the local environment.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0645},
	doi = {10.1016/j.dsr2.2010.05.002},
	url = {http://dx.doi.org/10.1016/j.dsr2.2010.05.002},
	author = {Becker, E L and Lee, R W and Macko, S A and Faure, B M and Fisher, C R}
}
@article {Carney2010,
	title = {{Stable isotope trophic patterns in echinoderm megafauna in close proximity to and remote from Gulf of Mexico lower slope hydrocarbon seeps}},
	journal = {Deep-Sea Research. Part II: Topical Studies in Oceanography},
	volume = {57},
	number = {21-23},
	year = {2010},
	pages = {1965{\textendash}1971},
	abstract = {Hydrocarbon-seep communities in the Gulf of Mexico have a high biomass that is exploited as a food source to varying degrees by the photosynthesis-dependent fauna inhabiting the surrounding mud bottom. A decline concurrent with ocean depth in detritus influx to that background habitat results in a much lower background biomass. The biomass contrast between population-rich seeps and depauperate mud bottom leads to the prediction that seep utilization by the background fauna should be extensive at all depths and should increase with depth. Species depth zonation makes like-species comparisons over the full depth of the Gulf of Mexico impossible. Seeps and normal bottom above 1000 m have different fauna from those below 1000 m. Lower slope seeps are surrounded by a fauna rich in echinoderm species, especially asteroids, ophiuroids, and holothuroids. All three taxa have species that are abundant within seeps and are probably endemic to them. They also contain species found only in mud background or within mud and seeps backgrounds. Tissue analyses of $δ$13C and $δ$15N of echinoderms collected by ROV within seeps and trawling away from seeps indicate a pattern of utilization similar to that found in upper slope seeps exploited by different taxa. Seastar and ophiuroid species abundant in or endemic to seeps have tissue isotope values reflecting seep chemosynthetic input via a free-living microbial detritus or predation. A single seep-endemic deposit-feeding holothuroid showed distinct seep tissue values. Background deposit-feeding holothuroids collected within seeps showed either no or only minor incorporation of seep carbon, indicating either a lack of access to seep detritus or short feeding times within the seep. A predicted extensive utilization of seep productivity at the deeper seeps was not found. Seeps may be relatively closed systems that require special adaptations of species in order for them to enter, exploit, and survive. Alternately, the surrounding deep benthos may not be as food-poor as assumed from biomass measurements and flux estimates.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0645},
	doi = {10.1016/j.dsr2.2010.09.027},
	url = {http://dx.doi.org/10.1016/j.dsr2.2010.09.027},
	author = {Carney, R S}
}
@mastersthesis {Nakata2010,
	title = {{Syn-eruptive degassing of a single submarine lava flow : constraints on MORB CO2 variability, vesiculation, and eruption dynamics}},
	volume = {M.S.},
	year = {2010},
	pages = {63},
	school = {Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution},
	type = {phd},
	address = {Cambridge, MA and Woods Hole, MA},
	abstract = {Mid-ocean ridge basalts (MORBs) exhibit a wide range of CO2 concentrations, reflecting saturation to supersaturation (and rarely undersaturation) relative to their emplacement depths. In this study, we explore the mechanisms of CO2 degassing and the implications this has for estimating the advance rates and durations of seafloor eruptions. We present dissolved volatile concentrations (mainly of CO2 and H2O) and vesicle size distributions (VSDs) for a unique suite of MORB glasses collected at the East Pacific Rise, {\~{}}9{\textdegree} 50' N. These MORB glasses were collected at {\~{}}200 m intervals along an across-axis track over a single flow pathway within the recently emplaced 2005-06 eruption boundaries; systematic sample collection provides one of the first opportunities to characterize intra-flow geochemical and physical evolution during a single eruption at a fast-spreading ridge. Compared to measurements of MORB volatiles globally, dissolved H2O concentrations are relatively uniform (0.10 - 0.16 weight percent), whereas dissolved CO2 contents exhibit a range of concentrations (154 - 278 ppm) and decrease with distance from the EPR axis (i.e., eruptive vent). Ion microprobe analyses of dissolved volatiles within the MORB glasses suggest that the magma erupted supersaturated (pressure equilibrium with 920 - 1224 mbsf) and in near-equilibrium with the melt lens of the axial magma chamber ({\~{}}1250 - 1500 mbsf), and degassed to near equilibrium (299 - 447 mbsf) with seafloor depths over the length of the flow. The decrease in CO2 concentrations spans nearly the full range of dissolved CO2 contents observed at the EPR and shows that the varying degrees of volatile saturation that have been observed in other MORB sample suites may be explained by degassing during emplacement. Vesicularity (0.1 - 1.2{\%}) increases with decreasing dissolved CO2 concentrations. We use vesicle size distributions (VSDs){\textemdash}vesicle sizes and number densities{\textemdash}to quantify the physical evolution of the CO2 degassing process. VSDs suggest that diffusion of CO2 into preexisting vesicles, and not nucleation of new vesicles, is the dominant mechanism of increasing CO2 in the vapor phase. We also use VSDs, along with estimates of vesicle growth rates, to constrain emplacement time of the 2005-06 eruption to {\textless}{\~{}}24 hours and to resolve variations in advance rate with downflow distance.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1575/1912/3933},
	url = {http://hdl.handle.net/1912/3933},
	author = {Nakata, D S}
}
@article {Thresher2010,
	title = {{Temperature effects on the calcite skeletal composition of deep-water gorgonians (isididae)}},
	journal = {Geochimica et Cosmochimica Acta},
	volume = {74},
	number = {16},
	year = {2010},
	pages = {4655{\textendash}4670},
	abstract = {We test for and calibrate a proxy for ocean temperature based on the skeletal composition of the widely distributed, deep-sea gorgonians in the family Isididae (bamboo corals), through use of three complementary methods: a short-term comparison of element/Ca ratios to a four-year temperature record, a long-term comparison with oceanographic records spanning forty years, and a geographic comparison of Isidids collected at sites ranging from the tropics to Antarctica. The assays consistently support a temperature-dependency for Mg/Ca ratios and suggest S/Ca is indirectly affected by temperature, but indicate little or no effect of temperature on P/Ca and Sr/Ca. The consensus relationship between Mg/Ca and temperature for Isidid calcite from the comparisons with the temperature time-series is T = -0.505 + 0.048 Mg/Ca, where T is in {\textdegree}C, Mg/Ca is in mmol/mol, and the applicable range is 3{\textendash}6 {\textdegree}C. The results of the geographic assay, though imprecise, suggest the applicable range extends to temperatures below freezing. The scatter of data points around the regression of temperature and Mg/Ca is wide in all assays. This could reflect the effect of factors other than temperature on Mg/Ca ratios, but is also likely to reflect limitations of the field data, the effects of assumed constant growth rates in the corals and instrumental analytical error. The combined effects of micro-scale variability in growth rates and wide confidence intervals for each data point suggests that environmental reconstruction from Isidid internode calcite from sparse data or at time scales less than decades be done with caution. Comparisons within and among colonies do not indicate strong vital effects on ontogenetic variability in the corals, other than possibly close to the central pore of the coral. However, similar Mg/Ca ratios for Isidids from Antarctic and more temperate regions suggest adaptation to local conditions and hence a role for physiology at higher taxonomic levels, at least. Taxonomically higher level vital effects are also suggested by large differences between gorgonian families in their regressions between Mg/Ca and temperature, by Mg/Ca ratios that overlap over a wide temperature and habitat range, and for a non-linear relationship between temperature and the slope of the Mg/Ca-temperature relationship across the order.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0016-7037},
	doi = {10.1016/j.gca.2010.05.024},
	url = {http://dx.doi.org/10.1016/j.gca.2010.05.024},
	author = {Thresher, R E and Wilson, N C and MacRae, C M and Neil, H}
}
@mastersthesis {Waters2010,
	title = {{Temporal and petrogenetic constraints on volcanic accretionary processes at 9-10 degrees north East Pacific Rise}},
	volume = {Ph.D.},
	year = {2010},
	pages = {258},
	school = {Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution},
	type = {phd},
	address = {Cambridge, MA and Woods Hole, MA},
	abstract = {Volcanic accretion at the fast-spreading East Pacific Rise (EPR) occurs over a {\~{}}2-4 km wide neo-volcanic zone on either side of the axial summit trough (AST). Eruption ages are critical for understanding the distribution and timing of volcanic and magmatic activity. Uranium series nuclides are susceptible to fractionation by magmatic processes that occur beneath mid-ocean ridges, and the half-lives of 226Ra (1.6 kyrs) and 230Th (75 kyrs) make them ideally suited for determining eruption ages and placing constraints on eruption frequency and temporal changes in magma chemistry. Accordingly, major and trace element, and long-lived radiogenic and 238U-230Th-226Ra isotope compositions were measured in basalts from 9{\textordmasculine}-10{\textordmasculine}N EPR to determine eruption ages and to place temporal constraints on volcanic and magmatic processes. At 9{\textordmasculine}30{\textquoteright}N EPR, 238U-230Th-226Ra compositions indicate that trace elementally and isotopically enriched mid-ocean ridge basalt (MORB) collected off-axis erupted {\textgreater}8 ka and that E-MORB magmatism is interspersed with normal, depleted MORB magmatism. Lava ages are consistent with eruption from the AST and flow down the ridge flanks, which is in contrast to previous studies that suggested E-MORB erupted from off-axis vents. At 9{\textordmasculine}50{\textquoteright}N EPR, discrete eruptive units are distinguished by high precision 238U, 232Th, and 226Ra sample concentrations, but because the resolution of the 230Th-226Ra model age dating technique is {\~{}}{\textpm}1 kyrs, the surprisingly young ages of these lavas prohibit the construction of an explicit, time-constrained lava stratigraphy. Nonetheless, seven different flows identified within 0.8-2.0 km west of the AST imply greater frequency of flows to these distances than previously recognized. Model age dating of ferrobasalts, basaltic andesites, andesites, and dacites sampled from the east limb of the overlapping spreading center at 9{\textordmasculine}03{\textquoteright}N EPR is difficult due to uncertainties in magma residence times. However, (226Ra/230Th) disequilibria indicate recent basaltic volcanism ({\textless}{\textless}8 ka) up to {\~{}}4 km off-axis. The axial graben at the rise crest sources the most recent volcanic activity and is the dominant location for eruption of high-silica magmas. Major element, trace element, 87Sr/86Sr, and (234U/238U) isotope compositions are consistent with the formation of dacite magmas by extensive crystallization, and 238U-230Th-226Ra systematics imply crustal residence times of {\~{}}8 kyrs.},
	keywords = {AUV ABE (Autonomous Benthic Explorer), HOV Alvin (Human Occupied Vehicle), ROV DSL-120 (Remotely Operated Vehicle), ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1575/1912/3750},
	url = {http://hdl.handle.net/1912/3750},
	author = {Waters, C L}
}
@article {Basak2009,
	title = {{Carbon and oxygen isotope geochemistry of live (stained) benthic foraminifera from the Aleutian Margin and the Southern Australian Margin}},
	journal = {Marine Micropaleontology},
	volume = {70},
	number = {3-4},
	year = {2009},
	pages = {89{\textendash}101},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0377-8398},
	doi = {10.1016/j.marmicro.2008.11.002},
	url = {http://dx.doi.org/10.1016/j.marmicro.2008.11.002},
	author = {Basak, C and Rathburn, A E and Perez, M E and Martin, J B and Kluesner, J W and Levin, L A and De Deckker, P and Gieskes, J M and Abriani, M}
}
@article {Katsioloudis2009,
	title = {{Discovery of the depths: the world{\textquoteright}s oceans have been almost impenetrable to human exploration because of obstacles associated with underwater exploration{\textendash}until the very recent development of remote underwater vehicles}},
	journal = {Technology Teacher},
	volume = {68},
	number = {8},
	year = {2009},
	pages = {12{\textendash}16},
	keywords = {AUV ABE (Autonomous Benthic Explorer), ROV Jason (Remotely Operated Vehicle)},
	issn = {0746-3537},
	author = {Katsioloudis, P J}
}
@article {Podowski2009,
	title = {{Distribution of diffuse flow megafauna in two sites on the Eastern Lau Spreading Center, Tonga}},
	journal = {Deep-Sea Research. Part I: Oceanographic Research Papers},
	volume = {56},
	number = {11},
	year = {2009},
	pages = {2041{\textendash}2056},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0637},
	doi = {10.1016/j.dsr.2009.07.002},
	url = {http://dx.doi.org/10.1016/j.dsr.2009.07.002},
	author = {Podowski, E L and Moore, T S and Zelnio, K A and Luther, G W and Fisher, C R}
}
@article {Sarrazin2009,
	title = {{A dual sensor device to estimate fluid flow velocity at diffuse hydrothermal vents}},
	journal = {Deep-Sea Research. Part I: Oceanographic Research Papers},
	volume = {56},
	number = {11},
	year = {2009},
	pages = {2065{\textendash}2074},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0637},
	doi = {10.1016/j.dsr.2009.06.008},
	url = {http://dx.doi.org/10.1016/j.dsr.2009.06.008},
	author = {Sarrazin, J and Rodier, P and Tivey, M K and Singh, H and Schultz, A and Sarradin, P -M}
}
@article {VanderHam2009,
	title = {{Exploring the utility of an indel-rich, mitochondrial intergenic region as a molecular barcode for bamboo corals (Octocorallia: Isididae)}},
	journal = {Marine Genomics},
	volume = {2},
	number = {3-4},
	year = {2009},
	pages = {183{\textendash}192},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {1874-7787},
	doi = {10.1016/j.margen.2009.10.002},
	url = {http://dx.doi.org/10.1016/j.margen.2009.10.002},
	author = {van der Ham, J L and Brugler, M R and France, S C}
}
@article {Rathburn2009,
	title = {{Geological and biological heterogeneity of the Aleutian Margin (1965{\textendash}4822 m)}},
	journal = {Progress In Oceanography},
	volume = {80},
	number = {1-2},
	year = {2009},
	pages = {22{\textendash}50},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0079-6611},
	doi = {10.1016/j.pocean.2008.12.002},
	url = {http://dx.doi.org/10.1016/j.pocean.2008.12.002},
	author = {Rathburn, A E and Levin, L A and Tryon, M D and Gieskes, J M and Martin, J B and Perez, M E and Fodrie, F J and Neira, C and Fryer, G L and Mendoza, G F and McMillan, P A and Kluesner, J and Adamic, J and Ziebis, W}
}
@article {Petersen2009,
	title = {{The geological setting of the ultramafic-hosted Logatchev hydrothermal field (14 degrees 45'N, Mid-Atlantic Ridge) and its influence on massive sulfide formation}},
	journal = {Lithos},
	volume = {112},
	number = {1-2},
	year = {2009},
	pages = {40{\textendash}56},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0024-4937},
	doi = {10.1016/j.lithos.2009.02.008},
	url = {http://dx.doi.org/10.1016/j.lithos.2009.02.008},
	author = {Petersen, S and Kuhn, K and Kuhn, T and Augustin, N and Hekinian, R and Franz, L and Borowski, C}
}
@article {Becker2009,
	title = {{Importance of seep primary production to Lophelia pertusa and associated fauna in the Gulf of Mexico}},
	journal = {Deep-Sea Research. Part I: Oceanographic Research Papers},
	volume = {56},
	number = {5},
	year = {2009},
	pages = {786{\textendash}800},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0637},
	doi = {10.1016/j.dsr.2008.12.006},
	url = {http://dx.doi.org/10.1016/j.dsr.2008.12.006},
	author = {Becker, E L and Cordes, E E and Macko, S A and Fisher, C R}
}
@article {Nevala2009,
	title = {{Jason encounters the carnivorous sea squirt}},
	journal = {Oceanus},
	volume = {47},
	number = {2},
	year = {2009},
	pages = {7},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0029-8182},
	url = {http://www.whoi.edu/oceanus/viewArticle.do?id=56367{\&}sectionid=1021},
	author = {Nevala, A E and Lippsett, L}
}
@article {White2009,
	title = {{Laser Raman spectroscopy as a technique for identification of seafloor hydrothermal and cold seep minerals}},
	journal = {Chemical Geology},
	volume = {259},
	number = {3-4},
	year = {2009},
	pages = {240{\textendash}252},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0009-2541},
	doi = {10.1016/j.chemgeo.2008.11.008},
	url = {http://dx.doi.org/10.1016/j.chemgeo.2008.11.008},
	author = {White, S N}
}
@book {Abrams2009,
	title = {{The N456 Navigator system}},
	series = {WHOI technical reports},
	volume = {2009-01},
	year = {2009},
	pages = {52},
	publisher = {Woods Hole Oceanographic Institution},
	organization = {Woods Hole Oceanographic Institution},
	address = {Woods Hole, MA},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1575/1912/2717},
	url = {http://hdl.handle.net/1912/2717},
	author = {Abrams, L J}
}
@article {Glazer2009,
	title = {{Redox speciation and distribution within diverse iron-dominated microbial habitats at Loihi Seamount}},
	journal = {Geomicrobiology Journal},
	volume = {26},
	number = {8},
	year = {2009},
	pages = {606{\textendash}622},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0149-0451},
	doi = {10.1080/01490450903263392},
	url = {http://dx.doi.org/10.1080/01490450903263392},
	author = {Glazer, B T and Rouxel, O J}
}
@article {White2009a,
	title = {{Significance of widespread low effusion rate eruptions over the past two million years for delivery of magma to the overlapping spreading centers at 9 degrees N East Pacific Rise}},
	journal = {Earth and Planetary Science Letters},
	volume = {280},
	number = {1-4},
	year = {2009},
	pages = {175{\textendash}184},
	keywords = {ROV DSL-120 (Remotely Operated Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0012-821X},
	doi = {10.1016/j.epsl.2009.01.030},
	url = {http://dx.doi.org/10.1016/j.epsl.2009.01.030},
	author = {White, S M and Mason, J L and Macdonald, K C and Perfit, M R and Wanless, V D and Klein, E M}
}
@article {Tunnicliffe2009,
	title = {{Survival of mussels in extremely acidic waters on a submarine volcano}},
	journal = {Nature Geoscience},
	volume = {2},
	number = {5},
	year = {2009},
	month = {may},
	pages = {344{\textendash}348},
	publisher = {NATURE PUBLISHING GROUP},
	address = {NEW YORK; 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1752-0894},
	doi = {10.1038/NGEO500},
	author = {Tunnicliffe, V and Davies, K T A and Butterfield, D A and Embley, R W and Rose, J M and Chadwick, W W}
}
@article {Breier2009,
	title = {{A suspended-particle rosette multi-sampler for discrete biogeochemical sampling in low-particle-density waters}},
	journal = {Deep-Sea Research. Part II: Topical Studies in Oceanography},
	volume = {56},
	number = {9},
	year = {2009},
	pages = {1579{\textendash}1589},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0645},
	doi = {10.1016/j.dsr.2009.04.005},
	url = {http://dx.doi.org/10.1016/j.dsr.2009.04.005},
	author = {Breier, J A and Rauch, C G and McCartney, K and Toner, B M and Fakra, S C and White, S N and German, C R}
}
@article {Nooner2009,
	title = {{Volcanic inflation measured in the caldera of Axial Seamount: Implications for magma supply and future eruptions}},
	journal = {Geochemistry, Geophysics, Geosystems},
	volume = {10},
	year = {2009},
	pages = {Q02002},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1525-2027},
	doi = {10.1029/2008GC002315},
	url = {http://dx.doi.org/10.1029/2008GC002315},
	author = {Nooner, S L and Chadwick, W W}
}
@article {Clague2009,
	title = {{Widespread strombolian eruptions of mid-ocean ridge basalt}},
	journal = {Journal of Volcanology and Geothermal Research},
	volume = {180},
	number = {2-4},
	year = {2009},
	pages = {171{\textendash}188},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0377-0273},
	doi = {10.1016/j.jvolgeores.2008.08.007},
	url = {http://dx.doi.org/10.1016/j.jvolgeores.2008.08.007},
	author = {Clague, D A and Paduan, J B and Davis, A S}
}
@article {Won2008,
	title = {{Absence of cospeciation between deep-sea mytilids and their thiotrophic endosymbionts}},
	journal = {Journal of Shellfish Research},
	volume = {27},
	number = {1},
	year = {2008},
	pages = {129{\textendash}138},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0730-8000},
	doi = {10.2983/0730-8000(2008)27[129:AOCBDM]2.0.CO;2},
	url = {http://dx.doi.org/10.2983/0730-8000(2008)27[129:AOCBDM]2.0.CO 2},
	author = {Won, Y -J and Jones, W J and Vrijenhoek, R C}
}
@article {Wheat2008,
	title = {{Borehole observations of fluid flow from South Chamorro Seamount, an active serpentinite mud volcano in the Mariana forearc}},
	journal = {Earth and Planetary Science Letters},
	volume = {267},
	number = {3-4},
	year = {2008},
	pages = {401{\textendash}409},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0012-821X},
	doi = {10.1016/j.epsl.2007.11.057},
	url = {http://dx.doi.org/10.1016/j.epsl.2007.11.057},
	author = {Wheat, C G and Fryer, P and Fisher, A T and Hulme, S and Jannasch, H W and Mottl, M J}
}
@article {Logan2008,
	title = {{Comparative ultrastructure of digestive diverticulae in bathymodiolin mussels: Discovery of an unknown spherical inclusion (SIX) in digestive cells of a seep mussel}},
	journal = {Journal of Shellfish Research},
	volume = {27},
	number = {1},
	year = {2008},
	pages = {97{\textendash}105},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0730-8000},
	doi = {10.2983/0730-8000(2008)27[97:CUODDI]2.0.CO;2},
	url = {http://dx.doi.org/10.2983/0730-8000(2008)27[97:CUODDI]2.0.CO 2},
	author = {Logan, C R and Evans, M T and Ward, M E and Scott, J L and Carnegie, R B and Van Dover, C L}
}
@article {Chadwick2008,
	title = {{Direct video and hydrophone observations of submarine explosive eruptions at NW Rota-1 volcano, Mariana arc}},
	journal = {Journal of Geophysical Research},
	volume = {113},
	year = {2008},
	pages = {B08S10},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0148-0227},
	doi = {10.1029/2007JB005215},
	url = {http://dx.doi.org/10.1029/2007JB005215},
	author = {Chadwick, W W and Cashman, K V and Embley, R W and Matsumoto, H and Dziak, R P and de Ronde, C E J and Lau, T K and Deardorff, N D and Merle, S G}
}
@article {Walker2008,
	title = {{Dissolved inorganic carbon isotopic composition of low-temperature axial and ridge-flank hydrothermal fluids of the Juan de Fuca Ridge}},
	journal = {Marine Chemistry},
	volume = {108},
	number = {1-2},
	year = {2008},
	pages = {123{\textendash}136},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0304-4203},
	doi = {10.1016/j.marchem.2007.11.002},
	url = {http://dx.doi.org/10.1016/j.marchem.2007.11.002},
	author = {Walker, B D and McCarthy, M D and Fisher, A T and Guilderson, T P}
}
@article {Johnson2008,
	title = {{DNA barcoding of Lepetodrilus limpets reveals cryptic species}},
	journal = {Journal of Shellfish Research},
	volume = {27},
	number = {1},
	year = {2008},
	pages = {43{\textendash}51},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0730-8000},
	doi = {10.2983/0730-8000(2008)27[43:DBOLLR]2.0.CO;2},
	url = {http://dx.doi.org/10.2983/0730-8000(2008)27[43:DBOLLR]2.0.CO 2},
	author = {Johnson, S B and Waren, A and Vrijenhoek, R C}
}
@article {Walker2008a,
	title = {{Eruption-fed particle plumes and volcaniclastic deposits at a submarine volcano: NW Rota-1, Mariana Arc}},
	journal = {Journal of Geophysical Research},
	volume = {113},
	year = {2008},
	pages = {B08S11},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0148-0227},
	doi = {10.1029/2007JB005441},
	url = {http://dx.doi.org/10.1029/2007JB005441},
	author = {Walker, S L and Baker, E T and Resing, J A and Chadwick, W W and Lebon, G T and Lupton, J E and Merle, S G}
}
@article {Hsu-Kim2008,
	title = {{Formation of Zn- and Fe-sulfides near hydrothermal vents at the Eastern Lau Spreading Center: implications for sulfide bioavailability to chemoautotrophs}},
	journal = {Geochemical Transactions},
	volume = {9},
	number = {6},
	year = {2008},
	pages = {6},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1467-4866},
	doi = {10.1186/1467-4866-9-6},
	url = {http://dx.doi.org/10.1186/1467-4866-9-6},
	author = {Hsu-Kim, H and Mullaugh, K M and Tsang, J J and Yucel, M and Luther, G W}
}
@article {Henry2008,
	title = {{Metabolic rates and thermal tolerances of chemoautotrophic symbioses from Lau Basin hydrothermal vents and their implications for species distributions}},
	journal = {Deep-Sea Research. Part I: Oceanographic Research Papers},
	volume = {55},
	number = {5},
	year = {2008},
	pages = {679{\textendash}695},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0637},
	doi = {10.1016/j.dsr.2008.02.001},
	url = {http://dx.doi.org/10.1016/j.dsr.2008.02.001},
	author = {Henry, M S and Childress, J J and Figueroa, D}
}
@article {Waite2008,
	title = {{Variation in sulfur speciation with shellfish presence at a Lau Basin diffuse flow vent site}},
	journal = {Journal of Shellfish Research},
	volume = {27},
	number = {1},
	year = {2008},
	pages = {163{\textendash}168},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0730-8000},
	doi = {10.2983/0730-8000(2008)27[163:VISSWS]2.0.CO;2},
	url = {http://dx.doi.org/10.2983/0730-8000(2008)27[163:VISSWS]2.0.CO 2},
	author = {Waite, T J and Moore, T S and Childress, J J and Hsu-Kim, H and Mullaugh, K M and Nuzzio, D B and Paschal, A N and Tsang, J and Fisher, C R and Luther, G W}
}
@inbook {Humphris2008,
	title = {{Vehicles for deep-sea exploration}},
	booktitle = {Encyclopedia of ocean sciences},
	volume = {6},
	year = {2008},
	pages = {4647{\textendash}4659},
	publisher = {Academic Press},
	organization = {Academic Press},
	address = {San Diego, CA},
	keywords = {AUV ABE (Autonomous Benthic Explorer), AUV Sentry (Autonomous Underwater Vehicle), HOV Alvin (Human Occupied Vehicle), ROV DSL-120 (Remotely Operated Vehicle), ROV Jason (Remotely Operated Vehicle)},
	isbn = {9780123744739},
	doi = {10.1016/B978-012374473-9.00734-7},
	url = {http://dx.doi.org/10.1016/B978-012374473-9.00734-7},
	author = {Humphris, S E},
	editor = {Steele, J H}
}
@article {Chen2008,
	title = {{Vision-based tracking with projective mapping for parameter identification of remotely operated vehicles}},
	journal = {Ocean Engineering},
	volume = {35},
	number = {10},
	year = {2008},
	pages = {983{\textendash}994},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0029-8018},
	doi = {10.1016/j.oceaneng.2008.03.001},
	url = {http://dx.doi.org/10.1016/j.oceaneng.2008.03.001},
	author = {Chen, H -H}
}
@article {Mullaugh2008,
	title = {{Voltammetric (micro)electrodes for the in situ study of Fe2+ oxidation kinetics in hot springs and S2O3-2- production at hydrothermal vents}},
	journal = {Electroanalysis},
	volume = {20},
	number = {3},
	year = {2008},
	pages = {280{\textendash}290},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1040-0397},
	doi = {10.1002/elan.200704056},
	url = {http://dx.doi.org/10.1002/elan.200704056},
	author = {Mullaugh, K M and Luther, G W and Ma, S and Moore, T S and Yucel, M and Becker, E L and Podowski, E L and Fisher, C R and Trouwborst, R E and Pierson, B K}
}
@article {Garcia2008,
	title = {{Widespread secondary volcanism near northern Hawaiian Islands}},
	journal = {EOS, Transactions, American Geophysical Union},
	volume = {89},
	number = {52},
	year = {2008},
	pages = {542{\textendash}543},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0096-3941},
	doi = {10.1029/2008EO520002},
	url = {http://dx.doi.org/10.1029/2008EO520002},
	author = {Garcia, M O and Ito, G and Weis, D and Geist, D J and Swinnard, L and Bianco, T and Flinders, A and Taylor, B and Appelgate, B and Blay, C and Hanano, D and Silva, I N and Naumann, T and Maerschalk, C and Harpp, K S and Christensen, B and Sciaroni, L and Tagami, T and Yamasaki, S}
}
@inbook {Singh2007,
	title = {{Advances in High Resolution Imaging from Underwater Vehicles}},
	booktitle = {Robotics Research: Results of the 12th International Symposium ISRR},
	series = {Springer Tracts in Advanced Robotics},
	number = {28},
	year = {2007},
	pages = {430{\textendash}448},
	publisher = {Springer},
	organization = {Springer},
	address = {Berlin},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	isbn = {3-540-48110-9},
	doi = {10.1007/978-3-540-48113-3_37},
	url = {http://dx.doi.org/10.1007/978-3-540-48113-3{\_}37},
	author = {Singh, H and Roman, C and Pizarro, O and Eustice, R}
}
@article {Yoerger2007b,
	title = {{Autonomous and remotely operated vehicle technology for hydrothermal vent discovery, exploration, and sampling}},
	journal = {Oceanography},
	volume = {20},
	number = {1},
	year = {2007},
	pages = {152{\textendash}161},
	keywords = {AUV ABE (Autonomous Benthic Explorer), ROV Jason (Remotely Operated Vehicle), ROV Medea (Remotely Operated Vehicle)},
	issn = {1042-8275},
	doi = {10.5670/oceanog.2007.89},
	author = {Yoerger, D R and Bradley, A M and Jakuba, M V and German, C R and Shank, T M and Tivey, M}
}
@book {Roberts2007,
	title = {{Expedition to the Deep Slope 2007, June 4 - July 6, 2007}},
	volume = {2008},
	year = {2007},
	publisher = {U.S. Dept. of Commerce, National Oceanic and Atmospheric Administration, Office of Ocean Exploration},
	organization = {U.S. Dept. of Commerce, National Oceanic and Atmospheric Administration, Office of Ocean Exploration},
	address = {Washington, D.C.},
	keywords = {ROV Jason (Remotely Operated Vehicle), ROV Medea (Remotely Operated Vehicle)},
	url = {http://oceanexplorer.noaa.gov/explorations/07mexico/},
	author = {Roberts, H H and Potter, J M}
}
@article {Voight2007,
	title = {{Experimental deep-sea deployments reveal diverse Northeast Pacific wood-boring bivalves of Xylophagainae (Myoida: Pholadidae)}},
	journal = {Journal of Molluscan Studies},
	volume = {73},
	number = {4},
	year = {2007},
	pages = {377{\textendash}391},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0260-1230},
	doi = {10.1093/mollus/eym034},
	url = {http://dx.doi.org/10.1093/mollus/eym034},
	author = {Voight, J R}
}
@article {Embley2007,
	title = {{Exploring the submarine ring of fire: Mariana Arc-Western Pacific}},
	journal = {Oceanography},
	volume = {20},
	number = {4},
	year = {2007},
	pages = {69{\textendash}80},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {1042-8275},
	doi = {10.5670/oceanog.2007.07},
	author = {Embley, R W and Baker, E T and Butterfield, D A and Chadwick, W W and Lupton, J E and Resing, J A and de Ronde, C E J and Nakamura, K I and Tunnicliffe, V and Dower, J F and Merle, S G}
}
@article {VanDover2007a,
	title = {{A fungal epizootic in mussels at a deep-sea hydrothermal vent}},
	journal = {Marine Ecology},
	volume = {28},
	number = {1},
	year = {2007},
	pages = {54{\textendash}62},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0173-9565},
	doi = {10.1111/j.1439-0485.2006.00121.x},
	url = {http://dx.doi.org/10.1111/j.1439-0485.2006.00121.x},
	author = {Van Dover, C L and Ward, M E and Scott, J L and Underdown, J and Anderson, B and Gustafson, C and Whalen, M and Carnegie, R B}
}
@article {Glickson2007,
	title = {{Geology and hydrothermal evolution of the Mothra Hydrothermal Field, Endeavour Segment, Juan de Fuca Ridge}},
	journal = {Geochemistry, Geophysics, Geosystems},
	volume = {8},
	year = {2007},
	pages = {Q06010},
	keywords = {AUV ABE (Autonomous Benthic Explorer), HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {1525-2027},
	doi = {10.1029/2007GC001588},
	url = {http://dx.doi.org/10.1029/2007GC001588},
	author = {Glickson, D A and Kelley, D S and Delaney, J R}
}
@article {Jones2007,
	title = {{Molecular phylogeny of the East Pacific squat lobsters of the genus Munidopsis (decapoda: galatheidae) with the descriptions of seven new species}},
	journal = {Journal of Crustacean Biology},
	volume = {27},
	number = {3},
	year = {2007},
	pages = {477{\textendash}501},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0278-0372},
	doi = {10.1651/S-2791.1},
	url = {http://dx.doi.org/10.1651/S-2791.1},
	author = {Jones, W J and Macpherson, E}
}
@article {Shields2007,
	title = {{New nemertean worms (Carcinonemertidae) on bythograeid crabs (decapoda: brachyura) from Pacific hydrothermal vent sites}},
	journal = {Journal of Crustacean Biology},
	volume = {27},
	number = {4},
	year = {2007},
	pages = {681{\textendash}692},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0278-0372},
	doi = {10.1651/S-2794.1},
	url = {http://dx.doi.org/10.1651/S-2794.1},
	author = {Shields, J D and Segonzac, M}
}
@article {Nishi2007,
	title = {{A new species of Phyllochaetopterus (Chaetopteridae : Annelida) from near hydrothermal vents in the Lau Basin, western Pacific Ocean}},
	journal = {Zootaxa},
	number = {1621},
	year = {2007},
	pages = {55{\textendash}64},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1175-5326},
	url = {http://www.mapress.com/zootaxa/2007f/z01621p064f.pdf},
	author = {Nishi, E and Rouse, G W}
}
@article {Perk2007,
	title = {{Petrology and geochemistry of primitive lower oceanic crust from Pito Deep: Implications for the accretion of the lower crust at the Southern East Pacific Rise}},
	journal = {Contributions to Mineralogy and Petrology},
	volume = {154},
	number = {5},
	year = {2007},
	pages = {575{\textendash}590},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0010-7999},
	doi = {10.1007/s00410-007-0210-z},
	url = {http://dx.doi.org/10.1007/s00410-007-0210-z},
	author = {Perk, N W and Coogan, L A and Karson, J A and Klein, E M and Hanna, H D}
}
@article {Sharma2007,
	title = {{Search for the proverbial mantle osmium sources to the oceans: Hydrothermal alteration of mid-ocean ridge basalt}},
	journal = {Geochimica et Cosmochimica Acta},
	volume = {71},
	number = {19},
	year = {2007},
	pages = {4655{\textendash}4667},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0016-7037},
	doi = {10.1016/j.gca.2007.06.062},
	url = {http://dx.doi.org/10.1016/j.gca.2007.06.062},
	author = {Sharma, M and Rosenberg, E J and Butterfield, D A}
}
@article {Roman2007,
	title = {{A self-consistent bathymetric mapping algorithm}},
	journal = {Journal of Field Robotics},
	volume = {24},
	number = {1-2},
	year = {2007},
	pages = {23{\textendash}50},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1556-4959},
	doi = {10.1002/rob.20164},
	url = {http://dx.doi.org/10.1002/rob.20164},
	author = {Roman, C and Singh, H}
}
@article {Sohn2007,
	title = {{Stochastic analysis of exit fluid temperature records from the active TAG hydrothermal mound (Mid-Atlantic Ridge, 26 degrees N); 1, Modes of variability and implications for subsurface flow}},
	journal = {Journal of Geophysical Research},
	volume = {112},
	number = {B7},
	year = {2007},
	pages = {B07101},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0148-0227},
	doi = {10.1029/2006JB004435},
	url = {http://dx.doi.org/10.1029/2006JB004435},
	author = {Sohn, R A}
}
@article {Singh2007a,
	title = {{Towards high-resolution imaging from underwater vehicles}},
	journal = {International Journal of Robotics Research},
	volume = {26},
	number = {1},
	year = {2007},
	pages = {55{\textendash}74},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0278-3649},
	doi = {10.1007/978-3-540-48113-3_37},
	url = {http://dx.doi.org/10.1007/978-3-540-48113-3{\_}37},
	author = {Singh, H and Roman, C and Pizarro, O and Eustice, R and Can, A}
}
@article {Nevala2007,
	title = {{Undersea eruption traps devices on the bottom of the Pacific}},
	journal = {Oceanus},
	volume = {45},
	number = {3},
	year = {2007},
	pages = {6},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0029-8182},
	url = {http://www.whoi.edu/oceanus/viewArticle.do?id=27286{\&}archives=true},
	author = {Nevala, A E}
}
@article {Lang2006,
	title = {{Dissolved organic carbon in ridge-axis and ridge-flank hydrothermal systems}},
	journal = {Geochimica et Cosmochimica Acta},
	volume = {70},
	number = {15},
	year = {2006},
	pages = {3830{\textendash}3842},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0016-7037},
	doi = {10.1016/j.gca.2006.04.031},
	url = {http://dx.doi.org/10.1016/j.gca.2006.04.031},
	author = {Lang, S Q and Butterfield, D A and Lilley, M D and Johnson, H P and Hedges, J I}
}
@article {Rona2006,
	title = {{Entrainment and bending in a major hydrothermal plume, Main Endeavour Field, Juan de Fuca Ridge}},
	journal = {Geophysical Research Letters},
	volume = {33},
	number = {19},
	year = {2006},
	pages = {L19313},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0094-8276},
	doi = {10.1029/2006gl027211},
	url = {http://dx.doi.org/10.1029/2006gl027211},
	author = {Rona, P A and Bemis, K G and Jones, C D and Jackson, D R and Mitsuzawa, K and Silver, D}
}
@article {Gallant2006,
	title = {{Geochemical controls on hydrothermal fluids from the Kairei and Edmond Vent Fields, 23 degrees-25 degrees S, Central Indian Ridge}},
	journal = {Geochemistry, Geophysics, Geosystems},
	volume = {7},
	year = {2006},
	pages = {Q06018},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1525-2027},
	doi = {10.1029/2005gc001067},
	url = {http://dx.doi.org/10.1029/2005gc001067},
	author = {Gallant, R M and Von Damm, K L}
}
@article {Kristall2006,
	title = {{Growth history of a diffusely venting sulfide structure from the Juan de Fuca Ridge: A petrological and geochemical study}},
	journal = {Geochemistry, Geophysics, Geosystems},
	volume = {7},
	year = {2006},
	pages = {Q07001},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1525-2027},
	doi = {10.1029/2005GC001166},
	url = {http://dx.doi.org/10.1029/2005GC001166},
	author = {Kristall, B and Kelley, D S and Hannington, M D and Delaney, J R}
}
@article {Bingham2006a,
	title = {{Integrating precision relative positioning into Jason/Medea ROV operations}},
	journal = {Marine Technology Society Journal},
	volume = {40},
	number = {1},
	year = {2006},
	pages = {87{\textendash}96},
	keywords = {ROV Jason (Remotely Operated Vehicle), ROV Medea (Remotely Operated Vehicle)},
	issn = {0025-3324},
	doi = {10.4031/002533206787353664},
	url = {http://dx.doi.org/10.4031/002533206787353664},
	author = {Bingham, B and Mindell, D and Wilcox, T and Bowen, A}
}
@article {Nevala2006,
	title = {{Jason versus the volcano}},
	journal = {Oceanus},
	volume = {45},
	number = {2},
	year = {2006},
	pages = {4{\textendash}5},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0029-8182},
	url = {http://www.whoi.edu/oceanus/viewArticle.do?id=14507{\&}archives=true},
	author = {Nevala, A E}
}
@article {Proskurowski2006,
	title = {{Low temperature volatile production at the Lost City Hydrothermal Field: evidence from a hydrogen stable isotope geothermometer}},
	journal = {Chemical Geology},
	volume = {229},
	number = {4},
	year = {2006},
	pages = {331{\textendash}343},
	keywords = {AUV ABE (Autonomous Benthic Explorer), HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0009-2541},
	doi = {10.1016/j.chemgeo.2005.11.005},
	url = {http://dx.doi.org/10.1016/j.chemgeo.2005.11.005},
	author = {Proskurowski, G and Lilley, M D and Kelley, D S and Olson, E J}
}
@article {Huber2006,
	title = {{Microbial life in ridge flank crustal fluids}},
	journal = {Environmental Microbiology},
	volume = {8},
	number = {1},
	year = {2006},
	pages = {88{\textendash}99},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1462-2912},
	doi = {10.1111/j.1462-2920.2005.00872.x},
	url = {http://dx.doi.org/10.1111/j.1462-2920.2005.00872.x},
	author = {Huber, J A and Johnson, H P and Butterfield, D A and Baross, J A}
}
@article {Lehnert2006,
	title = {{New species of deep-sea demosponges (Porifera) from the Aleutian Islands (Alaska, USA)}},
	journal = {Zootaxa},
	number = {1250},
	year = {2006},
	pages = {1{\textendash}35},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1175-5326},
	url = {http://www.mapress.com/zootaxa/2006f/z01250p035f.pdf},
	author = {Lehnert, H and Stone, R and Heimler, W}
}
@book {Embley2006a,
	title = {{NOAA Ocean Explorer: Submarine ring of fire 2006}},
	volume = {2007},
	year = {2006},
	publisher = {U.S. Dept. of Commerce, National Oceanic and Atmospheric Administration, Office of Ocean Exploration},
	organization = {U.S. Dept. of Commerce, National Oceanic and Atmospheric Administration, Office of Ocean Exploration},
	address = {Silver Spring, Md.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	url = {http://oceanexplorer.noaa.gov/explorations/06fire/welcome.html},
	author = {Embley, R W and Gottfried, S}
}
@article {Levin2006,
	title = {{Recruitment response of methane-seep macrofauna to sulfide-rich sediments: An in situ experiment}},
	journal = {Journal of Experimental Marine Biology and Ecology},
	volume = {330},
	number = {1},
	year = {2006},
	pages = {132{\textendash}150},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0022-0981},
	doi = {10.1016/j.jembe.2005.12.022},
	url = {http://dx.doi.org/10.1016/j.jembe.2005.12.022},
	author = {Levin, L A and Ziebis, W and Mendoza, G F and Growney-Cannon, V and Walther, S}
}
@article {Johnson2006,
	title = {{On sampling the upper crustal reservoir of the NE Pacific Ocean}},
	journal = {Geofluids},
	volume = {6},
	number = {3},
	year = {2006},
	pages = {251{\textendash}271},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1468-8123},
	doi = {10.1111/j.1468-8123.2006.00151.x},
	url = {http://dx.doi.org/10.1111/j.1468-8123.2006.00151.x},
	author = {Johnson, H P and Baross, J A and Bjorklund, T A}
}
@article {Wanless2006,
	title = {{Submarine radial vents on Mauna Loa Volcano, Hawai{\textquoteright}i}},
	journal = {Geochemistry, Geophysics, Geosystems},
	volume = {7},
	year = {2006},
	pages = {Q05001},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1525-2027},
	doi = {10.1029/2005gc001086},
	url = {http://dx.doi.org/10.1029/2005gc001086},
	author = {Wanless, V D and Garcia, M O and Trusdell, F A and Rhodes, J M and Norman, M D and Weis, D and Fornari, D J and Kurz, M D and Guillou, H}
}
@article {Larsen2006,
	title = {{Tanaidacea (Crustacea; Peracarida) from chemically reduced habitats - the hydrothermal vent system of the Juan de Fuca Ridge, Escabana Trough and Gorda Ridge, northeast Pacific}},
	journal = {Zootaxa},
	number = {1164},
	year = {2006},
	pages = {1{\textendash}33},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {1175-5326},
	url = {http://www.mapress.com/zootaxa/2006/zt01164p033.pdf},
	author = {Larsen, K}
}
@article {Reysenbach2006,
	title = {{A ubiquitous thermoacidophilic archaeon from deep-sea hydrothermal vents}},
	journal = {Nature},
	volume = {442},
	number = {7101},
	year = {2006},
	pages = {444{\textendash}447},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0028-0836},
	doi = {10.1038/nature04921},
	url = {http://dx.doi.org/10.1038/nature04921},
	author = {Reysenbach, A -L and Liu, Y T and Banta, A B and Beveridge, T J and Kirshtein, J D and Schouten, S and Tivey, M K and Von Damm, K L and Voytek, M A}
}
@article {Fryer2006,
	title = {{Variability in serpentinite mudflow mechanisms and sources: ODP drilling results on Mariana forearc seamounts}},
	journal = {Geochemistry, Geophysics, Geosystems},
	volume = {7},
	year = {2006},
	pages = {Q08014},
	keywords = {ROV DSL-120 (Remotely Operated Vehicle), ROV Jason (Remotely Operated Vehicle), ROV Medea (Remotely Operated Vehicle)},
	issn = {1525-2027},
	doi = {10.1029/2005GC001201},
	url = {http://dx.doi.org/10.1029/2005GC001201},
	author = {Fryer, P and Gharib, J and Ross, K and Savov, I and Mottl, M J}
}
@article {Pearson2005,
	title = {{Bacterial incorporation of relict carbon in the hydrothermal environment of Guaymas Basin}},
	journal = {Geochimica et Cosmochimica Acta},
	volume = {69},
	number = {23},
	year = {2005},
	pages = {5477{\textendash}5486},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0016-7037},
	doi = {10.1016/j.gca.2005.07.007},
	url = {http://dx.doi.org/10.1016/j.gca.2005.07.007},
	author = {Pearson, A and Seewald, J S and Eglinton, T I}
}
@mastersthesis {Gier2005,
	title = {{The geological implications of the basalts and sediments of the Lucky Strike segment}},
	volume = {Ph.D.},
	year = {2005},
	pages = {244},
	school = {Columbia University},
	type = {phd},
	address = {New York, N.Y.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	url = {http://digitalcommons.libraries.columbia.edu/dissertations/AAI3188739},
	author = {Gier, E J}
}
@mastersthesis {Eustice2005,
	title = {{Large-area visually augmented navigation for autonomous underwater vehicles}},
	volume = {Ph.D.},
	year = {2005},
	pages = {187},
	school = {Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution},
	type = {phd},
	address = {Cambridge, Mass. and Woods Hole, Mass.},
	keywords = {AUV ABE (Autonomous Benthic Explorer), HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1575/1912/1414},
	url = {http://hdl.handle.net/1912/1414},
	author = {Eustice, R M}
}
@article {Karson2005,
	title = {{Nested-scale investigation of tectonic windows into super-fast spread crust exposed at the Pito Deep Rift}},
	journal = {InterRidge Newsletter},
	volume = {14},
	year = {2005},
	pages = {5{\textendash}8},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV DSL-120 (Remotely Operated Vehicle), ROV Jason (Remotely Operated Vehicle)},
	url = {http://www.interridge.org/files/interridge/IR{\_}news{\_}14.pdf},
	author = {Karson, J A and Francheteau, J and Gee, J S and Gillis, K M and Hayman, N W and Hekinian, R and Hey, R N and Hurst, S D and Klein, E M and Naar, D P and Varga, R J and Party, Pito Deep 2005 Scientific}
}
@mastersthesis {Roman2005,
	title = {{Self consistent bathymetric mapping from robotic vehicles in the deep ocean}},
	volume = {Ph.D.},
	year = {2005},
	pages = {129},
	school = {Massachusetts Institute of Technology and Woods Hole Oceanographic Institution},
	type = {phd},
	address = {Cambridge, MA and Woods Hole, MA},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1575/1912/1572},
	url = {http://hdl.handle.net/1912/1572},
	author = {Roman, C N}
}
@article {McKiness2005a,
	title = {{The ubiquitous mussel: Bathymodiolus aff. brevior symbiosis at the Central Indian Ridge hydrothermal vents}},
	journal = {Marine Ecology Progress Series},
	volume = {295},
	year = {2005},
	pages = {183{\textendash}190},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0171-8630},
	doi = {10.3354/meps295183},
	url = {http://dx.doi.org/10.3354/meps295183},
	author = {McKiness, Z P and Cavanaugh, C M}
}
@mastersthesis {Wichers2005,
	title = {{Verification of numerical models for hydrothermal plume water through field measurements at TAG}},
	volume = {M.S.},
	year = {2005},
	pages = {75},
	school = {Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution},
	type = {phd},
	address = {Cambridge, Mass. and Woods Hole, Mass.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1575/1912/1640},
	url = {http://hdl.handle.net/1912/1640},
	author = {Wichers, S}
}
@article {Mottl2004,
	title = {{Chemistry of springs across the Mariana forearc shows progressive devolatilization of the subducting plate}},
	journal = {Geochimica et Cosmochimica Acta},
	volume = {68},
	number = {23},
	year = {2004},
	pages = {4915{\textendash}4933},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0016-7037},
	doi = {10.1016/j.gca.2004.05.037},
	url = {http://dx.doi.org/10.1016/j.gca.2004.05.037},
	author = {Mottl, M A and Wheat, C G and Fryer, P and Gharib, J and Martin, J B}
}
@article {Gallager2004,
	title = {{Contribution of fine-scale vertical structure and swimming behavior to formation of plankton layers on Georges Bank}},
	journal = {Marine Ecology Progress Series},
	volume = {267},
	year = {2004},
	pages = {27{\textendash}43},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0170-8630},
	doi = {10.3354/meps267027},
	url = {http://dx.doi.org/10.3354/meps267027},
	author = {Gallager, S M and Yamazaki, H and Davis, C S}
}
@book {Mathez2004,
	title = {{The earth machine: The science of a dynamic planet}},
	year = {2004},
	pages = {378},
	publisher = {Columbia University Press},
	organization = {Columbia University Press},
	address = {New York, N.Y.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	isbn = {023112578X},
	author = {Mathez, E A and Webster, J D}
}
@article {Goffredi2004a,
	title = {{Novel forms of structural integration between microbes and a hydrothermal vent gastropod from the Indian Ocean}},
	journal = {Applied and Environmental Microbiology},
	volume = {70},
	number = {5},
	year = {2004},
	pages = {3082{\textendash}3090},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0099-2240},
	doi = {10.1128/AEM.70.5.3082-3090.2004},
	url = {http://dx.doi.org/10.1128/AEM.70.5.3082-3090.2004},
	author = {Goffredi, S K and Waren, A and Orphan, V J and Van Dover, C L and Vrijenhoek, R C}
}
@article {Sheader2004,
	title = {{Reproductive Ecology of Bouvierella curtirama (Amphipoda:Eusiridae) from chemically distinct vents in the Lucky Strike vent field, Mid-Atlantic Ridge}},
	journal = {Marine Biology},
	volume = {144},
	number = {3},
	year = {2004},
	pages = {503{\textendash}514},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0025-3162},
	doi = {10.1007/s00227-003-1211-8},
	url = {http://dx.doi.org/10.1007/s00227-003-1211-8},
	author = {Sheader, M and Van Dover, C L and Thurston, M H}
}
@article {Waren2003,
	title = {{A hot-vent gastropod with iron-sulphide dermal sclerites}},
	journal = {Science},
	volume = {302},
	number = {5647},
	year = {2003},
	pages = {1007},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0036-8075},
	doi = {10.1126/science.1087696},
	url = {http://dx.doi.org/10.1126/science.1087696},
	author = {Waren, A and Bengtson, S and Goffredi, S K and Van Dover, C L}
}
@article {Dziak2003,
	title = {{Hydrothermal temperature changes at the southern Juan de Fuca Ridge associated with MW 6.2 Blanco Transform earthquake}},
	journal = {Geology},
	volume = {31},
	number = {2},
	year = {2003},
	pages = {119{\textendash}122},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0091-7613},
	doi = {10.1130/0091-7613(2003)031<0119:HTCATS>2.0.CO;2},
	url = {http://dx.doi.org/10.1130/0091-7613(2003)031{\%}3C0119:HTCATS{\%}3E2.0.CO 2},
	author = {Dziak, R P and Chadwick, W W and Fox, C G and Embley, R W}
}
@article {Schrenk2003,
	title = {{Incidence and diversity of mcroorganisms within the walls of an active deep-sea sulfide chimney}},
	journal = {Applied and Environmental Microbiology},
	volume = {69},
	number = {6},
	year = {2003},
	pages = {3580{\textendash}3592},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0099-2240},
	doi = {10.1128/AEM.69.6.3580-3592.2003},
	url = {http://dx.doi.org/10.1128/AEM.69.6.3580-3592.2003},
	author = {Schrenk, M O and Kelley, D S and Delaney, J R and Baross, J A}
}
@article {Lilley2003,
	title = {{Magmatic events can produce rapid changes in hydrothermal vent chemistry}},
	journal = {Nature},
	volume = {422},
	number = {6934},
	year = {2003},
	pages = {878{\textendash}881},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0028-0836},
	doi = {10.1038/nature01569},
	url = {http://dx.doi.org/10.1038/nature01569},
	author = {Lilley, M D and Butterfield, D A and Lupton, J E and Olson, E J}
}
@inbook {Board2003a,
	title = {{Ocean research technologies}},
	booktitle = {Exploration of the Seas: Voyage into the Unknown},
	year = {2003},
	pages = {97{\textendash}127},
	publisher = {National Academies Press},
	organization = {National Academies Press},
	address = {Washington, D.C.},
	keywords = {AUV ABE (Autonomous Benthic Explorer), HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	isbn = {0309089271},
	url = {http://books.nap.edu/openbook.php?record{\_}id=10844{\&}page=97},
	editor = {Board, Ocean Studies}
}
@inbook {Board2003,
	title = {{Related facility needs for an ocean observatories network}},
	booktitle = {Enabling Ocean Research in the 21st Century: Implementation of a Network of Ocean Observatories},
	year = {2003},
	pages = {138{\textendash}156},
	publisher = {National Academies Press},
	organization = {National Academies Press},
	address = {Washington, D.C.},
	keywords = {AUV ABE (Autonomous Benthic Explorer), ROV Jason (Remotely Operated Vehicle)},
	isbn = {0309089905},
	url = {http://books.nap.edu/openbook.php?record{\_}id=10775{\&}page=138},
	editor = {Board, Ocean Studies}
}
@article {Levin2003,
	title = {{Spatial heterogeneity of macrofauna at northern California methane seeps: Influence of sulfide concentration and fluid flow}},
	journal = {Marine Ecology Progress Series},
	volume = {265},
	year = {2003},
	pages = {123{\textendash}139},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0171-8630},
	doi = {10.3354/meps265123},
	url = {http://dx.doi.org/10.3354/meps265123},
	author = {Levin, L A and Ziebis, W and Mendoza, G F and Growney, V A and Tryon, M D and Brown, K M and Mahn, C and Gieskes, J M and Rathburn, A E}
}
@article {Pizarro2003,
	title = {{Toward large-area mosaicing for underwater scientific applications}},
	journal = {IEEE Journal of Oceanic Engineering},
	volume = {28},
	number = {4},
	year = {2003},
	pages = {651{\textendash}672},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0364-9059},
	doi = {10.1109/JOE.2003.819154},
	url = {http://dx.doi.org/10.1109/JOE.2003.819154},
	author = {Pizarro, O and Singh, H}
}
@article {Pruis2002,
	title = {{Age dependent porosity of young upper oceanic crust: Insights from seafloor gravity studies of recent volcanic eruptions}},
	journal = {Geophysical Research Letters},
	volume = {29},
	number = {5},
	year = {2002},
	pages = {1076},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0094-8276},
	doi = {10.1029/2001GL013977},
	url = {http://dx.doi.org/10.1029/2001GL013977},
	author = {Pruis, M J and Johnson, H P}
}
@article {Tivey2002,
	title = {{Crustal magnetization reveals subsurface structure of Juan de Fuca Ridge hydrothermal fields}},
	journal = {Geology},
	volume = {30},
	number = {11},
	year = {2002},
	pages = {979{\textendash}982},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0091-7613},
	doi = {10.1130/0091-7613(2002)030<0979:CMRSSO>2.0.CO;2},
	url = {http://dx.doi.org/10.1130/0091-7613(2002)030{\%}3C0979:CMRSSO{\%}3E2.0.CO 2},
	author = {Tivey, M A and Johnson, H P}
}
@article {Reysenbach2002,
	title = {{Expanding the distribution of the Aquificales to the deep-sea vents on Mid-Atlantic Ridge and Central Indian Ridge}},
	journal = {Cahiers de Biologie Marine},
	volume = {43},
	number = {3-4},
	year = {2002},
	pages = {425{\textendash}428},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0007-9723},
	url = {http://cbm-online.sb-roscoff.fr/pdf/cb43-3-425-428.pdf},
	author = {Reysenbach, A -L and Gotz, D and Banta, A and Jeanthon, C and Fouquet, Y}
}
@article {Biscoito2002,
	title = {{Fishes from the hydrothermal vents and cold seeps: An update}},
	journal = {Cahiers de Biologie Marine},
	volume = {43},
	number = {3-4},
	year = {2002},
	pages = {359{\textendash}362},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0007-9723},
	url = {http://cbm-online.sb-roscoff.fr/pdf/cb43-3-359-362.pdf},
	author = {Biscoito, M and Segonzac, M and Almeida, A J and Desbruyeres, D and Geistdoerfer, P and Turnipseed, M and Van Dover, C L}
}
@article {Humphris2002,
	title = {{Geotectonic setting of hydrothermal activity on the summit of Lucky Strike Seamount (37 degrees 17{\textquoteright}N, Mid-Atlantic Ridge)}},
	journal = {Geochemistry, Geophysics, Geosystems},
	volume = {3},
	number = {8},
	year = {2002},
	pages = {Article no. 1049},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Argo (Remotely Operated Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {1525-2027},
	doi = {10.1029/2001GC000284},
	url = {http://dx.doi.org/10.1029/2001GC000284},
	author = {Humphris, S E and Fornari, D J and Schreirer, D S and German, C R and Parson, L M}
}
@article {Petitt2002,
	title = {{The Hawaii-2 Observatory}},
	journal = {IEEE Journal of Oceanic Engineering},
	volume = {27},
	number = {2},
	year = {2002},
	pages = {245{\textendash}253},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0364-9059},
	doi = {10.1109/JOE.2002.1002479},
	url = {http://dx.doi.org/10.1109/JOE.2002.1002479},
	author = {Petitt, R A and Harris, D W and Wooding, B and Bailey, J and Jolly, J and Hobart, E and Chave, A D and Duennebier, F K and Butler, R and Bowen, A and Yoerger, D R}
}
@article {Levin2002,
	title = {{Isotopic evidence for chemosynthesis-based nutrition of macrobenthos: The lightness of being at Pacific methane seeps}},
	journal = {Limnology and Oceanography},
	volume = {47},
	number = {5},
	year = {2002},
	pages = {1336{\textendash}1345},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0024-3590},
	doi = {10.4319/lo.2002.47.5.1336},
	url = {http://www.aslo.org/lo/toc/vol{\_}47/issue{\_}5/1336.pdf},
	author = {Levin, L A and Michener, R H}
}
@book {Lerner2002,
	title = {{The Jason II virtual control van system, data acquisition system, web-based event logger, and SeaNet}},
	series = {WHOI technical reports},
	volume = {2002-12},
	year = {2002},
	pages = {17},
	publisher = {Woods Hole Oceanographic Institution},
	organization = {Woods Hole Oceanographic Institution},
	address = {Woods Hole, Mass.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1575/1912/27},
	url = {http://hdl.handle.net/1912/27},
	author = {Lerner, S and Maffei, A}
}
@article {VanDover2002a,
	title = {{Trophic relationships among invertebrates at the Kairei hydrothermal vent field (Central Indian Ridge)}},
	journal = {Marine Biology},
	volume = {141},
	number = {4},
	year = {2002},
	pages = {761{\textendash}772},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0025-3162},
	doi = {10.1007/s00227-002-0865-y},
	url = {http://dx.doi.org/10.1007/s00227-002-0865-y},
	author = {Van Dover, C L}
}
@article {Moore2002,
	title = {{Upside-down swimming behavior in a whipnose anglerfish (Teleostei: Ceratioidei: Gigantactinidae)}},
	journal = {Copeia},
	volume = {2002},
	number = {4},
	year = {2002},
	pages = {1144{\textendash}1146},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0045-8511},
	doi = {10.1643/0045-8511(2002)002[1144:UDSBIA]2.0.CO;2},
	url = {http://dx.doi.org/10.1643/0045-8511(2002)002[1144:UDSBIA]2.0.CO 2},
	author = {Moore, J A}
}
@article {Butterfield2001,
	title = {{Evidence for basaltic Sr in midocean ridge-flank hydrothermal systems and implications for the global oceanic Sr isotope balance}},
	journal = {Geochimica et Cosmochimica Acta},
	volume = {65},
	number = {22},
	year = {2001},
	pages = {4141{\textendash}4153},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0016-7037},
	doi = {10.1016/S0016-7037(01)00712-8},
	url = {http://dx.doi.org/10.1016/S0016-7037(01)00712-8},
	author = {Butterfield, D A and Nelson, B K and Wheat, C G and Mottl, M J and Roe, K K}
}
@article {Kelley2001,
	title = {{Geology and venting characteristics of the Mothra hydrothermal field, Endeavour segment, Juan de Fuca Ridge}},
	journal = {Geology},
	volume = {29},
	number = {10},
	year = {2001},
	pages = {959{\textendash}962},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0091-7613},
	doi = {10.1130/0091-7613(2001)029<0959:GAVCOT>2.0.CO;2},
	url = {http://dx.doi.org/10.1130/0091-7613(2001)029{\%}3C0959:GAVCOT{\%}3E2.0.CO 2},
	author = {Kelley, D S and Delaney, J R and Yoerger, D R}
}
@article {Chadwick2001,
	title = {{High-resolution bathymetric surveys using scanning sonars: Lava flow morphology, hydrothermal vent and geologic structure at recent eruption sites on the Juan de Fuca Ridge}},
	journal = {Journal of Geophysical Research},
	volume = {106},
	number = {B8},
	year = {2001},
	pages = {16,16{\textendash}75,100},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0148-0227},
	doi = {10.1029/2001JB000297},
	url = {http://dx.doi.org/10.1029/2001JB000297},
	author = {Chadwick, W W and Schreirer, D S and Embley, R W and Johnson, H P}
}
@inbook {Singh2000b,
	title = {{Advances in fusion of high resolution underwater optical and acoustic data}},
	booktitle = {Proceedings of the 2000 International Symposium on Underwater Technology, Tokyo, Japan, May 23-26, 2000},
	year = {2000},
	pages = {206{\textendash}211},
	publisher = {IEEE},
	organization = {IEEE},
	address = {Piscatway, N.J.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	isbn = {0-7803-6378-7},
	doi = {10.1109/UT.2000.852544},
	url = {http://dx.doi.org/10.1109/UT.2000.852544},
	author = {Singh, H and Roman, C and Whitcomb, L and Yoerger, D R}
}
@inbook {Whitcomb2000,
	title = {{Advances in underwater robot vehicles for deep ocean exploration: Navigation, control, and survey operations}},
	booktitle = {Robotics research: the Ninth International Symposium},
	year = {2000},
	pages = {1{\textendash}9},
	publisher = {Springer},
	organization = {Springer},
	address = {New York},
	keywords = {ROV Argo (Remotely Operated Vehicle), ROV DSL-120 (Remotely Operated Vehicle), ROV Jason (Remotely Operated Vehicle)},
	isbn = {1-85233-292-1},
	author = {Whitcomb, L and Yoerger, D R and Singh, H and Howland, J},
	editor = {Hollerbach, J M}
}
@article {Charlou2000,
	title = {{Compared geochemical signatures and the evolution of Menez Gwen (37 degrees 50{\textquoteright}N) and Lucky Strike (37 degrees17{\textquoteright}N) hydrothermal fluids, south of the Azores triple junction on the Mid-Atlantic Ridge}},
	journal = {Chemical Geology},
	volume = {171},
	number = {1-2},
	year = {2000},
	pages = {49{\textendash}75},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0009-2541},
	doi = {10.1016/S0009-2541(00)00244-8},
	url = {http://dx.doi.org/10.1016/S0009-2541(00)00244-8},
	author = {Charlou, J L and Donval, J P and Douville, E and Jean-Baptiste, P and Radford-Knoery, J and Fouquet, Y and Dapoigny, A and Stievenard, M}
}
@article {Ballard2000a,
	title = {{The discovery of ancient history in the deep sea using advanced deep submergence technology}},
	journal = {Deep-Sea Research. Part I: Oceanographic Research Papers},
	volume = {47},
	number = {9},
	year = {2000},
	pages = {1591{\textendash}1620},
	keywords = {ROV Argo (Remotely Operated Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0967-0637},
	doi = {10.1016/S0967-0637(99)00117-X},
	url = {http://dx.doi.org/10.1016/S0967-0637(99)00117-X},
	author = {Ballard, R D and McCann, A M and Yoerger, D R and Whitcomb, L and Mindell, D and Oleson, J and Singh, H and Foley, B and Adams, J and Piechota, D and Giangrande, C}
}
@article {Johnson2000,
	title = {{Earthquake-induced changes in a hydrothermal system on the Juan de Fuca mid-ocean ridge}},
	journal = {Nature},
	volume = {407},
	number = {6801},
	year = {2000},
	pages = {174{\textendash}177},
	publisher = {Macmillian Magazines Ltd},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0028-0836},
	doi = {10.1038/35025040},
	url = {http://dx.doi.org/10.1038/35025040},
	author = {Johnson, H P and Hutnak, M and Dziak, R P and Fox, C G and Urcuyo, I and Cowen, J P and Nabelek, J and Fisher, C R}
}
@inbook {Bowen2000,
	title = {{Elevators {\textendash} autonomous transporters for deepsea benthic sample recovery}},
	booktitle = {Oceans 2000 MTS/IEEE: where marine science and technology meet},
	volume = {1},
	year = {2000},
	pages = {343{\textendash}348},
	publisher = {IEEE},
	organization = {IEEE},
	address = {Piscataway, N.J.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	isbn = {0-7803-6551-8},
	doi = {10.1109/OCEANS.2000.881282},
	url = {http://dx.doi.org/10.1109/OCEANS.2000.881282},
	author = {Bowen, M F and Bernard, P J and Gleason, D E and Whitcomb, L L}
}
@article {Yoerger2000a,
	title = {{Fine-scale three-dimensional mapping of a deep-sea hydrothermal vent site using the Jason ROV system}},
	journal = {International Journal of Robotics Research},
	volume = {19},
	number = {11},
	year = {2000},
	pages = {1000{\textendash}1014},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0278-3649},
	doi = {10.1177/02783640022067931},
	url = {http://dx.doi.org/10.1177/02783640022067931},
	author = {Yoerger, D R}
}
@article {Singh2000,
	title = {{Microbathymetric mapping from underwater vehicles in the deep ocean}},
	journal = {Computer Vision and Image Understanding},
	volume = {79},
	number = {1},
	year = {2000},
	pages = {143{\textendash}161},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {1077-3142},
	doi = {10.1006/cviu.2000.0850},
	url = {http://dx.doi.org/10.1006/cviu.2000.0850},
	author = {Singh, H and Whitcomb, L and Yoerger, D R and Pizarro, O}
}
@article {Chave2000,
	title = {{Putting H20 in the ocean}},
	journal = {Oceanus},
	volume = {42},
	number = {1},
	year = {2000},
	pages = {6{\textendash}9},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0029-8182},
	author = {Chave, A D and Duennebier, F K and Butler, R}
}
@mastersthesis {Snow1999,
	title = {{Advances in grasping and vehicle contact identification : analysis, design and testing of robust methods for underwater robot manipulation}},
	volume = {Ph.D.},
	year = {1999},
	pages = {228},
	school = {Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution},
	type = {phd},
	address = {Cambridge, MA and Woods Hole, MA},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1575/1912/4695},
	url = {http://hdl.handle.net/1912/4695},
	author = {Snow, E R}
}
@article {Scott1999,
	title = {{Carbon dioxide use by chemoautotrophic endosymbionts of hydrothermal vent vestimentiferans: affinities for carbon dioxide, absence of carboxysomes, and delta C-13 values}},
	journal = {Marine Biology},
	volume = {135},
	number = {1},
	year = {1999},
	month = {oct},
	pages = {25{\textendash}34},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0025-3162},
	doi = {10.1007/s002270050597},
	author = {Scott, K M and Bright, M and Macko, S A and Fisher, C R}
}
@article {Howland1999,
	title = {{Digital mosaicking of underwater imagery}},
	journal = {Sea Technology},
	volume = {40},
	number = {6},
	year = {1999},
	pages = {65{\textendash}69},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0093-3651},
	author = {Howland, J C and Singh, H and Marra, M and Potter, D}
}
@book {Davis1998,
	title = {{Automated analysis of zooplankton size and taxonomic composition}},
	year = {1998},
	publisher = {Woods Hole Oceanographic Instutution},
	organization = {Woods Hole Oceanographic Instutution},
	address = {Woods Hole, Mass.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	url = {http://handle.dtic.mil/100.2/ADA348771},
	author = {Davis, C S and Gallager, S M and Stewart, K}
}
@article {VonDamm1998,
	title = {{The geochemical controls on vent fluids from the Lucky Strike vent field, Mid-Atlantic Ridge}},
	journal = {Earth and Planetary Science Letters},
	volume = {160},
	number = {3-4},
	year = {1998},
	pages = {521{\textendash}536},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0012-821X},
	doi = {10.1016/S0012-821X(98)00108-3},
	url = {http://dx.doi.org/10.1016/S0012-821X(98)00108-3},
	author = {Von Damm, K L and Bray, A M and Buttermore, L G and Oosting, S E}
}
@article {Bachmayer1998,
	title = {{Oceanographic research using remotely operated underwater robotic vehicles: exploration of hydrothermal vent sites on the Mid-Atlantic Ridge at 37 North 32 West}},
	journal = {Marine Technology Society Journal},
	volume = {32},
	number = {3},
	year = {1998},
	pages = {37{\textendash}47},
	keywords = {ROV Argo (Remotely Operated Vehicle), ROV DSL-120 (Remotely Operated Vehicle), ROV Jason (Remotely Operated Vehicle), ROV Medea (Remotely Operated Vehicle)},
	issn = {0025-3324},
	author = {Bachmayer, R and Humphris, S E and Fornari, D J and Van Dover, C L and Howland, J and Bowen, A and Elder, R and Crook, T and Gleason, D E and Sellers, W and Lerner, S}
}
@article {Ballard1998,
	title = {{Oceans of Earth and beyond; the JASON Project IX}},
	journal = {Geotimes},
	volume = {43},
	number = {6},
	year = {1998},
	pages = {14{\textendash}17},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0016-8556},
	author = {Ballard, R D}
}
@article {Sayers1998,
	title = {{Teleprogramming for subsea teleoperation using acoustic communication}},
	journal = {IEEE Journal of Oceanic Engineering},
	volume = {23},
	number = {1},
	year = {1998},
	pages = {60{\textendash}71},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0364-9059},
	doi = {10.1109/48.659450},
	url = {http://dx.doi.org/10.1109/48.659450},
	author = {Sayers, C P and Paul, R P and Whitcomb, L L and Yoerger, D R}
}
@book {Bowen1998a,
	title = {{Ultimate ocean depth packaging for a digital ring laser gyroscope}},
	series = {WHOI technical reports},
	volume = {98-15},
	year = {1998},
	pages = {24},
	publisher = {Woods Hole Oceanographic Institution},
	organization = {Woods Hole Oceanographic Institution},
	address = {Woods Hole, Mass.},
	keywords = {AUV ABE (Autonomous Benthic Explorer), HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	url = {http://hdl.handle.net/1912/323},
	author = {Bowen, M F}
}
@article {Fornari1997,
	title = {{Inductively coupled link (ICL) temperature probes for hot hydrothermal fluid sampling from ROV Jason and DSV Alvin}},
	journal = {RIDGE Events},
	volume = {8},
	number = {1},
	year = {1997},
	pages = {26{\textendash}30},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	url = {http://www.ridge2000.org/science/downloads/newsletters/R2KNews0297.pdf},
	author = {Fornari, D J and Bradley, A and Humphris, S E and Walden, B and Deuster, A}
}
@article {Goff1997,
	title = {{Multiscale spectral analysis of bathymetry on the flank of the Mid-Atlantic Ridge: Modification of the seafloor by mass wasting and sedimentation}},
	journal = {Journal of Geophysical Research},
	volume = {102},
	number = {B7},
	year = {1997},
	pages = {15,415{\textendash}447,462},
	keywords = {ROV DSL-120 (Remotely Operated Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0148-0227},
	doi = {10.1029/97JB00723},
	url = {http://dx.doi.org/10.1029/97JB00723},
	author = {Goff, J A and Tucholke, B E}
}
@inbook {Singh1996,
	title = {{An entropic framework for sensor modelling}},
	booktitle = {Oceans {\textquoteright}96 MTS/IEEE : conference proceedings, 23-26 September, 1996, Broward County Convention Center},
	volume = {1},
	year = {1996},
	pages = {211{\textendash}215},
	publisher = {IEEE},
	organization = {IEEE},
	address = {Piscataway, N.J.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	isbn = {0-7803-3520-1},
	doi = {10.1109/OCEANS.1996.572604},
	url = {http://dx.doi.org/10.1109/OCEANS.1996.572604},
	author = {Singh, H and Stewart, W K}
}
@mastersthesis {Knowles1996,
	title = {{Experiments and numerical simulations of the dynamics of an R.O.V. thruster during maneuvering}},
	volume = {M.S.},
	year = {1996},
	pages = {71},
	school = {Massachusetts Institute of Technology and Woods Hole Oceanographic Institution},
	type = {phd},
	address = {Cambridge, Mass. and Woods Hole, Mass.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	url = {http://hdl.handle.net/1721.1/38180},
	author = {Knowles, J H}
}
@article {Fornari1995,
	title = {{Visualizing the deep sea - vehicles and research techniques yield ever-clearer images of the ocean depths}},
	journal = {Oceanus},
	volume = {38},
	number = {1},
	year = {1995},
	pages = {10{\textendash}13},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Argo (Remotely Operated Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0029-8182},
	author = {Fornari, D J and Bowen, A M and Foster, D B}
}
@inbook {Bowen1994,
	title = {{The Argo/Jason ROV development program, past and present challenges}},
	booktitle = {Diving for science...1994 : proceedings of the American Academy of Underwater Sciences fourteenth annual scientific diving symposeum, October 5-9, 1994},
	year = {1994},
	publisher = {American Academy of Underwater Sciences},
	organization = {American Academy of Underwater Sciences},
	address = {Costa Mesa, CA},
	keywords = {ROV Argo (Remotely Operated Vehicle), ROV Jason (Remotely Operated Vehicle)},
	author = {Bowen, A}
}
@book {McCann1994,
	title = {{Deep water archaeology: a late Roman ship sailing from Carthage and a trade route northeast of Skerki Bank}},
	series = {Journal of Roman Archaeology: Supplements},
	number = {13},
	year = {1994},
	pages = {162},
	publisher = {Journal of Roman Archaeology},
	organization = {Journal of Roman Archaeology},
	address = {Ann Arbor, Mich.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	isbn = {1-887829-13-X},
	author = {McCann, A M and Freed, J}
}
@inbook {Garzke1993,
	title = {{Deep underwater exploration vehicles {\textendash} past, present and future}},
	booktitle = {Centennial meeting, technical sessions, September 15-17, 1992, the Society of Naval Architects and Marine Engineers, the New York Hilton and Towers, New York, NY},
	year = {1993},
	pages = {15{\textendash}40},
	publisher = {Society of Naval Architects and Marine Engineers},
	organization = {Society of Naval Architects and Marine Engineers},
	address = {Jersey City, N.J.},
	keywords = {ROV Argo (Remotely Operated Vehicle), ROV Jason (Remotely Operated Vehicle)},
	author = {Garzke, W H and Yoerger, D R and Harris, S and Dulin, R O and Brown, D K}
}
@article {Travis1993,
	title = {{Deep-sea debate pits Alvin against Jason}},
	journal = {Science},
	volume = {259},
	number = {5101},
	year = {1993},
	pages = {1534{\textendash}1536},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0036-8075},
	doi = {10.1126/science.259.5101.1534},
	url = {http://dx.doi.org/10.1126/science.259.5101.1534},
	author = {Travis, J}
}
@article {Ballard1993a,
	title = {{Fine-scale mapping programs using Jason ROV system}},
	journal = {EOS, Transactions, American Geophysical Union},
	volume = {74},
	number = {43 (Fall Meet. Suppl.)},
	year = {1993},
	pages = {561},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0096-3941},
	author = {Ballard, R D}
}
@article {Robigou1993a,
	title = {{Jason Project IV; combined operations of Jason ROV, Turtle submersible and satellite link to land}},
	journal = {EOS, Transactions, American Geophysical Union},
	volume = {74},
	number = {43 (Fall Meet. Suppl.)},
	year = {1993},
	pages = {573},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0096-3941},
	author = {Robigou, V}
}
@book {Ballard1993b,
	title = {{The Jason remotely operated vehicle system}},
	series = {WHOI technical reports},
	volume = {93-34},
	year = {1993},
	pages = {47},
	publisher = {Woods Hole Oceanographic Institution},
	organization = {Woods Hole Oceanographic Institution},
	address = {Woods Hole, Mass.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1575/1912/633},
	url = {http://hdl.handle.net/1912/633},
	author = {Ballard, R D}
}
@inbook {Mindell1993,
	title = {{Jason talk: a standard ROV vehicle control system}},
	booktitle = {Oceans {\textquoteright}93: Engineering in harmony with the ocean: Proceedings},
	volume = {3},
	year = {1993},
	pages = {III253{\textendash}III258},
	publisher = {IEEE},
	organization = {IEEE},
	address = {New York, N.Y.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	isbn = {0-7803-1385-2},
	author = {Mindell, D A and Yoerger, D R and Freitag, L E and Whitcomb, L L and Eastwood, R L}
}
@article {Ballard1993,
	title = {{The Medea/Jason remotely operated vehicle system}},
	journal = {Deep-Sea Research. Part I: Oceanographic Research Papers},
	volume = {40},
	number = {8},
	year = {1993},
	pages = {1673{\textendash}1687},
	keywords = {ROV Jason (Remotely Operated Vehicle), ROV Medea (Remotely Operated Vehicle)},
	issn = {0967-0637},
	doi = {10.1016/0967-0637(93)90021-T},
	url = {http://dx.doi.org/10.1016/0967-0637(93)90021-T},
	author = {Ballard, R D}
}
@book {Howland1993,
	title = {{Near real-time GIS in deep-ocean exploration}},
	year = {1993},
	pages = {8},
	publisher = {Woods Hole Oceanographic Institution},
	organization = {Woods Hole Oceanographic Institution},
	address = {Woods Hole, Mass.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	url = {http://handle.dtic.mil/100.2/ADA262073},
	author = {Howland, J C and Marra, M and Potter, D F and Stewart, W K}
}
@inbook {Whitcomb1993,
	title = {{A new distributed real-time control system for the Jason underwater robot}},
	booktitle = {IROS {\textquoteright}93: Proceedings of the IEEE/RSJ International Conference on Intelligent Robots and Systems: Intelligent Robots for Flexibility},
	volume = {1},
	year = {1993},
	pages = {368{\textendash}374},
	publisher = {IEEE},
	organization = {IEEE},
	address = {New York, N.Y.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	isbn = {0-7803-0824-7},
	doi = {10.1109/IROS.1993.583124},
	url = {http://dx.doi.org/10.1109/IROS.1993.583124},
	author = {Whitcomb, L L and Yoerger, D R}
}
@article {McDuff1993,
	title = {{Observations of entrainment into a rising hydrothermal plume with the remotely operated vehicle Jason}},
	journal = {EOS, Transactions, American Geophysical Union},
	volume = {74},
	number = {43 (Fall Meet. Suppl.)},
	year = {1993},
	pages = {573},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0096-3941},
	author = {McDuff, R E}
}
@conference {Elder1992,
	title = {{Converting Jason Junior, a small ROV, to fiber optics}},
	booktitle = {Intervention/ROV {\textquoteright}92 conference {\&} exposition: a decade of achievement},
	volume = {San Diego,},
	year = {1992},
	pages = {479{\textendash}484},
	publisher = {Marine Technology Society},
	organization = {Marine Technology Society},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	author = {Elder, R L}
}
@article {Hover1992a,
	title = {{Identification of low-order dynamic models for deeply towed underwater vehicle systems}},
	journal = {International Journal of Offshore and Polar Engineering},
	volume = {2},
	number = {1},
	year = {1992},
	pages = {38{\textendash}45},
	keywords = {ROV Jason (Remotely Operated Vehicle), ROV Medea (Remotely Operated Vehicle)},
	issn = {1053-5381},
	author = {Hover, F S and Yoerger, D R}
}
@mastersthesis {Malmquist1992,
	title = {{Modeling a 300 kHz bathymetric sonar system}},
	volume = {M.S.},
	year = {1992},
	pages = {124},
	school = {Massachusetts Institute of Technology and Woods Hole Oceanographic Institution},
	type = {phd},
	address = {Cambridge and Woods Hole, Mass.},
	keywords = {ROV Argo (Remotely Operated Vehicle), ROV Jason (Remotely Operated Vehicle)},
	url = {http://handle.dtic.mil/100.2/ADA257378},
	author = {Malmquist, K A}
}
@inbook {Howland1992,
	title = {{Near real-time GIS in deep-ocean exploration}},
	booktitle = {ASPRS/ACSM/RT 92 technical papers},
	year = {1992},
	pages = {428{\textendash}435},
	publisher = {American Society for Photogrammetry and Remote Sensing; American Congress on Surveying and Mapping},
	organization = {American Society for Photogrammetry and Remote Sensing; American Congress on Surveying and Mapping},
	address = {Bethesda, Md.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	isbn = {0-9444268-3-2},
	author = {Howland, J C and Marra, M and Potter, D F and Stewart, W K}
}
@article {Sigurdsson1992,
	title = {{Working with Jason in the Galapagos}},
	journal = {Maritimes},
	volume = {36},
	number = {1},
	year = {1992},
	pages = {13{\textendash}15},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0025-3472},
	author = {Sigurdsson, H}
}
@inbook {Ballard1991,
	title = {{Argo/Jason: A remotely operated survey and sampling system for full-ocean depth}},
	booktitle = {Oceans {\textquoteright}91: Proceedings, October 1-3, 1991, Honolulu, Hawaii, USA : Ocean Technologies and Opportunities in the Pacific for the 90{\textquoteright}s},
	volume = {1},
	year = {1991},
	pages = {71{\textendash}75},
	publisher = {IEEE},
	organization = {IEEE},
	address = {New York},
	keywords = {ROV Argo (Remotely Operated Vehicle), ROV Jason (Remotely Operated Vehicle)},
	isbn = {0-7803-0202-8},
	doi = {10.1109/OCEANS.1991.613909},
	url = {http://dx.doi.org/10.1109/OCEANS.1991.613909},
	author = {Ballard, R D and Yoerger, D R and Stewart, W K and Bowen, A}
}
@article {Yoerger1991,
	title = {{Design and performance evaluation of an actively compliant underwater manipulator for full-ocean depth}},
	journal = {Journal of Robotic Systems},
	volume = {8},
	number = {3},
	year = {1991},
	pages = {371{\textendash}392},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0741-2223},
	doi = {10.1002/rob.4620080306},
	url = {http://dx.doi.org/10.1002/rob.4620080306},
	author = {Yoerger, D R and Schempf, H and DiPietro, D M}
}
@inbook {Elder1991,
	title = {{Fiber optic telemetry in Jason, the ROV}},
	booktitle = {Intervention/ROV {\textquoteright}91 Conference {\&} Exposition: May 21-23, 1991, Diplomat Resort and Convention Center, Hollywood, Florida},
	year = {1991},
	pages = {78{\textendash}84},
	publisher = {Marine Technology Society ROV Committee},
	organization = {Marine Technology Society ROV Committee},
	address = {San Diego, Calif.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	author = {Elder, R L}
}
@article {Stewart1991b,
	title = {{High-resolution optical and acoustic remote sensing for underwater exploration}},
	journal = {Oceanus},
	volume = {34},
	number = {1},
	year = {1991},
	pages = {10{\textendash}22},
	keywords = {ROV Argo (Remotely Operated Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0029-8182},
	author = {Stewart, W K}
}
@article {Yoerger1991a,
	title = {{Robotic undersea technology}},
	journal = {Oceanus},
	volume = {34},
	number = {1},
	year = {1991},
	pages = {32{\textendash}37},
	keywords = {AUV ABE (Autonomous Benthic Explorer), ROV Jason (Remotely Operated Vehicle), ROV Medea (Remotely Operated Vehicle)},
	issn = {0029-8182},
	author = {Yoerger, D R}
}
@inbook {McCann1991,
	title = {{ROVs for archaeology: The Jason Project 1989}},
	booktitle = {Intervention/ROV {\textquoteright}91 Conference {\&} Exposition: May 21-23, 1991, Diplomat Resort and Convention Center, Hollywood, Florida},
	year = {1991},
	pages = {13},
	publisher = {Marine Technology Society ROV Committee},
	organization = {Marine Technology Society ROV Committee},
	address = {San Diego, Calif.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	author = {McCann, A M}
}
@book {Triantafyllou1990,
	title = {{Cable dynamics for tethered underwater vehicles}},
	series = {MITSG},
	year = {1990},
	pages = {32},
	publisher = {Sea Grant College Program, Massachusetts Institute of Technology},
	organization = {Sea Grant College Program, Massachusetts Institute of Technology},
	address = {Cambridge, Mass.},
	keywords = {ROV Argo (Remotely Operated Vehicle), ROV Jason (Remotely Operated Vehicle)},
	url = {http://nsgl.gso.uri.edu/mit/mitt90001.pdf},
	author = {Triantafyllou, M S and Hover, F}
}
@inbook {Yoerger1990,
	title = {{Control capabilities of Jason and its manipulator}},
	booktitle = {Subsea Work Systems and Technologies},
	series = {Ocean Resources},
	number = {2},
	year = {1990},
	pages = {19{\textendash}29},
	publisher = {Kluwer Academic Publishers.},
	organization = {Kluwer Academic Publishers.},
	address = {Boston},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	isbn = {0-7923-0953-7},
	author = {Yoerger, D R and DiPietro, D M},
	editor = {Ardus, D A}
}
@article {Newman1990a,
	title = {{Fiber-optic data network for the Argo/Jason vehicle systems}},
	journal = {IEEE Journal of Oceanic Engineering},
	volume = {15},
	number = {2},
	year = {1990},
	pages = {66{\textendash}71},
	keywords = {ROV Argo (Remotely Operated Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0364-9059},
	doi = {10.1109/48.50691},
	url = {http://dx.doi.org/10.1109/48.50691},
	author = {Newman, J B}
}
@article {Mindell1990,
	title = {{Images from the deep: Using a fiber-optic network and oceanographic vehicles, Woods Hole scientists are probing the wonders of the ocean.}},
	journal = {Byte Magazine},
	volume = {15},
	number = {6},
	year = {1990},
	pages = {256{\textendash}260},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0360-5280},
	author = {Mindell, D A}
}
@book {Newman1990,
	title = {{Jason computers, sensors and software}},
	series = {WHOI technical memoranda},
	volume = {1-90},
	year = {1990},
	pages = {28},
	publisher = {Woods Hole Oceanographic Institution},
	organization = {Woods Hole Oceanographic Institution},
	address = {Woods Hole, Mass.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	author = {Newman, J B}
}
@article {Bowen1990,
	title = {{Jason Med adventure}},
	journal = {Oceanus},
	volume = {33},
	number = {1},
	year = {1990},
	pages = {61{\textendash}69},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0029-8182},
	author = {Bowen, M F}
}
@book {Ballard1990a,
	title = {{The Lost Wreck of the Isis}},
	year = {1990},
	pages = {63},
	publisher = {Scholastic},
	organization = {Scholastic},
	address = {New York},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	isbn = {0-590-43852-2},
	author = {Ballard, R D and Archbold, R}
}
@article {Tyre1989,
	title = {{Live TV broadcasts from ocean floor bring new depth to science education}},
	journal = {Technological Horizons in Education Journal},
	volume = {17},
	number = {1},
	year = {1989},
	pages = {42,44{\textendash}46},
	keywords = {ROV Jason (Remotely Operated Vehicle), ROV Medea (Remotely Operated Vehicle)},
	issn = {0192-592X},
	author = {Tyre, T}
}
@inbook {Yoerger1988,
	title = {{Design and control of Jason}},
	booktitle = {Intervention {\textquoteright}88, April 17-20: Underwater Technology Conference, 1988},
	year = {1988},
	pages = {541{\textendash}553},
	publisher = {Det norske Veritas},
	organization = {Det norske Veritas},
	address = {Bergen, Norway},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	isbn = {8299113423},
	author = {Yoerger, D R and von Alt, C and Newman, J B and Bowen, A and Hersey, W J},
	editor = {Atteraas, L}
}
@article {Uchupi1988a,
	title = {{Geology of the Titanic site and vicinity}},
	journal = {Deep-Sea Research. Part A, Oceanographic Research Papers},
	volume = {35},
	number = {7},
	year = {1988},
	pages = {1093{\textendash}1110},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Argo (Remotely Operated Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0198-0149},
	doi = {10.1016/0198-0149(88)90002-7},
	url = {http://dx.doi.org/10.1016/0198-0149(88)90002-7},
	author = {Uchupi, E and Muck, M T and Ballard, R D}
}
@inbook {VonAlt1987,
	title = {{Jason Junior: System design and deep submergence vehicle interface}},
	booktitle = {Intervention {\textquoteright}87 Conference and Exposition},
	year = {1987},
	pages = {200{\textendash}208},
	publisher = {Marine Technology Society},
	organization = {Marine Technology Society},
	address = {San Diego, Calif.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	author = {von Alt, C and Bowen, A D and Bergeron, E M}
}
@inbook {Newman1987,
	title = {{Jason Junior{\textquoteright}s control system}},
	booktitle = {Intervention {\textquoteright}87 Conference and Exposition},
	year = {1987},
	pages = {194{\textendash}199},
	publisher = {Marine Technology Society},
	organization = {Marine Technology Society},
	address = {San Diego, Calif.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	author = {Newman, J B}
}
@article {Ballard1987a,
	title = {{Live deep-sea expedition video coverage planned for scientists ashore}},
	journal = {Oceanus},
	volume = {30},
	number = {3},
	year = {1987},
	pages = {11{\textendash}15},
	keywords = {ROV Argo (Remotely Operated Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0029-8182},
	author = {Ballard, R D and von Alt, C and Hersey, W J}
}
@book {U.S.Congress1987,
	title = {{Marine minerals: Exploring our new ocean frontier}},
	year = {1987},
	pages = {349},
	publisher = {U.S. Government Printing Office},
	organization = {U.S. Government Printing Office},
	address = {Washington, D.C.},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Argo (Remotely Operated Vehicle), ROV Jason (Remotely Operated Vehicle)},
	url = {http://purl.access.gpo.gov/GPO/LPS27719},
	author = {U.S. Congress, Office of Technology Assessment}
}
@inbook {Yoerger1986,
	title = {{Design of underwater vehicles for high performance control}},
	booktitle = {Oceans {\textquoteright}86 Conference Record},
	volume = {5},
	year = {1986},
	pages = {1431{\textendash}1437},
	publisher = {IEEE},
	organization = {IEEE},
	address = {New York, N.Y.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	doi = {10.1109/OCEANS.1986.1160335},
	url = {http://dx.doi.org/10.1109/OCEANS.1986.1160335},
	author = {Yoerger, D R and von Alt, C J and Bowen, A D and Newman, J B}
}
@article {Lemonick1986,
	title = {{{\textquoteright}J.J.{\textquoteright} tours the Titanic: Far below the waves, a versatile robot explores the great ship}},
	journal = {Time},
	volume = {128},
	number = {July 28},
	year = {1986},
	pages = {52},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0040-781X},
	url = {http://www.time.com/time/magazine/article/0,9171,961826,00.html},
	author = {Lemonick, M D}
}
@article {950,
	title = {{Picture perfect return to the R.M.S. Titanic}},
	journal = {Sea Technology},
	volume = {27},
	number = {9},
	year = {1986},
	pages = {19{\textendash}22},
	keywords = {HOV Alvin (Human Occupied Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0093-3651}
}
@article {Yoerger1986a,
	title = {{Supervisory control system for the Jason ROV}},
	journal = {IEEE Journal of Oceanic Engineering},
	volume = {11},
	number = {3},
	year = {1986},
	pages = {392{\textendash}400},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	issn = {0364-9059},
	doi = {10.1109/JOE.1986.1145191},
	url = {http://dx.doi.org/10.1109/JOE.1986.1145191},
	author = {Yoerger, D R and Newman, J B and Slotine, J E}
}
@book {102,
	title = {{MIT underwater technology research: Telemanipulator developments}},
	series = {MITSG Opportunity brief (MIT/Marine Industry Collegium)},
	volume = {40},
	year = {1985},
	pages = {15},
	publisher = {Marine Industry Advisory Services, MIT Sea Grant Program},
	organization = {Marine Industry Advisory Services, MIT Sea Grant Program},
	address = {Cambridge, Mass.},
	keywords = {ROV Jason (Remotely Operated Vehicle)}
}
@inbook {Yoerger1984,
	title = {{Man-machine inferface and control concepts for the Jason program}},
	booktitle = {Remotely Operated Vehicles: ROV {\textquoteright}84 Technology Update, an International Perspective : May 14-18, 1984, Town and Country Hotel, San Diego, California},
	year = {1984},
	pages = {227{\textendash}232},
	publisher = {Marine Technology Society},
	organization = {Marine Technology Society},
	address = {San Diego, Calif.},
	keywords = {ROV Jason (Remotely Operated Vehicle)},
	author = {Yoerger, D R}
}
@article {Ballard1982a,
	title = {{Argo and Jason}},
	journal = {Oceanus},
	volume = {25},
	number = {1},
	year = {1982},
	pages = {30{\textendash}35},
	keywords = {ROV Argo (Remotely Operated Vehicle), ROV Jason (Remotely Operated Vehicle)},
	issn = {0029-8182},
	author = {Ballard, R D}
}
